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Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_9 | Pages 65 - 65
1 May 2016
Murakami T Yarimitsu S Nakashima K Sakai N Yamaguchi T Sawae Y Suzuki A
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Total hip and knee joint prostheses composed of ultra-high molecular weight polyethylene (UHMWPE) and metal or ceramics have been widely applied. Efficacious treatments such as crosslinking, addition of vitamin E and phospholipid coating to UHMWPE have reduced wear and extended the life of joint prostheses. However, wear problems have not yet been completely solved for cases involving severe conditions, where direct contact can occur in mixed or boundary lubrication. In contrast, extremely low friction and minimum wear are maintained for a lifetime in healthy natural synovial joints containing articular cartilage with superior lubricity. Accordingly, joint prostheses containing artificial hydrogel cartilage with properties similar to those of articular cartilage are expected to show superior tribological functions. In establishing the function of artificial hydrogel cartilage as a novel material for joint prostheses, the tribological properties of hydrogel materials used and synergistic performance with synovia constituents are both important. In this study, the lubrication ability and wear resistance properties of poly(vinyl alcohol) (PVA) hydrogels were evaluated by differences in friction and wear properties in reciprocating tests lubricated with saline and simulated synovial fluid. Biphasic finite element (FE) analysis was applied to elucidate the role of biphasic lubrication mechanism in hydrogels.

As biocompatible artificial hydrogel cartilage materials, three PVA hydrogels were prepared using the repeated freeze-thawing (FT) method, the cast-drying (CD) method and the hybrid method for laminated gel of FT on CD, which are physically crosslinked with hydrogen bonding but differ in terms of structure and mechanical properties. First the frictional behavior of the ellipsoidal PVA hydrogel specimens was examined in reciprocating tests against a glass plate, which corresponds to simplified knee prosthesis model (Fig.1), with a sliding speed of 20 mm/s under constant continuous loading. As shown in Fig.1, the three hydrogels exhibited different frictional behaviors in a saline solution. It is noteworthy that the hybrid gel maintained very low friction until the end of test. The CD gel showed slightly higher friction and a gradual increase. Meanwhile, the FT gel showed initial medium friction and a gradual increase. Time-dependent frictional behavior was clarified with biphasic lubrication mechanism via biphasic FE analysis. Contact surface observation showed minimal wear without scratches for hybrid gel in saline.

Next, simulated synovial fluid composed of 0.5 wt% hyaluronic acid (HA, molecular weight: 920,000 Da), 1.4 wt% albumin, 0.7 wt% gamma-globulin and 0.01 wt% L-alpha dipalmitoylphosphatidylcholine (DPPC), was used to evaluate tribological performance of these gels in physiological condition. As shown in Fig.2, PVA hydrogels in simulated synovial fluid exhibited very low friction, with hybrid gel showing an extremely low friction coefficient of 0.003 in the test. These friction differences were sustained by biphasic FE analysis. Hybrid gel further showed very little wear (Fig.3), which is favorable in terms of hydrogel durability.

These results indicate the importance of superior lubricity and wear resistance of PVA hybrid gel for the clinical application of artificial hydrogel cartilage in joint prostheses.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_4 | Pages 143 - 143
1 Jan 2016
Yarimitsu S Yoshida A Nakashima K Sawae Y Murakami T Sasaki S Suzuki A
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Poly (vinyl alcohol) (PVA) hydrogel with high water content is one of the potential materials for artificial cartilage. In the previous study, the wear behavior of PVA hydrogel prepared by freeze-thawing (FT) method (PVA-FT gel) showed the excellent friction and wear property in simulated biological environment. However, the improvement of mechanical strength and wear resistance would be also needed for clinical application of PVA hydrogel as artificial cartilage. The different kind of physically-crosslinked PVA hydrogels prepared by cast-drying (CD) method (PVA-CD gel) and hybrid method of FT and CD (PVA-CD on FT hybrid gel) were also developed, and these two hydrogels have different mechanical properties and showed low friction compared with PVA-FT gel in saline. In this study, PVA hydrogel prepared by CD and hybrid methods were newly developed and friction and wear behavior of PVA-CD gel and PVA-CD on FT hybrid gel were evaluated in simulated biological environment.

