Worldwide, most spine imaging is either “inappropriate” or “probably inappropriate”. The Choosing Wisely recommendation is “Do not perform imaging for lower back pain unless red flags are present.” There is currently no detailed breakdown of lower back pain diagnostic imaging performed in New Brunswick (NB) to inform future directions. A registry of spine imaging performed in NB from 2011-2019 inclusive (n=410,000) was transferred to the secure platform of the NB Institute for Data, Training and Research (NB-IRDT). The pseudonymized data included linkable institute identifiers derived from an obfuscated Medicare number, as well as information on type of imaging, location of imaging, and date of imaging. The transferred data did not include the radiology report or the test requisition. We included all lumbar, thoracic, and complete spine images. We excluded imaging related to the cervical spine, surgical or other procedures, out-of-province patients and imaging of patients under 19 years. We verified categories of X-ray, Computed Tomography (CT), and Magnetic Resonance Imaging (MRI). Red flags were identified by ICD-10 code-related criteria set out by the Canadian Institute for Health Information. We derived annual age- and sex-standardized rates of spine imaging per 100,000 population and examined regional variations in these rates in NB's two Regional Health Authorities (RHA-A and RHA-B). Age- and sex-standardized rates were derived for individuals with/without red flag conditions and by type of imaging. Healthcare utilization trends were reflected in hospital admissions and physician visits 2 years pre- and post-imaging. Rurality and socioeconomic status were derived using patients’ residences and income quintiles, respectively. Overall spine imaging rates in NB decreased between 2012 and 2019 by about 20% to 7,885 images per 100,000 people per year. This value may be higher than the Canadian average. Females had 23% higher average imaging rate than males. RHA-A had a 45% higher imaging rate than RHA-B. Imaging for red flag conditions accounted for about 20% of all imaging. X-rays imaging accounted for 67% and 75% of all imaging for RHA-A and RHA-B respectively. The proportions were 20% and 8% for CT and 13% and 17% for MRI. Two-year hospitalization rates and rates of physician visits were higher post-imaging. Females had higher age-standardized hospitalization and physician-visit rates, but the magnitude of increase was higher for males. Individuals with red flag conditions were associated with increased physician visits, regardless of the actual reason for the visit. Imaging rates were higher for rural than urban patients by about 26%. Individuals in the lowest income quintiles had higher imaging rates than those in the highest income quintiles. Physicians in RHA-A consistently ordered more images than their counterparts at RHA-B. We linked spine imaging data with population demographic data to look for variations in lumbar spine imaging patterns. In NB, as in other jurisdictions, imaging tests of the spine are occurring in large numbers. We determined that patterns of imaging far exceed the numbers expected for ‘red flag’ situations. Our findings will inform a focused approach in groups of interest. Implementing high value care recommendations pre-imaging ought to replace low-value routine imaging

Background. Routine imaging (radiography, CT, MRI) provides no health benefits for low back pain (LBP) patients and is not recommended in clinical practice guidelines. Whether imaging leads to increased costs, healthcare utilization or absence from work is unclear. Purpose. To systematically review if imaging in patients with LBP increases costs, leads to higher health care utilization or increases absence from work. METHODS. Randomized controlled trials (RCTs) and observational studies (OSs), comparing imaging versus no imaging on targeted outcomes were extracted from medical databases until October 2017. Data extraction and risk of bias assessment was performed independently by two reviewers. The quality of the body of evidence was determined using GRADE methodology. Results. Moderate quality evidence (1 RCT; n=421) supports that direct costs increase for patients undergoing radiography. Low quality evidence (3 OSs; n=9535) supports that early MRI leads to a large increase in costs. Moderate quality evidence (2 RCTs, 6 OSs; n=19392) supports that performing MRI, radiography or CT is associated with increased healthcare utilization. Two RCTs (n=667) showed no significant differences between radiography or MRI groups compared with no imaging groups on absence from work. However, the results of two observational studies (n=7765) did show significantly greater absence from work in the imaging groups compared to the no imaging-groups. Conclusions. Imaging in LBP is associated with higher medical costs and increased healthcare utilisation. There are indications that it also leads to higher absence from work. No conflicts of interest. No funding obtained

Pressure ulcers are a common occurrence in individuals with spinal cord injuries, and are attributed to prolonged sitting and limited mobility. This therefore creates the need to better understand soft tissue composition, in the attempt to prevent and treat pressure ulcers. In this study, novel approaches to imaging the soft tissue of the buttocks were investigated in the loaded and unloaded position using ultrasound (US) and magnetic resonance imaging (MRI). Twenty-six able-bodied participants (n=26, 13 males and 13 females) were recruited for this study and 1 male with a spinal cord injury. Two visits using US were required, as well as one MRI visit to evaluate soft tissue thickness and composition. US Imaging for the loaded conditions was performed using an innovative chair which allowed image acquisition in the seated upright position and MRI was done in the lateral decubitus position and loading was applied to the buttocks using a newly developed MRI compatible loader. The unloaded condition was a lateral decubitus position. Soft tissue was measured between the peak of the ischial tuberosity (IT) and the proximal femur and skin. Tissue thickness reliability for US was excellent, ICC=0.934–0.981 with no significant differences between the scan days. US and MRI measures of tissue thickness were significantly correlated (r=0.68–0.91). US underestimated unloaded tissue thicknesses with a mean bias of 0.39 – 0.56 for total tissue and muscle + tendon thickness. When the buttocks were loaded, total tissue thickness was reduced by 64.2±9.1%. US assessment of soft tissue thicknesses was reliable in both positions. The unloaded measurements using US were validated with MRI with acceptable limits of agreement, albeit tended to underestimate tissue thickness. Tissue thickness, but not fatty infiltration of muscle played a role in how the soft tissue of the buttocks responded to loading

Purpose: To review the MRI findings in transient osteoporosis of the hip (TOH) and to investigate the pattern of perfusion in dynamic studies. Material and Methods: Twenty-seven patients (29 hips), 23–66 years old, were referred for hip pain without history of trauma. In all patients the diagnosis of TOH based on x-rays (decrease bone density of the femoral head) and MRI (bone marrow edema-BME) was confirmed after complete resolution of symptoms and MRI findings after 6–18 months. MRI studies included T1-w SE, T2-w-SPIR-TSE and contrast enhanced T1-w TFE in dynamic mode and delayed SE. Imaging assessment included joint effusion, location and extent of BME (types A–D), sparing of the femoral head, subchondral linear lesions, and collapse. Results: Joint effusion was observed in 28 of 29 hips. The extent of BME in the femoral head was type A in 5/29 hips, B in 2/29, C in 16/29, D in 6/29. Associated BME of the acetabulum was depicted in 6/29 hips. In 12/29 hips the bone marrow edema was sparing the subchondral area. Subchondral line was only found in 2/29 hips. On dynamic T1–w images all hips presented with a delayed pattern of perfusion up to 40 sec. Conclusion: MRI findings are useful in diagnosing TOH and differentiating this entity from early AVN

The emergence of patient specific instrumentation has seen an expansion from simple radiographs to plan total knee arthroplasty (TKA) with modern systems using computed tomography (CT) or magnetic resonance imaging scans. Concerns have emerged regarding accuracy of these non-weight bearing modalities to assess true mechanical axis. The aim of our study was to compare coronal alignment on full length standing AP imaging generated by the EOS acquisition system with the CT coronal scout image. Eligible patients underwent unilateral or bilateral primary TKA for osteoarthritis under the care of investigating surgeon between 2017 and 2022, with both EOS X-Ray Imaging Acquisition System and CT scans performed preoperatively. Coronal mechanical alignment was measured on the supine coronal scout CT scan and the standing HKA EOS. Pre-operative lower limb coronal alignment was assessed on 96 knees prior to TKA on the supine coronal scout CT scan and the standing HKA EOS. There were 56 males (56%), and 44 right knees (44%). The mean age was 68 years (range 53-90). The mean coronal alignment was 4.7 degrees (SD 5.3) on CT scan and 4.6 degrees (SD 6.2) on EOS (p=0.70). There was a strong positive correlation of coronal alignment on CT scan and EOS (pearson. 0.927, p=0.001). The mean difference between EOS and CT scan was 0.9 degrees (SD 2.4). Less than 3 degrees variation between measures was observed in 87% of knees. On linear regression for every 1° varus increase in CT HKA alignment, the EOS HKA alignment increased by 0.93° in varus orientation. The model explained 86% of the variability. CT demonstrates excellent reliability for assessing coronal lower limb alignment compared to EOS in osteoarthritic knees. This supports the routine use of CT to plan TKA without further weight bearing imaging in routine cases

Abstract. Objectives. Investigate Magnetic Resonance Imaging (MRI) as an alternative to Computerised Tomography (CT) when calculating kinematics using Biplane Video X-ray (BVX) by quantifying the accuracy of a combined MRI-BVX methodology by comparing with results from a gold-standard bead-based method. Methods. Written informed consent was given by one participant who had four tantalum beads implanted into their distal femur and proximal tibia from a previous study. Three-dimensional (3D) models of the femur and tibia were segmented (Simpleware Scan IP, Synopsis) from an MRI scan (Magnetom 3T Prisma, Siemens). Anatomical Coordinate Systems (ACS) were applied to the bone models using automated algorithms. 1. The beads were segmented from a previous CT and co-registered with the MRI bone models to calculate their positions. BVX (60 FPS, 1.25 ms pulse width) was recorded whilst the participant performed a lunge. The beads were tracked, and the ACS position of the femur and tibia were calculated at each frame (DSX Suite, C-Motion Inc.). The beads were digitally removed from the X-rays (MATLAB, MathWorks) allowing for blinded image-registration of the MRI models to the radiographs. The mean difference and standard deviation (STD) between bead-generated and image-registered bone poses were calculated for all degrees of freedom (DOF) for both bones. Using the principles defined by Grood and Suntay. 2. , 6 DOF kinematics of the tibiofemoral joint were calculated (MATLAB, MathWorks). The mean difference and STD between these two sets of kinematics were calculated. Results. The absolute mean femur and tibia ACS position differences (Table 1) between the bead and image-registered poses were found to be within 0.75mm for XYZ, with all STD within ±0.5mm. Mean rotation differences for both bones were found to be within 0.2º for XYZ (Table 1). The absolute mean tibiofemoral joint translations (Table 1) were found to be within ±0.7mm for all DOF, with the smallest absolute mean in compression-distraction. The absolute mean tibiofemoral rotations were found to be within 0.25º for all DOF (Table 1), with the smallest mean was found in abduction-adduction. The largest mean and STD were found in internal-external rotation due to the angle of the X-rays relative to the joint movement, increasing the difficulty of manual image registration in that plane. Conclusion. The combined MRI-BVX method produced bone pose and tibiofemoral kinematics accuracy similar to previous CT results. 3. This allows for confidence in future results, especially in clinical applications where high accuracy is needed to understand the effects of disease and the efficacy of surgical interventions. Acknowledgements: This research was supported by the Engineering and Physical Sciences Research Council (EPSRC) doctoral training grant (EP/T517951/1). Declaration of Interest. (b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project

