Introduction. Growing evidence in the literature suggests better clinical and functional outcomes and lower re-rupture rates with repair compared to non-operative treatment of ruptured Achilles tendon. There are however, concerns of wound infection, nerve injury and scar tenderness with the standard open and percutaneous techniques of repair. We aim to evaluate clinical and functional outcomes and complications in patients treated with minimally invasive Achillon device. Materials and Methods. Prospectively collected clinical data was reviewed of all consecutive patients who underwent repair of the ruptured Achilles tendon using the Achillon device. Patients were contacted using a postal questionnaire for assessment of their functional status using the validated Achilles Tendon Total Rupture Score (ATRS) and compared with their uninjured side. The outcomes were compared to the published results. Results. We present patient demographics; and clinical and functional outcomes of 140 consecutive patients who underwent repair of ruptured Achilles tendon using the minimally invasive technique between June 2007 to August 2010. Our complication rate was of two-three percent each of proximal DVT, scar sensitivity, superficial wound infection and no re-rupture after a minimum of 6 months. There was no case of nerve injury leading to permanent neurological deficit. Discussion. This study demonstrates good clinical and functional outcomes from using the Achillon device in repair of ruptured Achilles tendon with a low complication rate comparable to other published series. We recommend Achillon device as a safe minimally invasive technique for repair of the ruptured Achilles tendon

The Achilles tendon is the strongest and largest tendon in the body. Rupture of this tendon usually occurs in the third and fourth decade and can be significantly debilitating. Repair of neglected ruptures of Achilles tendon pose a challenge to the orthopaedic surgeon due to the retraction and atrophy of the ends of the tendon. Various surgical procedures have been described which include VY plasty, fascia lata, peroneus brevis, plantaris tendon, flexor digitorum longus, flexor hallucis longus, allograft, and synthetic materials. We carried out a prospective study to compare the results of peroneus brevis transfer with flexor hallucis longus transfer in the management of neglected ruptures of Achilles tendon. Forty-seven patients who had neglected ruptures of Achilles tendon were included in this study. They were randomly divided into two groups; the first group underwent peroneus brevis transfer (24 patients) and the second group had flexor hallucis longus transfer (23 patients). Patients were assessed using the Quigley's scoring system. We conclude that Flexor hallucis longus transfer is better than peroneus brevis transfer as it is a long, durable tendon which is much stronger when compared to other tendon transfers. Flexor hallucis longus acts in the same axis as the Achilles tendon, is in the same gait phase and is in close proximity, making harvesting of the tendon easy

We hypothesised that a minimally invasive peroneus brevis tendon transfer would be effective for the management of a chronic rupture of the Achilles tendon. In 17 patients (three women, 14 men) who underwent minimally invasive transfer and tenodesis of the peroneus brevis to the calcaneum, at a mean follow-up of 4.6 years (2 to 7) the modified Achilles tendon total rupture score (ATRS) was recorded and the maximum circumference of the calf of the operated and contralateral limbs was measured. The strength of isometric plantar flexion of the gastrocsoleus complex and of eversion of the ankle were measured bilaterally. Functional outcomes were classified according to the four-point Boyden scale. . At the latest review, the mean maximum circumference of the calf of the operated limb was not significantly different from the pre-operative mean value, (41.4 cm, 32 to 50 vs 40.6 cm, 33 to 46; p = 0.45), and not significantly less than that of the contralateral limb (43.1 cm, 35 to 52; p = 0.16). The mean peak torque (244.6 N, 125 to 367) and the strength of eversion of the operated ankle (149.1 N, 65 to 240) were significantly lower (p < 0.01) than those of the contralateral limb (mean peak torque 289, 145 to 419; strength of eversion: 175.2, 71 to 280). The mean ATRS significantly improved from 58 pre-operatively (35 to 68) to 91 (75 to 97; 95% confidence interval 85.3 to 93.2) at the time of final review. Of 13 patients who practised sport at the time of injury, ten still undertook recreational activities. . This procedure may be safely performed, is minimally invasive, and allows most patients to return to pre-injury sport and daily activities. Cite this article: Bone Joint J 2015;97-B:353–7

A total of seven patients (six men and one woman) with a defect in the Achilles tendon and overlying soft tissue underwent reconstruction using either a composite radial forearm flap (n = 3) or an anterolateral thigh flap (n = 4). The Achilles tendons were reconstructed using chimeric palmaris longus (n = 2) or tensor fascia lata (n = 2) flaps or transfer of the flexor hallucis longus tendon (n = 3). Surgical parameters such as the rate of complications and the time between the initial repair and flap surgery were analysed. Function was measured objectively by recording the circumference of the calf, the isometric strength of the plantar flexors and the range of movement of the ankle. The Achilles tendon Total Rupture Score (ATRS) questionnaire was used as a patient-reported outcome measure. Most patients had undergone several previous operations to the Achilles tendon prior to flap surgery. The mean time to flap surgery was 14.3 months (2.1 to 40.7).