A sliding pair of an ellipsoidal reciprocating upper specimen of hydrogel and a flat stationary lower specimen of hydrogel was tested in reciprocating friction test. The thicknesses of PVA-CD gel and PVA-CD on FT hybrid gel were 2.0mm and 1.7mm, respectively. The applied load was 2.94 N. The sliding velocity was 20 mm/s and the total sliding distance was 1.5 km. In this study, solutions that contain hyaluronic acid, phospholipid and proteins were prepared as simulated synovial fluid and used as a lubricant for friction test. Molecular weight of sodium hyaluronate was 9.2×105. L-alpha dipalmitoylphosphatidylcholine (DPPC) was selected as phospholipid constituent and was dispersed in saline as liposome. This liposomal solution was used as a base lubricant. Albumin and gamma-globulin, which are main protein constituents in natural synovial fluid, were used as additives as protein constituents.

As shown in Fig.1, PVA-CD gel showed low friction such as below 0.02 at initial state of friction test. However, friction coefficient of PVA-CD gel rapidly increased and reached to about 0.5. In contrast, PVA-CD on FT hybrid gel kept low friction within the friction test. After friction test, many deep scratches were observed on the worn surface of PVA-CD gel (Figs. 2(a)-(c)). In contrast, the original surface structure of PVA-CD on FT hybrid gel almost remained while some scratches were observed (Figs. 2(d)-(f)).

These results indicated that PVA-CD gel could show low friction but low wear resistance. The hybridization of FT and CD improved the wear resistance of PVA-CD gel. Therefore, the hybridization of FT and CD method is one of the prospective preparation methods of artificial cartilage with low friction and low wear. It is important to elucidate the mechanism of excellent lubricating property of PVA-CD on FT hybrid gel and develop the highly-functioned artificial hydrogel cartilage with low friction and high wear resistance.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_3 | Pages 59 - 59
1 Jan 2016
Murakami T Yarimitsu S Nakashima K Yamaguchi T Sawae Y Sakai N Suzuki A
Full Access

In joint prostheses where ultra-high molecular weight polyethylene (UHMWPE) is used as bearing material, efficacious treatments such as crosslinking, addition of vitamin E and the grafting of phospholipid polymer are known to improve wear resistance. Under severe conditions of various daily activities, however, friction and wear problems in such prostheses have not yet been completely solved. In contrast, extremely low friction and minimum wear have been maintained for a lifetime in healthy natural synovial joints containing articular cartilage with superior lubricity. Accordingly, joint prostheses containing artificial hydrogel cartilage with properties similar to those of articular cartilage are expected to show superior tribological functions. In establishing the function of artificial hydrogel cartilage as a novel material for joint prostheses, the tribological properties of hydrogel materials used and synergistic performance with synovia constituents are both important. In this study, the influence of synovia constituents on friction and wear in artificial hydrogels was examined in reciprocating test and compared with that for articular cartilage.

As biocompatible artificial hydrogel cartilage materials, three poly(vinyl alcohol) (PVA) hydrogels were prepared using the repeated freeze-thawing (FT) method, the cast-drying (CD) method and hybrid method for CD on FT, which are physically crosslinked with hydrogen bonding but differ in terms of structure and mechanical properties. First the frictional behavior of the PVA hydrogels and articular cartilage as ellipsoidal specimens was examined in reciprocating tests against a glass plate with a sliding speed of 20 mm/s under constant continuous loading. As shown in Fig.1, the three hydrogels exhibited different frictional behaviors in a saline solution. It is noteworthy that the hybrid gel maintained very low friction until the end of test. The CD gel showed slightly higher friction and a gradual increase. Meanwhile, the FT gel showed initial medium friction and a gradual increase echoing the time-dependent behavior of natural articular cartilage. Based on these observations, focus was placed on FT gel and articular cartilage to examine how synovia constituents influence friction and wear in these hydrogel materials.