Title. Longitudinal Intravital Imaging to Quantify the “Race for the Surface” Between Host Immune Cell and Bacteria for Orthopaedic Implants with S. aureus Colonization in a Murine Model. Aim. To assess S. aureus vs. host cell colonization of contaminated implants vis intravital multiphoton laser scanning microscopy (IV-MLSM) in a murine model. Method. All animal experiments were approved by IACUC. A flat stainless steel or titanium L-shaped pin was contaminated with 10. 5. CFU of a red fluorescent protein (RFP) expressing strain of USA300LAC, and surgically implanted through the femur of global GFP-transgenic mice. IV-MLSM was performed at 2, 4, and 6 hours post-op. Parallel cross-sectional CFU studies were performed to quantify the bacteria load on the implant at 2,4,6,12,18 and 24 hours. Results. 1) We developed a high-fidelity reproducible IV-MLSM system to quantify S. aureus and host cell colonization of a bone implant in the mouse femur. Proper placement of all implants were confirmed with in vivo X-rays, and ex vivo photos. We empirically derive the ROI during each imaging session by aggregating the imaged volume which ranges from (636.4um × 636.4um × 151um) = 0.625 +/- 0.014 mm. 3. of bone marrow in a global GFP-transgenic mouse. 2) IV-MLSM imaging acquisition of the “race for the surface”.In vitro MPLSM images of implants partially coated with USA300LAC (RFP-MRSA) were verified by SEM image. Results from IV-MLSM of RFP-MRSA and GFP. +. host cell colonization of the contaminated implants illustrated the mutually exclusive surface coating at 3hrs, which to our knowledge is the first demonstration of “the race for the surface” between bacteria and host cells via intravital microscopy. 3) Quantifying the “race for the surface” with CFU verification of S. aureus on the implant. 3D volumetric rendering of the GFP. +. voxels and RFP+ voxels within the ROI were generated in Imaris. The voxel numbers suggeste that the fight for the surface concludes ∼3hrs post-infection, and then transitions to an aggressive MRSA proliferation phase. The results of WT control demonstrate a significant increase in CFU by 12hrs post-op for both stainless steel (P<0.01) and titanium (P<0.01). Conclusions. We developed IV-MLSM to quantify the “Race for the Surface” between host cells and contaminating S. aureus in a murine femur implant model. This race is remarkably fast, as the implant surface is completely covered with 3hrs, peak bacterial growth on the implant occurs between 2 and 12 hours and is complete by 12hrs

In foot and ankle surgery incorrect placement of implants, or inaccuracy in fracture reduction may remain undiscovered with the use of conventional C-arm fluoroscopy. These imperfections are often only recognized on postoperative computer tomography scans. The apparition of three dimensional (3D) mobile Imaging system has allowed to provide an intraoperative control of fracture reduction and implant placement. Three dimensional computer assisted surgery (CAS) has proven to improve accuracy in spine and pelvic surgery. We hypothesized that 3D-based CAS could improve accuracy in foot and ankle surgery. The purpose of our study was to evaluate the feasibility and utility of a multi-dimensional surgical imaging platform with intra-operative three dimensional imaging and/or CAS in a broad array of foot and ankle traumatic and orthopaedic surgery. Cohort study of patients where the 3D mobile imaging system was used for intraoperative 3D imaging or 3D-based CAS in foot and ankle surgery. The imaging system used was the O-arm Surgical Imaging System and the navigation system was the Medtronic's StealthStation. Surgical procedures were performed according to standard protocols. In case of fractures, image acquisition was performed after reduction of the fracture. In cases of 3D-based CAS, image acquisition was performed at the surgical step before implants placement. At the end of the operations, an intraoperative 3D scan was made. We used the O-arm Surgical Imaging system in 11 patients: intraoperative 3D scans were performed in 3 cases of percutaneus fixation of distal tibio-fibular syndesmotic disruptions; in 2 of the cases, revision of reduction and/or implant placement were needed after the intraoperative 3D scan. Three dimensional CAS was used in 10 cases: 2 open reduction and internal fixation (ORIF) of the calcaneum, 1 subtalar fusion, 2 ankle arthrodesis, 1 retrograde drilling of an osteochondral lesion of the talus, 1 Charcot diabetic reconstruction foot and 1 intramedullary screw fixation of a fifth metatarsal fracture. The guidance was used essentially for screw placement, except in the retrograde drilling of an osteochondral lesion where the guidance was used to navigate the drill tool. Intraoperative 3D imaging showed a good accuracy in implant placement with no need to revision of implants. We report a preliminary case series with use of the O-arm Surgical Imaging System in the field of foot and ankle surgery. This system has been used either as intraoperative 3D imaging control or for 3D-based CAS. In our series, the 3D computer assisted navigation has been very useful in the placement of implants and has shown that guidance of implants is feasible in foot and ankle surgery. Intraoperative 3D imaging could confirm the accuracy of the system as no revisions were needed. Using the O-arm as intraoperative 3D imaging was also beneficial because it allowed todemonstrate intraoperative malreduction or malposition of implants (which were repositioned immediately). Intraoperative 3D imaging system showed very promising preliminary results in foot and ankle surgery. There is no doubt that intraoperative use of 3D imaging will become a standard of care. The exact indications need however to be defined with further studies

Introduction. Cruciate retaining knee replacements are only implanted into patients with “healthy” ligaments. However, partial anterior cruciate ligament (ACL) tears are difficult to diagnose with conventional MRI. Variations of signal intensity within the ligament are suggestive of injury but it is not possible to confirm damage or assess the collagen alignment within the ligaments. The potential use of Magic Angle Directional Imaging (MADI) as a collagen contrast mechanism is not new, but has remained a challenge. In theory, ligament tearing or joint degeneration would decrease tissue anisotropy and reduce the magic angle effect. Spontaneous cruciate ligament rupture is relatively common in dogs. This study presents results from ten canine knees. Methods. Ethical approval was obtained to collect knees from euthanized dogs requiring a postmortem (PM). A Siemens Verio 3T MRI scanner was used to scan a sphere containing the canine knees in 9 directions to the main magnetic field (B. 0. ) with an isotropic 3D-T1-FLASH sequence. After imaging, the knees were dissected and photographed. The images were registered and aligned to compare signal intensity variations. Segmentation using a thresholding technique identified voxels containing collagen. For each collagen-rich voxel the orientation vector was computed using Szeverenyi and Bydder's method. Each orientation vector reflects the net effect of all fibers comprised within a voxel. The assembly of all unit vectors represents the fiber orientation map and was visualised in ParaView using streamlines. The Alignment Index (AI) is defined as a ratio of the fraction of orientations within 20° (solid angle) centred in that direction to the same fraction in a random (flat) case. By computing AI for a regular gridded orientation space we can visualise differences in AI on a hemisphere. AI was normalised so that AI=0 indicates isotropic collagen alignment. Increasing AI values indicate increasingly aligned structures: AI=1 indicates that all collagen fibers are orientated within the cone of 20° centred at the selected direction. Results. Dogs cranial cruciate ligament (CCL) is similar to human ACL. It's composed of an anteromedial (AM) bundle and a posterolateral (PL) bundle. Two knees were damaged with partial CCL tears, the PL bundle was intact but the AM bundle was torn. Paraview streamlines of the CCL for healthy and damaged knees differ. The healthy knee has continuous fiber tracts with no ligament disruption. In the AM bundle fibers are discontinuous and the PL bundle fibers are continuous as expected in a partially torn CCL. The AI for healthy (mean AI=0.25) and damaged CCL (mean AI= 0.075) is significantly different (p<0.01). The damaged AM bundle has a more diffuse spread of less aligned fibers compared to the more concentrated and aligned PL fiber bundle. Conclusion. This study demonstrates the first visualisation of a CCL partial tear using MADI. Combined with AI, our scanning technique offers a tool to visualise and quantify changes in collagen fiber orientation. Thus, MRI can be used to improve the diagnosis and quantification of partial ligament tears in the knee

Background

An improved understanding of intervertebral disc (IVD) structure and function is required for treatment development. Loading induces micro-fractures at the interface between the nucleus pulposus (NP) and the annulus fibrosus (AF), which is hypothesized to induce a cascade of cellular changes leading to degeneration. However, there is limited understanding of the structural relationship between the NP and AF at this interface and particularly response to load. Here, X-ray scattering is utilised to provide hierarchical morphometric information of collagen structure across the IVD, especially the interface region under load.

