Compressive fracture of osteoporotic vertebrae has been one of the most important health problems in aged societies because severely injured spin might be a reason of bedridden for elderly people. Osteoporosis has been widely assessed by averaged bone mineral density of vertebrae measured using DEXA, however, BMD sometimes does not reflect the strength of vertebrae. CT imaged based finite element method (CT-FEM) has been applied to evaluate the strength of vertebrae based on the biomechanics theory and approved by a part of the highly advanced medical treatment in Japan. In the present study, compressive strength of more than 100 vertebrae were evaluated using CT-FEM, and the correlation between BMD and the strength was thoroughly investigated. It was found that some vertebrae with high BMD could have low strength which may cause fracture easily. Thus, a controversial point of the BMD based diagnosis of osteoporosis was clearly indicated. In this invited talk, some basic theories of CT-FEM and fracture assessment and some key results from the recent study will be presented.

Abstract

Transarticular screws at the C1 to C2 level of the cervical spine provide rigid fixation, but there is a danger of injury to a vertebral artery. The risk is related to the technical skill of the surgeon and to variations in local anatomy.

We studied the grooves for the vertebral artery in 50 dry specimens of the second cervical vertebra (C2). They were often asymmetrical, and in 11 specimens one of the grooves was deep enough to reduce the internal height of the lateral mass at the point of fixation to ≤2.1 mm, and the width of the pedicle on the inferior surface of C2 to ≤2 mm. In such specimens, the placement of a transarticular screw would put the vertebral artery at extreme risk, and there is not enough bone to allow adequate fixation.

Before any decision is made concerning the type of fixation to be used at C2 we recommend that a thin CT section be made at the appropriate angle to show both the depth and any asymmetry of the grooves for the vertebral artery.

Variations in pelvic anatomy are a major risk factor for misplaced percutaneous sacroiliac screws used to treat unstable posterior pelvic ring injuries. A better understanding of pelvic morphology improves preoperative planning and therefore minimises the risk of malpositioned screws, neurological or vascular injuries, failed fixation or malreduction. Hence a classification system which identifies the clinically important anatomical variations of the sacrum would improve communication among pelvic surgeons and inform treatment strategy. 300 Pelvic CT scans from skeletally mature trauma patients that did not have pre-existing posterior pelvic pathology were identified. Axial and coronal transosseous corridor widths at both S1 and S2 were recorded. Additionally, the S1 lateral mass angle were also calculated. Pelvises were classified based upon the sacroiliac joint (SIJ) height using the midpoint of the anterior cortex of L5 as a reference point. Four distinct types could be identified:. Type-A – SIJ height is above the midpoint of the anterior cortex of the L5 vertebra. Type-B – SIJ height is between the midpoint and the lowest point of the anterior cortex of the L5 vertebra. Type-C – SIJ height is below the lowest point of the anterior cortex of the L5 vertebra. Type-D – a subgroup for those with a lumbosacral transitional vertebra, in particular a sacralised L5. Differences in transosseous corridor widths and lateral mass angles between classification types were assessed using two-way ANOVAs. Type-B was the most common pelvic type followed by Type-A, Type-C, and Type-D. Significant differences in the axial and coronal corridors was observed for all pelvic types at each level. Lateral mass angles increased from Types-A to C, but were smaller in Type-D. This classification system offers a guide to surgeons navigating variable pelvic anatomy and understanding how it is associated with the differences in transosseous sacral corridors. It can assist surgeons’ preoperative planning of screw position, choice of fixation or the need for technological assistance

Intra-Discal Vacuum Phenomenon (IDVP) represents an intradiscal nitrogen gas accumulation where a cavity opens in a supine position, lowering intra-discal pressure and generating a bubble. IDVP has been observed in up to 20% of elderly patients and reported in almost 50% of chronic LBP patients. With a highly accurate detection on CT, its significance lacks clarity and consideration within normative data. IDVP occurs with patterns of lumbar and/or lumbopelvic morphology and associated diagnoses. Over-60s population based sample of 2020 unrelated CT abdomen scans without acute spinal presentations, with sagittal reconstructions, inclusive of T12 to femoral heads, were analyzed for IDVP and pelvic incidence (PI). Subjects with diagnostic morphological associations of the lumbar spine, including previous fracture, autofusion, transitional vertebra and listhesis, were selected out and analyzed separately. Subjects were then equally grouped into low, medium and high PI. Prevalence of lumbar spine IDVP is 41.3%. 125 cases were excluded. 1603 subjects yielded 663 IDVP. This was increased in severity towards the lumbosacral junction (L1L2 9.4%, L2L3 10.9%, L3L4 13.7%, L4L5 19.9%, L5S1 28.5%) and those with low PI, while distribution was more even with high PI. 292 had positive diagnostic associations, which were more likely to occur at the level of isthmic spondylolisthesis, adjacent to a previous fracture or suprajacent to lumbosacral transitional vertebra (p<0.05). This study has identified normative values for prevalence and severity of IDVP in a normal aging population. Morphological patterns that influence the pattern of IVDP such as pelvic incidence and diagnostic associations provide novel insights to the function of the aging spine