In human body, lubricating constituents in synovial fluids such as hyaluronic acid, proteins, glycoproteins and phospholipids are considered to reduce the coefficient of friction in solid-to-solid interaction. Here, the effects of hyaluronic acid (HA, molecular weight: 9.2×105), serum proteins and phospholipid were examined. Dipalmitoylphosphatidylcholine (DPPC) was used as a typical phospholipid. As indicated in Fig.2 for repeated reciprocating tests, addition of HA alone was effective particularly for PVA-FT hydrogel. The combination of HA and DPPC was more effective in reduction of friction. The simulated synovial fluid (composed of HA 0.5 wt%, DPPC 0.01 wt%, albumin(Alb) 1.4 wt% and gamma-globulin (g-glob) 0.7 wt%) exhibited both low friction and minimum wear. The rubbing surfaces of articular cartilage and FT gel after tests are shown in Fig.3. On the articular cartilage surface, gel-like surface layer existed. On the FT gel surface, the original texture was observed without damage.

These results indicate the importance of synovia constituents for the clinical application of artificial hydrogel cartilage in joint prostheses.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_1 | Pages 80 - 80
1 Jan 2016
Cho C Murakami T Sawae Y
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Ultra-high molecular weight polyethylene (UHMWPE) is the sole polymeric material currently used for weight-bearing surfaces in total joint arthroplasty. However, the wear phenomenon of UHMWPE components in knee and hip prostheses after total joint arthroplasty is one of the major restriction factors on the longevity of these implants. In order to minimize the wear of UHMWPE and to improve the longevity of artificial joints, it is necessary to clarify the factors influencing the wear mechanism of UHMWPE. In the microscopic surface observation of the virgin knee prosthesis with anatomical design, various grades of microscopic surface scratches and defects caused by machining and surface finishing processes during manufacture of the component were observed on the surface of the metallic femoral component [Fig. 1] (C. Cho et al, 2009), although the overall surface were finished at smoother level. It is thought that certain levels of the microscopic surface asperities caused by these surface damages in the metallic femoral component might contribute to increasing and/or accelerating wear of the UHMWPE tibial insert. Therefore, it is necessary to clarify quantitatively the influence of the microscopic surface asperities of the metallic components in virgin artificial joints on the wear of UHMWPE components.

The primary purpose of this study was to investigate the influence of the microscopic surface asperities of the virgin metallic femoral component on the wear of the UHMWPE tibial insert in the virgin knee prosthesis. In this study, the authors focused on the three-dimensional shape of the microscopic surface asperities as a factor influencing the wear mechanism of the UHMWPE tibial insert. The 3D microscopic surface profile measurement of the virgin metallic femoral component using a laser microscope and reproduction of the femoral component surface using 3D CAD software were performed [Fig. 2] in order to produce idealized 3D finite element models of the microscopic surface asperity of the femoral component based on actual measurement data. Elasto-plastic finite element contact analyses between idealized microscopic surface asperities and UHMWPE were also performed in order to investigate the influence of the three-dimensional shape of the microscopic surface asperities of the virgin metallic femoral component on the wear of the UHMWPE tibial insert. The analytical findings of this study suggest that the aspect ratio and shape ratio [Fig. 3] of the microscopic surface asperity of the virgin metallic femoral component have an important influence on increasing and/or accelerating wear of the UHMWPE tibial insert.


Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_3 | Pages 103 - 103
1 Jan 2016
Omata S Sawae Y Murakami T
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It is reported that more than 10 million Japanese suffer from arthrosis. To cure these cartilage defects, total joint replacements, which are the most popular treatment methods for severe disease situation, have been operated as about two hundred thousand cases a year in Japan. Although the implants made of either ceramics, metals or plastics have high wear resistance quality, it becomes apparent that the endurance life of the artificial joints in considerable cases is limited by aseptic loosening to between 10–15 years. Here we focused on a poly(vinyl alcohol) (PVA) hydrogel as an artificial cartilage tissue to make an improvement of friction surface of the artificial joints. In this paper, we observed morphology of wear particles and assessed immune responses of wear particles from the hydrogel for confirming the validity of the gel as a biotribological material.

