Abstract. Objectives. Three-dimensional visualisation of sonographic callus has the potential to improve the accuracy and accessibility of ultrasound evaluation of fracture healing. The aim of this study was to establish a reliable method for producing three-dimensional reconstruction of sonographic callus. Methods. A prospective cohort of ten patients with a closed tibial shaft fracture managed with intramedullary nailing were recruited and underwent ultrasound scanning at 2-, 6- and 12-weeks post-surgery. Ultrasound B-mode capture was performed using infrared tracking technology to map each image to a three-dimensional lattice. Using echo intensity, semi-automated mapping was performed by two independent reviewers to produce an anatomic three-dimensional representation of the fracture. Agreement on the presence of sonographic bridging callus on three-dimensional reconstructions was assessed using the kappa coefficient. Results. Nine of the ten patients achieved union at six months. At six weeks, seven patients had bridging callus at ≥1 cortex on the three-dimensional reconstruction; when present all united. Compared to radiographs, no bridging callus was present in any patient. Of the three patients lacking sonographic bridging callus, one went onto a nonunion (77.8%-sensitive and 100%-specific to predict union). At twelve weeks, nine patients had bridging callus at ≥1 cortex on three-dimensional reconstruction and all united (100%-sensitive and 100%-specific to predict union). Compared to radiographs, seven of the nine patients that united had bridging callus. Three-dimensional reconstruction of the anteromedial and anterolateral tibial surface was achieved in all patients, and detection of sonographic bridging callus on the three-dimensional reconstruction demonstrated substantial inter-observer agreement (kappa=0.78, 95% confidence interval 0.29–1.0, p=0.011). Conclusions. Three-dimensional fracture reconstruction can be created using multiple ultrasound images in order to evaluate the presence of bridging callus. This imaging modality has the potential to identify impaired healing at an early stage in fracture management. Declaration of Interest. (b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project

Three-dimensional (3D) printing has become more frequently used in surgical specialties in recent years. Orthopaedic surgery is particularly well-suited to 3D printing applications, and thus has seen a variety of uses for this technology. These uses include pre-operative planning, patient-specific instrumentation (PSI), and patient-specific implant production. As with any new technology, it is important to assess the clinical impact, if any, of three-dimensional printing. The purpose of this review was to answer the following questions: . What are the current clinical uses of 3D printing in orthopaedic surgery?. Does the use of 3D printing have an effect on peri-operative outcomes?. Four electronic databases (Embase, MEDLINE, PubMed, Web of Science) were searched for Articles discussing clinical applications of 3D printing in orthopaedics up to November 13, 2018. Titles, abstracts, and full texts were screened in duplicate and data was abstracted. Descriptive analysis was performed for all studies. A meta-analysis was performed among eligible studies to compare estimated blood loss (EBL), operative time, and fluoroscopy use between 3D printing cases and controls. Study quality was assessed using the Methodological Index for Non-Randomized Studies (MINORS) criteria for non-randomized studies and the Cochrane Risk of Bias Tool for randomized controlled trials (RCTs). This review was prospectively registered on PROSPERO (Registration ID: CRD42018099144). One-hundred and eight studies were included, published between 2012 and 2018. A total of 2328 patients were included in these studies, and 1558 patients were treated using 3D printing technology. The mean age of patients, where reported, was 47 years old (range 3 to 90). Three-dimensional printing was most commonly reported in trauma (N = 41) and oncology (N = 22). Pre-operative planning was the most common use of 3D printing (N = 63), followed by final implants (N = 32) and PSI (N = 22). Titanium was the most commonly used 3D printing material (16 studies, 27.1%). A wide range of costs were reported for 3D printing applications, ranging from “less than $10” to $20,000. The mean MINORS score for non-randomized studies was 8.3/16 for non-comparative studies (N = 78), and 17.7/24 for non-randomized comparative studies (N = 19). Among RCTs, the most commonly identified sources of bias were for performance and detection biases. Three-dimensional printing resulted in a statistically significant decrease in mean operative time (−15.6 mins, p < .00001), mean EBL (−35.9 mL, p<.00001), and mean fluoroscopy shots (−3.5 shots, p < .00001) in 3D printing patients compared to controls. The uses of 3D printing in orthopaedic surgery are growing rapidly, with its use being most common in trauma and oncology. Pre-operative planning is the most common use of 3D printing in orthopaedics. The use of 3D printing significantly reduces EBL, operative time, and fluoroscopy use compared to controls. Future research is needed to confirm and clarify the magnitude of these effects

Background. There is no consensus on which glenoid plane should be used in total shoulder arthroplasty. Nevertheless, anatomical reconstruction of this plane is imperative for the success of a total shoulder arthroplasty. Methods. Three-dimensional reconstruction CT-scans were performed on 152 healthy shoulders. Four different glenoid planes, each determined by three surgical accessible bony reference points, are determined. The first two are triangular planes, defined by the most anterior and posterior point of the glenoid and respectively the most inferior point for the Saller's Inferior plane and the most superior point for the Saller's Superior plane. The third plane is formed by the best fitting circle of the superior tubercle and the most anterior and posterior point at the distal third of the glenoid (Circular Max). The fourth plane is formed by the best fitting circle of three points at the rim of the inferior quadrants of the glenoid (Circular Inferior). We hypothesized that the plane with normally distributed parameters, narrowest variability and best reproducibility would be the most suitable surgical glenoid plane. Results. No difference in position of the mean humeral center of rotation is found between the Circular Max and Circular Inferior plane (X=91.71degrees/X=91.66degrees p=0.907 and Y=90.83degrees /Y=91.7degrees p=0.054 respectively), while clear deviations are found for the Saller's Inferior and Saller's Superior plane (p < 0.001). The Circular Inferior plane has the lowest variability to the coronal scapular plane (p<0.001). Conclusion. This study provides arguments to use the Circular Inferior glenoid plane as preferred surgical plane of the glenoid. Level of evidence: Level II, Basic Science Study, Anatomical Survey

Sonographic callus may enable assessment of fracture healing. The aim of this study was to establish a reliable method for three-dimensional reconstruction of sonographic callus. Patients that underwent non-operative management of displaced midshaft clavicle fractures and intramedullary nailing of tibia fractures were prospectively recruited and followed to union. Ultrasound scanning was performed at periodical time points following injury. Infra-red tracking technology was used to map each image to a three-dimensional lattice. Criteria was fist established for two-dimensional bridging callus detection in a pilot study. Using echo intensity of the ultrasound image, semi-automated mapping was used to create an anatomic three-dimensional representation of fracture healing. Agreement on the presence of sonographic bridging callus was assessed using the kappa coefficient and intra-class-correlation (ICC) between observers. 112 clavicle fractures and 10 tibia fractures completed follow-up at six months. Sonographic bridging callus was detected in 62.5% (n=70/112) of the clavicles at six weeks post-injury. If present, union occurred in 98.6% of the fractures (n=69/70). If absent, nonunion developed in 40.5% of cases (n=17/42)(73.4%-sensitive and 100%-specific to predict union). Out of 10 tibia fractures, 7 had bridging callus of at least one cortex at 6 weeks and when present all united. Of the three patients lacking sonographic bridging callus, one went onto a nonunion (77.8%-sensitive and 100%-specific to predict union). The ICC for sonographic callus between four reviewers was 0.82 (95% CI 0.68–0.91). Three-dimensional ultrasound reconstruction of bridging callus has the potential to identify impaired fracture healing at an early stage in fracture management

Total temporomandibular joint (TMJ) replacements reduce pain and improve quality of life in patients suffering from end-stage TMJ disorders, such as osteoarthritis and trauma. Jaw kinematics measurements following TMJ arthroplasty provide a basis for evaluating implant performance and jaw function. The aim of this study is to provide the first measurements of three-dimensional kinematics of the jaw in patients following unilateral and bilateral prosthetic TMJ surgeries. Jaw motion tracking experiments were performed on 7 healthy control participants, 3 unilateral and 1 bilateral TMJ replacement patients. Custom-made mouthpieces were manufactured for each participant's mandibular and maxillary teeth, with each supporting three retroreflective markers anterior to the participant's lip line. Participants performed 15 trials each of maximum jaw opening, lateral and protrusive movements. Marker trajectories were simultaneously measured using an optoelectronic tracking system. Laser scans taken of each dental plate, together with CT scans of each patient, were used to register the plate position to each participant's jaw geometry, allowing 3D condylar motion to be quantified from the marker trajectories. The maximum mouth opening capacity of joint replacement patients was comparable to healthy controls with average incisal inferior translations of 37.5mm, 38.4mm and 33.6mm for the controls, unilateral and bilateral joint replacement patients respectively. During mouth opening the maximum anterior translation of prosthetic condyles was 2.4mm, compared to 10.6mm for controls. Prosthetic condyles had limited anterior motion compared to natural condyles, in unilateral patients this resulted in asymmetric opening and protrusive movements and the capacity to laterally move their jaw towards their pathological side only. For the bilateral patient, protrusive and lateral jaw movement capacity was minimal. Total TMJ replacement surgery facilitates normal mouth opening capacity and lateral and inferior condylar movements but limits anterior condylar motion. This study provides future direction for TMJ implant design

Abstract. OBJECTIVES. An unresolved challenge in osteoarthritis research is characterising the localised intra-tissue mechanical response of articular cartilage. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) and digital volume correlation (DVC) permit non-destructive visualisation of three-dimensional (3D) strain fields in human articular cartilage. METHODS. Human articular cartilage specimens were harvested from the knee (n=4 specimens from 2 doners), mounted into a loading device and imaged in the loaded and unloaded state using a micro-CT scanner. Strain was calculated throughout the volume of the cartilage using the CT image data. RESULTS. Strain was calculated in the 3D volume with a spatial resolution of 75 µm, and the volumetric DVC calculated strain was within 5% of the known applied stain. Variation in strain distribution between the superficial, middle and deep zones was observed, consistent with the different architecture of the material in these locations. CONCLUSIONS. The DVC method is suitable for calculating strain in human articular cartilage. This method will be useful to generate chondral repair scaffolds that that seek to replicate the strain gradient in cartilage. Declaration of Interest. (b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project