The current study aims to compare the clinico radiological outcomes between Non-Fusion Anterior Scoliosis (NFASC) Correction and Posterior Spinal Fusion (PSF) for Lenke 5 curves at 2 years follow up. Methods:38 consecutive Lenke 5 AIS patients treated by a single surgeon with NFASC (group A) or PSF (group B) were matched by age, Cobb's angle, and skeletal maturity. Intraoperative blood loss, operative time, LOS, coronal Cobbs, and SRS22 scores at 2 years were compared. Flexibility was assessed by modified Schober's test. Continuous variables were compared using student t-tests and categorical variables were compared using chi-square. The cohort included 19 patients each in group A and B . Group A had M:F distribution of 1:18 while group B had 2:17. The mean age in group A and group B were 14.8±2.9 and 15.3±3.1 years respectively. The mean follow-up of patients in groups A and B were 24.5±1.8 months and 27.4±2.1 months respectively. Mean pre-op thoracolumbar/lumbar (TL/L) cobbs for group A and group B were 55°±7° and 57.5°±8° respectively. At two years follow up, the cobbs for group A and B were 18.2°±3.6° and 17.6°±3.5° respectively (p=0.09). The average operating time for groups A and B were 169±14.2 mins and 219±20.5 mins respectively (p<0.05). The average blood loss of groups A and B were 105.3±15.4 and 325.3±120.4 respectively (p<0.05). The average number of instrumented vertebra between groups A and B were 6.2 and 8.5 respectively (p<0.05). The average LOS for NFASC and PSF was 3.3±0.9 days and 4.3±1.1 days respectively (p<0.05). No statistically significant difference in SRS 22 score was noted between the two groups. No complications were recorded. Our study shows no significant difference in PSF and NFASC in terms of Cobbs correction and SRS scores, but the NFASC group had significantly reduced blood loss, operative time, and fewer instrumented levels. NFASC is an effective alternative technique to fusion to correct and stabilize Lenke 5 AIS curves with preservation of spinal motion

Bone metastases radiographically appear as regions with high (i.e. blastic metastases) or low (i.e. lytic metastases) bone mineral density. The clinical assessment of metastatic features is based on computed tomography (CT) but it is still unclear if the actual size of the metastases can be accurately detected from the CT images and if the microstructure in regions surrounding the metastases is altered (Nägele et al., 2004, Calc Tiss Int). This study aims to evaluate (i) the capability of the CT in evaluating the metastases size and (ii) if metastases affect the bone microstructure around them. Ten spine segments consisted of a vertebra with lytic or mixed metastases and an adjacent control (radiologically healthy) were obtained through an ethically approved donation program. The specimens were scanned with a clinical CT (AquilionOne, Toshiba: slice thickness:1mm, in-plane resolution:0.45mm) to assess clinical metastatic features and a micro-CT (VivaCT80, Scanco, isotropic voxel size:0.039mm) to evaluate the detailed microstructure. The volume of the metastases was measured from both CT and micro-CT images (Palanca et al., 2021, Bone) and compared with a linear regression. The microstructural alteration around the metastases was evaluated in the volume of interest (VOI) defined in the micro-CT images as the volume of the vertebral body excluding the metastases. Three 3D microstructural parameters were calculated in the VOI (CTAn, Bruker SkyScan): Bone Volume Fraction (BV/TV), Trabecular Thickness (Tb.Th.), Trabecular Spacing (Tb.Sp.). Medians of each parameter were compared (Kruskal-Wallis, p=0.05). One specimen was excluded as it was not possible to define the size of the metastases in the CT scans. A strong correlation between the volume obtained from the CT and micro-CT images was found (R2=0.91, Slope=0.97, Intercept=2.55, RMSE=5.7%, MaxError=13.12%). The differences in BV/TV, Tb.Th. and Tb.Sp. among vertebrae with lytic and mixed metastases and control vertebrae were not statistically significant (p-value>0.6). Similar median values of BV/TV were found in vertebrae with lytic (13.2±2.4%) and mixed (12.8±9.8%) metastases, and in controls (13.0±10.1%). The median Tb.Th. was 176±18 ∓m, 179±43 ∓m and 167±91 ∓m in vertebrae with lytic and mixed metastases and control vertebrae, respectively. The median Tb. Sp. was 846±26 ∓m, 849±286 ∓m and 880±116 ∓m in vertebrae with lytic and mixed metastases and control vertebrae, respectively. In conclusion, the size of vertebral metastases can be accurately assess using CT images. The 3D microstructural parameters measured were comparable with those reported in the literature for healthy vertebrae (Nägele et al., 2004, Calc Tiss Int, Sone et al., 2004, Bone) and showed how the microstructure of the bone tissue surrounding the lesion is not altered by the metastases