Abstract

Background. Digital templating is a critical part of preoperative planning for total hip arthroplasty (THA) that is increasingly used by orthopaedic surgeons as part of their preoperative planning process. Digital templating has been used as a method of reducing hospital costs by eliminating the need for acetate films and providing an accurate method of preoperative planning. Pre-operative templating can help anticipate and predict appropriate component sizes to help avoid postoperative leg length discrepancy, failure to restore offset, femoral fracture, and instability. A preoperative plan using digital radiographs for surgical templating for component size can improve intraoperative accuracy and precision. While templating on conventional and digital radiographs is reliable and accurate, the accuracy of templating on digital images acquired with a novel biplanar imaging system (EOS Imaging Inc, Cambridge, MA, USA) remains unknown. EOS imaging captures whole body images of a standing patient without stitching or vertical distortion, less magnification error and exposes patients to less radiation than a pelvis AP radiograph. Therefore, the purpose of this study was to compare EOS imaging and conventional anteroposterior (AP) xrays for preoperative digital templating for THA, and compare the results to the implant sizes used intraoperatively. Methods. Forty primary unilateral THA patients had preoperative supine AP xrays and standing EOS imaging. The mean age for patients was 61 ± 8 years, the mean body mass index 29 ± 6 kg/m. 2. and 21 patients were female. All patients underwent a THA with the same THA system (R3 Acetabular System and Synergy Cementless Stem, Smith & Nephew, TN, USA) by a single surgeon. Two blinded observers preoperatively templated using both AP xray and EOS imaging for each patient to predict acetabular size, femoral component size, and stem offset. All templating was performed by two observers with standard software (Ortho Toolbox, Sectra AB, Linköping, Sweden) [Figure 1] one week prior to surgery, and were compared using the Cronbach's alpha (∝) coefficient of reliability. The accuracy of templating was reported as the average percent agreement between the implanted size and the templated size for each component. Results. For templating acetabular component size, the exact size was predicted for 48% using AP xrays and 70% using EOS imaging, and within 1 size for 88% using xrays and 98% using EOS imaging. For templating femoral component size, the exact size was predicted exactly for 33% using AP xrays and 60% using EOS imaging, and within 1 size for 85% using xrays and 98% using EOS imaging (Figure 2). Interobserver agreement was excellent for acetabular components (Cronbach's α = 0.94) and femoral components (Cronbach's α = 0.96) using EOS imaging. Conclusions. This study demonstrates that preoperative digital templating for THA using EOS imaging is accurate, with excellent interobserver agreement. EOS imaging has less magnification error, which may partially explain the accuracy of our templating method

Introduction. Implant position plays a major role in the mechanical stability of a total hip replacement. The standard modality for assessing hip component position postoperatively is a 2D anteroposterior radiograph, due to low radiation dose and low cost. Recently, the EOS® X-Ray Imaging Acquisition System has been developed as a new low-dose radiation system for measuring hip component position. EOS imaging can calculate 3D patient information from simultaneous frontal and lateral 2D radiographs of a standing patient without stitching or vertical distortion, and has been shown to be more reliable than conventional radiographs for measuring hip angles[1]. The purpose of this prospective study was to compare EOS imaging to computer tomography (CT) scans, which are the gold standard, to assess the reproducibility of hip angles. Materials and Methods. Twenty patients undergoing unilateral THA consented to this IRB-approved analysis of post-operative THA cup alignment. Standing EOS imaging and supine CT scans were taken of the same patients 6 weeks post-operatively. Postoperative cup alignment and femoral anteversion were measured from EOS radiographs using sterEOS® software. CT images of the pelvis and femur were segmented using MIMICS software (Materialise, Leuven, Belgium), and component position was measured using Geomagic Studio (Morrisville, NC, USA) and PTC Creo Parametric (Needham, MA). The Anterior Pelvic Plane (APP), which is defined by the two anterior superior iliac spines and the pubic symphysis, was used as an anatomic reference for acetabular inclination and anteversion. The most posterior part of the femoral condyles was used as an anatomic reference for femoral anteversion. Two blinded observers measured hip angles using sterEOS® software. Reproducibility was analysed by the Bland-Altman method, and interobserver reliability was calculated using the Cronbach's alpha (∝) coefficient of reliability. Results. The Bland-Altman analysis of test-retest reliability indicated that the 95% limits of agreement between the EOS and CT measurements ranged from −3° to 4° for acetabular inclination, from −5° to 5° for acetabular anteversion, and from −7° to 2° for femoral anteversion. The average difference between EOS measurements and CT measurements was 2° ± 2° for acetabular inclination, 3°± 2° degrees for acetabular anteversion and 4° ± 4° femoral anteversion. Interobserver agreement was good for acetabular inclination (Cronbach's α = 0.55), acetabular anteversion (Cronbach's α = 0.76) and femoral components (Cronbach's α = 0.98) using EOS imaging. Conclusions. EOS imaging can accurately and reliably measure hip component position, while exposing patients to a much lower dose of radiation than a CT scan

Introduction

The various problems that are managed with circular external fixation (e.g. deformity, complex fractures) also typically require serial plain x-ray imaging.

One of the challenges here is that the relatively radio-opaque components of the circular external fixator (e.g. the rings) can obscure the view of the area of interest (e.g. osteotomy site, fracture site).

In this presentation we describe how the geometry of the x-ray beam affects the produced image and how we can use knowledge of this to our advantage.

Whilst this can be applied to any long bone, we have focused on the tibia, given that it's the most common long bone that is treated by circular external fixation.

Aims

There is no consensus regarding optimum timing and frequency of ultrasound (US) for monitoring response to Pavlik harness (PH) treatment in developmental dysplasia of the hip (DDH). The purpose of our study was to determine if a limited-frequency hip US assessment had an adverse effect on treatment outcomes compared to traditional comprehensive US monitoring.

Background. Total knee arthroplasty (TKA) is a cost-effective surgical procedure for degenerative knee disease and has good long-term results. However, these results are not always related to patient satisfaction and functional outcome. With an increasing demand of surgeons and patients on functioning of total knee implants, the need for adequate objective outcome measures is high. Imaging of the knee is commonly used in clinical practice and research to objectively measure many different outcome parameters concerning the implant, such as alignment and complications.1 However, techniques on comparison of the sagittal contour of the knee before and after implant placement are scarce. Goal. To develop and describe a standardized method for measuring the sagittal contour of the implant in a 3D model of the knee before and after implant placement. Methods. Images of the static knee of a subject are obtained in-vivo using fluoroscopy over a 180° sweep at 15 frames per second (MultiDiagnost Eleva, Philips, The Netherlands). A 3D model of the knee is constructed in accompanying software (3D-RX, Philips, The Netherlands) and is subsequently imported in OsiriX imaging software (Pixmeo, Switzerland). In Osirix, a reproducible coordinate system is obtained using the bone stub axis and the anatomical epicondylar axis as references [Fig. 1]. We quantified the sagittal contour of the distal femur in two parameters: the flexion angle of femoral component and the sagittal profile of the implant. To measure the flexion angle, the image is located in the midtrochlear plane. The angle is measured between the bone stub axis and the neutral line of the femoral component [Fig. 2]. To measure the sagittal profile of the distal femur, the lengths of three lines connecting the anatomical epicondylar axis of the distal femur and the outer border of the femur/prosthesis are summed. This is done both anterior and posterior [Fig. 3]. These profiles are measured in planes of the lateral and medial condyle and of the midtrochlear plane. Due to the reproducible coordinate system, the profiles can be compared for the knee before and after implant placement. Conclusion. Using fluoroscopy and readily available 3D imaging software we have developed a technique for measuring valuable parameters concerning implant placement in TKA. This technique can be used for scientific purposes concerning comparison of the knee before and after implant placement and to study its effect on functional and biomechanical outcome after TKA

Imaging techniques including MR scanning and ultrasound were discussed. However it was noted that for standard orthopaedic practice in the UK plain films were the initial imaging available. The importance of taking a skyline view was stressed. The Dejour protocol was then outlined where the lateral Xray of the knee assesses the patellar height, the presence of dysplasia of the trochlear groove, and, if present, its boss height. A CT scan defines the patellar tilt angle, and with cuts through the tibial tubercle, the offset of the tibial tubercle from the trochlear groove (TTTG). The four abnormalities that can be defined are then corrected at operation. All patients undergo a lateral release. If the patellar tilt angle is greater than 20° then a medial reefing is performed. If the patellar height is greater than 1.2, a distalisation of the tibial tubercle to correct this to 1.0 is done. A MG of greater than 2Omm leads to a correction by an Elmsie medial tubercle transfer. A boss height of greater than 6mm suggests a trochleoplasty should be performed. However the Dejour protocol is yet to be validated. It was concluded that imaging is essential for analysing patellofemoral instability. Plain films alone do not give enough information. Patterns of patellofemoral instability as assessed by CT scan (and MRI scan) are yet to be established. Postoperative imaging to confirm correction of abnormalities should be done. The measurements are worthwhile but their validation is awaited

Literature surrounding artificial intelligence (AI)-related applications for hip and knee arthroplasty has proliferated. However, meaningful advances that fundamentally transform the practice and delivery of joint arthroplasty are yet to be realized, despite the broad range of applications as we continue to search for meaningful and appropriate use of AI. AI literature in hip and knee arthroplasty between 2018 and 2021 regarding image-based analyses, value-based care, remote patient monitoring, and augmented reality was reviewed. Concerns surrounding meaningful use and appropriate methodological approaches of AI in joint arthroplasty research are summarized. Of the 233 AI-related orthopaedics articles published, 178 (76%) constituted original research, while the rest consisted of editorials or reviews. A total of 52% of original AI-related research concerns hip and knee arthroplasty (n = 92), and a narrative review is described. Three studies were externally validated. Pitfalls surrounding present-day research include conflating vernacular (“AI/machine learning”), repackaging limited registry data, prematurely releasing internally validated prediction models, appraising model architecture instead of inputted data, withholding code, and evaluating studies using antiquated regression-based guidelines. While AI has been applied to a variety of hip and knee arthroplasty applications with limited clinical impact, the future remains promising if the question is meaningful, the methodology is rigorous and transparent, the data are rich, and the model is externally validated. Simple checkpoints for meaningful AI adoption include ensuring applications focus on: administrative support over clinical evaluation and management; necessity of the advanced model; and the novelty of the question being answered.

Cite this article: Bone Joint J 2022;104-B(12):1292–1303.