Background. The femoral head center shift on reduction time in total hip arthroplasty (THA) causes alteration of the muscle tension around the hip joint. Many studies about the shift of the femoral head in the cranio-caudal direction or medio-lateral direction on coronal plane have been reported. It has been known widely that the shift on these directions influence tension of the abductor muscle around the hip joint. Nevertheless few studies about the three-dimensional shift including the antero-posterior direction have been reported. Purpose. The purpose of this study is to evaluate the three-dimensional shift of the femoral head center in THA using three-dimensional THA templating software. Subjects & Methods. The subjects of this study were 156 primary THA cases of 143 patients. Using CT-based three-dimensional THA templating software ZedHip® (LEXI, Tokyo Japan), simulation of optimal implantation was performed on each THA case. On case which has over anteverted or less anteverted femoral neck, a stem which has modular neck system was selected to adjust anteversion of the femoral neck. The three-dimensional shift of the femoral head center on reduction time was calculated with ZedHip®. The three-dimensional shift was resolve into cranio-caudal, medio-lateral and antero-posterior direction (Fig. 1). Furthermore the correlation between the amount of the shift and hip joint deformity was investigated. Results. The average amount of the shift on cranio-caudal direction was 9.9mm to caudal side, on medio-lateral direction was 3.1mm to medial side and on antero-posterior direction was 2.6mm to posterior side. The average total amount of three-dimensional shift was 12.9mm (Fig. 2). On Crowe type 1 hips in 88 cases, the average shift to posterior side was 3.2mm, on Crowe type 2 in 20 cases was 3.7mm and on Crowe type 3 in 13 cases was 4.0mm. Among them there was no significant difference (Fig. 3). Conclusion. At THA surgery, the femoral head center shifted three-dimensionally and the maximum amount of shift on antero-posterior direction was 16.6mm to posterior side. There was no correlation between these amounts of the shift on antero-posterior direction and anatomical deformity of the hip joint. It is important to understand the shift of the femoral head center for predicting the alteration of muscle tension around the hip joint. The shift on antero-posterior direction influences the tension of iliopsoas muscle and there is a possibility that the shift to posterior side causes anterior iliopsoas impingement after THA surgery

Objective. In total knee arthroplasty, three-dimensional “criss-cross” line locate femoral osteotomy and conventional osteotomy were used. By comparing the two methods osteotomy in patients before and after surgery and imaging-related information data, to evaluate the recent post operative efficacy, at the same time to find out if there has clinical evidence that three-dimensional “criss-cross” line locate femoral osteotomy can be used in total knee arthroplasty. Methods. From July 2012 to July 2014, 64 patients who undertook the artificial total knee arthroplasty were divided into 2 groups: conventional osteotomy group(group A)and three-dimensional “criss-cross” line locate femoral osteotomy group(group B). In the X-ray of the two groups, it was measured that the hip-knee-ankle angle and the joint gap symmetry of 90°flexion degree. It was also measured that the two group joints range of motion. Those data were statistically analyzed. The KSS score of the two groups were compared. Results. In Group B the excellent and good rate was 93.8%, and Group A was 81.3%. The postoperative results of Group A were relatively better than Group B in limb alignment and joint mobility. There were significant differences between the prosthesis placement of the two group patients. Conclusion. The Short-term results of the three-dimensional “criss-cross” line locate femoral osteotomy group was better than the conventional osteotomy group. The reference osteotomy method of three-dimensional “criss-cross” line is very helpful to have a good result in TKA

We have measured the three-dimensional patterns of carpal deformity in 20 wrists in 20 rheumatoid patients in which the carpal bones were shifted ulnarwards on plain radiography. Three-dimensional bone models of the carpus and radius were created by computerised tomography with the wrist in the neutral position. The location of the centroids and rotational angle of each carpal bone relative to the radius were calculated and compared with those of ten normal wrists. In the radiocarpal joint, the proximal row was flexed and the centroids of all carpal bones translocated in an ulnar, proximal and volar direction with loss of congruity. In the midcarpal joint, the distal row was extended and congruity generally well preserved. These findings may facilitate more positive use of radiocarpal fusion alone for the deformed rheumatoid wrist

We used three-dimensional movement analysis by computer modelling of knee flexion from 0° to 50° in 14 knees in 12 patients with recurrent patellar dislocation and in 15 knees in ten normal control subjects to compare the in vivo three-dimensional movement of the patella. Flexion, tilt and spin of the patella were described in terms of rotation angles from 0°. The location of the patella and the tibial tubercle were evaluated using parameters expressed as percentage patellar shift and percentage tubercle shift. Patellar inclination to the femur was also measured and patellofemoral contact was qualitatively and quantitatively analysed. The patients had greater values of spin from 20° to 50°, while there were no statistically significant differences in flexion and tilt. The patients also had greater percentage patellar shift from 0° to 50°, percentage tubercle shift at 0° and 10° and patellar inclination from 0° to 50° with a smaller oval-shaped contact area from 20° to 50° moving downwards on the lateral facet. Patellar movement analysis using a three-dimensional computer model is useful to clearly demonstrate differences between patients with recurrent dislocation of the patella and normal control subjects

Using roentgen stereophotogrammetry we have recorded the three-dimensional movements of the knee during an anteroposterior laxity test in 36 patients with torn anterior cruciate ligaments and in three cadaver knees. At 30 degrees of knee flexion and before loads were applied the tibia occupied a more laterally rotated position if the anterior cruciate ligament had been injured. When the tibia was pulled anteriorly knees with cruciate deficiency rotated more laterally and were more abducted than normal knees. Posterior traction induced lateral rotation in the injured knee and medial rotation in the intact one. Precise knowledge of the three-dimensional instability of the anterior cruciate deficient knee may be important when the laxity is evaluated only in relation to one of the three cardinal axes

This paper describes how advances in three-dimensional printing may benefit the military trauma patient, both deployed on operations and in the firm base. Use of rapid prototype manufacturing to produce a 3D representation of complex fractures that can be held and rotated will aid surgical planning within multidisciplinary teams. Patient-clinician interaction can also be aided using these graspable models. The education of military surgeons could improve with the subsequent accurate, inexpensive models for anatomy and surgical technique instruction. The developing sphere of additive manufacturing (3D printing functional end-use components) lends itself to further advantages for the military orthopaedic surgeon. Military trauma patients could benefit from advances in direct metal laser sintering which enable the manufacture of complex surfaces and porous structures on bio-metallic implants not possible using conventional manufacturing. “Bio-printing” of tissues mimicking anatomical structures has potential for military trauma patients with bone defects. Deployed surgeons operating on less familiar fracture sites could benefit from three-dimensionally printing patient-specific medical devices. These can make operating technically easier, reducing radiation exposure and operating time. Further ahead, it may be possible to contemporaneously 3D print medical devices unavailable from the logistics chain whilst operating in the deployed environment

We report the case of a 12-year-old boy with flexion loss in the left elbow caused by deficient of the concavity corresponding to the coronoid fossa in the distal humerus. The range of motion (ROM) was 15°/100°, and pain was induced by passive terminal flexion. Plain radiographs revealed complete epiphyseal closure, and computed tomography (CT) revealed a flat anterior surface of the distal humerus; the coronoid fossa was absent. Then, the bony morphometric contour was surgically recreated using a navigation system and a three-dimensional elbow joint model. A three-dimensional model of the elbow joint was made preoperatively and the model comprising the distal humerus was milled so that elbow flexion flexion of more than 140° could be achieved against the proximal ulna and radius. Navigation-assisted surgery (contouring arthroplasty) was performed using CT data from this milled three-dimensional model. Subsequently, an intraoperative passive elbow flexion of 135° was obtained. However, active elbow flexion was still inadequate one year after operation, and a triceps lengthening procedure was performed. At the final follow-up one year after triceps lengthening, a considerable improvement in flexion was observed with a ROM of −12°/125°. Plain radiographs revealed no signs of degenerative change, and CT revealed the formation of the radial and coronoid fossae on the anterior surface of the distal humerus. Navigation-assisted surgery for deformity of the distal humerus based on a contoured three-dimensional model is extremely effective as it facilitates evaluation of the bony morphometry of the distal humerus. It is particularly useful as an indicator for milling the actual bone when a model of the mirror image of the unaffected side cannot be applied to the affected side as observed in our case

Summary Statement. We used three-dimensional software to assess different anatomic variables in the femur. The canal of Femur twisted slightly below the lesser trochanter in cases with a larger angle of anteversion. Introduction. Accurate positioning of the joint prosthesis is essential for successful total hip arthroplasty (THA). To aid in tailoring of the prosthesis, we used three-dimensional software to assess different anatomic variables in the femur. Patients & Methods. We used CT imaging data of the unaffected normal side of the 25 patients (22 females, age range 30 to 81 years) who underwent THA in 2012 in our hospital. The femur was reconstructed from CT data and measured using three-dimensional modeling software (Mimics 16.0 Materialise, Leuven, Belgium). We measured ellipse fitting to the medullary canal in the axial plane of the femur at 20-mm intervals. The angle between the major axis of those ellipses and the axis of the femoral neck was measured and expressed as the canal rotation. The distance between the lesser trochanter and the center of the femoral head was measured along the Z axis. Results. The major axes of the ellipses direct to medial, front and medial side in the level of epiphysis, above isthmus and distal portion respectively in all cases. The maximum rotated level was above isthmus. The rotation angle in the proximal portion ranged from 36 to 84 degrees (mean, 60.6 degrees, SD ± 12.1). The rotation angle of the distal portion ranged from 71 to 95 degrees (mean, 86.1 degrees, SD ± 6.1). Discussion/Conclusion. The torsion of the canal varied more widely between individuals in the proximal portion than did the distal portion. In addition, the torsion of the proximal aspect, although more variable, was on average smaller when the angle of anteversion was large. Because the canal twisted slightly below the lesser trochanter in cases with a larger angle of anteversion, it is suggested that attention to the degree of anteversion of a flat prosthesis stem is warranted