MicroRNA´s are regulatory sequences which influence the posttranscriptional synthesis of about 70% of protein encoding genes. In different studies, MicroRNA-146a (miR-146a) was associated with inflammatory and autoimmunological processes. In vitro it was shown, that miR-146a influences the bone metabolism by regulating differentiation of mesenchymal stem cells. The miR-146a deficient mouse starts to develop lymphoproliferative and myeloproliferative disease by 6–8 months of age. In this study, we investigate the influence of miR-146a deficiency on bone structure and stability dependent on age and gender. Material and Methods. Male and female mice of wild type (WT) and miR-146a deficient (KO) animals at the age of 2–3 and 5–7 month were analyzed Femur, Tibia and lumbar vertebra (LWK4) were dissected and used für structural analyses by microcomputer tomography (µCT). Parameters like bone volume/tissue volume, trabecular bone volume, trabecular thickness, number and separation as well as cortical thickness were determined. Biomechanical stability as load to failure testing was determined using torsional testing for the long bones and axial compression testing for the vertebra body. Statistical analysis was performed using Graph Pad Prism (Mann-Whitney-U-Test, significance: p<0.05). Results. Structural analyses of the bone structure in the long bones (femur, tibia) revealed a significant higher bone volume/tissue volume (BV/TV) and trabecular bone mass in the elder (5–7 month) miR-146a deficient female mice compared to the male group or wild type animals of either age. In the diaphysis of the femur a BV/TV of 21% was determined for the elder miR-146a deficient females compared to 9% BV/TV in the age matching WT group. These changes were due to an increase in trabecular thickness and trabecular number in this area. In contrast to that, the cortical thickness of all bones analyzed was lowered in the miR-146a deficient animals (male and female) compared to wild type. Biomechanical stability of long bones as well as the vertebra body of the older, female KO group was significantly lower compared to wild type bones. Femurs showed a maximal torque of 20Nmm compared to 34Nmm in the wild type group. The vertebra of the KO mice showed a maximal force at failure of 22N compared to 40N in the wild type group. Male groups and younger females revealed values comparable to wild type animals. Conclusion. The deficiency of miR-146a leads to an increase of trabecular bone in the long bones of female 5–7 month old mice, but to lowered biomechanical bone stability. If this is due to alterations in differentiation or proliferation of mesenchymal stem cells remains unclear and will be analyzed further. Additionally, gender relation of our observations points to the influence of female specific regulatory mechanisms like the involvement of estrogen receptor related mechanisms

Pedicle screws fixation to stabilize lumbar spinal fusion has become the gold standard for posterior stabilization. However their positioning remain difficult due to variation in anatomical shape, dimensions and orientation, which can determine the inefficacy of treatment or severe damages to close neurologic structures. Image guided navigation allows to drastically decrease errors in screw placement but it is used only by few surgeons due to its cost and troubles related to its using, like the need of a localizer in the surgical scenario and the need of a registration procedure. An alternative image guided approach, less expensive and less complex, is the using of patient specific templates similar to the ones used for dental implants or knee prosthesis. Like proposed by other authors we decided to design the templates using CT scans. (slice thickness of 2.0 mm). Template developing is done, for each vertebra, using a modified version of ITK-SNAP 1.5 segmentation software, which allow to insert cylinders (full or empty) in the segmented images. At first we segment the spine bone and then the surgeon chose screw axes using the same software. We design each template with two hollow cylinders aligned with the axes, to guide the insertion in the pedicle, adding contact points that fit on the vertebra, to obtain a template right positioning. Finally we realize the templates in ABS using rapid prototyping. After same in-vitro tests, using a synthetic spine (by Sawbones), we studied a solution to guarantee template stability with simple positioning and minimizing intervention invasiveness. Preliminary ex-vivo animal testing on porcine specimens has been conducted to evaluate template performance in presence of soft-tissue in place, simulating dissection and vertebra exposure. For verification, the surgeon examined post-operative CT-scans to evaluate Kirschner wires positioning. During the ex-vivo animal test sessions, template alignment resulted easy thanks to the spinous process contact point. Their insertion required no additional tissue removal respect to the traditional approach. The positioning of contact points on vertebra's lamina and articular processes required just to shift the soft tissue under the cylinders bases. The surgeon in some cases evaluated false stable template positions since not each of the 4 contact points were actually in contact with the bone surface and tried the right position. CT evaluation demonstrate a positive results in 96.5% of the Kirschner wires implanted. Our approach allows to obtain patient specific templates that does not require the complete removal of soft tissue around vertebra. Guide positioning is facilitated thanks to the using of the spinous processes contact point, while false stable positions can be avoided using four redundant contact points. The templates can be used to guide the drill, the insertion of Kirschner in case of use of cannulated screws or to guide directly the screw. After these preliminary ex-vivo animal tests we obtained the authorization of the Italian Health Ministry to start the human study

