Background

Previously, we demonstrated the effectiveness of phase symmetry (PS) features for segmentation and localisation of bone fractures in 3D ultrasound for the purpose of orthopedic fracture reduction surgery. We recently proposed a novel real-time image-processing method of bone surface extraction from local phase features of clinical 3D B-mode ultrasound data. We are presenting a computational study and outline planned future developments for integration into a computer aided orthopedic surgery framework.

Previously, we demonstrated the effectiveness of phase symmetry (PS) features for segmentation and localisation of bone fractures in 3D ultrasound for the purpose of orthopaedic fracture reduction surgery. We recently proposed a novel real-time image-processing method of bone surface extraction from local phase features of clinical 3D B-mode ultrasound data. We are presenting a computational study and outline of planned future developments for integration into a computer aided orthopaedic surgery framework.

Our image-processing pipeline was implemented on three platforms: (1) using an existing PS extraction C++ algorithm on a dual processor machine with two Xeon x5472 CPUs @ 3GHz with 8GB of RAM, (2) using our proposed method implemented in MATLAB running on the same machine as in (1), and (3) CUDA implementation of our method implemented on a professional GPU (Nvidia Tesla c2050).

We ran these three implementations 20 times each on 128×128×128 scans of the iliac crest in live subjects and repeated the processing for 15 combinations of filter parameters. On average, the C++ implementation took 1.93s per volume, the MATLAB implementation 1.28s, and the GPU implementation 0.08s. Overall, our GPU implementation is between 15 and 25 times faster than the state-of-the-art method.

Implementing our algorithm on a professional grade GPU produced dramatic computational improvements, enabling full 3D datasets to be processed in an average time of under 100ms, which, if proven in a clinical system, would allow for near real time computation. We are currently implementing our algorithm on an open research sonography platform (Ultrasonix Medical Corporation). High-powered graphic cards can easily be integrated into the open architecture of this system, thus enabling GPU computation on diagnostic medical and research ultrasound devices.

We intend to use this platform within a surgical environment for accurate and automatic detection of fractures and as an integral part of our developing computer aided surgery pipeline, in which we use PS features to register intra-operative ultrasound to pre-operative computed tomography images.

Shoulder septic arthritis is uncommon and frequently misdiagnosed, resulting in severe consequences. This study evaluated the demographics, bacteriological profile, antibiotic susceptibility, treatment regimens, and clinical outcomes. This is a 10-year retrospective observational analysis of 30 patients (20 males and 10 females) who were treated for septic arthritis of the shoulder. The data collecting process utilised clinical records, laboratory archives, and x-ray archives. We gathered demographic information, pre- and post-intervention clinical data, serum biochemical markers, and the results of imaging examinations. All patients had a surgical arthrotomy and joint debridement in the operating room, and specimens were taken for culture and sensitivity testing. The specimens were cultivated for at least seventy-two hours. Shoulder joint ranges of motion, comorbidities, and the presence of osteomyelitis were assessed clinically to determine the outcome. All statistical analyses were conducted using the STATA 17 statistical software. Analysis of correlation between categorical variables was performed using the chi-squared test. The majority of the study patients were black Africans (97%). The age range of the group was from 8 days to 17 years. At presentation, 33% of patients had a low-grade fever, whereas the majority (60%) had normal body temperature. The average length of symptoms was 3.9 days (ranged from 1 day to 15 days), and the majority of patients had an increased white cell count (83%) and C-reactive protein (98%). There was accumulation of fluid in the joint of all individuals who received shoulder ultrasound imaging. We noted a significant incidence of gram-positive cocci, which were mostly susceptible to first-line antibiotics. Shoulder stiffness affected 63% of patients and chronic osteomyelitis affected 50% of individuals. Neither the severity nor the duration of the symptoms was related to an increased risk of osteomyelitis. The results of this study revealed that the clinical characteristics and bacterial profile of septic arthritis of the shoulder conform to typical patterns. The likelihood of osteomyelitis and an unfavourable prognosis is considerable