Purpose: Scoliosis is a three-dimensional deformation of the spinal column. Modern surgical techniques have attempted to address this 3D component of the problem but pre- and postoperative measurements lack precision. A solution is stereoradiographic 3D reconstruction providing 1.1 mm precision for vertebral shape and 1.4° precision for axial rotation. Material and methods: Ten patients (seven adolescents and three adults) with idiopathic scoliosis (mean 56°, range 36°–78°) were treated with an in situ arching method. A calibrated teleradiogram (AP and lateral view) was obtained before and after surgery. The spinal columns were reconstructed by stereoradiography. Six rotation angles were measured in the three planes for each vertebra and each intervertebral space, taking into account the curvatures and their apical and junctional zones. Results: Preoperatively, for thoracic scoliosis, measurements were: mean vertebral axial rotation (VAR) measured at the apex = 20°; mean lateral axial rotation (LAR) of the junctional zones = 30°; mean intervertebral rotation (IVR) = 10°. Depending on the curvatures, in situ arching yielded a 52–60% correction of the VAR at the apex, and 78–79% correction of the junctional zones. VLR of the junctional zoenes was improved 58–74%. Intervertebral sagittal rotation (ISR) at the summit (kyphosis) was improved 5.5° on the average. Discussion: Unlike computed tomoraphy where scans are obtained in the supine position, three-dimensional reconstruction of the spinal column enables a precise analysis of the loaded spine. Improvement was significant in the frontal plane with 18.3° and 21.4° improvement of the VLR for the thoracic and thoracolumbar junctional zones respectively, compared with the rod rotation where the peroperative stereophotogram showed a 9.6° and 8.6° gain respectively. There was a real improvement in VAR, differing from the literature where the rotation of the rod appears to be less pronounced. Conclusion: Three-dimensional reconstruction of the spinal column enables a segmentary analysis of scoliosis deformations. In addition, by enabling a view of the spinal column in all directions, angle measurements can be made with precision allowing repeated measurements and comparisons. This technique demonstrated the efficacy of in situ arching in improving vertebral rotation

We evaluated the impact of stereo-visualisation of three-dimensional volume-rendering CT datasets on the inter- and intraobserver reliability assessed by kappa values on the AO/OTA and Neer classifications in the assessment of proximal humeral fractures. Four independent observers classified 40 fractures according to the AO/OTA and Neer classifications using plain radiographs, two-dimensional CT scans and with stereo-visualised three-dimensional volume-rendering reconstructions. Both classification systems showed moderate interobserver reliability with plain radiographs and two-dimensional CT scans. Three-dimensional volume-rendered CT scans improved the interobserver reliability of both systems to good. Intraobserver reliability was moderate for both classifications when assessed by plain radiographs. Stereo visualisation of three-dimensional volume rendering improved intraobserver reliability to good for the AO/OTA method and to excellent for the Neer classification. These data support our opinion that stereo visualisation of three-dimensional volume-rendering datasets is of value when analysing and classifying complex fractures of the proximal humerus

We have studied the three-dimensional geometry of the proximal humerus on human cadaver specimens using a digitised measuring device linked to a computer. Our findings demonstrated the variable shape of the proximal humerus as well as its variable dimensions. The articular surface, which is part of a sphere varies individually in its orientation as regards inclination and retroversion, and it has variable medial and posterior offsets. These variations cannot be accommodated by the designs of most contemporary humeral components. Although good clinical results can be achieved with current modular and non-modular components their relatively fixed geometry prevents truly anatomical restoration in many cases. To try to restore the original three-dimensional geometry of the proximal humerus, we have developed a new type of humeral component which is modular and adaptable to the individual anatomy. Such adaptability allows correct positioning of the prosthetic head in relation to an individual anatomical neck, after removal of the marginal osteophytes. The design of this third-generation prosthesis respects the four geometrical variations which have been demonstrated in the present study. These are inclination, retroversion, medial offset and posterior offset

We created virtual three-dimensional reconstruction models from computed tomography scans obtained from patients with acetabular fractures. Virtual cylindrical implants were placed intraosseously in the anterior column, the posterior column and across the dome of the acetabulum. The maximum diameter which was entirely contained within the bone was determined for each position of the screw. In the same model, the cross-sectional diameters of the columns were measured and compared to the maximum diameter of the corresponding virtual implant. We found that the mean maximum diameter of virtual implant accommodated by the anterior columns was 6.4 mm and that the smallest diameter of the columns was larger than the maximum diameter of the equivalent virtual implant. This study suggests that the size of the screw used for percutaneous fixation of acetabular fractures should not be based solely on the measurement of cross-sectional diameter and that virtual three-dimensional reconstructions might be useful in pre-operative planning

We investigated the three-dimensional morphological differences of the articular surface of the femoral trochlea in patients with recurrent dislocation of the patella and a normal control group using three-dimensional computer models. There were 12 patients (12 knees) and ten control subjects (ten knees). Three-dimensional computer models of the femur, including the articular cartilage, were created. Evaluation was performed on the shape of the articular surface, focused on its convexity, and the proximal and mediolateral distribution of the articular cartilage of the femoral trochlea. The extent of any convexity, and the proximal distribution of the articular cartilage, expressed as the height, were shown by the angles about the transepicondylar axis. The mediolateral distribution of the articular cartilage was assessed by the location of the medial and lateral borders of the articular cartilage. The mean extent of convexity was 24.9° . sd. 6.7° for patients and 11.9° . sd. 3.6° for the control group (p < 0.001). The mean height of the articular cartilage was 91.3° . sd. 8.3° for the patients and 83.3° . sd. 7.7° for the control group (p = 0.03), suggesting a wider convex trochlea in the patients with recurrent dislocation of the patella caused by the proximally-extended convex area. The lateral border of the articular cartilage of the trochlea in the patients was more laterally located than in the control group. Our findings therefore quantitatively demonstrated differences in the shape and distribution of the articular cartilage on the femoral trochlea between patients with dislocation of the patella and normal subjects

The three-dimensional architecture of bovine articular cartilage collagen and its relationship to split lines has been studied with scanning electron microscopy. In the middle and superficial zones, collagen was organised in a layered or leaf-like manner. The orientation was vertical in the intermediate zone, curving to become horizontal and parallel to the articular surface in the superficial zone. Each leaf consisted of a fine network of collagen fibrils. Adjacent leaves merged or were closely linked by bridging fibrils and were arranged according to the split-line pattern. The surface layer (lamina splendens) was morphologically distinct. Although ordered, the overall collagen structure was different in each plane (anisotropic) a property described in previous morphological and biophysical studies. As all components of the articular cartilage matrix interact closely, the three-dimensional organisation of collagen is important when considering cartilage function and the processes of cartilage growth, injury and repair

Introduction. Kinematically or anatomically aligned total knee arthroplasty (TKA) has been reported to provide improved clinical outcomes by replicating patient's original joint line [1][2]. It has been known that tibial (joint line) varus varies among patients, and the tibial varus would increase over progression of arthritis and bone remodeling. For those patients with significant deformity, the current tibial varus may significantly differ from its pre-diseased state. In this exploratory study, geometry and alignment of the tibial growth plate were measured with respect to tibial anatomical landmarks in order to better understand modes of tibial deformity and seek possible application in reconstructing pre-diseased joint alignment. Methods. CT scans of sixteen healthy Japanese knees (M6:F10, Age 31.9±13.9 years) were studied. Three-dimensional reconstruction models were created using Mimics 17 (Materialise, Leuven, Belgium). First, a mid-sagittal tibial reference plane, for comparing the varus/valgus orientation of the tibial plateau to that of the growth plate, was defined by the medial margin of the tibial tuberosity, origin of the PCL and center of the foot joint. The tibial plateau (or joint line plane) was determined from three points; dwell point of femur (aligned in extension) on lateral tibial articular surface, and two points at anterior and posterior rim of medial tibial articular surface sampled in the sagittal view and coinciding with dwell point of femur on medial tibia. Then, a three-dimensional model of the tibial growth plate was extracted using the Livewire function and mask editing tools in Mimics. To determine 3D orientation of the growth plate (GP), the vertical mass moment of inertia axis was calculated for the 3D model. The inertia axes were also determined for medial and lateral half of the GP (Figure 1). Results. Tibial plateau (TP) had 2.39±1.72 degrees of varus in coronal view and 11.12±3.90 degrees of posterior inclination in sagittal view. The shape of the GP is noticeably different between medial and lateral. The medial half tends to incline posteriorly towards medial, while the lateral half is twisted anteriorly. In coronal view, GP axis was in 1.27±1.49 degrees valgus to midsagittal plane. Normal axis of the TP was in varus to the GP axis by 3.66±1.79 degrees. The GP medial half was in 5.81±2.49 degrees valgus and 1.63±2.59 degrees anteriorly inclined with respect to the TP. The GP lateral half was in 11.65±2.07 degrees varus and 18.66±4.44 degrees anteriorly inclined relative to the TP. Discussion. The preliminary results from 16 healthy knees suggested that the tibial growth plate is aligned to midsagittal plane and tibial plateau in varus/valgus orientations with relatively small variations. More study samples will be required to validate usefulness of this method in surgical planning. Distinctive shape difference for medial and lateral half of the growth plate was also observed. Future study should also include diseased knees with various levels of deformities

Glenoid and humeral head bone defects have long been recognized as major determinants in recurrent shoulder instability as well as main predictors of outcomes after surgical stabilization. However, a universally accepted method to quantify them is not available yet. The purpose of the present study is to describe a new CT method to quantify bipolar bone defects volume on a virtually generated 3D model and to evaluate its reproducibility.

A cross-sectional observational study has been conducted. Forty CT scans of both shoulders were randomly selected from a series of exams previously acquired on patients affected by anterior shoulder instability. Inclusion criterion was unilateral anterior shoulder instability with at least one episode of dislocation. Exclusion criteria were: bilateral shoulder instability; posterior or multidirectional instability, previous fractures and/or surgery to both shoulders; congenital or acquired inflammatory, neurological, or degenerative diseases. For all patients, CT exams of both shoulders were acquired at the same time following a standardized imaging protocol. The CT data sets were analysed on a standard desktop PC using the software 3D Slicer. Computer-based reconstruction of the Hill-Sachs and glenoid bone defect were performed through Boolean subtraction of the affected side from the contralateral one, resulting in a virtually generated bone fragment accurately fitting the defect. The volume of the bone fragments was then calculated. All measurements were conducted by two fellowship-trained orthopaedic shoulder surgeons. Each measurement was performed twice by one observer to assess intra-observer reliability. Inter and intra-observer reliability were calculated. Intraclass Correlation Coefficients (ICC) were calculated using a two-way random effect model and evaluation of absolute agreement. Confidence intervals (CI) were calculated at 95% confidence level for reliability coefficients. Reliability values range from 0 (no agreement) to 1 (maximum agreement).