Summary Statement. Bilaretal epiphysiodesis of he neurocentral cartilages causes shortening of the sagittal length of the pedicles and a subsequent spinal stenosis at the operated segments, resembling that found in patients with achrondroplasia. Introduction. The introduction of pedicle screws in the immature spine may have implications for the growth of the vertebra. The effect of blocking the growth of neurocentral cartilage (NC) is not yet fully defined. Block hypothetically leads to a bilateral symmetrical alteration of the vertebral growth. Using an experimental animal model, our goal is to analyze if a bilateral epiphysiodesis of the NC using pedicle screws is able to induce narrowing of the spinal canal in the thoracolumbar spine. Experimental animals and Methods. A total of 24 domestic pigs were operated on by bilateral blocking of the NC using pedicle screws. The animals were divided into 4 groups depending on the level of blockage: A, low thoracic levels; B, thoracolumbar transitional hinge; C, upper lumbar spine; and D, blocking of the caudal lumbar level below L5 segment. Different morphological, morphometric and standard radiological parameters were analyzed at the thoracic and lumbar vertebrae of the animals. The deviation from the physiological parameters was established by comparing all parameters obtained in the NC-blocked animals with those acquired in 14 pigs without NC blocking. These animals were considered as the control group. Results. None of the animals that underwent NC epiphysiodesis showed asymmetrical spinal growth inducing deformities in the coronal plane. There was neither rotation nor wedging of the vertebral bodies. Whatever the level involved, NC epiphysiodesis caused shortening of the sagittal length of the pedicles and a subsequent decreasing of the antero-posterior diameter of the spinal canal. These features resulted in a frank spinal stenosis at the operated levels. However, the transverse diameter of the spinal canal was conserved in the coronal plane. In the sagittal plane, blocking of the neurocentral cartilage conditioned a lumbar hyperlordosis with compensatory kyphosis of the upper level to the operated vertebra. Conclusions. Symmetrical growth arresting of neurocentral cartilages induces a narrow spinal canal by decreasing the sagittal diameter similar to that observed in patients with achondroplasia. The most affected structure was the development of the vertebral pedicles

Balloon kyphoplasty (BKP) is a minimally invasive surgical technique used to correct kyphosis and vertebral compression fractures. BKP uses cement to fill a void created by the inflation of a balloon in a vertebra, it can be used as an alternative to vertebroplasty to reduce cement extravasation. Issues such as poor inter digitisation of the cement and the trabecular bone can arise with the BKP method. This can be due to a compacted layer created during the procedure which can cause complications post-surgery. The primary aim of this study was to investigate alternative cement application methods which could improve the mechanical strength of the bone-cement interface. Three alternative methods were investigated, and cylindrical bone-cement specimens were created for all methods (BKP and three alternatives). An important part of this study was to replicate the compacted layer created by the inflation of the balloon tamp in BKP. Synthetic trabecular bone specimens (Sawbones®, Pacific Research Laboratories, Vashon Island, Washington, USA) were pre-loaded in compression and the resultant compacted layers were found to replicate the compacted layers found in surgery. Mechanical testing was carried out with an MTS Model 858 Bionix. ®. Servohydraulic load frame using static tensile and torsion loads. Static tests revealed that two of the three alternative methods were an improvement on BKP, with a high statistical significance in relation to the mechanical performance of the bone-cement interface (P < 0.001). This data illustrates the potential to improve the standard BKP technique, in terms of bone-cement interface performance

The aim of this biomechanical study was to investigate the role of the dorsal vertebral cortex in transpedicular screw fixation. Moss transpedicular screws were introduced into both pedicles of each vertebra in 25 human cadaver vertebrae. The dorsal vertebral cortex and subcortical bone corresponding to the entrance site of the screw were removed on one side and preserved on the other. Biomechanical testing showed that the mean peak pull-out strength for the inserted screws, following removal of the dorsal cortex, was 956.16 N. If the dorsal cortex was preserved, the mean peak pullout strength was 1295.64 N. The mean increase was 339.48 N (26.13%; p = 0.033). The bone mineral density correlated positively with peak pull-out strength. Preservation of the dorsal vertebral cortex at the site of insertion of the screw offers a significant increase in peak pull-out strength. This may result from engagement by the final screw threads in the denser bone of the dorsal cortex and the underlying subcortical area. Every effort should be made to preserve the dorsal vertebral cortex during insertion of transpedicular screws

Head collisions in sport can result in catastrophic cervical spine injuries. Musculo-skeletal (MSK) modelling can help analyse the relationship between players' motion, external loading and internal stresses that lead to injury. However, the literature lacks sport specific MSK models. In automotive research the intervertebral disc behaviour has been represented as viscoelastic elements (“bushing”), whose stiffness and damping parameters are often estimated under quasi-static conditions and may lack validity in dynamic impacts. The aim of this study was to develop a validated cervical spine model for axial impacts for future use in the analysis of head-first rugby collisions. A drop test rig was used to replicate a sub-catastrophic axial head impact. A load of 80 N from 0.5 m was applied to the cranial aspect of a C2-C6 porcine spinal specimen mounted in the neutral position. The 3D motion of C3-C5 vertebras (4 kHz) and the cranial axial load (1 MHz) were measured via motion capture (Qualysis, Sweden) and a uniaxial load cell (RDP Electronics Ltd, UK). Specimen specific models were created in NMSBuilder and OpenSim after the vertebrae geometries were obtained from the segmentation of micro-CT images of the specimens. The compressive viscoelastic properties of four vertebral joints (C2-C3 through to C5-C6) were optimised via a Genetic Algorithm (MATLAB v2016b, The Mathworks Inc) to minimise tracking errors. The optimisation converged to a solution of 140–49000 kN/m and 2000–8000 Ns/m for stiffness and damping respectively (RMSE=5.1 mm). Simulated joint displacements ranged between 0.09 – 1.75 mm compared to experimental 0.1 – 0.8 mm. Optimal bushing parameters were higher than previously reported values measured through quasi-static testing. Higher stiffness and damping values could be explained by the higher-dynamics nature of the event analysed related to a different part of the non-linear intervertebral disc load-displacement curve