Aims. The aim of this study was to establish a reliable method for producing 3D reconstruction of sonographic callus. Methods. A cohort of ten closed tibial shaft fractures managed with intramedullary nailing underwent ultrasound scanning at two, six, and 12 weeks post-surgery. Ultrasound capture was performed using infrared tracking technology to map each image to a 3D lattice. Using echo intensity, semi-automated mapping was performed to produce an anatomical 3D representation of the fracture site. Two reviewers independently performed 3D reconstructions and kappa coefficient was used to determine agreement. A further validation study was undertaken with ten reviewers to estimate the clinical application of this imaging technique using the intraclass correlation coefficient (ICC). Results. Nine of the ten patients achieved union at six months. At six weeks, seven patients had bridging callus of ≥ one cortex on the 3D reconstruction and when present all achieved union. Compared to six-week radiographs, no bridging callus was present in any patient. Of the three patients lacking sonographic bridging callus, one went onto a nonunion (77.8% sensitive and 100% specific to predict union). At 12 weeks, nine patients had bridging callus at ≥ one cortex on 3D reconstruction (100%-sensitive and 100%-specific to predict union). Presence of sonographic bridging callus on 3D reconstruction demonstrated excellent reviewer agreement on ICC at 0.87 (95% confidence interval 0.74 to 0.96). Conclusion. 3D fracture reconstruction can be created using multiple ultrasound images in order to evaluate the presence of bridging callus. This imaging modality has the potential to enhance the usability and accuracy of identification of early fracture healing. Cite this article: Bone Joint Res 2021;10(12):759–766

Introduction. We aim to assess whether radiographic characteristics of the greater tuberosity fragment can predict rotator cuff tears inpatients with anterior shoulder dislocations combined with an isolated fracture of the greater tuberosity. Methods. A retrospective single-centre case series of 61 consecutive patients that presented with anterior shoulder dislocations combined with an isolated fracture of the greater tuberosity between January 2018 and July 2022. Inclusion criteria: patients with atraumatic anterior shoulder dislocation associated with an isolated fracture of the greater tuberosity with a minimum follow-up of 3-months. Exclusion criteria: patients with other fractures of the proximal humerus or glenoid. Rotator cuff tears were diagnosed using magnetic resonance or ultrasound imaging. Greater tuberosity fragment size and displacement was calculated on plain radiographs using validated methods. Results. The case series was composed of 22 men and 39 women with a mean age of 65 years (29 - 91 years). The mean follow-up was 15months and median follow up 8.5 months (3 – 60 months). A rotator cuff tear was diagnosed in 14 patients (16%) and involved the supraspinatus (13), infraspinatus (4) and subscapularis (2). Full-thickness tears occurred in 6 patients and partial-thickness tears in 8patients. The mean time from initial injury to rotator cuff tear diagnosis was 5 months (2 – 22 months). The mean greater tuberosity fragment length was 23.4 mm in rotator cuff tear patients versus 32.6 mm in those without a tear (p = 0.006, CI: -15 - -2). The mean greater tuberosity. fragment width was 11.1 mm in rotator cuff tear patients versus 17.8 mm in those without a tear (p = 0.0004, CI: -10 - -2). There was no significant difference in the super inferior and anteroposterior fragment displacement between the two groups. Conclusion. In patients with shoulder dislocations combined with an isolated fracture of the greater tuberosity, rotator cuff tears are associated with a smaller sized greater tuberosity fragment

A challenging problem in ultrasound based orthopaedic surgery is the identification and interpretation of bone surfaces. Recently we have proposed a new fully automatic ultrasound bone surface enhancement filter in the context of spine interventions. The method is based on the use of a Gradient Energy Tensor filter to construct a new feature enhancement metric, which we call the Local Phase Tensor. The goal of this study is to provide further improvements to the proposed filtering method by incorporating a-priori knowledge about the physics of ultrasound imaging and salient grouping of enhanced bone features. Typical ultrasound scan of the spine, there is a large soft tissue interface present close to the transducer surface with high intensity values similar to those of the bone anatomy response. Typical ultrasound image segmentation or enhancement methods will be affected by this thick soft tissue response. In order to weaken this soft tissue interface we calculate a new transmission map where features deeper in the ultrasound image have higher transmission values and shallow features have lower transmission values. The calculation of this new US transmission/attenuation map allows the proposed image enhancement method to mask out erroneous regions, such as the soft tissue interface, and improve the accuracy and robustness of the spine surface enhancement. The masked US images were used as an input to the LPT image enhancement method. In order to provide a more compact spine surface representation and further reduce the typical US imaging artifacts and soft tissue interfaces we calculate saliency Local Phase Tensor features. The saliency images are computed using Difference of Gaussian filters. Qualitative results, obtained from in vivo clinical scans, show a strong correspondence between enhanced features and the actual bone surfaces present in the ultrasound scans. Future work will include the extension of the proposed method to 3D and validation of the method in the context of intra-operative ultrasound image registration in CAOS applications