The study included 34 males and 6 females. Mean age (+ SD) of patients was 36.7 + 10.10 years (range: 25 – 73 years). A bipolar bone defect was observed in all cases. Reliability of humeral head bone fragment measurements showed excellent intra-observer agreement (ICC: 0.92, CI 95%: 0.85 – 0.96) and very good interobserver agreement (ICC: 0.89, CI 95%: 0.80 – 0.94). Similarly, glenoid bone loss measurement resulted in excellent intra-observer reliability (ICC: 0.92, CI 95%: 0.85 – 0.96) and very good inter-observer agreement (ICC: 0.84, CI 95%:0.72 – 0.91).

In conclusion, matching affected and intact contralateral humeral head and glenoid by reconstruction on a computer-based virtual model allows identification of bipolar bone defects and enables quantitative determination of bone loss.

The three-dimensional (3D) correction of glenoid erosion is critical to the long-term success of total shoulder replacement (TSR). In order to characterise the 3D morphology of eroded glenoid surfaces, we looked for a set of morphological parameters useful for TSR planning. We defined a scapular coordinates system based on non-eroded bony landmarks. The maximum glenoid version was measured and specified in 3D by its orientation angle. Medialisation was considered relative to the spino-glenoid notch. We analysed regular CT scans of 19 normal (N) and 86 osteoarthritic (OA) scapulae. When the maximum version of OA shoulders was higher than 10°, the orientation was not only posterior, but extended in postero-superior (35%), postero-inferior (6%) and anterior sectors (4%). The medialisation of the glenoid was higher in OA than normal shoulders. The orientation angle of maximum version appeared as a critical parameter to specify the glenoid shape in 3D. It will be very useful in planning the best position for the glenoid in TSR. Cite this article: Bone Joint J 2014;96-B:513–18

Introduction:. For 30 years, uncemented anatomic hip stems have been implanted with documented clinical results[1,2]. Their geometry can be linked back to the geometry of the PCA and ABG stems. Modifications to date include stem length, body geometry, material, and reduction in distal geometry. New tools have been developed allowing anatomical measurements and analysis of three-dimensional digital femora geometry through CT scans[3]. The purpose of this study is to analyze three-dimensional contact of various anatomic hip stem designs using this technique. Methods:. Six femora (57–87 yrs, 72–88 kg), were selected from a CT scan database (SOMA™) of 604 Caucasian bones. They were selected based on femoral anteversion (average +/−1.5 * std. dev.) with three measuring[4] 8–10° and three 31–33° of anteversion. The CT scans were segmented into cancellous/cortical bone and converted into CAD models in PRO/Engineer Wildfire (v.5). A/P views of the bones were scaled to a 120% magnification to allow three surgeons to surgically template and choose the stem size and location (maximizing fill (n = 1); restoring the head center (n = 2)) with two implant designs (1-Citation TMZF and 2-ABG II Monolithic, Stryker Orthopaedics, Mahwah). Measurements from templating were used to virtually implant CAD models of the implants into the bones (n = 36 bone/stem assemblies). The assemblies were imported into Geomagic Qualify 2012 for 3D deviation analysis comparing the coated region of the implant to the cortical-cancellous boundary. The analysis generated color map profiles based on the following categories: Contact (−2.0 to 0.5 mm), Conformity (0.5 to 2.0 mm), Proximity (2.0 to 5.0 mm), and Gap (5.0 to 12 mm) and the percent of the surface that was within each of these categories. These results were compared for patterns within and across the anatomic families. Results:. Similar patterns of fit were observed within and across both families. The same size implants were not always used together across both systems. The strongest commonality was found regarding the percentage of the implant adjacent to more than 5 mm of cancellous bone (Gap, shown in red in Figure 1b) and the pattern of contact on the medial curvature of the implants. On average 61% and 56% of the metaphyseal region of Implants 1 and 2, respectively, is adjacent to 5–12 mm of cancellous bone between the implant and cortical bone. Implants 1 and 2 also demonstrated 30% and 37% between 0.5 and 5 mm of cancellous bone to the cortical boundary. Contact (< .5 mm) was only achieved in areas where bone would have been removed through femoral preparation. When maximizing fill, it was found that the percent Gap was reduced and distributed between conformity and proximity. There was also less variability between both systems when the goal was to maximize fill, however there was no statistical difference given the sample size between both stems regardless of method. Discussion:. Proper load transfer is essential for positive bone remodeling for short/long term fixation. As anatomic stems load femurs circumferentially, it is important to note that common characteristics transfer load to bone potentially contributing to their success. Previously, technology has not permitted circumferential analysis of implant fit on a wide scale, reproducible basis

Introduction:. The widespread use of TKA promoted studies on kinematics after TKA, particularly of the femorotibial joint. Knee joint kinematics after TKA, including the range of motion (ROM) and the physical performance, are also influenced by the biomechanical properties of the patella. Surgeons sometimes report complications after TKA involvinganterior knee pain, patellofemoral impingement and instability. However, only few studies have focused specially on the patella. Because the patella bone is small and overlapped with the femoral component on scan images. In addition, the patellar component in TKA is made of x-ray–permeable ultra-high molecular weight polyethylene. It is impossible to radiographically determine the external contour of the patellar component precisely. No methods have been established to date to track the dynamic in vivo trajectory of the patella component. In this study, we analyzed the in vivo three-dimensional kinematics of the patellar component in TKA by applying our image matching method with image correlations. Methods:. A computed tomography (CT) and an x-ray flat panel detector system (FPD) were used. FPD-derived post-TKA x-ray images of the residual patellar bone were matched by computer simulation with the virtual simulation images created using pre-TKA CT data. For the anatomic location of the patellar component, the positions of the holes drilled for the patellar component pegs were used. This study included three patients with a mean age of 68 years (three females with right knee replacement) who had undergone TKA with the Quest Knee System and achieved a mean passive ROM of 0 to ≥ 130° after 6 or more month post-TKA. We investigated three-dimensional movements of the patellar component in six degrees of freedom (6 DOF) during squatting and kneeling. Furthermore, we simulated the three-dimensional movement of the patellar component, and we estimated and visualized the contact points between the patellar and femoral components on a three-dimensional model. Results:. Average root mean square errors of this technique with the patellar bone of a fresh-frozen pig complete knee joint have been confirmed as 0.2 mm for the translations and 0.2 degrees for the rotation. The 6 DOF analysis results showed that patellar dynamics were similar for all subjects on squatting and kneeling. For the patellar rotation during squatting, only 1 to 2 additional degrees were noted for all subjects. During kneeling, the patellar rotation noted adduction for all subjects. The patellar contact point on the femoral component gradually showed superior shift, increasing the distance with knee flexion during squatting and kneeling (Fig, 1. 2). Discussions and Conclusions:. In this study, no patellar shifts were detected in rotation or tilt during squatting, suggesting that the patellar component remained in the positions designed for early stages of flexion. And the patellar component shifted towards the lateral side during squatting. This finding suggests the idea that the patellar movement reflected the design of the Quest Knee system. This study demonstrated that the analytical method is useful for evaluating the pathologies and post-surgical conditions of the knee and other joints

The purpose of this study is to investigate the three-dimensional (3D) kinematics of normal knees in deep knee-bending motions like squatting and kneeling. Material & Methods: We investigated the in vivo kinematics of 4 Japanese healthy male volunteers (8 normal knees in squatting, 7 normal knees in kneeling). Each sequential motion was performed under fluoroscopic surveillance in the sagittal plane. Femorotibial motion was analyzed using 2D/3D registration technique, which uses computer-assisted design (CAD) models to reproduce the spatial position of the femur and tibia from single-view fluoroscopic images. We evaluated the femoral rotation relative to the tibia and anteroposterior (AP) translation of the femoral sulcus and lateral epicondyle on the plane perpendicular to the tibial mechanical axis. Student's t test was used to analyze differences in the absolute value of axial rotation and AP translation of the femoral sulcus and lateral epicondyle during squatting and kneeling. Values of P < 0.05 were considered statistically significant. During squatting, knees were gradually flexed from −2.8 ± 1.3° to 145.5 ± 5.1° on average. Knees were gradually flexed from 100.8 ± 3.9° to 155.6 ± 3.2° on average during kneeling. Femurs during squatting displayed sharp external rotation relative to the tibia from 0° to 30° of flexion and it reached 12.5 ± 3.3° on average. From 30° to 130° of flexion, the femoral external rotation showed gradually, and it reached 19.1 ± 7.3° on average. From 130° to 140° of flexion, it was observed additionally, and reached 22.4 ± 6.1° on average. All kneeling knees displayed femoral external rotation relative to the tibia sharply from 100° to 150° of flexion, and it reached 20.7 ± 7.5° on average. From 100° to 120° of flexion, the femoral external rotation during squatting was larger than that during kneeling significantly. From 120° to 140° of flexion, there was no significant difference between squatting and kneeling. The sulcus during squatting moved 4.1 ± 4.8 mm anterior from 0° to 60° of flexion. From 60° of flexion it moved 13.6 ± 13.4 mm posterior. The sulcus during kneeling was not indicated significant movement with the knee flexion. The lateral epicondyle during squatting moved 39.4 ± 7.7 mm posterior from 0° to 140° of flexion. The lateral epicondyle during kneeling moved 22.0 ± 5.4 mm posterior movement from 100° to 150° of flexion. In AP translation of the sulcus from 100° to 140° of flexion, there was no significant difference between squatting and kneeling. However in that of the lateral epicondyle, squatting groups moved posterior significantly. Even if they were same deep knee-bending, the kinematics were different because of the differences of daily motions. The results in this study demonstrated that in vivo kinematics of deep knee-bending were different between squatting and kneeling

Purpose. The purpose of this study was to assess the accuracy of three-dimensional camera technology when monitoring deformity correction by an Ilizarov frame and to compare it to manual measurements. Methods and Results. A model consisting of an Ilizarov frame built around an artificial tibia and fibula was used with retro-reflective markers placed on the frame and bones to allow for the positions of each to be detected by the camera system. Measurements made by the camera system were compared to measurements taken manually. In the assessment of frame lengthening, the camera system average error was 2% (SD 2%) compared to 7% (SD 6%) for manual measurement. In the assessment of bone lengthening, the camera system average error was 4% (SD 4%) compared to 34% (SD 8%) for manual measurement. The technology also demonstrated good accuracy in the measurement of angular deformity changes. Conclusion. The results of this study demonstrate that the measurement of deformity correction with three-dimensional infra-red camera technology is superior to manual measurements in a model of deformity correction. This method could replace or greatly reduce x-ray exposure in monitoring deformity correction post-operatively

