Objective: Decreased quadriceps strength may contribute to anterior knee pain after total knee arthroplasty (TKA). The quadriceps force necessary to establish full extension is strongly dependent on the position and the relative length of the lever arms over the knee joint. The purpose of this in vitro study was to investigate the amount of quadriceps force required to extend the knee isokinetically after TKA in dependence of different prosthesis designs and the state of the posterior cruciate ligament (PCL).

Methods: Eight fresh frozen human knee specimens were tested in a kinematic device that simulated an isokinetic knee extension cycle from 120° of flexion to full extension. Knee motion was driven by a hydraulic cylinder applying sufficient force to the quadriceps tendon to produce an extension moment of 31 Nm. The quadriceps force was measured using a load cell attached to the quadriceps tendon after implantation of a cruciate retaining (CR) TKA (Genesis II, Smith& Nephew, Memphis, Tn, USA) applying a conventional and a highly conforming polyethylene (PE) inlay before and after resection of the PCL. Finally, the femoral component of the CR TKA was replaced by a posterior stabilized (PS) design and measurements were redone.

Results: No significant differences in the average quadriceps force were detected between the different PCL retaining inlays (CR, highly conforming) as long as the PCL was intact. However, after resection of the PCL, the required quadriceps force increased significantly for both designs (CR: 4.7%, p < 0.01, Highly conforming: 3.5%, p < 0.03). After implantation of the PS femoral component quad force decreased to its initial levels with forces significantly lower compared to the PCL deficient knees provided with a CR (−6.0%, p < 0.01) or highly conforming (−5.1%, p < 0.01) inlay. With a PS design average quadriceps extension force was not significantly different from cruciate retaining TKA inlays at an intact PCL.

Conclusions: The data of this in vitro study suggest that the quadriceps extension force is significantly higher for knees after cruciate retaining TKA with PCL deficiency, independent of the use of a CR or DD inlay. Thus, the integrity of the PCL should be secured in clinical practice when using a cruciate preserving TKA design.

INTRODUCTION: The techniques to stabilize the patella can be divided into two groups: the first group seeks to change the direction of the extensor mechanism in order to medialize the extending force vector of the quadriceps muscle, e.g. by a distal medialization of the tibial tuberosity or a proximal realignment; the second seeks to reconstruct the medial patellofemoral ligament (MPFL). The goal of this study was therefore to measure changes in patellofemoral kinematics in the intact, MPFL deficient knee, after medial transfer of the tibial tuberosity, after proximal realignment as well as after reconstruction of the MPFL.

METHODS: Eight fresh frozen right knee specimens were mounted in a knee simulator in which isokinetic flexion-extension motions were simulated. Extension cycles were simulated from 120° flexion to full knee extension with an extension moment of 31 Nm. Movement of the patella relative to the femur was measured using an ultrasound based 3D motion analysis system (Zebris, Isny, Germany). During the first test cycles, patellar movement under intact knee conditions were measured, while a constant 100 N laterally oriented force was applied by means of a steel cable attached to the patella. Subsequently, patellar movement was again measured after: transecting the MPL (deficient knee), performing a medialization of the tibial tuberosity, after reconstruction of the transected MPL using a semitendinosus autograft and after proximal realignment.

RESULTS: The patella of the intact knee moved along a medial path with a maximum attained position of 8.8 mm at 25° of knee flexion. The patella of the deficient knee moved up to 4.6 mm (p=0.04) in the medial direction at maximal extension at 30° of knee flexion. After medial transfer of the tibial tuberosity patellar movement reached a maximum medial position of 12.8 mm (p=0.04) at 22° of knee flexion with the laterally oriented force. With a reconstructed MPL, the patella attained a maximum medial position 14.8 mm (p=0.04) at 24.0° of knee flexion. Following proximal realignment, the patella moved on a medial, but significant (p=0.03) different path up to 13.8 mm medially at 30° of knee flexion. In addition, following medialization of the tibial tuberosity and proximal realignment, the center of the patella was significantly (p=0.03) more internally rotated (tilted) than the physiologic patella.

DISCUSSION: The shape of the movement curves after the stabilizating procedures resulted in a medialization relative to intact and deficient conditions. With the reconstructed medial patellofemoral ligament, the patella moved along the most medially oriented path with physiologic tilting. The results suggest that a semi-tendinous autograft can provide sufficient stabilization to prevent lateral displacement or subluxation with physiologic patellar tilt.