At a mean follow-up of 32.3 months (12.1 to 59.6) the circumference of the calf on the operated lower limb was reduced by a mean of 1.9 cm (sd 0.74) compared with the contralateral limb (p = 0.042). The mean strength of the plantar flexors on the operated lower limb was reduced to 88.9% of that of the contralateral limb (p = 0.043). There was no significant difference in the range of movement between the two sides (p = 0.317). The mean ATRS score was 72 points (sd 20.0). One patient who had an initial successful reconstruction developed a skin defect of the composite flap 12 months after free flap surgery and this resulted in recurrent infections, culminating in transtibial amputation 44 months after reconstruction.

These otherwise indicate that reconstruction of the Achilles tendon combined with flap cover results in a successful and functional reconstruction.

Cite this article: Bone Joint J 2015;97-B:215–20.

Percutaneous repair of a ruptured Achilles tendon has been shown to reduce wound healing problems but it has a high incidence of injury to the sural nerve. The Achillon Suture System is a new method utilising a small longitudinal incision. It passes a suture through the Achilles tendon leaving the suture purely within the tendon. The aim of this prospective study was to investigate the results of a new mini-open technique utilising a horizontal incision and early active mobilisation. Following ethical committee approval 25 patients underwent repair of their ruptured Achilles tendon using the Achillon System. Rather than the longitudinal incision we used a horizontal incision and an accelerated rehabilitation program with a brace for six weeks post-operatively. Patients were followed up at six weeks, three and six months and one year post-op using the AOFAS and Leppilahti scoring systems. There were no wound complications, re-ruptures or sural nerve injuries. All patients returned to work or their previous daily activities by six weeks (mean 22 days) post op. All patients had returned to driving by six weeks. One patient had 10° restriction in dorsiflexion at three months, which prevented her return to running. She was back to running and had a full range of movement at six months. All other patients returned to sporting activities at three months but jumping sports such as basketball were discouraged until six months post-op. We suggest that this modification of using a horizontal incision and early mobilisation enhances wound healing and allows early return to normal activities and sports. It is technically simple, utilises a small incision (still enabling visual confirmation that the tendon ends have been approximated) and reduces the risk of sural nerve injury seen in other mini-open or percutaneous techniques

Percutaneous repair of a ruptured Achilles tendon has been shown to reduce wound healing problems but it has a high incidence of injury to the sural nerve. The Achillon Suture System is a new method utilising a small longitudinal incision. It passes a suture through the Achilles tendon leaving the suture purely within the tendon. The aim of this prospective study was to investigate the results of a new mini-open technique utilising a horizontal incision and early active mobilisation. Following ethical committee approval 25 patients underwent repair of their ruptured Achilles tendon using the Achillon System. Rather than the longitudinal incision we used a horizontal incision and an accelerated rehabilitation program with a brace for 6 weeks post-operatively. Patients were followed up at 6 weeks, 3 months and 6 months post-op using the AOFAS and Leppilahti scoring systems. There were no wound complications, re-ruptures or sural nerve injuries. All patients returned to work or their previous daily activities by 6 weeks (mean 22 days) post op. All patients had returned to driving by 6 weeks. One patient had 10° restriction in dorsiflexion at 3 months which prevented her return to running. She was back to running and had a full range of movement at 6 months. All other patients returned to sporting activities at 3 months but jumping sports such as basketball were discouraged until 6 months post-op. We suggest that this modification of using a horizontal incision and early mobilisation enhances wound healing and allows early return to normal activities and sports. It is technically simple, utilises a small incision (still enabling visual confirmation that the tendon ends have been approximated) and reduces the risk of sural nerve injury seen in other mini-open or percutaneous techniques