During total knee replacement (TKR), knee surgical navigation systems (KSNS) report in real time relative motion data between the tibia and the femur from the patient under anaesthesia, in order to identify best possible locations for the corresponding prosthesis components. These systems are meant to support the surgeon for achieving the best possible replication of natural knee motion, compatible with the prosthesis design and the joint status, in the hope that this kinematics under passive condition will be then the same during the daily living activities of the patient. Particularly, by means of KSNS, knee kinematics is tracked in the original arthritic joint at the beginning of the operation, intra-operatively after adjustments of bone cuts and trial components implantation, and after final components implantation and cementation. Rarely the extent to which the kinematics in the latter condition is then replicated during activity is analysed. As for the assessment of the active motion performance, the most accurate technique for the in-vivo measurements of replaced joint kinematics is three-dimensional video-fluoroscopy. This allows joint motion tracking under typical movements and loads of daily living. The general aim of this study is assessing the capability of the current KSNS to predict replaced joint motion after TKR. Particularly, the specific objective is to compare, for a number of patients implanted with two different TKR prosthesis component designs, knee kinematics obtained intra-operatively after final component implantation measured by means of KSNS with that assessed post-operatively at the follow-up by means of three-dimensional video-fluoroscopy. Thirty-one patients affected by primary gonarthrosis were implanted with a fixed bearing posterior-stabilized TKR design, either the Journey® (JOU; Smith&Nephew, London, UK) or the NRG® (Stryker®-Orthopaedics, Mahwah, NJ-USA). All implantations were performed by means of a KSNS (Stryker®-Leibinger, Freiburg, Germany), utilised to track and store joint kinematics intra-operatively immediately after final component implantation (INTRA-OP). Six months after TKR, the patients were followed for clinical assessment and three-dimensional video fluoroscopy (POST-OP). Fifteen of these patients, 8 with the JOU and 7 with the NRG, gave informed consent and these were analyzed. At surgery (INTRA-OP), a spatial tracker of the navigation system was attached through two bi-cortical 3 mm thick Kirschner wires to the distal femur and another to the proximal tibia. The conventional navigation procedure recommended in the system manual was performed to calculate the preoperative deformity including the preoperative lower limb alignment, to perform the femoral and tibial bone cuts, and to measure the final lower limb alignment. All these assessment were calculated with respect to the initial anatomical survey, the latter being based on calibrations of anatomical landmarks by an instrumented pointer. Patients were then analysed (POST-OP) by three-dimensional video-fluoroscopy (digital remote-controlled diagnostic Alpha90SX16; CAT Medical System, Rome-Italy) at 10 frames per second during chair rising-sitting, stair climbing, and step up-down. A technique based on CAD-model shape matching was utilised for obtaining three-dimensional pose of the prosthesis components. Between the two techniques, the kinematics variables analysed for the comparison were the three components of the joint rotation (being the relative motion between the tibial and femoral components represented using a standard joint convention, the translation of the line through the medial and lateral contact points (being these points assumed to be where the minimum distance between the femoral condyles and the tibial baseplate is observed) on the tibial baseplate and the corresponding pivot point, and the location of the instantaneous helical axes with the corresponding mean helical axis and pivot point. In all patients and in both conditions, physiological ranges of flexion (from −5° to 120°), and ab-adduction (±5°) were observed. Internal-external rotation patterns are different between the two prostheses, with a more central pivoting in NRG and medial pivoting in JOU, as expected by the design. Restoration of knee joint normal kinematics was demonstrated also by the coupling of the internal rotation with flexion, as well as by the roll-back and screw-home mechanisms, observed somehow both in INTRA- and POST-OP measurements. Location of the mean helical axis and pivot point, both from the contact lines and helical axes, were very consistent over time, i.e. after six months from intervention and in fully different conditions. Only one JOU and one NRG patient had the pivot point location POST-OP different from that INTRA-OP, despite cases of paradoxical translation. In all TKR knees analysed, a good restoration of normal joint motion was observed, both during operation and at the follow-up. This supports the general efficacy of the surgery and of both prosthesis designs. Particularly, the results here reported show a good consistency of the measurements over time, no matter these were taken in very different joint conditions and by means of very different techniques. Intra-operative kinematics therefore does matter, and must be taken into careful consideration for the implantation of the prosthesis components. Joint kinematics should be tracked accurately during TKR surgery, and for this purpose KSNS seem to offer a very good support. These systems not only supports in real time the best possible alignment of the prosthesis components, but also make a reliable prediction of the motion performance of the replaced joint. Additional analyses will be necessary to support this with a statistical power, and to identify the most predicting parameters among the many kinematics variables here analysed preliminarily

Introduction and Aims: There is a relationship between the anatomy of the hip joint and the development of arthritis. A common cause of hip pain in the young adult that can lead to arthritis is acetabular dysplasia. More recently, femoroacetabular impingement has been described as another cause of hip pain. The purpose of our study was to evaluate the applicability of pelvic computed tomography (CT) with three-dimensional surface rendering to evaluate femoro-acetabular impingement. Method Thirty-six hips (30 patients; 17 males; 13 females) with persistent hip pain, mean age 41 (37–52), underwent three-dimensional CT of the pelvis, as well as MRI arthrography with gadolinium enhancement. On 3D CT, the concavity of the femoral head-neck junction (offset), alpha angle as described by Notzli was calculated to depict the anterior femoral neck contour. The concavity of the posterior aspect of the head neck junction was measured as the beta angle. The same measurements were made in 20 hips, consisting of randomly selected patients with no prior history of hip pathology or pain (mean age 37; 13 males; eight females). Results The mean alpha angle for the symptomatic group was 66.4 (39–94) and 43.8 (39.3–48.3) for the control group (p=0.001). All symptomatic hips had abnormal findings on MRA: labral tears in all; cartilage delamination/ulceration in 14 hips; herniation pits in six hips. The majority of labral tears and delamination were located in the antero-superior quadrant. In the surgical treated group, all MRA findings were confirmed. The mean beta angle was significantly smaller (increase concavity) in the symptomatic versus the controls: 40.2 versus 43.8 (p=0.011). Interestingly in the symptomatic group the beta angle was significantly lower than the alpha angle (p< 0.02), but not in the controls. Conclusion: 3D CT with surface rendering and multiplanar reformation is useful to determine degree of bone buttressing of the anterior femoral head-neck junction quantitatively assessed by alpha angle measurement, which is elevated in patients with femoro-acetabular impingement. With a greater posterior concavity i.e. small beta angle in the symptomatic group versus the control, subclinical slipped femoral epiphysis remains a plausible cause of this deformity

Objectives. Whilst gait speed is variable between healthy and injured adults, the extent to which speed alone alters the 3D in vivo knee kinematics has not been fully described. The purpose of this prospective study was to understand better the spatiotemporal and 3D knee kinematic changes induced by slow compared with normal self-selected walking speeds within young healthy adults. Methods. A total of 26 men and 25 women (18 to 35 years old) participated in this study. Participants walked on a treadmill with the KneeKG system at a slow imposed speed (2 km/hr) for three trials, then at a self-selected comfortable walking speed for another three trials. Paired t-tests, Wilcoxon signed-rank tests, Mann-Whitney U tests and Spearman’s rank correlation coefficients were conducted using Stata/IC 14 to compare kinematics of slow versus self-selected walking speed. Results. Both cadence and step length were reduced during slow gait compared with normal gait. Slow walking reduced flexion during standing (10.6° compared with 13.7°; p < 0.0001), and flexion range of movement (ROM) (53.1° compared with 57.3°; p < 0.0001). Slow walking also induced less adduction ROM (8.3° compared with 10.0°; p < 0.0001), rotation ROM (11.4. °. compared with 13.6. °. ; p < 0.0001), and anteroposterior translation ROM (8.5 mm compared with 10.1 mm; p < 0.0001). Conclusion. The reduced spatiotemporal measures, reduced flexion during stance, and knee ROM in all planes induced by slow walking demonstrate a stiff knee gait, similar to that previously demonstrated in osteoarthritis. Further research is required to determine if these characteristics induced in healthy knees by slow walking provide a valid model of osteoarthritic gait. Cite this article: N. Mannering, T. Young, T. Spelman, P. F. Choong. Three-dimensional knee kinematic analysis during treadmill gait: Slow imposed speed versus normal self-selected speed. Bone Joint Res 2017;6:514–521. DOI: 10.1302/2046-3758.68.BJR-2016-0296.R1

Introduction: Leg length and offset restoration are known to improve function after total hip arthroplasty, and to minimize the risk of dislocation and limp. Anatomic data of the hip are needed to determine specifications for prosthesis design that restore patient hip anatomy more closely. Furthermore, femoral off-set values calculated on X-Rays may be inaccurate in case of external rotational contracture or high femoral ante-version. The goal of this study was to determine three-dimensional morphological data of the hip in case of primary osteoarthritis, especially for femoral off-set. Material and Method: 223 hips with primary osteoarthritis have been analysed using a CT-scan and a specific software (HIP-PLAN. ®. ) that allows image post-processing for re-orienting the pelvis or the femur to a standardized orientation. Femoral and acetabular anteversions were measured. The planar (2D) and three-dimensional (3D) values of femoral offset were determined. 3D values were measured as the distance between the femoral head centre and the diaphyseal femur axis; 2D values were calculated as the projection of this distance on the frontal plan. Results: Measurements precision was good with correlation scores ranging between 0.91 and 0.99. Mean acetabular anteversion angle was 26° +/−6.6° when measured in the Anterior Pelvic Plane and 21.9° +/−6.6° in the frontal plane according to the method of Murray. Mean femoral anteversion was 21.9° +/−9.4 according to the method of Murphy. The Sum of acetabular and femoral anteversion was found to be out of the safe zone regarding dislocation risk in 47% of patients. Mean 3D femoral off-set was found to be 42.2 mm+/− 5, significantly increased by 3.5 mm +/− 2.5 when compared to the 2D femoral off-set values. Femoral off-set was above 45mm in 31% of cases and higher than 50 mm in 12% of cases. The tip of the great trochanter was located higher than the femoral head centre, at a mean distance of about 9 mm. Discussion: When measured on X-rays, femoral off-set may be significantly under-estimated. This error is probably due to the external rotational contracture of the hip induced by osteoarthritis. If the implants are positioned using the anatomical preoperative anteversion angles, 47% of patients would not be in the safe zone regarding posterior dislocation risk. Conclusions: Planar measurement using X-Rays underestimates significantly the femoral off-set. Neck and head modularity may be useful to achieve simultaneous restoration of femoral off-set and leg length in 12 to 31% of cases