The opposable thumb is one of the defining characteristics of human anatomy and is involved in most activities of daily life. Lack of optimal thumb motion results in pain, weakness, and decrease in quality of life. First carpometacarpal (CMC1) osteoarthritis (OA) is one of the most common sites of OA. Current clinical diagnosis and monitoring of CMC1 OA disease are primarily aided by X-ray radiography; however, many studies have reported discrepancies between radiographic evidence of CMC1 OA and patient-related outcomes of pain and disability. Radiographs lack soft-tissue contrast and are insufficient for the detection of early characteristics of OA such as synovitis, which play a key role in CMC OA disease progression. Magnetic resonance imaging (MRI) and two-dimensional ultrasound (2D-US) are alternative options that are excellent for imaging soft tissue pathology. However, MRI has high operating costs and long wait-times, while 2D-US is highly operator dependent and provides 2D images of 3D anatomical structures. Three-dimensional ultrasound imaging may be an option to address the clinical need for a rapid and safe point of care imaging device. The purpose of this research project is to validate the use of mechanically translated 3D-US in CMC OA patients to assess the measurement capabilities of the device in a clinically diverse population in comparison to MRI. Four CMC1-OA patients were scanned using the 3D-US device, which was attached to a Canon Aplio i700 US machine with a 14L5 linear transducer with a 10MHz operating frequency and 58mm. Complimentary MR images were acquired using a 3.0 T MRI system and LT 3D coronal photon dense cube fat suppression sequence was used. The volume of the synovium was segmented from both 3D-US and MR images by two raters and the measured volumes were compared to find volume percent differences. Paired sample t-test were used to determine any statistically significant differences between the volumetric measurements observed by the raters and in the measurements found using MRI vs. 3D-US. Interclass Correlation Coefficients were used to determine inter- and intra-rater reliability. The mean volume percent difference observed between the two raters for the 3D-US and MRI acquired synovial volumes was 1.77% and 4.76%, respectively. The smallest percent difference in volume found between raters was 0.91% and was from an MR image. A paired sample t-test demonstrated that there was no significant difference between the volumetric values observed between MRI and 3D-US. ICC values of 0.99 and 0.98 for 3D-US and MRI respectively, indicate that there was excellent inter-rater reliability between the two raters. A novel application of a 3D-US acquisition device was evaluated using a CMC OA patient population to determine its clinical feasibility and measurement capabilities in comparison to MRI. As this device is compatible with any commercially available ultrasound machine, it increases its accessibility and ease of use, while proving a method for overcoming some of the limitations associated with radiography, MRI, and 2DUS. 3DUS has the potential to provide clinicians with a tool to quantitatively measure and monitor OA progression at the patient's bedside

The treatment of extremity ballistic injury is challenging in that the zone of injury can be extensive and determining the surgical exposure can be difficult. We describe a method of pre-operative evaluation of the zone of injury in conjunction with the regional anesthesiologist utilizing ultrasound to determine the presence of nerve disruption. This non-invasive method of examination may elucidate whether significant nerve exists and may also serve to pinpoint the location of injury. Such information allows the surgeon to more effectively and efficiently surgically expose the zone of injury and understand the boundaries of the nerve outside the zone of injury. Moreover, such preoperative evaluation may at times obviate the need for exploratory surgery at all. It is important for the anesthesiologist and surgeon to work together with respect to the ability to both interpret the ultrasound images and to clinically correlate the findings. The zone of tissue disruption in ballistic injuries is extremely variable. It is beneficial to both the surgeon and patient to engage in a collaborative effort with an experienced regional anesthesiologist who is well-versed in interpretation of ultrasound images and tissue plane disruption in an effort to minimize surgical time and the potential unintended consequences of unnecessary exploration. We present a series of cases representing instances wherein the zone of injury was small, extensive, and a unique situation in which there was in fact no injury present despite clinical symptoms and MRI consistent with radial nerve disruption