Summary. Time-lapsed CT offers new opportunities to predict the risk of cement leakage and to evaluate the mechanical effects on a vertebral body by monitoring each incremental injection step in an in-vitro vertebroplasty procedure. Introduction. Vertebroplasty has been shown to reinforce weak vertebral bodies and to prophylactically reduce fracture risks. However, bone cement leakage is a major vertebroplasty related problem which can cause severe complications. Leakage risk can be minimised by injecting less cement into the vertebral body, inevitably compromising the mechanical properties of the augmented bone, as a proper endplate-to-endplate connection of the injected cement is needed to obtain a mechanical benefit. Thus the cement flow in a vertebroplasty procedure requires a better understanding. This study aimed at developing a method to monitor the cement flow in a vertebral body and its mechanical effect. Materials and Methods. Eight fresh frozen human cadaveric vertebrae were prepared for augmentation by performing a bitrans- or bipedicular approach. Following they were XTremeCT-scanned (Scanco, Switzerland) at a nominal resolution of 82µm. A custom made setup enabled to fix the vertebrae in the CT bore (Siemens Emotion6) centrically. Bone cement (Vertecem V+, Synthes GmbH, Switzerland) was injected monopedicularly via a syringe driver (Harvard Apparatus, USA). Injection forces were recorded through a load cell (Type 9211, Kistler Instrumente AG, Switzerland) placed on the driver. Either a custom PEEK cannula or a trocar was inserted into each pedicle of a vertebra to allow artifact-free CT scanning. After each milliliter of injection a CT scan of the vertebra was performed at a nominal resolution of 0.63mm. Subsequently, the CT images were resampled to the original XTremeCT image and the cement cloud was segmented. The image data were then further processed for micro finite element (microFE) modeling (FAIM, Numerics88, Canada). The models were then solved for axial stiffness and Von Mises Stress (VMS) distribution. Finally, the vertebrae underwent a biomechanical quasistatic axial compression test (Mini Bionix II 858, MTS Systems Corp., USA). Results. Endplate-to-endplate connection of the cement was reached in 4 vertebrae. The average volume needed to reach the connection was 5.0±1.2 ml. Cement leakage occurred in all vertebrae, whereby in 4 cases the cement leaked into the spine channel. Each successive cement injection step was characterised with an increase of peak injection forces (16.5±12.7N at 1ml to 70.82±21.14N at 6ml). With respect to axial stiffness the mechanical tests and the microFE models correlated well (R. 2. = 0.778). Analyzing the top 100 VMS an elevated stress concentration between the endplate and the cement was observed unless the endplate was in direct contact with the cement. Conclusion. Cement flow can be monitored precisely at each injection step using the time-lapsed CT approach. Combined with microFE modeling the mechanical properties of the augmented bone can be evaluated for different incremental cement volumes injected. Our results suggest augmenting the bone until an endplate-to-endplate connection is established as otherwise partial filling would increase the risk of failure in the trabecular bone structure. This is in close agreement to other studies