This randomised study compared outcomes in patients with displaced fractures of the clavicle treated by open reduction and fixation by a reconstruction plate which was placed either superiorly or three-dimensionally. Between 2003 and 2006, 133 consecutive patients with a mean age of 44.2 years (18 to 60) with displaced midshaft fractures of the clavicle were allocated randomly to a three-dimensional (3D) (67 patients) or superior group (66). Outcome measures included the peri-operative outcome index, delayed union, revision surgery and symptoms beyond 16 weeks. CT was used to reconstruct an image of each affected clavicle and Photoshop 7.0 software employed to calculate the percentage of the clavicular cortical area in the sagittal plane. The patients were reviewed clinically and radiographically at four and 12 months after the operation. The superior plate group had a higher rate of delayed union and had more symptomatic patients than the 3D group (p < 0.05). The percentage comparisons of cortical bone area showed that cortical bone in the superior distal segment is thicker than in the inferior segment, it is also thicker in the anterior mid-section than in the posterior (p < 0.05). If fixation of midshaft fractures of the clavicle with a plate is indicated, a 3D reconstruction plate is better than one placed superiorly, because it is consistent with the stress distribution and shape of the clavicle

Although it is clear that opening-wedge high tibial osteotomy (HTO) changes alignment in the coronal plane, which is its objective, it is not clear how this procedure affects knee kinematics throughout the range of joint movement and in other planes. Our research question was: how does opening-wedge HTO change three-dimensional tibiofemoral and patellofemoral kinematics in loaded flexion in patients with varus deformity?Three-dimensional kinematics were assessed over 0° to 60° of loaded flexion using an MRI method before and after opening-wedge HTO in a cohort of 13 men (14 knees). Results obtained from an iterative statistical model found that at six and 12 months after operation, opening-wedge HTO caused increased anterior translation of the tibia (mean 2.6 mm, p <  0.001), decreased proximal translation of the patella (mean –2.2 mm, p <  0.001), decreased patellar spin (mean –1.4°, p < 0.05), increased patellar tilt (mean 2.2°, p < 0.05) and changed three other parameters. The mean Western Ontario and McMaster Universities Arthritis Index improved significantly (p < 0.001) from 49.6 (standard deviation (. sd. ) 16.4) pre-operatively to a mean of 28.2 (. sd. 16.6) at six months and a mean of 22.5 (. sd.  14.4) at 12 months. The three-dimensional kinematic changes found may be important in explaining inconsistency in clinical outcomes, and suggest that measures in addition to coronal plane alignment should be considered. . Cite this article: Bone Joint J 2014; 96-B:1214–21

Introduction: The purpose of this study was to evaluate the impact of volume rendering 3D computed tomography reconstructions on the inter- and intraobserver reliability of the OTA/AO and Neer classifications in the assessment of proximal humerus fractures. Material and Methods: Four observers with different levels of clinical training classified forty proximal humerus fractures according to the OTA/AO and Neer classifications. Three rounds of evaluation were performed and compared. First, fractures were classified on the basis of plain radiographs alone. Then, four weeks later, the combination of plain radiographs and computed tomography scans with conventional 3D SSD reconstructions was evaluated. Finally, four weeks later, the combination of plain radiographs, computed tomography scans, and 3D volume rendering reconstructions was assessed. These readings were repeated in a newly randomized order after an interval of twelve weeks to evaluate intraobserver reliability. Results: Interobserver reliability for the AO/ASIF classification showed good interobserver reliability with plain radiographs (k=0,65) and two-dimensional CT scans with conventional three-dimensional (SSD) reconstructions (k=0,71). Interobserver reliability improved to excellent when the fractures were classified on the basis of 3D volume rendering reconstructions scans (k=0,84). Intraobserver reliability of the OTA/AO classification was good with plain radiographs (k=0,70) and improved to excellent after adding three-dimensional SSD reconstructions (k=0,80) and three-dimensional VR reconstructions (k=0,88). Interobserver reliability of the Neer classification was poor with plain radiographs (k=0,39) and moderate with two-dimensional CT scans and conventional three-dimensional (SSD) reconstructions (k=0,56) and improved to good with the addition of 3D VR scans (k=0,74). Intraobserver reliability for was poor with plain radiographs (k=0,34), good with three-dimensional SSD reconstructions (k=0,61), and excellent with three-dimensional VR reconstructions (k=0,80). Conclusion: In this study, three-dimensional volume rendering computed tomography improved the inter- and intraobserver reliability of the AO/OTA and the Neer classifications in the assessment of proximal humerus fractures. In the opinion of the authors, 3D volume rendering CT-scans are a helpful tool for preoperative planning and classification of fractures of the proximal humerus

Introduction. According to the literature, the gait does not return to normal after THA. However, the three-dimensional hip anatomy is usually not assessed before and after surgery. Our hypothesis was that an accurate reconstruction of the hip anatomy, based on a three-dimensional preoperative planning, may normalize the gait after THA. Material and method. 18 consecutive patients, graded Charnley A, aged of 59.3 ±13 years, underwent THA for unilateral primary osteoarthritis using a direct anterior minimal invasive approach. A 3D computerised planning was performed, the implants size and position were chosen in order to restore, the leg length, the off-set, the centre of rotation and the anteversion angles (Figure 1). At 1 year follow up, a 3D gait analysis was performed and included 29 parameters describing the kinetics and the kinematics. Each patient was compared to himself using the contra-lateral healthy hip, as well as to a group of 13 healthy volunteers. Results. The real implants were the same than the ones planned in all the patients. The hip anatomy was restored with a high accuracy: 0.1±3mm for the hip rotation centre, −1.4±3 mm for the leg length and −0.9±3.5mm for the femoral offset. With respect to the gait, there was no significant difference between the operated side and the control-lateral leg. When compared to the control group, all the patients were within the normal range for all the parameters (Figure 2). Discussion and conclusion. The results suggest that the combination of an accurate 3D reconstruction and a direct anterior minimal invasive approach may allow to achieve a normal gait after THA at one year follow up

We evaluated the accuracy of a Magnetic Resonance Imaging (MRI)-based method to measure three-dimensional patellar tracking during loaded knee flexion. This method determines the relative positions of the knee bones by shape matching high-resolution three-dimensional geometric models of these bones to fast low-resolution scans taken during loaded flexion. The accuracy of the method’s assessment of patellar position and orientation was determined by comparing test measurements in four cadaver specimens to measurements made in the same specimens using Roentgen Stereophotogrammetric Analysis (RSA). This MRI-based method is more accurate than current two-dimensional imaging methods. The purpose of this study was to determine the accuracy of a MRI-based technique for measuring patellar tracking in loaded flexion. This novel, noninvasive, MRI-based method measures three-dimensional patellar tracking during loaded knee flexion with sufficient accuracy to detect clinically significant changes. Although abnormal patellar tracking is widely believed to be associated with pain and cartilage degeneration at the patella, these relationships have not been clearly established because most current methods assess only the two-dimensional alignment of the patella at one position. Measurements possible with this method should be sufficiently accurate to yield new insights into these relationships. Four cadaver knee specimens were flexed through seventy-five degrees of flexion in an MRI-compatible knee loading rig. A high-resolution image was acquired with each knee in extension and then a series of low-resolution scans (in two slice directions: axial and sagittal) were acquired through a flexion cycle. Segmenting bone outlines from high-resolution scans generated models of the femur, tibia and patella. These models were shape matched to the segmented bone outlines in the low resolution scans. Patellar attitude and position were determined and compared to measurements made using RSA. The mean measurement error in every kinematic parameter was lower for “fast” sagittal plane slices than for “fast” axial plane slices. In general, the mean measurement error was increased by decreasing the number of low-resolution slices. This method is more accurate than many two-dimensional methods, exposes participants to no ionizing radiation, and can be used through a large range of loaded knee flexion. Funding: Supported by an operating grant from the Canadian Institutes for Health Research and a Strategic Grant from the Natural Sciences and Engineering Research Council. NJM is supported by the Arthritis Society/CIHR Partnership Fund. Please contact author for figures and/or tables

Patellofemoral Pain Syndrome is characterized by anterior knee pain during activities such as squatting that is thought to be caused by abnormal patellar motion. However, the causative role has yet to be verified since it is difficult to measure the three-dimensional kinematics of the patellofemoral joint (PFJ) in vivo. We developed a fluoroscopy-based method to measure patellar motion as it moves under load through a cycle of flexion and compared the results with those obtained using Roentgen Stereophotogrammetric Analysis (RSA). Our data suggest that the fluoroscopy-based method has sufficient accuracy to detect clinically significant differences in patterns of patellar motion. The purpose of our study was to determine how accurately a fluoroscopy-based method measures patellar tracking. Our method measures three-dimensional PFJ kinematics with sufficient accuracy to be of clinical value in assessing dynamic motion. Patellar tracking can be assessed during aggravating activities to identify specific tracking abnormalities related to anterior knee pain. Four cadaver knees were imaged using computed tomography (CT). Surface models were generated and the coordinates of implanted tantalum beads (in the femur, patella, and tibia) were determined. A series of fluoroscopic images were taken with the knees loaded in a rig at various flexion angles. Each calibrated fluoroscopic image was registered to the CT model using a point-based method such that the high-resolution CT model was matched to the position of knee flexion associated with each fluoroscopic image. The patellar orientation and position relative to the femur was then reconstructed and described using a gyroscopic joint coordinate system. Measurements were made under the same test conditions using the established uniplanar RSA technique. Fluoroscopy-based and RSA-based measures of patellar orientation and position were compared. The mean measurement error (SD) for patellar flexion, spin, and tilt was 1.86 (1.55), 1.16 (1.14), and 1.15 (1.10) degrees, respectively. For proximal, lateral and anterior patellar translation, the mean measurement error (SD) was 2.11 (2.16), 0.59 (0.47), and 1.24 (1.18) mm, respectively. The accuracy of the fluoroscopy-based method of measuring PFJ kinematics was poorer than the reported accuracy of RSA but appears to be sufficiently low to be of clinical value. Funding: Supported by an operating grant from the Canadian Institutes for Health Research and a Strategic Grant from the Natural Sciences and Engineering Research Council. NJM is supported by TAS/CIHR Partnership Fund