Shoulder Instability impacts on the ability of military personnel to fulfil their operational role and maintain sporting competence. Magnetic Resonance Imaging (MRI) and Arthrogram (MRA) are increasingly available as diagnostic adjuncts. We analysed MR reports from personnel undergoing stabilisation, correlating clinical diagnosis with operative findings and reviewed the literature in order to recommend improvements. We report a retrospective, consecutive case note analysis of 106 personnel undergoing open anterior capsulolabral reconstruction (ACLR) by a single surgeon. Seventy patients had MR (48 MRA, 22 MRI). Commonly reported pathology included Hill Sachs Lesions (41%), Soft tissue (59%) and bony Bankart lesions (16%), capsular laxity (20 %), supraspinatus lesion (20%), ACJ disease (13%) and SLAP tear (12%). The sensitivity of MR for identification of labral lesions was 82% with a specificity of 86%. For bony glenoid lesions, sensitivity was 63% and specificity 94%. Disparity between report and operative findings occurred mainly in standard MRI. Patients with lesions unrelated to instability achieved a pain free functioning shoulder following stabilisation. 100% of patients referred for ACLR with clinical evidence of instability without MR had positive pre-operative and operative correlation with instability. In 5 cases, the original equivocal MRI was repeated by MRA due to clinical suspicion of instability. In all cases the repeat MRA correlated with pre-operative and operative findings of instability. In 5 cases with equivocal clinical findings, MRA provided confirmation of instability. Delay in referral due to scanning and follow up ranged from 0 – 15 weeks. The diagnosis of those instigating referral is accurate. Reporting of MR is open to variation and has cost implications. MRA performed by a radiologist with a musculoskeletal specialist interest is recommended on an individual basis only and routine use of non-arthrographic studies should be discontinued. This will improve the efficiency of the fast track pathway

Primary care physicians rely on radiology reports to confirm a scoliosis diagnosis and inform the need for spine specialist referral. In turn, spine specialists use these reports for triage decisions and planning of care. To be a valid predictor of disease and management, radiographic evaluation should include frontal and lateral views of the spine and a complete view of the pelvis, leading to accurate Cobb angle measurements and Risser staging. The study objectives were to determine 1) the adequacy of index images to inform treatment decisions at initial consultation by generating a score and 2) the utility of index radiology reports for appropriate triage decisions, by comparing reports to corresponding images.

We conducted a retrospective chart and radiographic review including all idiopathic scoliosis patients seen for initial consultation, aged three to 18 years, between January 1-April 30, 2021. A score was generated based on the adequacy of index images to provide accurate Cobb angle measurements and determine skeletal maturity (view of full spine, coronal=two, lateral=one, pelvis=one, ribcage=one). Index images were considered inadequate if repeat imaging was necessary. Comparisons were made between index radiology report, associated imaging, and new imaging if obtained at initial consultation. Major discrepancies were defined by inter-reader difference >15°, discordant Risser staging, or inaccuracies that led to inappropriate triage decisions. Location of index imaging, hospital versus community-based private clinic, was evaluated as a risk factor for inadequate or discrepant imaging.

There were 94 patients reviewed with 79% (n=74) requiring repeat imaging at initial consultation, of which 74% (n=55) were due to insufficient quality and/or visualization of the sagittal profile, pelvis or ribcage. Of index images available for review at initial consult (n=80), 41.2% scored five out of five and 32.5% scored two or below. New imaging showed that 50.0% of those patients had not been triaged appropriately, compared to 18.2% of patients with a full score. Comparing index radiology reports to initial visit evaluation with <60 days between imaging (n=49), discrepancies in Cobb angle were found in 24.5% (95% CI 14.6, 38.1) of patients, with 18.4% (95% CI 10.0, 31.4) categorized as major discrepancies. Risser stage was reported in only 14% of index radiology reports. In 13.8% (n=13) of the total cohort, surgical or brace treatment was recommended when not predicted based on index radiology report. Repeat radiograph (p=0.001, OR=8.38) and discrepancies (p=0.02, OR=7.96) were increased when index imaging was obtained at community-based private clinic compared to at a hospital. Re-evaluation of available index imaging demonstrated that 24.6% (95% CI 15.2, 37.1) of Cobb angles were mis-reported by six to 21 degrees.

Most pre-referral paediatric spine radiographs are inadequate for idiopathic scoliosis evaluation. Standardization of spine imaging and reporting should improve measurement accuracy, facilitate triage and decrease unnecessary radiation exposure.

Dupuytrens disease is a fibrosing condition of the palmar aponeurosis and its extensions within the digits. Normal fascial fibres running longitudinally in the subcutaneous tissues of the palm become thickened and form the characteristic nodules and cords pathognomonic of Dupuytrens disease. A wide variety of surgical interventions exist, of these the partial fasciectomy remains the most conventional and widely used technique. Minimally invasive surgical treatments such as needle fasciotomy are, however, becoming increasingly popular. Dupuytrens disease remains a challenging condition to treat as recurrence is universally found with all surgical interventions. Although recurrence may be related to the severity of the disease, there are currently no research tools other than clinical examination to examine changes in the diseased tissue postoperatively and predict likelihood of long-term success. Magnetic Resonance Imaging (MRI) may be of value for the study of Dupuytren disease, at present its use has been greatly underexplored. We wished to carry out a pilot study in order to examine the possibility of using 3.0 Tesla MRI to study Dupuytren tissue and then furthermore to examine the potential changes post-operatively following percutaneous fasciotomy. Five patients set to undergo percutaneous needle fasciotomy were recruited and consented for the study. All patients underwent MRI scanning of the affected hand pre-operatively and at two weeks post-operatively. Scanning was carried out in the 3.0 Tesla research MRI scanner at Aberdeen Royal Infirmary. Patients were placed prone in the MRI scanner with the hand outstretched above the head in the so-called “Superman” position. A specially designed wrist and hand coil was used. Under the expertise of radiographers and physicists, image capture encompassed four novel scanning sequences in order to make a volumetric three-dimensional image sample of the affected hand. MIPAV software (Bethesda, Maryland) was used for image analysis. Scanning revealed well defined anatomy. The Dupuytren cord arose from the palmar aponeurosis tissue which is deep to the palmar skin and subcutaneous tissue. It was distinctly different to deep structures such as the flexor tendons and intrinsic hand muscles which appeared with a uniform low and high signal respectively. The Dupuytren tissue had a heterogeneous signal on both T1 and T2 images. On T1 the tissue signal appeared high to intermediate, similar to that of bone and muscle, but low areas of signal were observed diffusely in an irregular fashion throughout. On T2 the tissue had a low signal throughout with some focal areas of high signal. Dupuytren tissue was mapped using MIPAV software for pre- and post-operative comparisons. Signal intensity, surface area and volume of the cords and fasciotomy sites were explored. Our initial results suggest MRI can be used to study Dupuytren tissue. Such a research tool may be of use to study the natural history of Dupuytren disease and furthermore, the response to medical and surgical interventions

X-ray is the standard method for monitoring fracture healing however it is not ideal; signs of healing are not normally visible on X-ray until around 6–8 weeks post fracture. Ultrasonography allows the detection of both the initial haematoma, usually formed immediately after fracture, and the small calcium deposits laid down between broken bone ends in the first stages of fracture healing. It has been reported that these early indicators of the healing process are visible as early as 1–2 weeks after fracture. We use Freehand 3D Ultrasound to monitor the early stages of fracture healing as both the bone surface and surrounding soft tissues can be imaged simultaneously. The Freehand 3D Ultrasound system consists of a standard Ultrasound machine, a PC running STRAD-WIN (Medical Imaging Group, Cambridge University) 3D software, and an optical tracking devise (NDI Polaris) to record the position and orientation of the Ultrasound probe during scanning. Images are transferred from the Ultrasound machine to the PC using RF capture through out a scan. Calibrating the system matches up the correct image with the correct probe position to produce a 3D dataset. We segment features of interest on the sequence of 2D images to construct a 3D model. These models are rotatable and provide views of the scanned anatomy that are not otherwise achievable using conventional Ultrasound or X-ray. The 3D data set can also be resliced through any plane to provide further views. To conduct a 3D Ultrasound scan takes the same amount of time as a conventional 2D scan. The production of the 3D model takes between 15–60 minutes depending on the level of detail required. Distances are measurable to within ±0.4mm meaning fracture gaps of sub-millimeter width can be resolved. The system has already been evaluated on healthy volunteers and a clinical study currently underway

Shoulder Instability impacts on the ability of military personnel to fulfil their operational role and maintain sporting competence. Magnetic Resonance Imaging (MRI) and Arthrogram (MRA) are increasingly available as diagnostic adjuncts. We analysed MR reports from personnel undergoing stabilisation, correlating clinical diagnosis with operative findings and reviewed the literature in order to recommend improvements. This was a retrospective, consecutive case note analysis of 106 personnel undergoing open anterior capsulolabral reconstruction (ACLR) by a single surgeon. 70 patients had MR (48 MRA, 22 MRI). Commonly reported pathology included Hill Sachs Lesions (41%), Soft tissue (59%) and bony Bankart lesions (16%), capsular laxity (20 %), supraspinatus lesion (20%), ACJ disease (13%) and SLAP tear (12%). The sensitivity of MR for identification of labral lesions was 82% with a specificity of 86%. For bony glenoid lesions, sensitivity was 63% and specificity 94%. Disparity between report and operative findings occurred mainly in standard MRI. Patients with lesions unrelated to instability achieved a pain free functioning shoulder following stabilisation. 100% of patients referred for ACLR with clinical evidence of instability without MR had positive pre-operative and operative correlation with instability. In 5 cases, the original equivocal MRI was repeated by MRA due to clinical suspicion of instability. In all cases the repeat MRA correlated with pre-operative and operative findings of instability. In 5 cases with equivocal clinical findings, MRA provided confirmation of instability. Delay in referral due to scanning and follow up ranged from 0–15 weeks. The diagnosis of those instigating referral is accurate. Reporting of MR is open to variation and has cost implications. MRA performed by a radiologist with a musculoskeletal specialist interest is recommended on an individual basis only and routine use of non-arthrographic studies should be discontinued. This will improve the efficiency of the fast track pathway