Summary Statement. Burst fractures were simulated in vitro on human cadaveric spine segments. Displacement of the facet joints and pedicles were measured throughout the fracture process showing how these bony structures behave when an impact load is delivered. Introduction. Burst fractures account for almost 30% of all spinal injuries, which may result in severe neurological deficit, spinal instability and hence life impairment. 1. The onset of the fracture is usually traumatic, caused by a high-energy impact loading. Comminution of the endplates and vertebral body, retropulsion of fragments within the canal and increase of the intrapedicular distance are typical indicators of the injury. Experimental and numerical studies have reported strain concentration at the base of the pedicles, suggesting that the posterior processes play a fundamental role in the fracture initiation. 2,3. However, little is known about the dynamic behaviour of the vertebra undergoing an impact load. The aim of this study was to provide an in vitro cadaveric investigation on burst fracture, focusing on the widening of the facet joints and pedicles during the fracture development. Methods. Eight three-adjacent-vertebrae segments (T9-T10-T11, T12-L1-L2, L3-L4-L5) were harvested from three human spines preserving the ligaments and intervertebral discs. A testing frame was designed to hold the sample whilst undergoing an axial impact load (delivered through a drop-weight rig). Lateral displacement was recorded by two transducers (LVDT) sampled at 5000 Hz and data were used to calculate the percent maximum dynamic widening (MW) and percent residual widening after the impact (RW). LVDTs were positioned in contact with the most lateral region of the cranial facet joints where the central vertebra was lumbar; or posteriorly to the base of the pedicles for thoracic. Samples were divided into two groups to achieve two different grade of severity of the fracture by delivering two different amount of energy: High (HE) and Low (LE). Samples underwent HR-pQCT scanning prior and after fracturing to assess percent canal narrowing (CN), intrapedicular distance and grade the fracture. Differences between results were assessed using Mann-Whitney U test. Results. Burst fractures were induced in all the samples (fragment retropulsion was present in all HE samples). The median energy delivered to each group was 206J (HE) and 148J (LE) which led to a significant difference in the median CN (HE: 32.4%; LE: 11.8%; p=0.029). No significant difference was found between HE and LE in terms of MW (p=0.11), or RW (p=0.85). Furthermore, MW and CN were poorly correlated (R. 2. =0.13). In all the cases, the first peak in the widening data coincided with MW (median 12.8%, range 4.3–21.8%). RW measurements (median 2.8%, range −1.3–11.5%) were validated against HR-pQCT scans showing excellent agreement (R. 2. =0.93). Discussion/Conclusion. Results from this study provided further insight on the burst fracture process supporting the wedging effect of the adjacent facet joints when the impact load is transmitted. Indeed, the pedicles were forced to widen up to a critical value (MW), after which they fractured. Further experiments will help clarifying the influence of the amount of energy delivered

Summary Statement. From a mechanical point of view, the clinical use of pedicle screws in the atlas is a promising alternative to lateral mass screws due to an increased biomechanical fixation. Introduction. The most established surgical technique for posterior screw fixation in the atlas (C1) is realised by screw placement through the lateral mass [1]. This surgical placement may lead to extended bleeding from the paravertebral venous plexus as well as a violation of the axis (C2) nerve roots [1]. Using pedicle screws is an emerging technique which utilises the canal passing through the posterior arch enabling the use of longer screws with a greater contact area while avoiding the venous plexus and axis nerve roots. The aim of this ex vivo study was to investigate if pedicle screws in C1 bear the potential to replace the more common lateral mass screws. Therefore, the comparative biomechanical fixation strengths in terms of cycles to failure, stiffness, and removal torque were investigated. Methods. Nine C1 cadaveric vertebrae from donors aged 58.0 ± 11.1 years were separated, CT scanned (Mx8000 IDT 16, Philips Healthcare, DA Best, The NL) with a phantom, and stored at −22°C. Each vertebra received one lateral mass screw and one pedicle screw of the same size (diameter: 3.5 mm, length: 26 mm, Synapse System, Synthes GmbH, Oberdorf, CH). The side on which each screw was placed into the vertebra was allocated based on BMD, age, gender, and testing order. Depending on the surgical technique the entry point varied; the pedicle screw entered through the posterior arch, and the lateral mass screw was inserted further inferior through the lateral mass. The screw tips converged to the same height and depth. Specimens were subjected to a sinusoidal, cyclic (0.5 Hz) fatigue loading at the screw head (858 Bionix®, MTS, Eden Prairie, MN). The peak compressive and tensile forces started from ±15 N and increased by 0.05 N every cycle. Testing was stopped at 5 mm displacement. Cycles to failure, displacement, initial and final cyclic stiffness were measured. After fatigue testing a surgeon evaluated each screw by hand for looseness. Final CT scans were taken and then the removal torque was measured. Results. The specimens were of normal bone quality (BMD = 226 ± 69.0 mgHA/cm. 3. ). The pedicle screw technique consistently and significantly out-performed the lateral mass technique in cycles to failure (p=0.001, r. 2. =0.48), initial stiffness (p=0.01, r. 2. =0.29), end stiffness (p=0.005, r. 2. =0.18), and removal torque (∗p=0.05, r. 2. =0.18). After testing only 33% of pedicle screws were loose compared to 100% of lateral mass screws. Discussion. Utilizing the C1 posterior arch, the pedicle screws were able to withstand a 32% higher toggle force than the lateral mass screws while maintaining a higher stiffness throughout and after testing. The advantages likely arise due to an increased depth into the bone and the smaller canal width. Due to the fixation benefits in the atlas, the clinical use of pedicle screws is a promising alternative to lateral mass screws. Funding from the State of Hamburg and the Marie Curie ITN project, SpineFX, is kindly acknowledged. The authors thank Synthes GmbH for providing the screws