Introduction: The treatment of the recently ruptured Achilles tendon is still controversial. Surgical procedures are commonly considered to restore excellent functional capacity and suffer low re-rupture rates, but are potentially associated with significant complications like wound infection and paraesthesia. Recent studies report very good results with a conservative treatment in rigid casts. Our aim was to evaluate a new method of functional and conservative treatment using immobilisation in an articulated cast. Patients and Methods: Between March 1998 and August 2005, 12 women and 45 men with an average age of 45 (24–73), underwent a functional and conservative treatment for a recently ruptured Achilles tendon. After an immobilisation with a cast in equinus for 10 days, the patients were authorised to walk with full weight-bearing, protected by a commercial orthosis (VACO®ped, OPED). The equinus angle was set at 30° plantar flexion until the end of week 3, and at 15° until the end of week 4. At the 5th week the system was unlocked to allow ankle mobilisation of 30-15-0°, and at 30-0-0° at the seventh week. The orthosis was removed after 8 weeks. All patients had follow-up examinations up to 12 months after the trauma. The first 30 patients underwent a clinical examination and muscular testing with a Cybex isokinetic dynamometer at 6 and 12 months. In June 2006 all 57 patients were contacted and received a questionnaire. Their subjective opinions of the outcome, any change in their sport activities and eventual late complications were investigated. We evaluated the questionnaire and medical records using a scoring system based on the Leppilahti Ankle Score. Results: After one year there was not any difference in the motion of the ankle in comparison with the healthy side. There was no substantial calf amyotrophy and we found very little difference in muscular capacity. The average overall satisfaction with the outcome was 8.1 out of 10. We observed 5 complete re-ruptures (9%), 2 partial re-ruptures and 1 deep venous thrombosis complicated by pulmonary embolism. We observed few minor skin complications. Conclusions: The present treatment resulted in good to excellent functional results in most of the cases. It requires an active participation of the patient and a systematic medical follow-up during the first 6 months. The complication rate is acceptable. We think that early ankle mobilisation in the dynamic cast promotes better functional results than a rigid immobilisation technique. There is a place for conservative functional treatment in the acute rupture of the Achilles tendon. But prospective comparison with modern surgical techniques, like minimal invasive suture, is still required, especially in patients with high functional demand

We describe three patients in whom the tibial nerve was used, in mistake for the plantaris tendon, to repair a ruptured calcaneal tendon. The tendon repair was successful in all cases, but despite attempted reconstruction of the nerve, no patient had any motor recovery although two regained some protective sensation

Thirteen patients with ruptures of the calcaneal tendon diagnosed more than four weeks after injury were reviewed. Eleven patients had operative reconstruction with tendon shortening and the postoperative follow-up ranged from one to seven years. Isometric and isokinetic measurements, as well as the strength of the triceps surae, all compared favourably with the normal contralateral leg. Only one tendon re-ruptured. Eight of the eleven patients were satisfied with the results and the two patients who had refused reconstruction had worse functional results. Late reconstruction of a ruptured calcaneal tendon is thus a worthwhile procedure

Complete rupture of the Achilles tendon is a well described injury. Assessment of outcome and rehabilitation post surgery is difficult to qualify objectively. We undertook a prospective evaluation of patients who had surgical repair of their ruptured Achilles tendon at our institution over a five year period. All patients underwent clinical and functional assessment. Objective function was evaluated using isokinetic testing on a Biodex dynamometer. Measurements included peak torque/body weight, average power, deficits, total work and range of movement. Studies were repeated at 60, 120 and 240 degrees/second. This was correlated with average time off work and time of return and level of sporting activities. Although 53 patients were entered into the study, only 24 returned for full isokinetic testing. Males (16) were more commonly affected than females (9). Peak incidence was in the fourth and fifth decades of life. Mean hospital stay was 1.8 days. There were no problems with wound healing, and there were no infections. 22 of 25 patients returned to their pre-injury level of activity after an average of 6 months. Time off work averaged 14 weeks. 16 of 25 patients were the same or better when comparing peak torque/body weight, average power, maximum average peak torque and total work/body weight when compared with the unaffected limb. Rehabilitation following Achilles tendon repair can be monitored clinically, however isokinetic testing can provide a more objective assessment of progress