Aim. The aim of this study was to compare the diagnostic accuracy of the Magnetic Resonance Imaging with that of Stress views of the ankle in testing the integrity of the lateral ankle ligaments. Arthroscopic diagnosis was used as the gold standard. Methods. This was a prospective study involving 45 patients who had previous trauma to the ankle and reported symptoms of ankle instability. Our patients were recreational athletes or military patients. These patients had MRI evaluation prior to arthroscopic evaluation and treatment of the ankle. The diagnosis regarding the integrity of the Calcaneofibular ligament (CFL) and the Anterior Talo-fibular ligament (ATFL), as obtained from the MRI was compared against the assessment of integrity from the stress views. These were compared against the assessment made by direct visualisation of the ligaments during arthroscopy. The sensitivity, specificity, negative (NPV) and positive predictive values (PPV) and accuracy were then calculated. Results. The sensitivity and specificity of the MRI and the stress views were poor for diagnosis of ATFL tears. However, the stress views had better sensitivity (93.7%) and specificity (96.5%), for the CFL, as compared with those of the Magnetic Resonance scans (sensitivity 50% and specificity of 86.2%). There was a difference between the diagnostic accuracy of the two methods of investigation with respect to integrity of the CFL but not of that of the ATFL. The PPV and the NPV for the ATFL was comparable using the MRI and the stress radiographs, the stress radiographs had a better predictive values for the calcaneo-fibular ligament, PPV of 93.7% and NPV of 96.5%. Conclusion. The results of this study suggest that routine pre-operative Magnetic Resonance Imaging is not beneficial or cost effective in diagnosing lateral ligament

Magnetic Resonance Imaging is increasingly utilised for the assessment of knee pathology. The aim of this study was to review our entire knee MRI scans and to assess the accuracy of diagnosis when compared with operative diagnosis. Using data from the radiology department and medical records (public and private) all patients having knee MRI scans in a 6-year period were identified. There were 956 scans performed on 930 patients. Scan diagnosis, operative diagnosis and diagnostic accuracy were assessed. Of the scanned patients 181 (19.5%) had normal scans and of these 168 (92.8%) were accurately diagnosed as normal. The remaining 749 (80.5%) had an abnormality noted on scan and of these 298 (39.8%) proceeded on to surgery. Of those patients having surgery, diagnosis at surgery was found to exactly match the results of the scan in 163 (57.0%) patients. Furthermore 51.5% of patients with a diagnosis of meniscal degeneration by scan actually had a meniscal tear at operation. However the sensitivity for diagnosis of ACL tears was 89.0% and that of medial meniscal tears was 90.6%. MRI diagnosis is far from infallible and clinicians should be conscious of its limitations. However it is particularly reliable in confirming the lack of pathology within a knee with an accuracy of 93%. It also has high sensitivity for diagnosis of ACL and meniscal tears

Introduction:. Unicompartmental knee arthroplasty (UKA) is a well established method for treatment of single compartment arthritis. However, a subset of patients still present with continued pain after their procedure in the setting of a normal radiographic examination. We propose the use of magnetic resonance imaging (MRI) as a useful modality in determining the etiology of symptoms in symptomatic unicompartmental knee arthroplasties. Materials & Methods:. An IRB-approved retrospective analysis of 300 consecutive unicompartmental knee arthroplasties between 2008–2010 found 28 cases symptomatic for continued pain. Magnetic resonance imaging was performed with a 1.5 T Surface Coil unit after clinical and radiographic assessment. MRI evaluation included assessment for osteoarthritis, synovitis, osteolysis, and loosening. Validated questionnaires including PAQ, WOMAC and UCLA Activity Score were used for clinical assessment. Results:. The average age at surgery was 56.1 ± 10.9 years (34–79). Imaging results indicated progressive arthritis in 28 patients (100%), synovitis in 17 patients (61%), osteolysis in 9 patients (32.0%), and loosening in 3 patients (11%). Based on these results and other clinical findings, a revision or conversion to a TKR was advised for 10 patients and 18 were recommended for nonoperative therapies. One patient received treatment at a separate hospital, and another was lost to follow-up. At post-operative follow-up of 1.4 ± 0.9 years, 7 of the 10 patients (70%) in the operative group experienced improvement in pain and function. The mean PAQ, UCLA and WOMAC index scores for these patients were 8.0 ± 1.4 (7–9), 5.5 ± 6.4 (1–10), and 2.0 ± 2.8 (0–4), respectively. In the nonoperative group, 11 of the 18 patients (61%) experienced improvement in pain and function. Among these patients, the mean PAQ, UCLA and WOMAC index scores were 7.6 ± 3.7 (0–10), 5.9 ± 3.2 (1–10) and 8.9 ± 12.0 (0–28), respectively. Conclusion:. The use of MRI as an imaging modality for symptomatic arthroplasty patients is becoming more commonly used. This study shows how MRI with sound clinical judgment can influence treatment decisions and supports the use of high quality MRI as a diagnostic tool for the symptomatic unicompartmental knee arthroplasty

Cartilage diseases have a significant impact on the patient's quality of life and are a heavy burden for the healthcare system. Better understanding, early detection and proper follow-up could improve quality of life and reduce healthcare related costs. Therefore, the aim of this study was to evaluate if difference between osteoarthritic (OA) and non-osteoarthritic (non-OA) knees can be detected quantitatively on cartilage and subchondral bone levels with advanced but clinical available imaging techniques.

Two OA (mean age = 88.3 years) and three non-OA (mean age = 51.0 years) human cadaveric knees were scanned two times. A high-resolution peripheral quantitative computed tomography (HR-pQCT) scan (XtremeCT, Scanco Medical AG, Switzerland) was performed to quantify the bone microstructure. A contrast-enhanced clinical CT scan (GE Revolution Evo, GE Medical Systems AG, Switzerland) was acquired with the contrast agent Visipaque 320 (60 ml) to measure cartilage. Subregions dividing the condyle in four parts were identified semi-automatically and the images were segmented using adaptive thresholding. Microstructural parameters of subchondral bone and cartilage thickness were quantified.

The overall cartilage thickness was reduced by 0.27 mm between the OA and non-OA knees and the subchondral bone quality decreased accordingly (reduction of 33.52 % in BV/TV in the layer from 3 to 8 mm below the cartilage) for the femoral medial condyle. The largest differences were observed at the medial part of the femoral medial condyle both for cartilage and for bone parameters, corresponding to clinical observations.

Subchondral bone microstructural parameters and cartilage thickness were quantified using in vivo available imaging and apparent differences between the OA and non-OA knees were detected. Those results may improve OA follow-up and diagnosis and could lead to a better understanding of OA. However, further in vivo studies are needed to validate these methods in clinical practice.

We have developed a novel technique to analyse bone, using imaging mass cytometry (IMC) without the constraints of using immunofluorescent histochemistry. IMC can measure the expression of over 40 proteins simultaneously, without autofluorescence. We analysed mitochondrial respiratory chain (RC) protein deficiencies in human bone which are thought to contribute to osteoporosis with increasing age.

Osteoporosis is characterised by reduced bone mineral density (BMD) and fragility fractures. Humans accumulate mitochondrial mutations and RC deficiency with age and this has been linked to the changing phenotype in advancing age and age-related disease. Mitochondrial mutations are detectable from the age of 30 onwards, coincidently the age BMD begins to decline. Mitochondria contain their own genome which accumulates somatic variants at around 10 times the rate of nuclear DNA. Once these mutations exceed a threshold, RC deficiency and cellular dysfunction occur. The PolgD257A/D257A mouse model expresses a proof-reading deficient version of PolgA, a mtDNA polymerase. These mice accumulate mutations 3-5 times higher than wild-type mice showing enhanced levels of age-related osteoporosis and RC deficiency in osteoblasts.

Bone samples were analysed from young and old patients, developing a protocol and analysis framework for IMC in bone tissue sections to analyse osteoblasts in-situ for RC deficiency.

Samples from the femoral neck of 10 older healthy volunteers aged 40 – 85 were compared with samples from young patients aged 1-19. We have identified RC complex I defect in osteoblasts from 6 of the older volunteers, complex II defects in 2 of the older volunteers, complex IV defect in just 1 older volunteer, and complex V defect in 4 of the older volunteers.

These observations are consistent with the PolgD257A/D257A mouse-model and suggest that RC deficiency, due to age-related pathogenic mitochondrial DNA mutations, may play a significant role in the pathogenesis of human age-related osteoporosis.

Abstract

Total joint arthroplasty (TJA) is one of the commonest and most successful orthopaedic procedures used for the management of end-stage arthritis. With the recent introduction of robotic-assisted joint replacement, Computed tomography (CT) has become part of required pre-operative planning.

The aim of this study is to quantify and characterise incidental CT findings, their clinical significance, and their effect on the planned joint arthroplasty.

All consecutive patients undergoing an elective TJA (total joint arthroplasty; hip or knee) were retrospectively identified, over a 4-year period (December 2019 and November 2023). Data documented and analysed included patient demographics, type of joint arthroplasty, CT findings, their clinical significance, as well as potential delays to the planned arthroplasty because of these findings and subsequent further investigation.

A total of 987 patients (female: 514 patients (52.1%)) undergoing TJA were identified (THA: 444 patients (45.0%); TKA: 400 patients (40.5%); UKA: 143 patients (14.5%)). Incidental findings within imaged areas were identified in 227 patients (23.0%). Of these findings, 74 (7.5%) were significant, requiring further investigation or management, 40 (4.1%) of which represented potential malignancy and 4 (0.4%) resulting in a new cancer diagnosis. A single patient was found to have an aneurysm requiring urgent vascular intervention. Surgery was delayed for further investigation in 4 patients (0.4%). Significant findings were more frequent in THA patients (THA: 43 (9.7%) TKA/UKA: 31 (5.7%)

Within our cohort, 74 (7.5%) patients had significant incidental findings that required further investigations or management, with 4 (0.4%) having a previously undiagnosed malignancy. We strongly advocate that all robotic arthroplasty planning CTs are reviewed and reported by a specialist, to avoid missing undiagnosed malignancies and other significant diagnoses.