Introduction: Non-invasive prediction of load bearing capacity during consolidation of distraction osteogenesis and fracture healing would represent a significant advance in the treatment of patients by defining the appropriate point of time for the removal of the fixator externe. Thereby the risk of refracture, malunion and infection could be reduced. Several methods have been proposed in the past to predict the load bearing capacity: dual-energy x-ray absorptiometry (DEXA), stiffness measurements, quantitative computed tomography, quantitative radiography and ultrasound. In this ex-vivo study stiffness- and DEXA-measurements were compared regarding their suitability to predict the load bearing capacity of bone regenerate.

In addition this study analysed how compressive, bending and torsional stiffness as suitable tools were related to the torsional load bearing capacity using a common set of bone regenerate samples of 26 sheep treated with distraction osteogenesis.

Material and Methods: After osteotomy the sheep tibiae were stabilized using an external half-ring Ilizarov fixator. Followed by a 4-day latency period the tibiae were distracted at a rate of 1.25 mm per day in two increments for 20 days. On the 74th day the sheep were sacrificed and tibiae were harvested. The ends of the specimens were embedded in PMMA for further biomechanical testing. Therefore, the specimens were mounted to a sequence of special costume made jigs for compressive testing, 4-point bending and torsional testing in a material testing machine. Stiffness was calculated by regression of the linear part of the load-displacement curves. The maximum torsional moment of the specimens was determined in a final experiment. In addition the bone mineral density (BMD) of the distracted bone tissue was measured using DEXA. The correlation between the maximum torsional moment and the various types of stiffness respectively BMD was analysed to gain information about the suitability predicting the load bearing capacity.

Results: Torsional stiffness exhibits the highest correlation with the maximum torsional moment (r2 = 0.77) followed by bending (ap (r2 = 0.70); ml (r2 = 0.66)) and compressive stiffness (r2 = 0.60). The correlation for BMD with the maximum torsional moment was smallest (r2 = 0.39).

Discussion: This ex-vivo study revealed that the stiffness measurements seem to be a helpful tool to predict the load bearing capacity of bone regenerate. The results of this study showed stiffness measurements as a more suitable mean to determine the load bearing capacity. Within the various types of stiffness measurements torsional stiffness measurements perform slightly better than bending and compressive stiffness measurements. Nevertheless, further studies are necessary to support the results of this study since the specimens failed applying torsional stress.

INTRODUCTION The ability to evaluate the alignment of total knee arthroplasty using postoperative radiographs might be confounded by limb rotation. The aim of the presented study was therefore to measure the effect of limb rotation on postoperative radiographic assessment and to introduce a mathematical correction to calculate the true axial alignment in cases of a confounded radiograph.

METHODS A synthetic lower left extremity (Sawbones®, Inc,Vashon Island, WA) was used to create a total knee arthroplasty of the Interax I.S.A.® knee prosthesis system (Stryker, Limerick, Ireland). Laser guided measurement of the tibia showed a femoral valgus angle of 6.5° postoperatively. The model was fixed in an upright stand which positioned the limb in varying degrees of rotation. Four series of 10 antero-posterior (AP) radiographs were taken with the knee in full extension, with femoral limb rotation ranging from 20° external rotation to 20° internal rotation in respect to the x-ray beam, in 5° increments. After digitizing each radiograph, four observer independently measured the femoral valgus angle for each series of the long leg radiographs using a digital measurement software (MEDICAD®, Hectec, Altfraunhofen, Germany). Each observer was instructed to determine the femoral valgus angle following the software’s guidelines. In addition each observer measured the geometrical distances of the femoral component figured on the radiographic film. Using a student t-test, the effect of femoral limb rotation on the measured femoral valgus angle and a correlation between femoral rotation and femoral valgus angle was established. Then for each limb rotation the distances ratio was determined to calculate the limb rotation.

RESULTS Without an application of femoral rotation the femoral valgus angle was measured radiographically to be 6.5° (SD 0.4°). With external femoral rotation the measured femoral valgus angle linearly decreased to a minimum of 4.5° (SD 0.2°) at 20° femoral rotation. The linear regression (R2=0.94) calculated a 0.09° change of radiographically measured femoral valgus angle per femoral rotation angle. With the femoral rotation the radiographically measured ratio decreased linearly (R2=0.98) with further internal rotation.