Using a comparative, longitudinal study design, we studied the effects of early weight bearing and ankle mobilisation following acute repair of ruptured Achilles tendon. Patients in Group 1 (22 males and 4 females; mean age 44.7 years [range 31–69], 11 right- and 15 left-sided ruptures) were immobilised with their ankle in gravity equinus, and encouraged to fully weight bear. They received a single cast change at 2 weeks, when the ankle was accommodated in an anterior splint, allowing full plantarflexion but not dorsiflexion above neutral. Patients in Group 2 (23 males and 4 females; mean age 43.8 years [range 30–67], 11 right- and 16 left-sided ruptures) were immobilised in full equinus. They received a cast change at 2 and 4 weeks, when the ankle was immobilised in a plantigrade position. They were advised to weight bear 4 weeks after the operation. Patients in Group 1 attended less outpatient visits and completely discarded their crutches at an average of 2.5 weeks after the operation. Group 2 discarded their crutches at an average of 5.7 weeks after from the operation (p=0.013). At ultrasound scan, the average thickness of the repaired tendon was 12.1 mm (SD 2), with no difference in the thickness of the ruptured tendon regardless of the method of post-operative management. There was no significant difference in isometric strength between the two groups of patients. A greater proportion of patients in Group 1 were satisfied with the results of surgery (p=0.04). Early weight bearing with the ankle plantigrade is not detrimental to the outcome of repair following rupture of the Achilles tendon, and shortens the time needed for rehabilitation. However, strength deficit and muscle atrophy are not prevented

Significant variation exists amongst surgeons regarding the post operative rehabilitation protocol after surgical repair of Achilles tendon ruptures. The objective of this study was to determine the effect of early weight bearing on the postoperative recovery after surgical repair of ruptured Achilles tendons. The hypothesis was that an early weight bearing routine does not increase the rate of re-rupture or other complications, and improves the quality of life by reducing discomfort of the rehabilitation phase and allowing an earlier return to work and sport. A prospective, randomised controlled trial of patients with Achilles tendon ruptures undergoing surgical repair was performed over a three year period concluding in April 2006. Specific inclusion and exclusion criteria were used to include one hundred and ten patients in the study at two centers involving twelve surgeons. Patients were randomised to either weight bearing or non weight bearing at the initial postoperative visit and compliance was monitored with a pressure sensor in the fixed-hinged ankle foot orthosis. Patients were assessed at six, twelve and twenty-six weeks postoperatively for 1) re-rupture rate, 2) strength of calf musculature 3) ankle range of motion, 4) complications, 5) return to sporting activities, and 6) return to work. In addition, health–related quality of life was measured using the SF-36 and AOFAS Ankle-Hindfoot Scale. Enrollment was completed as of May 2006 and six month data is over 90% completed. We are on course for < 10% loss to follow-up. Thus far, there have been no re-ruptures in either group. Definitive data analysis can only be performed once all one hundred and ten patients have completed their six month follow up. Once data analysis is completed, comparisons of patient satisfaction, strength, range of motion, complications, return to sport and return to work will be discussed. Early weight bearing after Achilles tendon repair did not increase the rate of re-rupture. Final data analysis will document any significant differences in the parameters measured. We believe this study will provide a definitive answer on the safety of early weight bearing after surgical repair of Achilles tendon ruptures

The current standard of care in Calgary, Alberta for management of a ruptured Achilles tendon is surgical repair, typically performed following admission to hospital. The primary objective of this study was to compare the costs of hospital treatment and complications associated with the surgical repair of Achilles tendon ruptures between two groups of patients: Group One = patients enrolled in the randomised clinical trial (RCT) Multicentre Achilles Tendon Treatment Study (MATTS), Group Two = all other non-study patients. This observational cohort study analyzed all patients surgically treated for Achilles tendon ruptures at Calgary area hospitals over a three-year period (October 2002–September 2005). Inclusion criteria: age eighteen to seventy years, acute rupture. A total of two hundred and eighty-two patients met the inclusion criteria; thirty-three patients were included in Group One, two hundred and forty-nine patients in Group Two. In Group One, twenty-seven patients (82%) were treated as outpatients, five patients (15%) were ADOP-24hr, and one patient (3%) was admitted. In Group Two, twenty-seven patients (11%) were treated as outpatients, ninety-five patients (38%) were ADOP-24hr, and one hundred and twenty-seven patients (51%) were admitted. The total costs for patients treated as outpatients and requiring overnight stays in Group One were $18,408 and $7,419, respectively. In Group Two, the total cost for outpatients was $18,071 compared to $379,496 for non-study patients requiring overnight stay. If all overnight patients in each group were treated as outpatients, the total savings would be $235,545. There were no serious complications in Group One. In Group Two, complications included two cases of pulmonary embolus, and one case of compartment syndrome requiring readmission. All complications resolved. Surgical treatment of Achilles tendon rupture can be performed safely and at less cost on an outpatient basis. Participation in this RCT has allowed us to recommend a change in the standard of care in Calgary

