Aims. Scapular
Aims. The aim of this study was to analyze the association between the shape of the distal radius sigmoid
This study provides recommendations on the position
of the implant in reverse shoulder replacement in order to minimise
scapular
The condylopatellar
We hypothesised that an independent
Aims. The aims of this study were: firstly, to investigate the influence
of the thickness of cartilage at the sigmoid
Purpose: We hypothesised that independent
The shape of the glenoid can vary between pear and oval, depending on the presence of a glenoid
Multiple myeloma (MM) is an incurable hematological tumor stemming from malignant plasma cells. MM cells accumulate in the bone marrow (BM) and shape the BM niche by establishing complex interactions with normal BM cells, boosting osteoclasts (OCLs) differentiation and causing bone disease. This unbalance in bone resorption promotes tumor survival and the development of drug resistance. The communication between tumor cells and stromal cells may be mediated by: 1) direct cell-cell contact; 2) secretion of soluble factors, i.e. chemokines and growth factors; 3) release of extracellular vesicles/exosomes (EVs) which are able to deliver mRNAs, miRNAs, proteins and metabolites in different body district. Primary CD138+ MM cells were isolated from patients BM aspirates. MM cell lines were cultured alone in complete RPMI-1640 medium or co-cultured with murine (NIH3T3) or human (HS5) BMSC cell lines or murine Raw264.7 monocytes in DMEM medium supplemented with 10% V/V FBS. Silencing of Jagged1 and Jagged2 was obtained by transient expression of specific siRNAs or by lentiviral transduction using a Dox-inducible system (pTRIPZ). EVs were isolated using differential ultracentrifugation. EVs concentration and size were analyzed using Nano Track Analysis (NTA) system. The uptake of PKH26-labelled MM-derived EVs by HS5 or Raw264.7 was measured after 48 hours by confocal microscopy and flow cytometry. Osteoclast (OCL) differentiation of Raw264.7 cells was induced by 50ng/ml mRANKL, co-culturing with MM cells, CM or EVs. OCLs were stained by TRAP Kit and counted. Bone resorption was assessed by Osteo Assay Surface plates. Flow cytometric detection of apoptotic cells was performed after staining with Annexin V. Gene expression was analyzed by qRT-PCR, while protein levels were determined using flow cytometry ELISA or WB.
We have investigated whether shape of the knee
can predict the clinical outcome of patients after an anterior cruciate
ligament rupture. We used statistical shape modelling to measure
the shape of the knee joint of 182 prospectively followed patients
on lateral and Rosenberg view radiographs of the knee after a rupture
of the anterior cruciate ligament. Subsequently, we associated knee
shape with the International Knee Documentation Committee subjective
score at two years follow-up. The mean age of patients was 31 years
(21 to 51), the majority were male (n = 121) and treated operatively
(n = 135). We found two modes (shape variations) that were significantly
associated with the subjective score at two years: one for the operatively
treated group (p = 0.002) and one for the non-operatively treated
group (p = 0.003). Operatively treated patients who had higher subjective
scores had a smaller intercondylar
Objective. In this study, we aim to compare total bone amount extracted in total knee arthroplasty in implant design and the bone amount extracted through intercondylar femoral
We undertook a study on eight arms from fresh cadavers to define the clinical usefulness of the lesser sigmoid
Purpose: This study was conducted to analyse the importance of the Malgaigne
Background: The orientation and the position of glenoid are two factors which have been pointed out as a cause of
Introduction:. Successful total joint arthroplasty requires accruate and reproducible acetabular component position. Acetabular component malposition has been associated with complications inlcuding dislocation, implant loosening, and increased wear. Recent literature had demonstrated that high-volume fellowship trained arthroplasty surgeons are in the “safe zone” for cup inclination and anteversion only 47% of the time. (1) Computer navigation has improved accuracy and reproducibility but remains expensive and cumbersome to many hospital and physicians. Patient specific instrumentation (PSI) has been shown to be effective and efficient in total knee replacements. The purpose of this study was to determine in a cadaveric model the anteversion and inclination accuracy of acetabular guides compared to a pre-operitive plan. Methods:. 8 fresh-frozen cadaveric pelvis specimens underwent Computer Tomography (CT) in order to create a 3D reconstruction of the acetabulum. Based on these 3D reconstruction, a pre-operative plan was made positioning the patient specific acetabulum guides at 40 degrees of inclination and 20 degrees of anteversion in the pelvis.(Figure 1) The guides were created based on the specific bony morphology of the acetabular
It has been suggested that an increased posterior
tibial slope (PTS) and a narrow
We present simple but effective retractors used in pairs to expose the sciatic
In the first few months of life, a
Purpose. The purpose of this study was to determine whether intra-operative identification of osseous ridge anatomy (lateral intercondylar “residents” ridge and lateral bifurcate ridge) could be used to reliably define and reconstruct individuals' native femoral ACL attachments in both single-bundle (SB) and double-bundle (DB) cases. Methods. Pre-and Post-operative 3D, surface rendered, CT reconstructions of the lateral intercondylar
We describe five patients, seen since 1984, with posterior shoulder pain and isolated wasting and weakness of the infraspinatus. In four of these a ganglion in the spinoglenoid