DISCUSSION The results of the presented study suggest a significant influence of femoral rotation during radiographic evaluation of limb alignment after total knee arthroplasty. With further external femoral rotation the radiographically apparent femoral valgus angle decreases. As the apparent femoral valgus angle changes linearly, a calculation of the distances of the particular femoral component could be used to determine the real femoral valgus angle in cases of femoral limb rotation.

Introduction: The goal of meniscal transplantation is to prevent progressive joint degeneration that predictably follows meniscectomy. The meniscal transplant’s ability to transfer load effectively depends on its ability to bear circumferential loading. Purpose of this study was thus to investigate the influence of intraoperative pre-tensioning on the chondroprotective of meniscal transplants in a sheep model.

Methods: Thirty-six sheep were divided into 6 groups (n = 6), subjected to a sham operation (group A), a meniscectomy (group B), or a meniscal autograft using tag sutures with different levels of pre-tensioning (group C, 0 N; group D, 20 N; group E, 40 N; group F, 60 N). Macroscopic (International Cartilage Repair Society score) and histological evaluation (Mankin score) of the articular cartilage was performed after 6 months.

Results: Higher suture pretension (40 N, 60 N) resulted in less cartilage degeneration than in meniscectomized (P =.047; P =.036) and non-pre-tensioned (P =.028; P =.015) knees, with International Cartilage Repair Society scores of 1.63 +/− 0.57 and 1.66 +/− 0.51 in groups E and F, respectively, and scores of 2.40 +/− 0.27 and 2.68 +/− 0.46 observed after meniscectomy and meniscal transplantation with no pre-tensioning, respectively. Group F had a significantly better Mankin score of 6.66 +/− 2.15 (P =.05) compared with group D. Regarding criterion cells, trends toward less degeneration compared with meniscectomized and non-pretensioned knees (P =.054 and P =.055) were found. The coefficient of variation of the Mankin scores was greater than that of the International Cartilage Repair Society score. Group A had significantly better cartilage than all other groups.

Conclusion: Adequate intraoperative pre-tensioning has an influence on the chondroprotective effect of meniscal transplants but did not prevent the development of articular cartilage degeneration.

Clinical Relevance: The results suggest that intraoperative pre-tensioning could improve the chondroprotective effect of meniscal transplantation.

Introduction: Homogenous cell distribution and suffi-cient initial scaffold stability remain key issues for successful tissue engineered osteochondral constructs. The purpose of this study was to investigate the application of initial compression forces during the first 24 hours of cell culture followed by different stress patterns.

Methods: Bone marrow stromal cells were harvested from the iliac crest during routine trauma surgery. The cells were expanded in a 2-dimensional culture and then seeded into the biologic hybrid scaffold with a concentration of 1x10E6 cells per ml. Pressure and vacuum forces were applied in a specially developed glass kit. The constructs were exposed to two different protocols of compression combined as oteochondral matrices of CaReS (collagen I) and Tutobone (Ars Arthro, Esslingen, Germany and Tutogen Medical GmbH, Neunkirchen a. Br., Germany). Controls were resected osteochondral fragments from patients with articular fractures and uncompressed constructs. These effects were evaluated using light microscopy after standard staining to identify matrix penetration. Biomechanical tests were conducted, too using a modified biomechanical testing machine. The ‘constrained compression’, maximum load to failure, modulus, and strain energy density were determined.

Results: Histology: Penetration and cell distribution was demonstrated homogenous and vital, respectively. Mechanical tests showed a significant enhancement of primary matrix stability. The following stress patterns did not enhance significantly stability over seven days.

Discussion: The aim of this project was to investigate the response and cell distrubution of human bone marrow stromal cells seeded on a 3-dimensional biologic hybrid scaffold using compression and vacuum forces.

The integration of mechanical stimulation in the tissue engineering process may lead to a progress in the structural and biomechanical properties of these tissues and offers new possibilities in the management of bone injuries and degenerative diseases.