Introduction: The diagnosis of Achilles tendon rupture must be made promptly and reliably to prevent avoidable morbidity. The calf squeeze test (CST) offers a simple clinical test with high sensitivity. However, in our clinical practice we have noticed a lack of clarity in the medical notes. We believe there is confusion regarding what constitutes a positive CST. Movement of the foot being positive or lack of movement of the foot indicating the test is positive. The purpose of this study was to assess the degree of error and to determine whether this is due to lack of knowledge, an inability to perform or correctly interpret and record the result of the CST. We assessed SHO’s , Registrars and Consultants in the Accident & Emergency and Orthopaedic Departments. Method: Ninety one doctors completed a supervised questionaire. They were asked four questions 1) What tests they chose to diagnose Achilles tendon rupture. 2) How they would perform a CST. 3) What they considered a positive CST to mean . 4) How they would record the diagnosis of a ruptured Achilles tendon. Results: 92%(84/91) of doctors overall chose to use a CST. 88%(80/91) performed the CST correctly. The CST was interpreted incorrectly by 41%(15/37) and 26%(14/54) of A + E and Orthopaedic doctors respectively. Also 32%(12/37) of A + E and 19%(10/54) of Orthopaedic department doctors mistakenly thought that the diagnosis of an Achilles tendon rupture was consistent with a negative test. Conclusion: The results suggest that the recording of AT rupture may be inaccurate in as many as 32%(12/37) when patients present to the A + E department. This error is not a result of lack of knowledge or performance of the CST but of interpretation and recording. We would strongly discourage the recording of the CST in terms of a positive or negative result. The result should be described in words, for example ‘No movement of the foot on squeezing the calf muscle.’ We suggest that all SHOs and Registrars who may be called upon to assess patients with suspected Achilles tendon rupture are informed of this source of error

Introduction: Treatment for ruptured Achilles tendon can be classified into operative (open or percutaneous) and non-operative (cast immobilisation or functional bracing); post-operative splintage can be with a rigid cast or functional brace. The aim was to identify and summarise the evidence from randomised trials of the effectiveness of different interventions. Methods: We searched the Cochrane specialised register, MEDLINE, reference lists of articles and contacted trialists directly for all randomised and quasiran-domised trials comparing different treatment regimes for acute Achilles tendon ruptures. Results: Fourteen trials involving 891 patients were included. Open operative treatment compared with non-operative treatment was associated with a lower risk of re-rupture (odds ratio (OR) = 0.25, 95% confidence interval (CI) = 0.1–0.6, p=0.003) but a higher risk of other complications including infection, adhesions and disturbed sensibility (OR = 14.1, 95%CI = 6.3–31.7, p< 0.00001). Open versus percutaneous operative surgical repair was associated with a longer operation duration and higher risk of infection (OR = 12.9, 95%CI = 1.6–105.6, p=0.02). Patients splinted with a functional brace rather than a cast post-operatively tended to have a shorter in-patient stay, less time off work, quicker return to sporting activities and fewer reported complications (p=0.0003). Because of the small number of patients involved no definitive conclusions could be made regarding different operative techniques and different non-operative regimes. Conclusions: Open operative treatment significantly reduces the risk of re-rupture but has the drawback of a significantly higher risk of other complications, including wound infection. The latter may be reduced by performing surgery percutaneously. Post-operative splintage in a functional brace appears to reduce hospital stay and time off work and sports