Imaging of the musculoskeletal system is vital for delivering optimum treatment particularly in the assessment of fracture healing. X-ray and CT are adequate imaging methods for bone but, soft tissue needs other modalities such as MRI and Ultrasound. We propose the use of Freehand 3D Ultrasound to study the early stages of fracture healing by imaging the bone surfaces around the fracture site and monitoring changes in the surrounding soft tissue. Freehand 3D ultrasound is acquired by attaching a position sensor to the probe of a conventional 2D diagnostic ultrasound machine. As the probe is moved, its position and orientation are recorded along with the 2D ultrasound images. This enables slices through the body to be viewed that would be inaccessible using a normal ultrasound system. Bone surfaces around a fracture site are scanned and the data reconstructed using the Stradx and Stradwin software developed by Cambridge University, to give a 3D visualization of the area. To assess the feasibility of this proposed method the lower limbs of healthy volunteers were scanned using a 5–10MHz ultrasound probe. The scanning resolution of the system was evaluated using a phantom to ensure millimetre detail could be detected as would be required for imaging early fracture healing. It was found that detail down to 0.8mm could easily be resolved for measurement. The 3D system could accurately profile the different soft tissue interfaces. The visible surfaces of the tibia were reconstructed to give 3D models. Additional layers of soft tissue interfaces could easily be added to these models to provide more detail. This imaging modality can provided detailed 3D models of bone the bone surface and surrounding soft tissue. As ultrasound is non-ionizing, rescanning can be conducted more frequently than with CT or x-ray thus offering a more accurate assessment of a patient’s response to healing

Introduction and Objective

Septic arthritis is an acute infective presentation of the joint calling for urgent intervention, thus making the differential diagnosis process difficult. An increase in temperature in the area containing the suspected septic arthritis is one of the clinically important findings. In this study, it was aimed to investigate whether or not the temperature changes obtained through thermal camera can be used as a new additional diagnostic tool in the differential diagnosis of septic arthritis.

There is a difference of opinion regarding the usefulness of MR Imaging as a diagnostic tool for triangular fibrocartilage complex (TFCC) tears in the wrist. Our aim was to determine the accuracy of direct magnetic resonance arthrography (MRA) in the diagnosis of triangular fibrocartilage complex (TFCC) tears of the wrist in a district general hospital setting. In a retrospective review of 21 patients who presented with complains of wrist pain and following a clinical examination, all had direct MR arthrography of the wrist in our hospital in a 1.5Tesla scanner. All had a diagnostic arthroscopy within 2-4 months of the MR scan. All patients had chronic ulnar sided wrist pain, although only two had a definite history of trauma. The findings of each diagnostic method were compared, with arthroscopy considered the gold standard. Twenty-one patients were studied (10 male: 11 female), mean age 42 years (range 27-71) years). Seventeen TFCC tears were diagnosed on arthroscopy. For the diagnosis of TFCC tears MRA had a sensitivity, specificity and accuracy of 67%. Our results echoed the opinion of some of the previous investigators with an unacceptable sensitivity or specificity for a diagnostic tool. MR arthrography needs to be further refined as a technique before it can be considered to be accurate enough to replace wrist arthroscopy for the diagnosis of TFCC tears. Other centres have reported better accuracy, using more advanced MRI technology. Until this iswidely available at all levels of healthcare the results of MRI for the diagnosis of TFCC tears should be interpreted with caution

There is no consensus regarding the optimum frequency of ultrasound for monitoring the response to Pavlik harness (PH) treatment in developmental dysplasia of hip (DDH). The purpose of our study was to determine if a limited-frequency hip ultrasound (USS) assessment in children undergoing PH treatment for DDH had an adverse effect on treatment outcomes when compared to traditional comprehensive ultrasound monitoring.

This study was a single-center non-inferiority randomized controlled trial. Children aged less than six months of age with dislocated, dislocatable and stable dysplastic hips undergoing a standardized treatment program with a PH were randomized, once stability had been achieved, to our current standard USS monitoring protocol (every clinic visit) or to a limited-frequency ultrasound protocol (USS only until hip stability and then end of treatment). Groups were compared based on alpha angle at the end of treatment, acetabular indices (AI) and IHDI grade on follow up radiographs at one-year post harness and complication rates. The premise was that if there were no differences in these outcomes, either protocol could be deemed safe and effective.

One hundred patients were recruited to the study; after exclusions, 42 patients completed the standard protocol (SP) and 36 completed the limited protocol (LP). There was no significant difference between the mean age between both groups at follow up x-ray (SP: 17.8 months; LP: 16.6 months; p=0.26). There was no difference between the groups in mean alpha angle at the end of treatment (SP: 69°; LP: 68.1°: p=0.25). There was no significant difference in the mean right AI at follow up (SP: 23.1°; LP: 22.0°; p=0.26), nor on the left (SP:23.3°; LP 22.8°; p=0.59). All hips in both groups were IHDI grade 1 at follow up. The only complication was one femoral nerve palsy in the SP group. In addition, the LP group underwent a 60% reduction in USS use once stable.

We found that once dysplastic or dislocated hips were reduced and stable on USS, a limited- frequency ultrasound protocol was not associated with an inferior complication or radiographic outcome profile compared to a standardized PH treatment pathway. Our study supports reducing the frequency of ultrasound assessment during PH treatment of hip dysplasia. Minimizing the need for expensive, time-consuming and in-person health care interventions is critical to reducing health care costs, improving patient experience and assists the move to remote care. Removing the need for USS assessment at every PH check will expand care to centers where USS is not routinely available and will facilitate the establishment of virtual care clinics where clinical examination may be performed remotely.

Summary

Objective assessment of tendon histomorphology, particularly in the context of tissue repair, requires comprehensive analyses of both cellular distribution and matrix architecture. Fourier Transform analyses of histological images collected with second harmonic generation (SHG-FT) technique provide objective, quantitative assessment of collagen fiber organization with high specificity. Concurrent nuclear staining allows simultaneous analyses of cell morphology and distribution.

Introduction: Whilst conventional Magnetic Resonance Imaging (MRI) is universally used as the method of choice for examining the boundaries of the intervertebral disc clinically it gives little information about the internal structure of the disc. This is largely due to the fact that the normal resolution of such devices (typically 1mm in plane and 3mm out of plane) is just too large to resolve structures and pathologies of interest. Aim: This work aims to describe the appearance of normal and pathological discs when imaged using a high resolution system. It then tests the hypothesis that a degeneration grading scale based upon such observations corresponds well with the graded appearance of the sectioned disc. Method: 13 lumbar discs from 7 non-chondrodystrophoid dogs (age 2–10 yr, mean 5.7 yr) were employed in this study. They were imaged using a small bore 0.5T research imaging system using a T2* weighted pulse sequence (TR=500ms, TE=17ms), a 60mm field of view, 1 mm slice thickness, in plane resolution was 230 μm. A grading scale based on the standard visual scale was developed for grading these images. Results: The outer and middle annulus had a strongly banded appearance with adjacent lamellae having high and low intensities (in spite of there similar chemical composition). The inner annulus (and frequently all the posterior annulus) had a uniform high intensity appearance as did the nucleus. Frequently, there has a well defined dark boundary between the annulus and nucleus. Increasing degeneration lead to disorder of the annulus structure and non-uniformity in the nucleus. Statistical comparison of the visual and MRI grading scales were extremely good (α=0.90–0.95) except for the posterior annulus (α=0.26). Conclusion: Many features of the MRI appearance of discs at high resolution, such as the banded structure of the annulus, were not expected and must be due to some subtle physical processes. Care must therefore be taken with the interpretation of such images, in particular to assessment of hydration. Grading of high resolution images corresponded well to the ‘gold standard’ of visual appearance on sectioning. However, this scale is totally different to that used to grade discs using conventional clinical MRI

Study Design: A retrospective analysis of patients with spinal disorders using Magnetic Resonance Imaging (MRI) results. Objective: To review the clinical and MRI results of patients with various scoliotic deformities. Background: Insufficient reports exist regarding the MRI’s of scoliotic deformities. MRI’s can offer vital information in the diagnosis of various types of scoliosis and their concomitant disorders. Methods: MRI reults of a total of 277 patients with various types of scoliosis/kyphoscoliosis were reviewed. All patients met the cobb angle criteria: > or = 20 degrees. 65 (23.46%) patients were male and 212 (76.53%) were female. 224 (80.86%) patients received conservative treatment and 53 (19.14%) underwent surgical treatments. 107 (38.62%) patients had adolescent idiopathic scoliosis, mean age: 13.7 (7–18) years, 76 (27.43%) adult idiopathic, mean age: 29.53 (19–79) years, 48 (17.32%) congenital, mean age: 12.6, (1–46) years, 29 (10.46%) neuromuscular, mean age: 12.86 (2–30) years, 15 (5.41%) syndromic, mean age: 13.6 (1–29) years, 2 (0.72%) tumor related, mean age:10.5 (8–13) years. Results: MRI results revealed the spinal cord of 169 (61.01%) patients as normal: no spinal cord anomalies, tumors, or congenital problems. Of the remaining patients 108 (38.98%) the following irregularities were diagnosed: 39 (36.11%) syringohydromyeli in various spinal locations, 29 (26.85%) butterfly vertebrae, 19 tethered cord (17.59%), 10 (9.2%) split cord, 10 diastometamyelia, 10 cleft vertebrae, 14 (12.96%) myelomeningocele, 7 (6.48%) grade one spondilolisthezis, 5 (4.62%) caudal regression syndromes, 6 (5.55%) vertebra partial fusion, 4 (3.7%) cranio-cervical problems, 4 cerebellar tonsillar ectopia, 3 (2.77%) block vertebra, 3 chiari typ2 II, 3 TIS, 2 (1.85%) tumors on the spinal column, 2 neurofibromatosis, 2 introdural lipoma, 2 myelomalacia of the spinal cord, 2 spinal cord injuries, 1 (0.92%) arachnoid cyst, 1 neuroanteric, 1 spina bifida, 1 scheuermann, 1 vertebral artery hypoplasia, 1 sacral dermal sinus, 1 cervical rib, 1 interpedicullar cyst, 1 high scapula, 1 sphenoid sinus retention cyst, 1 paravertebral cyst, 1 Schmorl’s node, 1 Tarlow cyst and 1 intercranial pineal cyst. Conclusion: Our study revealed how MRI analysis can lead to the accurate diagnosis of scoliotic deformities. In many cases tumors, neuromuscular pathology and syndromic conditions can be misdiagnosed as scoliotic. Careful MRI review can offer vital information for diagnosis and help determine the classification of scoliosis and subsequent treatment

Femoroacetabular impingement (FAI) results from a morphological deformity of the hip and is associated with osteoarthritis (OA). Increased bone mineral density (BMD) is observed in the antero-superior acetabulum rim where impingement occurs. It is hypothesized that the repeated abnormal contact leads to damage of the cartilage layer, but could also cause a bone remodelling response according to Wolff's Law. Thus the goal of this study was to assess the relationship between bone metabolic activity measured by PET and BMD measured in CT scans.