Introduction: The amount of loading on the cruciate ligaments depends on the tension of the external muscular structures. In vivo studies using EMG have observed a proprioreceptive eccentric co-contraction of the hamstrings during isokinetic knee extension motion. This antagonistic co-contraction increases the quadriceps force necessary to produce the same extension moment on the knee, whereas the loading on the anterior cruciate ligament was measured to be reduced, with the loading on the posterior cruciate ligament to be increased. The objective of this study was thus to investigate the effect of simulated proprioreceptive co-contraction of the hamstrings muscles on quadriceps force, as well as on the relative loading on the cruciate ligament structures during knee extension under dynamic conditions and physiologic loads.

Methods: Five fresh frozen knee specimen were tested in isokinetic extension. Bow shaped loading transducers were fixed in the medial fibres of the anterior (ACL) and posterior cruciate ligament (PCL). The test cycle simulated an isokinetic extension cycle from 120 degrees of flexion to full extension, a hydraulic cylinder thereby applied sufficient force to the quadriceps tendon in a closed-loop control cycle to produce a constant extension moment of 31 Nm about the knee. A second hydraulic cylinder simulated a 200 N co-contraction force of the hamstrings tendons. The loading on the ACL and PCL was first measured in the absence of hamstrings force, and subsequently under constant co-contractive flexion force.

Results: In the absence of hamstring tension, the maximum quadriceps force was 1190 N ( SD 204 N) at 105 degrees of knee flexion. The loading on the ACL was reduced at larger flexion angles, the loading pattern of the PCL showed an inverse relationship with less loading at full extension. The maximum loading in the ACL was 161 N (SD138 N) and maximum tension in the PCL was 38.2 N (SD 34.9). With hamstring co-contraction, maximum quadriceps force increased 19.9 % ( SD 21.0% p= 0.33), maximum tension in the ACL decreased 71.9% (SD 74.3%, p=0.03), and maximum tension in the PCL increased 73.0% (SD 40.9%, p=0.03).

Discussion: This experimental setup enabled direct in vitro measurement of ACL and PCL loading during simulated isokinetic extension motions. The loading on the ACL was dependent on the knee flexion angle. We observed that co-contraction of the hamstrings reduces loading on the anterior cruciate ligament without a significant concomitant increasing the quadriceps muscle force. Our results support the hypothesis that antagonistic co-contraction of the hamstrings during extension of the knee provides an important protective function. In contrast, loading in the posterior cruciate ligament increased during hamstring activation at higher knee flexion angles.

Introduction Non-invasive prediction of load bearing capacity is an important issue in the advanced clinical treatment of distraction osteogenesis in order to define the appropriate point of time for the removal of the external fixateur. Therefore, non-invasive stiffness measurements were recommended as a promising tool due to the high correlation between strength and various kinds of stiffness: Torsional, bending and compressive.

However, previous experiments only analysed the relationship between a single type of stiffness. This approach neglects the multi-dimensional characteristics of bone loading in compression, bending and torsion.

This study investigates how compressive, bending (ap and ml) and torsional stiffness are related to the torsional load bearing capacity of healing callus tissue using a common set of bone regenerate samples of sheep treated with distraction osteogenesis. In addition, this study compares the evolution of the various kinds of stiffness.

This study provides insight into how the various stiffness modes are suited to predict the load bearing capacity by in-vivo stiffness measurement.

Material and Methods Mid-diaphyseal osteotomies were performed in 26 right tibiae of mature, female domestic sheep. Tibiae were then stabilized using an external half-ring Ilizarov fixator. After a 4-day latency period the tibiae were distracted at a rate of 1.25 mm per day in two increments for 20 days. As a result of a parallel study, the callus was treated with different combinations of growth factors and carrier material resulting in four treatment groups plus a contralateral control group. The sheep were sacrificed and the tibiae were harvested on the 74th day.

The ends of the tibiae were embedded in PMMA and mounted to a sequence of special custom made jigs for compressive testing, 4-point-bending and torsion in a material testing machine.

Stiffness was calculated by regression of the initial linear part of the load-displacement curves.

In a final experiment, the specimens were loaded in torsion until failure to record the ultimate torsional moment.

Results Torsional stiffness exhibits the highest correlation with the ultimate torsional moment (r2 = 0.77), while the ones for compressive (r2 = 0.60) and bending (ap (r2 = 0.70); ml (r2 = 0.66)) are only slightly lower.