Introduction: We studied prospectively two groups of patients treated operatively for acute achilles tendon rupture admitted in our institution in order to compare post operative morbidity, usage of hospital resources and immobilisation regimen involving immediate weight-bearing with traditional non-weight-bearing. The aim was to assess the benefit of instrument guided surgical method, which reduces hospitalisation cost, post operative wound care cost and reduce requirement of post-operative analgesics together with improved rehabilitation and return to normal activity for young patients [age below 45] with a rupture of the Achilles tendon. Material and methods: 34 patients had repair of the tendon with an open method as an inpatient under instruction of the admitting consultant. Second group of 25 patients had repair as limited open technique with an Achillon instrument and immediate weight bearing. Result: Opiates or opiate-based analgesia were used in the open repair group and in the minimally invasive group, patients reported no pain with paracetamol or ibuprofen. Two cases of severe wound infection leading to dehiscence requiring further surgery and 5 cases of minor surgical site infection leading to delayed wound healing were reported as wound complications in the open group. All the patients in the mini invasive group reported their satisfaction with wound healing and minimal scar at the incision site. Based on self-reports, the time taken to return to normal walking was median of 11 weeks in the achillon treatment group and 17 weeks for the open group. There was also an earlier return to normal stair climbing, with a median of 13 weeks [9–21 weeks] in the achillon treatment group and 19 weeks [13–27 weeks] for the opens technique. Conclusion: This study has shown that mini invasive repair with Achillon instrument may allow us to perform surgery with less bed usage, less consumption of post operative analgesics and other associated indirect cost to the healthcare provider. It also allows faster rehabilitation. It provides further evidence that minimally invasive repair with early weight bearing rehabilitation has advantages over traditional open repair with delayed mobilisation for patients who have undergone surgery for ruptured Achilles tendon. The practical advantages for patients in early weight-bearing mobilisation were earlier return to normal walking and stair climbing than their open group counterparts. We would, therefore, advocate the use of minimally invasive procedure with early weight-bearing mobilisation for the rehabilitation of all patients with acute ruptures of the Achilles tendon

Background: The Achilles tendon is the strongest and thickest tendon in the human body, it is very commonly injured with significant clinical implications. The treatment of Achilles tendon rupture is a matter of controversy in orthopedics and sports medicine. Surgical repair compared with conservative treatment is debated constantly in the literature, without a conclusive decision. The diagnosis of Achilles tendon rupture is based usually on clinical examination, and may be reinforced by ultrasound or magnetic resonance imaging. The present study has been conducted in order to determine whether an ultrasound examination performed at the time of injury could be useful in deciding how to treat the patient. Patients: Over a period of 5 years we treated 26 patients who had a clinical presentation of ruptured Achilles tendon with ultrasound diagnosis of either a partial tear or a full tear. Patients who were diagnosed by ultrasound as having a full tendon tear were operated on, and were not included in this study. Eight patients had partial tear of the tendon, six had a tear of the musculotendinous region, and twelve had a proximal tear. All patients were treated by means of a cast or a dressing, with limitation of weight bearing. The follow-up period ranged between six months to three years after the injury, and included up-to-date functional evaluation. Results: Eighteen patients were available for evaluation. Excellent functional results were reported by five patients, twelve patients reported good results, and one patient complained of a bad result. None of our patients needed delayed surgery, and only one suffered from re-rupture of the tendon during his rehabilitation, and was treated conservatively with good results. No correlation was found between the location of the tear and the functional results. Conclusions: 1. Ultrasound is an important and accurate tool in the diagnosis of Achilles tendon tear and is helpful in choosing the appropriate treatment. 2. Partial tear of Achilles tendon is not an indication for operative treatment, even when the clinical examination (Thompson test) is positive. The outcome of conservative treatment in this situation is as good or even better than surgical treatment

Control and ruptured Achilles tendons underwent lectin staining with Aleuria aurantia, Canavalia ensiformis, Galanthus nivalis, Phaseolus vulgaris, Arachis hypogea, Sambucus nigra, Triticum vulgaris. The mean pathology score of ruptured tendons was significantly greater than that of control Achilles tendons from individuals with no known tendon pathology. Four of the seven lectins used exhibited significantly positive results. Ruptured tendons show different lectin staining properties than non-ruptured ones. This difference may results from post-translational changes in the extracellular matrix producing biochemical alterations which might interfere with the interaction with the lateral sugar residues of the collagen molecules, or cause steric blockade. To ascertain whether lectins could be a useful tool for investigation of the extracellular matrix of degenerated and normal tendons, haematoxylin-eosin stained slides were assessed blindly using a semi-quantitative grading scale for fibre structure; fibre arrangement, rounding of the nuclei; regional variations in cellularity; increased vascularity; decreased collagen stainability; hyalinisation; glycosaminoglycan, with a pathology score giving up to three marks per each of the above variables, with 0 being normal, and 3 being maximally abnormal. For lectin staining with Aleuria aurantia, Canavalia ensiformis, Galanthus nivalis, Phaseolus vulgaris, Arachis hypogea, Sambucus nigra, Triticum vulgaris, assessment of staining on a scale from 0 (no staining) to 5 (strong staining) was performed blindly. The mean pathology sumscore of ruptured tendons (n=14; average age 46.5 years, range 29–61) was significantly greater than the mean pathology score of the control tendons of Achilles tendons from individuals with no known tendon pathology (n=16; average age 62.5 years, range 49–73) (pathology score: 18.5 ± 3.2 vs 6.1 ± 2.3) Four of the seven lectins used exhibited significantly positive results. Ruptured tendons are histologically significantly more degenerated than control tendons. Ruptured tendons show different lectin staining properties than non-ruptured ones. This difference may result from post-translational changes in the extracellular matrix producing alterations in the biochemistry of the tendon which might interfere with the interaction with the lateral sugar residues of the collagen molecules, or cause steric blockade