We prepared 20 w/w% of PVA hydrogel by repeated freezing-thawing method. The number of the freezing-thawing cycles was five times. Polymerization degree and saponification degree of PVA (Kishida Chemical Co. Ltd., Japan) were 2000 and 98.4–99.8 mol%, respectively. To collect the wear particles of PVA hydrogel, we processed wear testing by using a purpose-build wear test machine of reciprocating pin-on-plate tribometer as shown in figure 1. We installed a Co-Cr-Mo ball of 26 mm in diameter as a stationary upper specimen and a PVA hydrogel plate of 2 mm in thickness as reciprocating lower specimen in a water bath. The lubricant was a distilled water containing eluted PVA which PVA-FT gel had been soaked in, filtered by 0.22 μm and autoclaved, subsequently. Siding speed was 50 mm/s and the total sliding distance was 3 km. We observed the wear particles which had been dried in a desiccator, by scanning electron microscope (SEM; SU8000, Hitachi High-Technologies). Additionally, to investigate the effect of the wear particles on response of phagocytosis of macrophages, here we used THP-1 cell line from Human acute monocytic leukemia as a macrophage, which was purchased from JCRB Cell Bank, and attached the macrophages on a dish after stimulating THP-1 by phorbol 12-myristate 13-acetate (PMA; Wako). After the wear test, we harvested the lubricant in aseptic clean hood and applied the particles to the macrophages to clarify the effect of wear particles of PVA hydrogel on immune response of the cell. To assess cytokine biosynthesis as immune responses, we assayed IL-1β and TNF-α biosynthesis in culture medium by ELISA (Thermo scientific), respectively.

Figure 2 shows an SEM image of PVA hydrogel after wear test under 4.9 N loading. We observed the wear particles of varied sizes. When applying the wear particles to the macrophages in RPMI-1640 supplement with 10 v/v% fetal bovine serum, it seemed that there were not enough change on cytokine synthesis in culture medium between with/without the particles.


Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_34 | Pages 440 - 440
1 Dec 2013
Murakami T Sakai N Yamaguchi T Yarimitsu S Nakashima K Sawae Y Suzuki A
Full Access

In joint prostheses using ultra-high molecular weight polyethylene (UHMWPE) as bearing material, wear problems are not yet completely solved under severe conditions in various daily activities, although efficacious treatments such as crosslinking, addition of vitamin E and the grafting of phospholipid polymer improved the wear properties. In contrast, in healthy natural synovial joints possessing articular cartilage as biphasic bearing material lubricated with synovial fluid, minimal wear with extremely low friction has been maintained for a whole life. Therefore, the joint prosthesis with artificial hydrogel cartilage with similar properties to articular cartilage is expected to show superior tribological functions with very low friction and infinitesimal wear if the appropriate lubrication mechanism is actualized. In this study, the effectiveness of biphasic lubrication mechanism in hydrogel through significant load support by fluid phase is evaluated in finite element (FE) analysis for reciprocating motion.

As biocompatible artificial hydrogel cartilage materials, two kinds of poly (vinyl alcohol) (PVA) hydrogels were prepared by the repeated freezing-thawing method and the cast-drying method, which are physically crosslinked with hydrogen bonding but different in structure and mechanical properties. To evaluate these time dependent behaviors of load-support ratio of fluid/solid phases and friction, two-dimensional biphasic FE analysis for cylindrical PVA hydrogel cartilage as 1.5 mm thick soft layer and radius of 5 mm was conducted under continuous loading of 0.2 N/mm by impermeable rigid plate in reciprocating motion in Fig. 1. The sliding speed is 4 mm/s for stroke of 8 mm at period of 4 s. A commercial package ABAQUS (6.8–4), which was appropriately evaluated for the biphasic FE analyses, was used in this study. The biphasic tissue was modeled by CPE4RP (four-node bilinear displacement and pore pressure, reduced integration with hour glass control) elements. The mechanical properties such as permeability, Young's modulus and Poisson ratio were estimated by curve fitting to stress relaxation behaviors in compression test.