Discussion This ex-vivo study in sheep shows that torsional, bending (ap and ml) and compressive stiffness measurements are all suitable means to predict the load bearing capacity of healing callus tissue. Our results show that torsional stiffness measurements perform slightly better than compressive and bending stiffness measurements. However, further studies are necessary to underline the superior performance of torsional stiffness measurements, since the sheep-tibiae were failed by applying torsional stress.

Introduction: This biomechanical study evaluates the consequences of a mid-third BPTB-autograft excision on patellofemoral biomechanics and knee kinematics. Of particular interest was the potential role of a BPTB-autograft excision on postoperative anterior knee pain in ACL replacement surgery.

Methods: Isokinetic knee extension from 120 of flexion to full extension was simulated on 9 human knee cadaver specimens (5 male, 4 female, average age at death 43 years). Joint kinematics was evaluated by ultrasound sensors (CMS 100TM, Zebris, Isny, Germany), and retro-patellar contact pressure was measured using a thin-film resistive ink pressure system (K-ScanTM 4000, Tekscan, Boston). All data were taken before and after excision of a mid-third BPTB-autograft.

Results: Following excision of a mid-third patella tendon autograft we found a significant (p< 0.05) proximalization of the patella (average: 0.5 mm) and a significant decrease of patella flexion in the sagittal plane (average: 1). Patella tilt, -rotation (frontal plane), -translation (medial/lateral) and tibiarotation (external-/internal), -axis (varus-/valgus position) remained unchanged. Patellofemoral contact pressure and -area decreased significantly near knee extension (p< 0.05).

Conclusions: We conclude that an excision of a mid-third patella tendon autograft results in a lengthening of the tendon with a proximalization of the patella. As the patellofemoral pressure decreases and the patella remains centralized, postoperative anterior knee pain following ACL-replacement using a BPTB autograft can not be explained by the results of our study.

Objective: To determine the effect of intraoperative pre-tensioning of meniscal transplants on the tibial plateau in an animal experiment:

Material and Methods: Thirty-six sheep were used for this animal study. The animals were divided into 6 groups: -group A was the sham group; – in group B medial meniscectomy was performed; in group C-F medial meniscus transplantation with an autograft was carried out. In group C-F different defined pre-tensioning was applied to meniscal transplants via bone tunnel sutures (0N, 20N, 40N and 60N respectively). After 6 months the animals were sacrificed. The lower limb specimen were placed in a material testing machine under standard conditions in 30, 60 and 90 degrees of flexion and loaded through the femoral axis to 500N. For determining contact area a thin film pressure transducer (Tekscan) was positioned underneath the medial meniscus. Statistical analysis was performed using Mann-Whitney test.

Results: The mean contact pressure of the sham group and the groups with the transplanted meniscus was significantly lower in relation to meniscectomized knees. Significant increases in contact area and reductions in in peak contact pressure could be identified. At greater flexion angles only the meniscal transplantation group with the 40N pretension showed a significant increase of contact area and/or very strong trend in relation to meniscectomized knees. Concerning peak contact pressure, all meniscal transplantated groups with exception th 0N pre-tension group showed significant reduction in comparison to the meniscectomized group.

Conclusion: Regarding the results we can conclude that the biological ingrowth has an influence on the biomechanical effect of meniscal transplantation. For this animal model and with the compressive load of 500N especially 40N pre-tension of meniscal transplants seems to be efficient to provide load transmission function of the meniscus.

Introduction: There is a need for new non-invasive, predictable and quantifiable techniques to assess the process of fracture healing and remodelling in bone. There are several methods to monitor the bone healing in-vivo. But these methods either fail as quantitative predictors of the healing process (X-ray) or exhibit complicated and expensive measurement principles. Some known in-vivo stiffness measurement methods have several disadvantages including the risk of bone malalignment. Therefore we compared ex-vivo torsional strength of bone with in-vivo torsional stiffness under minimal load in two animal model of distraction osteogenesis. Additionally the device was tested in an ex-vivo model.