Objectives. The aim of this study was to review the current evidence and future application for the role of diagnostic and therapeutic ultrasound in fracture management. Methods. A review of relevant literature was undertaken, including articles indexed in PubMed with keywords “ultrasound” or “sonography” combined with “diagnosis”, “fracture healing”, “impaired fracture healing”, “nonunion”, “microbiology”, and “fracture-related infection”. Results. The use of ultrasound in musculoskeletal medicine has expanded rapidly over the last two decades, but the diagnostic use in fracture management is not routinely practised. Early studies have shown the potential of ultrasound as a valid alternative to radiographs to diagnose common paediatric fractures, to detect occult injuries in adults, and for rapid detection of long bone fractures in the resuscitation setting. Ultrasound has also been shown to be advantageous in the early identification of impaired fracture healing; with the advent of 3D image processing, there is potential for wider adoption. Detection of implant-related infection can be improved by ultrasound mediated sonication of microbiology samples. The use of therapeutic ultrasound to promote union in the management of acute fractures is currently a controversial topic. However, there is strong in vitro evidence that ultrasound can stimulate a biological effect with potential clinical benefit in established nonunions, which supports the need for further investigation. Conclusion. Modern ultrasound image processing has the potential to replace traditional imaging modalities in several areas of trauma practice, particularly in the early prediction of impaired fracture healing. Further understanding of the therapeutic application of ultrasound is required to understand and identify the use in promoting fracture healing. Cite this article: J. A. Nicholson, S. T. J. Tsang, T. J. MacGillivray, F. Perks, A. H. R. W. Simpson. What is the role of ultrasound in fracture management? Diagnosis and therapeutic potential for fractures, delayed unions, and fracture-related infection. Bone Joint Res 2019;8:304–312. DOI: 10.1302/2046-3758.87.BJR-2018-0215.R2

Sonographic callus may enable assessment of fracture healing. The aim of this study was to establish a reliable method for three-dimensional reconstruction of sonographic callus. Patients that underwent non-operative management of displaced midshaft clavicle fractures and intramedullary nailing of tibia fractures were prospectively recruited and followed to union. Ultrasound scanning was performed at periodical time points following injury. Infra-red tracking technology was used to map each image to a three-dimensional lattice. Criteria was fist established for two-dimensional bridging callus detection in a pilot study. Using echo intensity of the ultrasound image, semi-automated mapping was used to create an anatomic three-dimensional representation of fracture healing. Agreement on the presence of sonographic bridging callus was assessed using the kappa coefficient and intra-class-correlation (ICC) between observers. 112 clavicle fractures and 10 tibia fractures completed follow-up at six months. Sonographic bridging callus was detected in 62.5% (n=70/112) of the clavicles at six weeks post-injury. If present, union occurred in 98.6% of the fractures (n=69/70). If absent, nonunion developed in 40.5% of cases (n=17/42)(73.4%-sensitive and 100%-specific to predict union). Out of 10 tibia fractures, 7 had bridging callus of at least one cortex at 6 weeks and when present all united. Of the three patients lacking sonographic bridging callus, one went onto a nonunion (77.8%-sensitive and 100%-specific to predict union). The ICC for sonographic callus between four reviewers was 0.82 (95% CI 0.68–0.91). Three-dimensional ultrasound reconstruction of bridging callus has the potential to identify impaired fracture healing at an early stage in fracture management

Abstract. Objectives. Three-dimensional visualisation of sonographic callus has the potential to improve the accuracy and accessibility of ultrasound evaluation of fracture healing. The aim of this study was to establish a reliable method for producing three-dimensional reconstruction of sonographic callus. Methods. A prospective cohort of ten patients with a closed tibial shaft fracture managed with intramedullary nailing were recruited and underwent ultrasound scanning at 2-, 6- and 12-weeks post-surgery. Ultrasound B-mode capture was performed using infrared tracking technology to map each image to a three-dimensional lattice. Using echo intensity, semi-automated mapping was performed by two independent reviewers to produce an anatomic three-dimensional representation of the fracture. Agreement on the presence of sonographic bridging callus on three-dimensional reconstructions was assessed using the kappa coefficient. Results. Nine of the ten patients achieved union at six months. At six weeks, seven patients had bridging callus at ≥1 cortex on the three-dimensional reconstruction; when present all united. Compared to radiographs, no bridging callus was present in any patient. Of the three patients lacking sonographic bridging callus, one went onto a nonunion (77.8%-sensitive and 100%-specific to predict union). At twelve weeks, nine patients had bridging callus at ≥1 cortex on three-dimensional reconstruction and all united (100%-sensitive and 100%-specific to predict union). Compared to radiographs, seven of the nine patients that united had bridging callus. Three-dimensional reconstruction of the anteromedial and anterolateral tibial surface was achieved in all patients, and detection of sonographic bridging callus on the three-dimensional reconstruction demonstrated substantial inter-observer agreement (kappa=0.78, 95% confidence interval 0.29–1.0, p=0.011). Conclusions. Three-dimensional fracture reconstruction can be created using multiple ultrasound images in order to evaluate the presence of bridging callus. This imaging modality has the potential to identify impaired healing at an early stage in fracture management. Declaration of Interest. (b) declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research reported:I declare that there is no conflict of interest that could be perceived as prejudicing the impartiality of the research project