Introduction. The exact mechanisms leading to tendinopathies and tendon ruptures remain poorly understood while their occurrence is clearly associated with exercise. Overloading is thought to be a major factor contributing to the development of tendon pathologies. However, as animal studies have shown, heavy loading alone won't cause tendinopathies. It has been speculated, that malfunctioning adaptation or healing processes might be involved, triggering tendon tissue degeneration. By analysing the expression of the entirety of degrading enzymes (degradome) in pathological and non-pathological, strained and non-strained tendon tissue, the aim of this study was to identify common or opposite patterns in gene regulation. This approach may generate new targets for future studies. Materials and Methods. RNA was extracted from different tendon tissues: normal (n=7), tendinopathic (n=4) and ruptured (n=4) Achilles tendon; normal (n=4) and tendinopathic (n=4) posterior tibialis tendon; normal hamstrings tendon with or without subjection to static strain (n=4). The RNA was reverse transcribed, then pooled per group The expression of 538 protease genes was analysed using Taqman low-density array quantitative RT-PCR. To be considered relevant, changes had to be at least 4fold and measurable at a level below 36 Cts. Results. In general, there was little common regulation when exercised was compared with pathological tissue. The expression of PAMR1 and TNFαIP3 was upregulated with exercise (169-fold and 78-fold), Achilles tendinopathy (9724-fold and 7-fold) and Achilles tendon rupture (1809-fold and 10-fold), while DDI1, PSMB11 and PSH2 which were down-regulated with exercise were upregulated with Achilles pathology. Discussion. The newly found targets may deliver insights into the initiation and progression of tendon pathologies: PAMR1, a regeneration associated muscle protease which has been shown to be downregulated in Duchenne muscular dystrophy and upregulated in regenerating muscle fibers, might also be involved in tendon regeneration; TNFαIP3, which negatively regulates the NF-κB/pro-inflammatory pathway, could have anti-inflammatory function in tendon regeneration. PSMB11 and PSH2 are for the first time shown to be expressed in tendon and regulated in tendon pathology. Using this approach we were able to generate new targets and to add information on function, regulation and expression sites of recently identified proteins

Aims. The aim of this meta-analysis was to assess the prognosis after early functional rehabilitation or traditional immobilization in patients who underwent operative or nonoperative treatment for rupture of the Achilles tendon. Methods. PubMed, Embase, Web of Science, and Cochrane Library were searched for randomized controlled trials (RCTs) from their inception to 3 June 2020, using keywords related to rupture of the Achilles tendon and rehabilitation. Data extraction was undertaken by independent reviewers and subgroup analyses were performed based on the form of treatment. Risk ratios (RRs) and weighted mean differences (WMDs) (with 95% confidence intervals (CIs)) were used as summary association measures. Results. We included 19 trials with a total of 1,758 patients. There was no difference between the re-rupture rate (RR 0.84 (95% CI 0.56 to 1.28); p = 0.423), time to return to work (WMD -1.29 (95% CI -2.63 to 0.05); p = 0.060), and sporting activity (WMD -1.50 (95% CI -4.36 to 1.37); p = 0.306) between the early functional rehabilitation and the traditional immobilization treatment strategies. Early rehabilitation up to 12 weeks yielded significantly better Achilles tendon Total Rupture Scores ((ATRS) WMD 5.11 (95% CI 2.10 to 8.12); p < 0.001). Patients who underwent functional rehabilitation had significantly lower limb symmetry index of heel-rise work ((HRW) WMD -4.19 (95% CI -8.20 to 0.17); p = 0.041) at one year. Conclusion. Early functional rehabilitation is safe and provides better early function and the same functional outcome in the longer term. Cite this article: Bone Joint J 2021;103-B(6):1021–1030