Summary Statement. It is now possible to diagnose osteoporosis using incidental abdominal CT scans; applying this approach to fractures of the cervical spine demonstrates levels of osteoporosis in patients over 65. Introduction. Recently published data now makes it possible to screen for osteoporosis in patients who, in the course of their hospital stay, have had Computed Tomography (CT) scans of their abdomen for reasons other than direct imaging. This is as a result of CT derived bone mineral density (BMD) in the first lumbar vertebra (L1) being correlated BMD derived from Dual-energy X-ray absorptiometry (DEXA) scans. The advantage of this is the reduction in both cost and radiation exposure. Although age has a detrimental effect on BMD, relatively few patients have formal DEXA studies. The aims of this study were to evaluate the utility of this new technique in a cohort of patients with acute fractures of the cervical spine and to compare relative values for BMD in patients aged over 65 with those aged under 65, and thus define the role of osteoporosis in these injuries. Methods & Patients. Following Institutional review board approval, we performed a retrospective study of patients who presented to a level I trauma center with acute fractures of the cervical spine between 2010 and 2013; patients also had to have had a CT scan of their L1 vertebra either during the admission or within 6 months of their admission (for any other clinical reason). Using a picture archiving and communication (PACS) system, we generated regions of interest (ROI) of similar size in the body of L1 (excluding the cortex), in line with the publication by Pickhardt et al., and computed the mean values for Hounsfield units (HU). These values were compared against established threshold values which differentiate between osteoporosis and osteopenia; for a balanced sensitivity and specificity, <135 HU is the threshold and for 90% sensitivity a HU threshold of <160 HU is set. Comparisons were also performed between age stratified groups. Results. A total of 187 patients were reviewed for eligibility, 91 patients met the criteria with 53 patients aged 64 years or younger (range 23–64) and 38 patients aged above 65 years (range 65–98). In the younger cohort, 6/53 (11% were osteoporotic, using the lower threshold, while the higher threshold indicated 5/53 (17%) of patients under 65 years were osteoporotic; mean HU for the group was 195.8 (SD 43.3). In the older cohort, 24/38 (63%) were osteoporotic using the lower threshold, whereas 34/38 (89%) were osteoporotic using the higher threshold. Mean HU for the cohort aged over 65 years was 118.7 (SD 38.4). Age based comparison of the mean values, regardless of threshold, was statistically significant (p<0.001) in both cases. Discussion and Conclusions. This study demonstrates, for the first time in the cervical spine (including C2), the role of age related osteoporosis in acute fractures of the cervical spine. This new technique harnessing the presence of opportunistic CT scans of the abdomen saves on the extra cost and radiation exposure that may be associated with DEXA scanning. In younger patients, the higher threshold indicated 17% were osteoporotic – in the setting of an opportunistic scan, this may afford them the opportunity to commence prophylactic treatment to prevent future fractures. We believe these result have the potential to significantly impact future clinical practice

Clinical investigations show that the cervical spine presents wide inter-individual variability, where its motion patterns and load sharing strongly depend on the anatomy. The magnitude and scope of cervical diseases, including disc degeneration, stenosis, and spondylolisthesis, constitute serious health and socioeconomic challenges that continue to increase along with the world”s growing aging population. Although complex exact finite element (FE) modeling is feasible and reliable for biomechanical studies, its clinical application has been limited as it is time-consuming and constrained to the input geometry, typically based on one or few subjects. The objective of this study was twofold: first to develop a validated parametric subject-specific FE model that automatically updates the geometry of the lower cervical spine based on different individuals; and second to investigate the motion patterns and biomechanics associated with typical cervical spine diseases. Six healthy volunteers participated in this study upon informed consent. 26 parameters were identified and measured for each vertebra in the lower cervical spine from Lateral and AP radiographs in neutral, flexion and extension viewpoints in the standing position. The lower cervical FE model was developed including the typical vertebrae (C3-C7), intervertebral discs, facet joints, and ligaments using ANSYS (PA, USA). In order to validate the FE model, the bottom surface of C7 was fixed, and a 73.6N preload together with a 1.8 N.m pure moment were input into the model in both flexion and extension. The results were compared to experimental studies from literature. Disc degeneration disease (DDD) was used as an example, where the geometry of C5-C6 disc was changed in the model to simulate 3 different grades of disc degeneration (mimicking grades 1 to 3), and the resulting biomechanical responses were evaluated. The average ranges of motion (ROM) were found to be 4.84 (±0.73) degrees and 5.36 (±0.68) degrees for flexion and extension for C5-C6 functional unit, respectively, in alignment with literature. The total ROM of the model with disc generation grades 2 and 3 was found to have decreased significantly as compared to the intact model. In contrast, the axial stresses on the degenerated discs were significantly higher than the intact discs for all 3 degeneration grades. Our preliminary results show that this novel validated subject-specific FE model provides a potential valuable tool for noninvasive time and cost effective analyses of cervical spine biomechanical (kinematic and kinetic) changes associated with various diseases. The model also provides an opportunity for clinicians to use quantitative data towards subject-specific informed therapy and surgical planning. Ongoing and future work includes expanding the studied population to investigate individuals with different cervical spine afflictions

