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Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_16 | Pages 19 - 19
1 Apr 2013
Harada N Watanabe Y Abe S Sato K Yamanaka K Sakai Y Kaneko T Matsushita T
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Purpose

The purpose of this study was to evaluate the effects of implantation of mesenchymal stem cell derived condrogenic cells (MSC-DC) on bone healing in segmental defects in rat femur.

Methods

Five-millimeter segmental bone defects were produced in the mid-shaft of the femur of Fisher 344 rats and stabilized with external fixator. The Treatment Group received MSC-DC, seeded on a PLGA scaffold, locally at the site of the bone defect, and Control Group received scaffold only. The healing processes were monitored radiographically (Softex), and studied radiographically (Micro-CT) and histologically.


Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_16 | Pages 83 - 83
1 Apr 2013
Sato K Watanabe Y Abe S Harada N Yamanaka K Sakai Y Kaneko T Matsushita T
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Introduction

what size of defect is optimal for creating an atrophic nonunion animal model has not been well defined. Our aim in this study was to establish a clinically relevant model of atrophic nonunion in rat femur by creation of a bone defect to research fracture healing and nonunion.

Materials and methods

We used 30 male Fischer 344 rats (aged 10–11 weeks), which were equally divided into six groups. The segmental bone defects to a single femur in each rat were performed by double transverse osteotomy, and different sized defects were created by group for each group (1 mm, 2 mm, 3 mm, 4 mm, 5 mm and 6 mm). The defects were measured and maintained strictly by using an original external fixator. The periosteum for each defect was stripped both proximally and distally. Thereafter, these models were evaluated by radiology and histology. Radiographs were taken at baseline and at intervals of two weeks over a period of 8 weeks. Atrophic nonunion was defined as a lack of continuity and atrophy of both defect ends radiologically and histologically at eight weeks.


Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_16 | Pages 35 - 35
1 Apr 2013
Sato K Watanabe Y Abe S Harada N Yamanaka K Sakai Y Kaneko T Matsushita T
Full Access

Reconstruction of 10mm segmental bone defects in rat by mesenchymal stem cell derived chondrogenic cells (MSC-DC)

Background

Mesenchymal stem cell derived condrogenic cells (MSC-DC) have excellent potential for healing 5 mm bone defect in rat femur.

Purpose

To evaluate the effectiveness of MSC-DC on bone healing in 10 mm segmental bone defects in rat femur.