Five participants with asymptomatic cam deformity, three patients with uni-lateral symptomatic cam FAI and three healthy controls were scanned in a 3T PET-MRI scanner following injection with [18F]NaF. Bone remodelling activity was quantified with Standard Uptake Values (SUVs). SUVmax was analyzed in the antero-superior acetabular rim, femoral head and head-neck junction. In these same regions, BMD was calculated from CT scans using the calibration phantom included in the scan. The relationship between SUVmax and BMD from corresponding regions was assessed using the coefficient of determination (R²) from linear regression.

High bone activity was seen in the cam deformity and acetabular rim. SUVmax was negatively correlated with BMD in the antero-superior region of the acetabulum (R²=0.30, p=0.08). SUVmax was positively correlated with BMD in the antero-superior head-neck junction of the femur (R²=0.359, p=0.067). Correlations were weak in other regions.

Elevated bone turnover was seen in patients with a cam deformity but the relationship to BMD was moderate. This study demonstrates a pathomechanism of hip degeneration associated with FAI deformities, consistent with Wolff's law and the proposed mechanical cause of hip degeneration in FAI. [18F]-NaF PET SUV may be a biomarker of degeneration, especially in early stages of degeneration, when joint preservation surgery is likely to be the most successful.

Anatomic reduction and appropriate implant placement is essential for optimal treatment of intraarticular tibial plateau fractures. Standard intraoperative fluoroscopy provides limited visualization of the reduction and hardware placement compared with pre- or postoperative 3-D imaging modalities. As such, post-operative computer tomography (CT) has become a common procedure to evaluate the quality of the reduction and fixation. The Iso-C3D provides 3-D intraoperatively imaging to dynamically assess the surgical reduction and fixation at different anatomic regions. We report on our first 19 clinical tibial plateau fractures scanned intra-operatively with the Iso-C 3D. Between January and November 2003, 19 intraarticular tibia plateau fractures were scanned intraoperatively with the Iso-C3D (Siemens, Germany). No formal selection criteria were utilised except for the presence of a tibial plateau fracture. Operative procedures included 14 cases of open reduction internal fixation and 5 cases of internal fixation with arthroscopic assisted reduction. Imaging Technique: All patients were positioned on full-carbon tables for the operative procedure. After initial operative reduction and fixation, conventional two-dimensional fluoroscopic imaging was performed using standard AP and lateral projections. These images were evaluated by the operating surgeon; if the reduction and fixation was judged to be appropriate, Iso-C3D imaging was initiated. In 21% (n=4) of all cases an immediate revision of the operative procedure was performed after Iso-C3D imaging. These revisions were not deemed necessary with conventional fluoroscopy alone. In two cases, significant intra-articular incongruencies (greater than two millimetres) were noted. Additionally, in two cases, implant mal-position was detected. All patients had a postoperative CT scan. All CT scans confirmed the intraoperative Iso-C imaging, no further additional articular incongruencies or malpositioned implants were identified. When compared to conventional C-arm images, the Iso-C 3D scans demonstrated improved ability to identify the articular malreduction and implant mal-position in all cases. We have demonstrated that the Iso-C3D provides reliable intraoperative evaluation of reduction and hardware placement compared to traditional CT scans for tibial plateau fractures. In addition, clinically relevant intra-operative information was gained with its use in this study. In four (21%) cases, the operative treatment was modified due to the use of the multiplanar imaging modality. On average, 10 minutes of additional operative time was required for the use of Iso-C3D scanning and the evaluation of the images. Further prospective clinical studies are needed to improve our findings

Purpose of the study: To develop a new treatment algorithm for patients with chronic anterior knee pain based on kinematic patella tracking MR imaging. Methods and Results: Patients with anterior knee pain of more than one year duration and not responding to non-operative treatment, underwent kinematic MRI study. The provocative test was performed with the conventional MRI scanner and the patient extending the knee against resistance, the resistance provided by inflated beach ball. A retrospective analysis was done of first seventy patients, who had undergone this scanning technique. On the imaging films, four measurements were made. They were patella subluxation, tilt, cartilage thickness and the Tibial Tubercle Trochlear distance (TTD). Patellar subluxation was classified as mild, moderate and severe. We found that a Tibial Tubercle Trochlea distance of 18mm had a specifity of 100% and a sensitivity of 89% for severe maltracking. Conclusion: Kinematic MR Imaging is a useful investigation before considering operative treatment for patients with chronic anterior knee pain. Based on our study, we conclude that those patients with moderate lateral maltracking with a TTD< 18 mm should be offered lateral release and those with severe maltracking and TTD> 18mm should have a tibial tubercle transfer, in addition to lateral release

Surgical correction of spinal deformity in patients with neural axis abnormalities has established risks of causing further neurological injury. It is necessary to identify individuals with a predisposition for such abnormalities before treatment is instituted.

Objective: Examination of the association between idiopathic scoliosis and underlying neural axis abnormalities in the infantile and juvenile age groups.

Design: Retrospective chart and radiographic review.

Subjects: Ninety-four (36 infantile, 58 juvenile) consecutive patients with non-congenital scoliosis under the age of eleven years.

Outcome measures: These consisted of the MRI findings, neurological examination, associated curve morphology and necessity for neurosurgical intervention or surgical curve correction.

Results: Approximately 25% of patients presenting as idiopathic juvenile scoliosis had underlying neural axis abnormalities. No patient with apparent infantile idiopathic scoliosis had an abnormal spinal MRI scan. Using the Z score for independent proportions, there was a statistically significant difference between infantile and juvenile scoliosis and the presence of an underlying neural axis abnormality (Z score of 2.089, equivalent to p< 0.02).

Conclusions: We advocate routine MR spinal imaging in all patients with juvenile idiopathic scoliosis. In infantile idiopathic scoliosis, to avoid unnecessary general anaesthetics, one should image the spinal canal only when clinically indicated

Purpose of the study: To develop a new treatment algorithm for patients with chronic anterior knee pain based on kinematic patella tracking MR imaging. Methods and Results: Patients with anterior knee pain of more than one year duration and not responding to non-operative treatment, underwent kinematic MRI study. The provocative test was performed with the conventional MRI scanner and the patient extending the knee against resistance, the resistance provided by inflated beach ball. A retrospective analysis was done of first seventy patients, who had undergone this scanning technique. On the imaging films, four measurements were made. They were patella subluxation, tilt, cartilage thickness and the Tibial Tubercle Trochlear distance (TTD). Patellar subluxation was classified as mild, moderate and severe. We found that a Tibial Tubercle Trochlea distance of 18mm had a specifity of 100% and a sensitivity of 89% for severe maltracking. Conclusion: Kinematic MR Imaging is a useful investigation before considering operative treatment for patients with chronic anterior knee pain. Based on our study, we conclude that those patients with moderate lateral maltracking with a TTD< 18 mm should be offered lateral release and those with severe maltracking and TTD> 18mm should have a tibial tubercle transfer, in addition to lateral release

Introduction and Aims: Ganglia are commonly seen during investigation of patients with wrist pain. Our aim was to determine the prevalence of ganglia in an asymptomatic population. Method: Following Institutional Ethical approval, Magnetic Resonance Imaging (MRI) was performed on the wrists of 103 asymptomatic volunteers. There were 67 males and 37 females, with an average age of 36, range 19–67 years. There were 52 right wrists and 51 left wrists. Using a 1.89 Tesla surface coil Magnetic Resonance Imager the following sequences were obtained: Coronal T 1, Proton Density, T 2 and Inversion Recovery sequences; Sagittal Inversion Recovery sequences; Axial T 1 and Inversion Recovery Sequences. The images were then evaluated independently by two Muskuloskeletal Radiologists and one Orthopaedic Surgeon. Results: Wrist Ganglia were identified in 53 out of 103 wrists. Wrist Ganglia were more prevalent in females than males, 58% compared to 48% respectively. The average long axis measurement was 7.5 mm (range 2.7–22.2), the average short axis measurement was 3.2 mm (range 1.6–10.1). Seventy percent of the Ganglia were found to originate from the volar capsule in the region of the interval between the Radio-Scapho-Capitate Ligament and the Long Radio-Lunate Ligament. Fourteen percent of the ganglia were dorsal and originated from the dorsal, distal fibres of the Scapho-Lunate Ligament. Two ganglia had surrounding soft tissue oedema and one had an associated intra-osseous component. Conclusion: The prevalence of asymptomatic wrist ganglia is high – 51%. Unlike previous surgical and pathological series, our study showed volar wrist ganglia are more common than dorsal wrist ganglia in the asymptomatic population. The vast majority of these asymptomatic ganglia do not show associated ligamentous disruption, soft tissue oedema or intra-osseous communication