Unknown femur orientation during X-ray imaging may cause inaccurate radiographic measurements. The aim of this study was to assess the effect of 3D femur orientation during radiographic imaging on the measurement of greater trochanter to femoral head centre (GT-FHC) distance. Three-dimensional femoral shapes (n=100) of unknown gender were generated using a statistical shape model based on a training data of 47 CT segmented femora. Rotations in the range of 0° internal to 50° external and 50° of flexion to 0° of extension (at 10 degree increments) were applied to each femur. A ray tracing algorithm was then used to create 2D images representing radiographs of the femora in known 3D orientations. The GT-FHC distance was then measured automatically by identifying the femoral head, shaft axis and tip of greater trochanter. Uniaxial rotations had little impact on the measurement with mean absolute error of 0.6 mm and 3.1 mm for 50° for pure external rotation and 50° pure flexion, respectively. Combined flexion and external rotation yielded more significant errors with 10° around each axis introducing a mean error of 3.6 mm and 20° showing an average error of 8.8 mm (Figure 1.). In the cohort we studied, when the femur was in neutral orientation, the tip of greater trochanter was never below the femoral head centre. Greater trochanter to femoral head centre measurement was insensitive to rotations around a single axis (i.e. flexion or external rotation). Modest combined rotations caused the tip of greater trochanter to appear more distal in 2D and led to deviation from the true value. This study suggests that a radiograph with the greater trochanter appearing below femoral head centre may have been acquired with 3D rotation of the femur. For any figures or tables, please contact the authors directly by clicking on ‘Info & Metrics’ above to access author contact details

A clinical and radiological study was conducted on 97 total hip replacements performed for congenital hip dislocation in 79 patients between 1989 and 1998 using a three-dimensional custom-made cementless stem. The mean age at operation was 48 years (17 to 72) and the mean follow-up was for 123 months (83 to 182). According to the Crowe classification, there were 37 class I, 28 class II, 13 class III and 19 class IV hips. The mean leg lengthening was 25 mm (5 to 58), the mean pre-operative femoral anteversion was 38.6° (2° to 86°) and the mean correction in the prosthetic neck was −23.6° (−71° to 13°). The mean Harris hip score improved from 58 (15 to 84) to 93 (40 to 100) points. A revision was required in six hips (6.2%). The overall survival rate was 89.5% (95% confidence interval 89.2 to 89.8) at 13 years when two hips were at risk. This custom-made cementless femoral component, which can be accommodated in the abnormal proximal femur and will correct the anteversion and frontal offset, provided good results without recourse to proximal femoral corrective osteotomy

Pre-operative computerised three-dimensional planning was carried out in 223 patients undergoing total hip replacement with a cementless acetabular component and a cementless modular-neck femoral stem. Components were chosen which best restored leg length and femoral offset. The post-operative restoration of the anatomy was assessed by CT and compared with the pre-operative plan. The component implanted was the same as that planned in 86% of the hips for the acetabular implant, 94% for the stem, and 93% for the neck-shaft angle. The rotational centre of the hip was restored with a mean accuracy of 0.73 mm (. sd. 3.5) craniocaudally and 1.2 mm (. sd. 2) laterally. Limb length was restored with a mean accuracy of 0.3 mm (. sd. 3.3) and femoral offset with a mean accuracy of 0.8 mm (. sd. 3.1). This method appears to offer high accuracy in hip reconstruction as the difficulties likely to be encountered when restoring the anatomy can be anticipated and solved pre-operatively by optimising the selection of implants. Modularity of the femoral neck helped to restore the femoral offset and limb length

Introduction: The goal of the study was to determine the precision of a three-dimensional pre-operative planning tool using a specific software (HIP-PLAN. ®. ) and an anatomic cementless neck-modular stem. Method: 223 patients who underwent a primary total hip replacement had a CT Scan before and after surgery. A pre-operative three-dimensional planning based on the CT-scan was performed. A cementless cup and a neck-modular stem were used. A computational matching of the pre-operative and the post-operative CT-scans was performed in order to compare the values of the planned anteversions and the planned displacement of the hip rotation center to the post-operative values. Results: The implanted component was the same as the one planned in 89% for the cup and 94% for the stem. For the mean femoral anteversion, there was no significant difference between the planned value (26.1° +/−11.8) and the post-operative value (26.9° +/−14.1). There was a poor correlation between the planned values and the actual ones for the acetabular cup anteversion (coefficient 0.17). The hip rotation center was restored with a precision of 0.73 mm +/3.5 horizontally and 1.2 mm +/−2 laterally. Limb length was restored with a precision of 0.3 mm +/−3.3 and the femoral off-set with a precision of 0.8 mm +/−3.1. There was no significant modification of the femoral off-set (0.07 p=0.7) which was restored or slightly increased in 93% of cases. Almost all the surgical difficulties were predicted. Conclusion: HIP-PLAN. ®. software is a reliable three-dimensional pre-operative planning tool which allows acurate prediction of components and hip anatomy

Introduction. Geometric variations of the hip joint can give rise to repeated abnormal contact between the femur and acetabular rim, resulting in cartilage and labrum damage. Population-based geometric parameterisation can facilitate the flexible and automated in silico generation of a range of clinically relevant hip geometries, allowing the position and size of cams to be defined precisely in three dimensions. This is advantageous compared to alpha angles, which are unreliable for stratifying populations by cam type. Alpha angles provide an indication of cam size in a single two-dimensional view, and high alpha angles have been observed in asymptomatic individuals. Parametric geometries can be developed into finite element models to assess the potential effects of morphological variations in bone on soft tissue strains. The aim of this study was to demonstrate the capabilities of our parameterisation research tool by assessing impingement severity resulting from a range of parametrically varied femoral and acetabular geometries. Methods. Custom made MATLAB (MathWorks) and Python codes. [1]. were used to generate bone surfaces, which were developed into finite element models in Abaqus (SIMULIA). Parametric femoral surfaces were defined by a spherical proximal head and ellipse sections through the neck/cam region. This method produced surfaces that were well fitted to bone geometry segmented from CT scans of cam patients and capable of producing trends in results similar to those found using segmented models. A simplified spherical geometry, including the labrum and acetabular cartilage, represented the acetabulum. Femoral parameters were adjusted to define relevant variations in cam size and position. Two radii (small and large cams) and two positions (anterior and superior cams) were defined resulting in four models. Alpha angles of these parametric femurs were measured in an anterior-posterior view and a cross-table lateral view using ImageJ (NIH). A further model was developed using a femur with a medium cam size and position, and the level of acetabular coverage and labrum length were varied. Bones were modelled as rigid bodies and soft tissues were modelled as transversely isotropic linearly elastic materials. With the acetabulum fully constrained in all cases, the femurs were constrained in translation and rotated to simulate flexion followed by internal rotation to cause impingement against the labrum. Results and Discussion. Models generated using the parametric approach showed that potential for tissue damage, indicated through local strain, was not predicted by measured alpha angle, but resulted from cam extent and position as defined by the ellipses. When variations were made to the acetabular rim, an increase in bone coverage had the greatest effect on impingement severity, indicated by strain in the cartilage labral-junction. An increase in labral length increased labral displacement, but had less effect on cartilage-labral strain. Patient specific models currently require full image segmentation, but there is potential to further develop these parametric methods to assess likely impingement severity based on a series of measures of the neck and acetabulum when three-dimensional imaging of patients is available

Objective. To three-dimensionally reconstruct the proximal femur of DDH (Developmental dysplasia of the hip) and measure the related anatomic parameters, so that we could have a further understanding of the morphological variation of the proximal femur of DDH, which would help in the preoperative planning and prosthesis design specific for DDH. Methods. From Jan.2012 to Dec.2014, 38 patients (47 hips) of DDH were admitted and 30 volunteers (30 hips) were selected as controls. All hips from both groups were examined by CT scan and radiographs. The Crowe classification method was applied. The CT data were imported into Mimics 17.0. The three-dimensional models of the proximal femur were then reconstructed, and the following parameters were measured: neck-shaft angle, neck length, offset, height of the centre of femoral head, height of the isthmus, height of greater trochanter, the medullary canal diameter of isthmus(Di), the medullary canal diameter 10mm above the apex of the lesser trochanter(DT+10), the medullary canal diameter 20mm below the apex of the lesser trochanter(DT-20), and then DT+10/Di, DT-20/Di and DT+10/DT-20 were calculated. Results. There is no significant difference in neck-shaft angle between Crowe I-III DDH and the control group, while the neck-shaft angle is much smaller in Crowe IV DDH. The neck length of Crowe IV DDH is much smaller than those of Crowe I-III DDH. As for Di there is neither significant difference between Crowe I DDH and the control group, nor significant difference between CroweII-III and Crowe IV, but the difference is significant between the first two groups and the latter two groups. DT+10/DT-20 and the offset have no significant difference between the control group and DDH groups. DT-20, DT+10, DT+10/Di and DT-20/Di are much smaller in Crowe IV DDH than that in Crowe I-III and the control groups. Height of greater trochanter in Crowe IV is larger than those in Crowe I-III and the control group. Height of the centre of femoral head in Crowe IV DDH is smaller than those in Crowe I-III DDH and the control group. The height of the isthmus in Crowe IV is much smaller than those in Crowe I-III DDH and the control group. Conclusion. The neck-shaft angle in DDH groups is not larger than that in the control group, while in contrast, it's much smaller in Crowe IV DDH than that in the control group. Comparing to Crowe I-III DDH and the control group, Crowe IV DDH has a dramatic change in the intramedullary and extramedullary parameters. The isthmus and the great trochanter are higher and there is apparent narrowing of the medullary canal around the level of the lesser trochanter

