Preoperative bone-marrow-derived cell mobilization by G-CSF is a safe orthopaedic procedure and allows circulation in the blood of high numbers of CD34+ve cells, promoting osseointegration of a bone substitute. Granulocyte-colony-stimulating-factor(G-CSF) has been used to improve repair processes in different clinical settings for its role in bone-marrow stem cell(CD34+ and CD34-) mobilization. Recent literature suggests that G-CSF may also play a role in skeletal-tissue repair processes. Aim of the study was to verify the feasibility and safety of preoperative bone-marrow cell (BMC) mobilization by G-CSF in orthopaedic patients and to evaluate G-CSF efficacy in accelerating bone regeneration following opening-wedge high tibial valgus osteotomy(HTVO) for genu varum.Summary Statement
Introduction
Hypoxia enhances chondrocyte phenotype of cells migrating from cartilage fragments, thus supporting the use of chondral fragment as a potential cell source for one-stage cartilage repair Minced cartilage fragments are a viable cell source for one stage cartilage repair, as shown in both in preclinical and clinical studies. However, the joint microenvironment, in which the repair process takes place, is hypoxic and no evidences are present in literature regarding the behaviour of cartilage fragments in a hypoxic environment. Aim of the study is to verify if hypoxia could influence chondrocyte outgrowth from cartilage fragments into a Hyaluronic-Acid/fibrin scaffold and evaluate its effects on migrating chondrocyte behaviour, compared to normoxic condition. This could be significant in the perspective of a wide clinical application of human chondral fragments for single stage repair.Summary Statement
Introduction
Aim of study is to verify feasibility of peroneal tendoscopy and to clarify the histological structure of peroneal vincula, so formulating a hypothesis regarding their functional role. Peroneal tendons possess a vascular supply through mesotendineal structures (vincula), previously related to trophic role and healing response; aim of study is to verify feasibility of tendoscopy in evaluating peroneal tendon and vincula and to clarify histological structure of vincula and presence of nervous tissue, so formulating a hypothesis regarding their functional role. Cadaver study was performed on 8 fresh-frozen ankles, verifying accessibility of endoscope to tendon and vincula; samples from cadaveric vincula were taken; 5 peroneal vincula biopsies were obtained from 5 patients affected by ankle instability, undergoing tendoscopy for chronic lateral ankle pain. Tendoscopy was performed for persistent pain at the posterior margin of lateral malleolus after at least 4 months of nonoperative treatment. Biopsies were taken from center of pathologic vincula. Patients biopsies and cadaveric samples were analyzed with light microscopy and immunohistochemistry (anti-humanS100antibody) Peroneal tendons are accessible by endoscope along whole common sheath; vincula were found in all cadaveric specimens; intraoperative finding of vincula lesion (thickening/scarring) was found in all patients biopsies. Histology and immunohistochemistry revealed presence of nervous fibers inside the intimate structure of peroneal vincula both in cadaveric specimens and in patients biopsies. Tendoscopy as a useful tool in visualizing the entire length of peroneal tendons, allowing the surgeon to diagnose and treat different peroneal disorders. Although literature provides no data about innervations of peroneal vincula, presence of free nervous fibers inside vincula structure is consistent with a proprioceptive role of the vinculum in peroneal tendon physiology. Moreover, our findings in patients biopsies suggest lesion of peroneal vinculum is a nociceptive source and an important element leading, synergistically with other soft tissues (i.e. joint capsule, lateral ligaments) injuries, to proprioception impairment in clinical pictures of chronic ankle instability. So selective excision of degenerated areas of vincula can be justified as accessory procedure in treatment of chronic lateral pain in patients affected by chronic ankle instability.
This study reports the 5-year clinical and radiological outcomes of a simple arthroscopic-assisted technique for Schatzker type II and III tibial plateau fractures, without bone grafting. Forty six patients (46% males, 54% females, average age 48 years, SD 13.6 years), with tibial plateau fractures Schatzker type II (41%) and III (59%), underwent an arthroscopic-assisted technique conceived to use a compacted cancellous bone graft, taken from the medial metaphyseal side of the tibia, and a percutaneous fixation. The patients were prospectively followed-up at one, three and five years from surgery. Independent assessments were carried out using Knee Society Score, HSS score and Rasmussen’s clinical and radiological scores. At 5-year follow-up patients underwent a weight-bearing radiograph of both limbs. At last follow-up evaluation Knee Score (average 93.2, SD 7.7) was excellent in 37 patients (80%), good in six (13%), fair in three (7%). Function Score (average 94.8, SD 8.51) was excellent in 38 patients (83%), good in five (11%), fair in three (6%). HSS score (average 93.4, SD 8.23) was excellent in 41 patients (89%), good in five (11%). The average Rasmussen clinical score was 28.2 (SD 1.4). The radiological Rasmussen score was excellent in five patients (11%), good in 39 (85%) and fair in two (4%). In the weight-bearing radiographs a valgus deviation was present in four patients (8.7%). Arthroscopic-assisted technique for lateral tibial plateau fractures without bone grafting has outcomes encouraging and comparable to the results of other techniques that use either iliac crest graft or bone substitutes.