DVC allowed measurements of displacement and strain distribution in bone through the comparison of two, or more, 3D images. Hence, it has a potential as a diagnostic tool in combination with clinical CT. Currently, traditional computed tomography (CT) allows for a detailed 3D analysis of hard tissues, but imaging in a weight-bearing condition is still limited. PedCAT-CT (Curvebeam, USA) emerged as a novel technology allowing, for the first time, 3D imaging under full-weight bearing (Richter, Zech et al. 2015). Specifically, a PedCAT-CT based DVC was employed to establish its reliability through the strain uncertainties produced on bone structure targets, preliminarily to any further clinical studies. In addition, a reverse engineering FE modeling was used to predict possible force associated to displacement errors from DVC. Three porcine thoracic vertebrae were used as bone benchmark for the DVC (Palanca, Tozzi et al. 2016, Tozzi, Dall'Ara et al. 2016). The choice of using porcine vertebrae (in a CT designed for foot/ankle) was driven by availability, as well as similar dimensions to the calcaneus. Each vertebra was immersed in saline solution and scanned twice without any repositioning (zero-strain-test) with a pedCAT-CT (Curvebeam, USA) obtaining an isotropic voxel size of 370 micrometers. Volumes of interest of 35 voxel were cropped inside the vertebrae. Displacement and strains were evaluated using DVC (DaVis-DC, LaVision, Germany), with different spatial resolution. The displacement maps were used to predict the force uncertainties via FE (Ansys Mechanical v.14, Ansys Inc, Canonsburg, PA). Each element was assigned a linear elastic isotropic constitutive law (Young modulus: 8 GPa, Poisson's ratio: 0.3, as in (Follet, Peyrin et al. 2007)). Overall, the precision error of strain measurement was evaluated as the average of the standard deviation of the absolute value of the different component of strain (Liu and Morgan 2007). The force uncertainties obtained with the FE analysis produced magnitudes ranging from 231 to 2376 N. No clear trend on the force was observed in relation to the spatial resolution. Precision errors were smaller than 1000 microstrain in all cases, with the lowest ranging from 83 microstrain for the largest spatial resolution. Full-field strain on the bone tissue did not seem to highlight a preferential distribution of error in the volume. The precision errors showed that the pedCAT-CT based DVC can be sufficient to investigate the bone tissue failure (7000–10000 microstrain) or, physiological deformation if well-optimized. FE analysis produced important force uncertainties up to 2376 N. However, this is a preliminary investigation. Further investigation will give a clearer indication on DVC based PedCAT-CT, as well as force uncertainties predicted. So far, the DVC showed its ability to measure displacement and strain with reasonable reliability with clinical-CT as well

Prophylactic augmentation is meant to reinforce the vertebral body (VB), but in some cases it is suspected to actually weaken it. To elucidate the biomechanical efficacy of prophylactic augmentation, the full-field three-dimensional strain distributions were measured for the first time inside prophylactic-augmented vertebrae. Twelve thoracic porcine vertebrae were assigned to three groups: 4 were augmented with bone cement for vertebroplasty (Mendec-Spine, Tecres), 4 were treated with another bone cement for vertebroplasty (Calcemex-Spine, Tecres) while the other 4 were tested untreated as a control. Destructive tests were carried out under axial compression, in a step-wise fashion (unloaded, 5%, 10% and 15% compression). At each loading step, μCT-images were acquired. The internal strain distribution was investigated by means of DVC analysis. Some augmented specimens were stronger than the respective control, while others were weaker. In most of the specimens, the strain distribution in the elastic regime (5% compression) seemed to predict the location of the micro-damage initiation before it actually became identifiable (at 10% and 15% compression). The measured strain had the same order of magnitude for all groups. However, in the control vertebrae, the highest strain would unpredictably appear at any location inside the VB. Conversely, for both augmentation groups, the highest strains were measured in the regions adjacent to the injected cement mass, whereas the cement-interdigitated-bone was less strained. Localization of high strains and failure was consistent between specimens, but different between the two cement types: with Mendec-Spine failure the highest strains were mainly localized at mid-height and at the same level where the cement mass was localized; with Calcemex-Spine failure the highest strains were mainly cranial and caudal to the cement mass. Both the micro-CT images, and the DVC strain analysis highlighted that:. The cement mass was less strained than any other regions in the vertebra. Failure never started inside the cement mass. This can be explained with the additional stiffening and reinforcement associated with the infiltration of the cement inside the trabecular bone. The highest strains and failure were localized in the bone adjacent to the cement-bone interdigitated region. This can be explained by the strain concentration between the cement-interdigitated bone (stiffer and stronger), and the adjacent non-augmented trabecular bone. The strain maps in the elastic regime and the localization of failure was different in the augmented vertebrae, when compared to the natural controls. This suggests an alteration of the load sharing in the augmented structure where the load is mostly carried by the cement region. The different localization of failure initiation between the two augmented groups could be explained by the different mechanical properties of the two cements. This study has demonstrated the potential of DVC in measuring the internal strain and failure in prophylactic-augmented vertebrae. It has been shown that failure starts inside the augmented VB, next to the injected cement mass. This can help establishing better criteria (in terms of localization of the cement mass) in order to improve clinical protocols for vertebroplasty surgical procedures