Introduction

Total ankle replacement (TAR) has been used as a surgical intervention for arthritis since the 1970s. However, unlike clinically successful hip and knee replacements, TARs are renowned for extensive contraindications to surgery and high failure rates with an average of 83% survival at 5 years. The majority cite aseptic loosening as the reason for failure. The aim of this study wais to analyse retrieved TARs visually and through interferometry to identify potential the failure mechanisms associated with these devices.

Introduction

Surgical treatment options for osteoarthritis of the first MTPJ include fusion, excision arthroplasty, interposition arthroplasty and replacement arthroplasty. ¹ Arthroplasty of the first MTPJ is not a treatment modality that is, as yet, widely accepted. ^2,3 although early results are promising.

The Toefit-plus (tm) first MTP joint arthroplasty is an uncemented modular hemi or total joint replacement. The aim of this study was to look at the short to medium term outcome of the Toefit-plus(tm) system, performed by a single surgeon in a district general hospital.

Introduction: With increasing availability of CT scans their use in the investigation of the subtalar joint increases, whilst we continue to use plain x-ray. Using a standardised reporting protocol, we graded x-rays and CT scans to compare the diagnosis made using each modality.

Materials and Methods: An atlas and reporting system of the subtalar joint was designed using a modification of Kellgren and Lawrence’s system. 50 consecutive CT scans of the subtalar joint were identified and saved along with paired plain x-rays of the foot and ankle. All investigations were anonymised. Scans were excluded if there were no plain films or there was evidence of previous trauma. Orthopaedic surgeons were asked to report on the 50 CT scans and 50 plain radiographs using the reporting protocol, commenting on two components for each investigation; the anterior and middle facets and the posterior facet of the subtalar joint.

Results: In 33% of cases the facets of the subtalar joint could not be appreciated from the plain x-rays. The difference between the modalities in reported grade of degeneration of the anterior and middle facets of the subtalar joint was statistically significant (p= 0.014) but not for the posterior facet (0.726). When looking at the Spearman correlation coefficient, the anterior and middle facets had no correlation (r = − 0.067) although the posterior facet did (r = 0.029).

Discussion: When looking at the posterior facet of the subtalar joint plain x-rays and CT scans give comparable results. When looking at the anterior and middle facets the information gained from the plain x-rays bears no resemblance to that gained from the CT scans.

Conclusion: The plain x-ray is an inaccurate, unreliable method of investigating degenerate pathology of the subtalar joint and should be superseded, and perhaps replaced, by the CT scan.

Introduction: Kashin-Beck disease (KBD) is a special endemic osteoarthropathy whose main pathologic changes occur in growth plate cartilage and articular cartilage of human limbs and joints where it is manifested as cartilage degeneration and necrosis. Past and current research suggests that KBD, and its endemic geographic distribution in China, is due to the combined presence of fungal mycotoxins (on stored food ingested by affected populations) and a regional selenium deficiency in the environment providing local food sources. Thus, we hypothesise that the presence of fungal mycotoxins and the absence of selenium in the diet specifically affects chondrocyte metabolism in the growth plate during limb and joint development and in articular cartilage of adults, which leads to localised tissue necrosis, and the onset of degenerative joint disease. The aim of this study was to examine the effects of mycotoxins; e.g. Nivalenol (NIV), selenium and NIV in the presence of selen! ium in in vitro chondrocyte culture systems to better understand cellular and molecular mechanisms underlying the pathogenesis of KBD.

Methods: Chondrocyte tissue cultures were established using cartilage explant cultures either in the presence or absence of selenium (0.5–1.5 microg/ml) and the mycotoxin nivalenol (0.5–1.5 microg/ml) and culture for 1 to 4 days. Medium was harvested daily at day 1 through 4 and analysed for glycosaminoglycan (GAG) release and the presence of aggrecanase or MMP activity using RT-PCR for gene expression and monoclonal antibodies that detect their respective enzyme-generated neo-epitopes on cartilage aggrecan metabolites.

Results: Our studies to date have shown that NIV exposure induces catabolic changes in chondrocyte metabolism with an increased expression of aggrecanase activity. Addition of selenium did not affect mRNA expression of the aggrecanases ADAMTS-4 & 5. Parallel studies involving immunohistochemical analyses of articular cartilage from KBD showed an increase in aggrecanase activity.

Conclusions: These studies demonstrate that induction of aggrecanase activity as one of the molecular mechanisms involved is the pathogenesis of KBD. However, the addition of selenium does not alter aggrecanase gene expression indicating that its beneficial effects are occurring in other areas of cartilage metabolism.

Introduction: Kashin-Beck disease (KBD) is an endemic osteoarthropathy with pathological changes occurring in growth plate and articular cartilage in humans. It manifests as cartilage degeneration and necrosis. It is postulated that KBD is due to fungal mycotoxins infiltrating the diet and a regional selenium deficiency in the environment providing food sources in a broad belt across China. Previous work has established an in vitro system in which chondrocytes are cultured and an ex vivo cartilage graft is produced. Subjecting these chondrocytes to either selenium (SEL), Nivalenol (NIV) or in combination during the growth of the graft was found to alter the morphology of the cartilage graft. In addition, the quantity of the large aggregating proteoglycan, was significantly reduced in a dose dependent manner in the presence of Nivalenol. This study aimed to examine the composition of aggrecan from grafts grown in the presence of NIV or SEL alone, or in combination to better understand cellular and molecular mechanisms underlying the pathogenesis of KBD.