Introduction. The safe zone of the acetabular cup for THA was discussed based on the AP X-ray films of hip joints. A supine position is still used to determine the cup position for CAOS such as navigation systems. There were few data about the implant positions after THA in standing positions. The EOS X-Ray Imaging Acquisition System (EOS system) (EOS imaging Inc, Paris, France) allows image acquisition with the patients in a standing or sitting position. We can obtain AP and lateral X-ray images with high-quality resolution and low dose radiation exposure. Recently, we have obtained the EOS system for the first time in Japan. We investigated 3D accuracy of the EOS system for implant measurements after THA. Patients and Methods. We measured the implant angles of the 68 patients (59 females and 9 males, average age: 61y.o.) who underwent THA using the EOS system. The cup inclination and anteversion were measured in the anterior pelvic plane (APP) coordinate. The femoral stem antetorsion was defined as angles between the stem neck axis and the posterior condylar axis. These data were compared with the implant angles of the same patients measured by the post-operative CT scan images and the 3D image analysis using the ZedHip software (LEXI, Japan). Results. The cup inclinations (average ±SE) measured by the EOS system and the CT scan were 40.6 ± 0.64° and 42.9 ± 0.53°, respectively. The cup anteversions were 22.9 ± 1.3° and 22.8 ± 1.0°, respectively. The stem antetorsions were 28.9 ± 1.3° and 29.8 ± 1.6°, respectively. The differences (average ± SE) between the EOS system and the CT scan in the cup inclination, the cup anteversion, and the stem antetorsion were −2.3 ± 0.38°, −0.09 ± 0.82°, and −0.90 ± 0.91°, respectively. There were strong correlations in measurement values between the EOS system and the CT scan (the Spearman's correlation coefficients of the cup inclination, the cup anteversion, and the stem antetorsion were 0.6521 [p<0.001], 0.7154 [p<0.001], and 0.8645 [p<0.001], respectively). Discussion. The EOS system provides acceptable clinical accuracies in measuring acetabular cup and femoral stem angles after THA. The accuracy of the cup angles was accorded with that of the basic experimental data using a dry pelvis. Our data also demonstrated clinically acceptable accuracy in the measurement of stem antetorsion. This system can provide accurate snap shots of variable postures with high resolution. Using the EOS system, we may establish real optimum positions of THA implants by measuring the patients after THA in several postures including standing, squatting or sitting positions which required for Japanese ADL

Background

Magnetic resonance imaging (MRI) algorithm identifies end stage severely degenerated disc as ‘black’, and a moderately degenerate to non-degenerated disc as ‘white’. MRI is based on signal intensity changes that identifies loss of proteoglycans, water, and general radial bulging but lacks association with microscopic features such as fissure, endplate damage, persistent inflammatory catabolism that facilitates proteoglycan loss leading to ultimate collapse of annulus with neo-innervation and vascularization, as an indicator of pain. Thus, we propose a novel machine learning based imaging tool that combines quantifiable microscopic histopathological features with macroscopic signal intensities changes for hybrid assessment of disc degeneration.

Pelvic osteotomies for hip dysplasia results can be variable and depend on the amount of preexisting arthritis. Delayed gadolinium-enhanced magnetic resonance imaging of cartilage (dGEMRIC) is a technique designed to measure early arthritis, and could be used to select hips that would benefit from a joint-preserving reconstructive procedure. Our objective was to investigate the role of preoperative dGEMRIC in predicting the success of PAO in patients 40 and above. We hypothesized that patients who failed had lower preoperative dGEMRIC index compared to those who did not.

Following IRB approval, patients 40 or older who underwent PAO between 1990–2013 and had preoperative dGEMRIC scan and minimum follow-up of 4 years were identified. Patients with prior hip surgeries or any pathologies were removed leading to a total of 70 patients (Age: 44.2 ± 2.9 years old, BMI: 25.7 ± 4.5 Kg/m²). We only included the first hip undergoing PAO for those with bilateral PAO. Out of 70, 19 had failure defined by the need for total hip replacement or WOMAC pain score of 10 and above within 10 years after index PAO surgery. Articular cartilage was segmented on the 3D pre-operative dGEMRIC scan. The average thickness and dGEMRIC index across the whole articular surface were analyzed.

Failed hips had a lower dGEMRIC index by 115 ± 20 ms (P<0.001). All but one failed hips had a dGMERIC index of 400 or less (range: 313 – 479 ms), while all survived hips had a dGMERIC index of greater than 400 (range: 403 – 691 ms). Similar trends were observed when comparing the dGEMRIC index within the 6 subgroups (P<0.01). There were no differences in cartilage thickness (combined femoral head and acetabular cartilage) between the failed and survived hips (p>0.2).

Patients with a high dGMERIC index (indicating high GAG content) may have a higher chance of successful outcomes following PAO. Current efforts are underway to develop a multi-modal predictive model to evaluate risk of failure after PAO.

Introduction. In degenerative lumbar spine, it seems possible that foraminal stenosis is over-diagnosed as axial scanning is not performed in the plane of the exiting nerve root. We carried out a two-part study to determine the true incidence of foraminal stenosis. Patients and Methods. Initially we performed a retrospective analysis of radiology reports of conventional Magnetic Resonance Imaging in 100 cases of definite spinal stenosis to determine the incidence of reported ‘foraminal stenosis’. Subsiquently we performed a prospective study of MRI including fine slice T2 and T2 STIR coronal sequences in 100 patients with suspected stenosis. Three surgeons and one radiologist independently compared the diagnoses on conventional axial and sagittal sequences with the coronal scans. Results. The retrospective analysis found that ‘foraminal stenosis’ was reported by radiologists in 46% using conventional axial and sagittal sequences. In the prospective study of 100 patients suspected of having stenosis, spinal stenosis was reported in 40; degenerative spondylolisthesis in 14; posterolateral disc herniation in 14; normal report in 13; far lateral disc herniation in 7; isthmic (lytic) spondylolisthesis in 6; and degenerative scoliosis in 6. Conventional sequences diagnosed lateral recess stenosis reliably, but also suggested foraminal stenosis in 43%. However, coronal sequences clearly showed no foraminal nerve compression at all. In degenerative spondylolisthesis conventional scans suggested foraminal stenosis in 10 of 14 cases. Coronal imaging again showed no foraminal stenosis. Excellent correlation was found in normal spines and in disc herniation. Foraminal nerve compression was confirmed by conventional and coronal imaging only in isthmic spondylolisthesis, degenerative scoliosis and far lateral disc herniation. Conclusion. The addition of coronal MRI proves that foraminal stenosis is over-diagnosed. True foraminal stenosis definitely exists in isthmic spondylolisthesis, degenerative scoliosis and far lateral disc herniation, but we question its existence in spinal stenosis and degenerative spondylolisthesis

Background

The advent of EOS imaging has offered clinicians the opportunity to image the whole skeleton in the anatomical standing position with a smaller radiation dose than standard spine roentgenograms. It is known as the fifth modality of imaging. Current NICE guidelines do not recommend EOS scans over x-rays citing: “The evidence indicated insufficient patient benefit in terms of radiation dose reduction and increased throughput to justify its cost”.

Summary Statement. In this study, we observed that MR16-1, an interleukin-6 inhibitor, recovered phosphatidylcholine containing docosahexaenoic acid at the injury site after spinal cord injury in mice model by using imaging mass spectrometry. Introduction. The current drugs for improving motor function of the limbs lost due to spinal cord injury (SCI) are ineffective. Development of new drugs for spinal cord injury is desired. MR16-1, an interleukin-6 inhibitor, is found to be effective in improving motor function after spinal cord injury in mice model. Thus, we examined the molecular mechanism in more detail. Therefore, the purpose of this study was to analyze the molecular changes in the spinal cord of the SCI mice treated with MR16-1 using imaging mass spectrometry. Methods. All experiments were performed according to the guidelines for animal experimentation and care and use of laboratory animals established by Hamamatsu University School of Medicine (Shizuoka, Japan). We used 36 adult female C57BL/6J mice for laminectomy and contusion injury of the spinal cord that were performed at the T10 level using the Infinite Horizon Impactor (IH Impactor, 60 kdyn; Muromachi, Tokyo, Japan). Immediately after SCI, mice were intraperitoneally injected with a single dose of MR16-1 (Chugai, Tokyo Japan) (100 µg/g body weight, MR16-1 group) or a single dose of phosphate-buffered saline (PBS) of the same volume (control group). Motor function of the hind limbs was evaluated using the Basso Mouse Scale (BMS), an open-field locomotor test in which the scores range from 0 points (scored for no ankle movement) to 9 points (scored for complete functional recovery). BMS scores were recorded at 1, 7, 14, 21, 28, 35, and 42 days after SCI. The spinal cord tissues were flash frozen and were sliced to a thickness of 8 µm using a cryostat (CM1950; Leica, Wetzler, Germany). Imaging mass spectrometry was used to visualise 12 molecular species of phosphatidylcholine (PC) from thin slices of the spinal cords obtained at 7 days post-SCI. Results. The contusive SCI immediately resulted in complete paralysis. The MR16-1–treated group showed a significant improvement in the BMS locomotor score compared with the control group at both 7 days and 42 days after SCI (1.4 vs 0.2 points and 4.0 vs 1.4 points, respectively). Phospholipids at 7 days after SCI showed unique distribution patterns. In particular, PCs containing docosahexaenoic acid (DHA) localised in the gray matter region was significantly higher in the MR16-1–treated group than in the control group, at 7 days post-SCI. Discussion. MR16-1 treatment showed to improve locomotor BMS score after 7 days of SCI compared with that observed in the control group. Spinal cord injury had induced inflammation; injury sites showed changes in the lipid content. We had previously reported that PC containing DHA mostly expressed in neuron cells decrease on injury sites. In this study, we observed that MR16-1 recovered PC containing DHA at the injury site. This may be associated with the recovery of motor function