Methods: An external fixator was combined with a rotating double half-ring. The measurement device was fixed to the half-ring during measurements. It was equipped with a linear variable differential transducer, a load cell, and a stepper motor. During measurements the two parts of the half-ring were rotated against each other and the load and displacement were recorded. The slope coefficient after performing a linear regression between data points of moment and displacement curve was defined as stiffness. Afterwards all models were tested in a material testing system as gold standard. This was tested in an in-vivo animal study of tibial distraction (minipigs time of consolidation 10 days/sheeps time of consolidation 50 days).

Results: Between in-vivo initial torsional stiffness and torsional strength in minipigs we found a highly significant (p=0.001) coefficient of determination of 0.82, but we found only a poor correlation (p> 0.05) in sheeps. However, the results of the ex-vivo model showed a high precision and accuracy.

Discussion: The results of this study suggest that the bone regenerate strength of healing bones can be assessed in-vivo by the presented inital stiffness measurement method in the beginning of an early stage of healing as shown in minipigs. But at the end of the healing period the correlation of strength and stiffness leveled off. There is a similar model showing an excellent correlation, that agree with our data. They explained the weakening of the correlation at the end of healing by a transformation of early bone to lamellar bone after a 2/3 consolidation. In summary, the presented device could be a reliable future tool to monitor the healing progress in patients with bone malalignement or fractures in the beginning of the healing period.

Purpose: The study was designed to evaluate the biomechanical and neurohistological properties of the infrapatellar fat especially concerning its potential role in the anterior knee pain syndrome.

Methods: Isokinetic knee extension from 120 of flexion to full extension was simulated on 10 human knee cadaver specimens (6 male, 4 female, average age at death 44 years). Joint kinematics was evaluated by ultrasound sensors (CMS 100TM, Zebris, Isny, Germany), and retro-patellar contact pressure was measured using a thin-film resistive ink pressure system (K-ScanTM 4000, Tekscan, Boston). The infrapatellar tissue pressure was analyzed using a closed sensor cell. The patellar contact pressure was measured before and after resection of the infrapatellar fat pad. The distribution of nerve fibres in the infrapatellar fat pad was assed immunohistologically in a second part of the study.

Results: Infrapatellar tissue pressure significantly increased during knee extension < 20 and flexion > 100 ranging from 343 (223) mbar at O- to 60 (64) mbar at 60 of flexion. Total resection of the infrapatellar fat pad resulted in a significant decrease in tibial external rotation of 3° in full knee extension (p=0.011), combined with a significant medial translation of the patella between 29 and 69° knee flexion (p=0.017 to 0.028). Retropatellar contact pressure was significantly (p< 0.05) reduced at all flexion angles, at 120° knee flexion more than in full knee extension. Studying all the detectable nerves present in 50 fields (x200 objective) we found an average of 6.4 substance-P- (25%) of a total of 24.7 nerve fibres in the infrapatellar fat pad. There was a significantly (p< 0.01) higher number of substance-P-fibers (24.4 (28%) of 105.7) in the superficial synovial tissue. The number of S-100-fibers was significantly (p< 0.05) higher in the central and lateral part of the fat pad.

Conclusions: Based on these results, we conclude that resection of the infrapatellar fat pad could potentially reduce clinical symptoms in the anterior knee pain syndrome, and that, contrary to commonly believed, the infrapatellar fat pad may have a biomechanical function and play a role in the anterior knee pain syndrome.

Aims: This biomechanical study was performed to evaluate the consequences of a total infrapatellar fat pad resection on knee kinematics and patellar contact pressure. Methods: Knee motion between 120∞ of flexion and full extension was performed in a knee kinemator on 10 fresh frozen knee specimens (6 male, 4 female, average age 44 years). The joint kinematics was evaluated by ultrasound sensors (Zebris-system), the patellar contact pressure was measured using a thin-film resistive ink pressure system (Tekscan). All data were taken before and after resection of the infrapatellar fat pad and statistically analyzed. Results: A total resection of the infrapatellar fat pad resulted in a significant (p< 0,05) decrease of the tibial external rotation in knee extension combined with a significant (p< 0,05) medial translation of the patella. The patellar contact pressure was significantly (p< 0,05) reduced, in knee flexion more than in knee extension. Conclusions: We conclude that a resection of the infrapatellar fat pad might reduce clinical symptoms in the anterior knee pain syndrome. A biomechanical function of the infrapatellar fat is suspected.