Methods: Chondrocytes (from 7 day old bovine cartilage) were seeded at high density in MilliCell filter inserts (12mm diameter; Millipore, MA). Cultures were maintained for 4 weeks in DMEM supplemented with 20% heat–inactivated FBS, ascorbate (100μg/ml) and TGFß2 (5ng/ml) or additionally supplemented with either SEL , NIV or both at concentrations of 0.01, 0.05 and 0.1μg/ml. Media was refreshed thrice weekly and later analysed. At 4 weeks the cartilage grafts were harvested, weighed and extracted in 4M guanidium chloride (with an inhibitor cocktail) for biochemical analysis of matrix molecules. Residues were papain digested. Glycosaminoglycan concentration was determined using the DMMB assay in all media samples, guanidine extracts and papain digests. Aggrecan and GAG composition was determined using Western blotting with a panel of antibodies recognising chondroitin sulphate (CS), keratan sulphate (KS) and protein core epitopes present in aggrecan.

Results: The total GAG synthesised in a 4week period was substantially reduced in chondrocytes cultured in the presence of NIV at 0.05 and 0.1μg/ml and to a lesser extent in those cultures exposed to the highest dose of SEL. However, the amount of GAG released into the media remained fairly constant within the treatment groups, but a marked reduction was apparent in the guanidine extracts of the cartilage grafts. Western blot analysis with a series of antibodies on guanidine extracted aggrecan showed no substantial changes in the core protein molecular weights however analysis demonstrated that KS was reduced in NIV treated cultures. Results also indicated that NIV treated cultures appeared to contain less CS substitutions on the aggrecan core protein.

Discussion: The GAG concentration data indicates that there is an inability of the GAG to remain within the cartilage grafts extracellular matrix. when treated with NIV. Western blot analysis indicates minor changes in the composition of the aggrecan in relation to protein core length and CS/KS side chain substitutions or length. Further work will investigate the proportion of aggrecan able to form high molecular weight aggregates, the metabolism of link protein and hyaluronan.

Traditionally midshaft clavicle fractures have been treated conservatively. It is recognized that displaced and shortened fractures may be better treated operatively. In particular, patients with greater than 20 mm of shortening and 100 percent displacement have a symptomatic non union rate of 30 percent. The standard technique used previously has been via plate fixation with LC-DCP or DCP. However in the last 5 years intramedullary fixation has been popularized. “Rockwood intramedulary clavicular pin” remedies the past treatment issues including poor blood supply, painful prominent hardware and stress raiser related to removal of metal work.

Aim: The aim of this study was to assess the functional outcome following intramedullary fixation of clavicle using Rockwood pin.

Method: We retrospectively evaluated 17 patients with displaced and shortened mid-shaft clavicular fractures who underwent intramedullary pinning using Rock-wood pins. Each patient was assessed using the ASES, Constant and SF36 scoring system. A physical examination was performed and individual radiographs were assessed to determine union.

Results: The mean age of the patients was 28 (range 15–56). All patients went into union within 2 to 4 months. They had a shorter stay in hospital, earlier mobilization and no scar related paraesthesia. The average ASES score 98.2 (range 92–100) and constant 95.3 (range 89–100).

Summary: Displaced and shortened mid clavicular fractures require operative fixation. Plates and screws on the clavicle requires significant soft tissue stripping leading to compromised blood supply to the bone and multiple bi-cortical screws act as stress raisers. Previous intra-medullary devices presented with the problem of pin migration. Rockwood pins are designed with a differential pitch which leads to compression at the fracture site and prevent pin migration. From this study we now recommend the use of the Rockwood Pin for the management of displaced mid-shaft clavicle fractures.

Revision hip surgery is becoming increasingly common, 300 procedures being performed in 2001 at our institution. In order to achieve a good outcome bone stock needs to be of good quantity frequently necessitating the use of impaction bone grafting using allograft bone.

Donor bone may frequently take three months before it becomes available for use due to the stringent screening procedure. Donor patients must have a clean bill of health, swabs taken at the time of surgery must obviously demonstrate no growth and blood samples taken at donation and an interval of three months, free from viral infectious diseases. It is thus easy to see the lag from the time of donation to availability and why, with increasing demand, need for allograft bone is rapidly exceeding supply.

We need to look for an alternative supply of human bone allograft.

We have compared the harvest of bone at the time of primary total knee replacement with that of the femoral head by both mass and volume. Sixty consecutive patients undergoing primary hip or knee arthroplasty were included in the study, and the masses and volume of the femoral heads compared with that of the total bone cuts in knee arthroplasty. The type of knee replacement used was documented as was whether the femoral head had had a bone block removed. It was found that the mass of femoral heads was 81g, that of knee cuts 95g this is a statistically significant difference; the volume of femoral heads 66ml and that of knee cuts 75ml.

The volumes of bone available from knee arthroplasty cuts are at least comparable femoral heads obtained using hip replacement and could, perhaps, provide a realistic source of bone allograft.