Entheses are the anchorage sites of tendons to bones in the musculoskeletal system. They have a unique microanatomy that allow smooth transfer of mechanical load through tendon to bone. However, entheses are prone to injury due to their small surface area1,2. The overall success rate of the current gold standard treatment (directly attaching the tendon to bone) is small3,4. Consequently, the aim of this study was to evaluate different hydrogels and their suitability for developing an To create a 3D This study demonstrates that 3D co-cultures can be manufactured