As indicated in Fig. 2, it is worth noting that the cast-drying PVA shows significant interstitial fluid pressurization compared with a repeated freezing-thawing PVA hydrogel at 292 s after start-up, where coefficient of friction for solid-to-solid was assumed as 0.2. Changes in friction for PVA hydrogels in reciprocating motion were estimated as shown in Fig. 3. In spite of high friction (0.2) for solid-to-solid, cast-drying PVA brought the gradual decreasing in friction, probably due to rising of load-support ratio by fluid phase from initial 74% to 80%.

In human body, lubricating constituents in synovial fluids such as hyaluronic acid, proteins, glycoproteins and phospholipids can reduce the coefficient of friction for solid-to-solid. As suggested for low coefficient of friction for solid-to-solid as 0.01 in Fig. 3, rubbing friction is expected to be reduced to significantly low level.

As described above, the effective biphasic lubrication can sustain low friction level and minimal wear in synergistic action with soft-elastohydrodynamic lubrication, hydration lubrication and boundary lubrication as a similar mechanism to natural cartilage in various daily activities. These results indicate the usefulness of artificial hydrogel cartilage for longer durability in joint prostheses for clinical application.


Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_15 | Pages 271 - 271
1 Mar 2013
Murakami T Yarimitsu S Nakashima K Sawae Y Sakai N Araki T Suzuki A
Full Access

Various treatments for ultra-high molecular polyethylene (UHMWPE) such as cross-linking, addition of vitamin E and the grafting of phospholipid polymer improved the wear properties. However, wear problems still occur in joint prostheses in mixed or boundary lubrication modes under severe conditions. As an alternate method, the joint prosthesis with artificial hydrogel cartilage with similar properties to articular cartilage is expected to show superior tribological functions with very low friction and low wear if the adaptive multimode lubrication mechanism is actualized. In this study, the effectiveness of hydrogel structure and adsorbed film formed on artificial cartilage surfaces is examined in reciprocating tests in related to biphasic, hydration and boundary lubrication modes.

The frictional behaviors of artificial cartilage materials against flat glass plate in the reciprocating test were observed. As upper specimens, poly(vinyl alcohol) (PVA) hydrogel ellipsoidal specimen as 2 mm soft layer were prepared. PVA hydrogel specimens were prepared by the repeated freezing-thawing method and the cast-drying method. The sliding speed and stroke length were 20 mm/s and 35 mm, respectively. Applied load was 2.94 N or 9.8 N. The lubricants are saline or saline solutions containing L-α-Dipalmitoyl phosphatidyl-choline (DPPC), serum protein and/or hyaluronate(HA).

As shown in Fig. 1, the repeated freezing-thawing PVA shows gradual increase in friction from initial medium value immediately after loading of 2.94 N to high level. For the same test condition, the articular cartilage exhibited similar time-dependent frictional behavior from initial lower friction to high level as estimated by biphasic lubrication theory. On the contrary, it is noticed that a low friction is maintained for cast-drying PVA hydrogel, particularly two-layer laminated PVA hydrogel until 140 m sliding. The improvement of frictional behaviors in cast-drying PVA hydrogel is considered to have been brought about by the improvement of water retention ability of the hydrogel with uniform microstructure controlled by hydrogen bond.

Next, the influence of lubricant constituents on tribological behaviors of freezing-thawing PVA hydrogel was examined in repeated reciprocating test including unloading-restarting process at each 36 m sliding at 9.8 N. The frictional behavior for the freezing-thawing PVA hydrogel could be improved with supplying appropriate lubricant constituents as shown in Fig. 2. In lubricated condition with HA solution containing 0.01 wt% DPPC, 1.4 wt% albumin and 0.7 wt% γ-globulin, low friction was maintained and very little visible wear was confirmed in micrograph. Adsorbed films appear to contribute to the effective synergistic lubrication even under high load of 9.8 N in reciprocating test.

As described above, the effectiveness of synergistic lubrication for PVA hydrogel specimens is shown for improvement of tribological behaviors of artificial cartilage as a superior mechanism to natural cartilage. These results indicate the possibility of artificial hydrogel cartilage for longer durability in joint prostheses for clinical application.