Minimally invasive (MIS) screw fixation for Hangman's fracture can decrease iatrogenic soft-tissue injury compared with conventional open approach, but increase the risk of instrumentation-related complications due to lack of anatomical landmarks. With the advantages, the intra-operative three-dimensional fluoroscopy-based navigation (ITFN) system seems to be an inherent partner for MIS techniques. The purpose of this study was to evaluate the accuracy and feasibility of MIS techniques incorporating with ITFN for treating Hangman's fracture. 20 patients with Hangman's fracture underwent C2-C3 pedicle screw fixation using ITFN. 6 patients used MIS technique, with the other 14 patients using conventional open technique. Preoperative visual analogue score (VAS) was 5.7±1.4 in CAOS-MIS group and 5.5±0.9 in CAOS-open group. Operative time, blood loss and postoperative neurovascular complications were recorded. The accuracy of screw positions was studied by postoperative CT scan. All patients were followed up for at least 6 months and the fusion status was ascertained by dynamic radiographs. The average operative time was 134.2±8.0 min in CAOS-MIS group and 139.3±25.8 min in CAOS-open group, and there was no significant difference between the two (p&gt;0.01). The blood loss was 66.7±25.8 ml in CAOS-MIS group and 250.0±141.4 ml in CAOS-open group. Statistical difference existed with CAOS-MIS group significant less than CAOS-open group (p&lt;0.01). A total of 80 screws were inserted. No screw-related neurovascular injury was observed. Post-operative CT scan revealed 83.3% (20/24) screws of grade 1 and 16.7% screws of grade 2 (4/24) in CAOS-MIS group, meanwhile 89.3% screws of grade 1 (50/56) and 10.7% screws of grade 2 (6/56) in CAOS-open group. There was no grade 3 screw detected. Fisher's exact test showed there was no statistical difference between these two groups (p&gt;0.01). There was no statistical difference in pre-operative VAS between these two groups (p&gt;0.01). Compared with the CAOS-open group (1.7±0.6), neck pain VAS at 6-month follow-up in CAOS-MIS group (0.3±0.5) was significantly lower (p&lt;0.01). Solid fusion was demonstrated in all the cases by dynamic radiographs. So it is feasible and safe for percutaneous minimally invasive C2-C3 pedicle screw fixation for Hangman's fracture using intra-operative three-dimensional fluoroscopy-based navigation, which can also decrease the incidence of post-operative neck pain

The evaluation of knee stability is fundamental for the clinical discrimination between healthy and pathological joints, for the design and evaluation of prostheses and for the definition of articular models. Knee stability can be quantified by measuring the relation between applied single-axis constant loads and corresponding tibio-femoral displacements (i.e., translations and rotations), namely the joint stiffness, at a given flexion angle. No many studies are available in the literature on this topic [1–3]. In particular, the translations/rotations along/about directions different from the loaded one were not deeply investigated. A fresh frozen lower-limb specimen (female, 63 years old, weight 68 Kg, height 158 cm) was considered. The forefoot and all soft tissues outside the knee were removed by a surgeon, keeping the knee joint capsule intact. A stereophotogrammetric system (Vicon Motion Systems Ltd.) was used to measure the femoro-tibial relative motion by two trackers fixed to the bones, thus introducing no soft-tissue artifact. The specimen was then mounted on a test rig capable to exert general loading conditions [4], and constant loads were applied to the tibia: ±100 N in antero-posterior (AP) and medio-lateral (ML) direction; ±10 Nm about abb-adduction (AA) and in-external (IE) rotations. Loads were applied approximately at the mid-point between the lateral and medial epicondyles, and were kept constant while the femur was flexed over a 135° range. Displacements were defined with respect to the joint natural motion (RTNM), also registered with the same rig. The relative motion of the bones was expressed by a standard joint coordinate system [5]. Considerable translations/rotations appeared also on different directions than the loaded one. At 90° of flexion, an anterior load of +100 N produced 5.5 mm of anterior translation, 10.9 mm of medial translation and 12° of external rotation of the tibia (RTNM). When not directly loaded in ML and IE directions, the tibia translated medially and rotated externally, independently from the sign of the applied load: at 90° of flexion, an AA torque of +10 Nm and −10 Nm produced respectively 5 mm and 8.9 mm of medial translation, and 5.5° and 7.5° of external rotation of the tibia (RTNM). The load/displacement relation was highly non linear also for the loading direction. At 90° of flexion, IE torques of +10 Nm and −10 Nm produced respectively 3.6° of internal and 14.2° of external rotation of the tibia (RTNM). The knee joint structures make the relation between applied loads and bone displacements highly non linear. As a result, a load acting on one direction produces a complex three-dimensional joint motion. Future work will extend the presented analysis on several specimens, also increasing the magnitude and the number of loading conditions

In cases of severe postdysplastic coxarthosis, it seems to be impossible to recognize acetabular geometry and the real femoral position on a plain X-ray because the real diameters and angles can be disfigured when projected. Computed tomography (CT) provides important information to the surgeon about the concavity, shape and stereotomy of the acetabulum. It is quite difficult to correctly evaluate severely changed hips. CT displays more precise acetabular diameters and angles than a plain radiograph. Because of the high density of bony tissue, the CT makes it easy to produce a three-dimensional display of the hip. From September 1995 to December 1998, 224 patients (148 female, 76 male) underwent arthroplasty using a non-cemented prosthesis according to Zweymüller. A total of 236 hip joints were operated and classified as Crowe Group I (76 hips), Crowe Group II (149 hips), and Crowe Group III (11 hips). There were no Crowe Group IV hips. A total of 96 patients were examined by 3D CT in preoperative planning. Based on CT results, four joints were not recommended for an operative solution. CT protocol: scanner Elscint TWIN II, slice 2.5 mm, 120 kV, 285 mAs, matrix 3402,. No. of slices: 40–50, incremental dual acquisition. Postprocessing: axial images, multiplanar reconstructions, 3D SSD. Acetabular stereometry: superoinferior diameter, anteroposterior diam., depth, bottom thickness, femoral neck anteversion angle and subtrochanteric marrow diameter. A three-dimensional CT of the hip is a very effective tool for preoperative assessment

The gelatin-based haemostyptic compound Spongostan was tested as a three-dimensional (3D) chondrocyte matrix in an in vitro model for autologous chondrocyte transplantation using cells harvested from bovine knees. In a control experiment of monolayer cultures, the proliferation or de-differentiation of bovine chondrocytes was either not or only marginally influenced by the presence of Spongostan (0.3 mg/ml). In monolayers and 3-D Minusheet culture chambers, the cartilage-specific differentiation markers aggrecan and type-II collagen were ubiquitously present in a cell-associated fashion and in the pericellular matrix. The Minusheet cultures usually showed a markedly higher mRNA expression than monolayer cultures irrespective of whether Spongostan had been present or not during culture. Although the de-differentiation marker type-I collagen was also present, the ratio of type-I to type-II collagen or aggrecan to type-I collagen remained higher in Minusheet 3-D cultures than in monolayer cultures irrespective of whether Spongostan had been included in or excluded from the monolayer cultures. The concentration of GAG in Minusheet cultures reached its maximum after 14 days with a mean of 0.83 ± 0.8 μg/10. 6. cells; mean ±, . sem. , but remained considerably lower than in monolayer cultures with/without Spongostan. Our results suggest that Spongostan is in principle suitable as a 3-D chondrocyte matrix, as demonstrated in Minusheet chambers, in particular for a culture period of 14 days. Clinically, differentiating effects on chondrocytes, simple handling and optimal formability may render Spongostan an attractive 3-D scaffold for autologous chondrocyte transplantation

Objectives. There remains a lack of data on the reliability of methods to estimate tibial coverage achieved during total knee replacement. In order to address this gap, the intra- and interobserver reliability of a three-dimensional (3D) digital templating method was assessed with one symmetric and one asymmetric prosthesis design. Methods. A total of 120 template procedures were performed according to specific rotational and over-hang criteria by three observers at time zero and again two weeks later. Total and sub-region coverage were calculated and the reliability of the templating and measurement method was evaluated. Results. Excellent intra- and interobserver reliability was observed for total coverage, when minimal component overhang (intraclass correlation coefficient (ICC) = 0.87) or no component overhang (ICC = 0.92) was permitted, regardless of rotational restrictions. Conclusions. Measurement of tibial coverage can be reliable using the templating method described even if the rotational axis selected still has a minor influence

Summary Statement. We successfully delineated the 3D micro morphology of chondrocytes in patella-patellar tendon using IL-XPCT for the first time. Compared with conventional histology, IL-XPCT can not only provide a higher resolution imgaing but also keep the 3D integrity of the specimen. Introduction. The morphology of the bone-tendon junction was complex and quite different from other organs, which result the injured bone-tendon junction repair process too slowly. To study the micro morphology of the bone-tendon junction in 3D may have a great significant value to revealing the repair mechanisms of this pathological process and accelerating injured bone-tendon junction repair. However, it was hindered by the convention methods such as histologic section. In our study, a novel imaging tool, synchrotron radiation based in-line x-ray phase contrast imaging (IL-XPCT) was used to research the 3D micro morphology of the bone-tendon junction. Methods. 1) Sample Preparation: 3 patella-patellar tendons was harvested from the knee joint of New Zealand adult rabbits and was immediately fixed, rinsed in water for 2 hours. Dehydration was done using a series of graded ethanol. The sample was cut out for the CCD pixel resolution in sagittal section. 2) Image Acquisition: The IL-XPCT was performed at the BL13W1 of the Shanghai Synchrotron Radiation Facility (SSRF) in China. The CCD pixel resolution was 0.74 μm. Image Acquisition include three steps, such as the the acquisition of tomo projections, CT slices and and 3D reconstruction of patella-patellar tendon on full scale by using VG Studio Max version 2.1. 3) Histological characterization observation: After scanning, the specimen was cut to histologic sectioning and used for morphology staining by safranin O staining and H&E staining. The histological morphology then compared with the IL-XPCT imaging dateset. Results. (1) The tissue gradations of patella-patellar tendon are clearly detected by IL-XPCT. (2) The 3D reconstruction image of patella-patellar tendon sample were largely match with the histological morphology stained by safranin O and H&E in sagittal view. (3) After the image segmentation, the 3D micro morphology of the bone-tendon junction could be vividly visualised in multi-angles. Through manipulate threshold of the 3D image, we can successfully obtained the 3D morphology of the chondrocyte, and the smallest diameter is approximately 5μm. Discussion & Conclusion. In the present study, we successfully delineated the 3D micro morphological features of chondrocytes in normal patella-patellar tendon using SR-based IL-XPCT for the first time. Compared with conventional histology, IL-XPCT can not only provide a higher resolution ratio without distortion but also keep the three-dimensional integrity of the specimen. Above all, IL-XPCT opens access to a new dimension in the morphological investigation of bone-tendon junction tissues, giving important complementary information to the conventional morphological analyses in view of the three-dimensional composition of bone-tendon junction tissues, On the other hand, it could be helpful to understanding the repair processes of bone-tendon junction injury and promoting the injured bone-tendon junction repair fast and high quality

A new method of recording the three-dimensional anatomy of the proximal femur from a single anteroposterior radiograph is described. This technique shows that in Perthes' disease the femoral head and neck are in significant anteversion and true varus. This anatomical configuration may be important in the pathogenesis and treatment of this disorder