Introduction: Cadaveric intervertebral discs (IVD) must perform consistently and repeatably with time and cyclic loading if the results from long experimental protocols are to be considered valid. Experiment design should take into account the potential for changes in the biomechanical properties of the intervertebral disc. Changes in the pressure distribution and stress profiles across the IVD along with variation in movement of the anterior annulus during a load cycle give a good indication as to the biomechanic status of the IVD. The purpose of this study was to assess the biomechanic response of the IVD to repeated cyclic loading, in normal, flexed and extended positions over a prolonged period. Methods: Ten multisegment cadaveric lumbar spine specimens (L3-5 or L1-3) were dissected and compressed to 1kN in 6° flexion, neutral and 4° extension. The anterior annulus was imaged during loading using ultrasound. The stress distribution along the mid-sagittal and antero-postero-lateral (APL) diameters of both discs was measured by withdrawing a miniature pressure transducer from posterior to anterior across the IVD during loading. Stress profilometry and ultrasound imaging was performed over a two day period. Results: Ultrasound imaging provides an easy method for observing disc movement during compressive loading of a multi-segment specimen through positions of extension and flexion. Anterior disc bulging increased by more than 150% as the specimen is loaded from 4° of extension to 6° flexion. Repeated passes of the pressure transducer across both the mid-sagittal and APL diameter of the discs produced repeatable stress profiles. Similarly, ultrasound imaging of the anterior annulus showed comparable disc movement after cyclic loading. Conclusions: Preliminary results suggest that the biomechanical behaviour of the IVDs of a multi-segment specimen do not change significantly following prolonged testing and multiple cyclic loading

Shortening of patellar tendon after total knee arthroplasty (TKA) was previously reported by several studies. Its etiology still remains controversial. Patellar tendon shortening, a direct cause of patella baja, has a dramatic negative impact in terms of clinical outcomes after TKA. Main objective of this study is to assess the feasibility of utilizing a different technique with Ultrasound that is easy to use, cost-effective and able to eliminate the problem of differential magnification occurring in other techniques which count on standard x-rays and to establish the correlation between clinical outcomes and changes in patellar tendon length and thickness after TKA. The study was designed as prospective cohort and, after a minimum of 4-year-follow up period, 47 knees of 24 patients who had undergone primary TKA without patellar resurfacing were included in the study. All patients were scored with Kujala and HSS scores and all patellar tendons were evaluated with USG regarding their length and thickness. We used conventional grey-scale ultrasound imaging (US) to determine any changes in patellar tendon morphology. All cases were evaluated by the same radiologist. The patellar tendon was examined with the knee in 30° flexion. The flexion angle helped to stretch the extensor mechanism and avoid anisotropy (concavity) of the patellar tendon. The transducer was placed along the long axis of the tendon. The patellar tendon was initially examined in the longitudinal plane in order to measure the total length. Then, total length was divided into three parts and sagittal thickness was calculated at the proximal, median, and distal thirds of the patellar tendon. Both the length and thickness of the tendon were measured before surgery and at the 4th year of follow-up. Of the 47 knees that were included in our study, the mean pre-operative and postoperative length of the patellar tendon was 40.78±6.15 mm and 35.93±4.52 mm. Our results suggested significant shortening of the patellar tendon after primary TKA surgery (p<0.05). Intergroup analysis suggested that reduced sagittal thickness in the proximal third of the tendon was more strongly correlated with an increase in functional outcomes (p<0.05). Our results suggested no significant difference in clinical outcome scores between patients with increased or decreased length of the patellar tendon after TKA (p>0.05). We suggest that determining morphologic changes in sagittal thickness as well as length is important in explaining some of the ambiguous causes of anterior knee pain and impaired clinical outcomes after TKA. More accurate documentation of morphologic changes in the patellar tendon after TKA will certainly help to develop new techniques by surgeons or avoid some existing routines that may harm the tendon. USG is a feasible method for evaluating patellar tendon morphology after TKA but more future studies are needed

Objective . Dunkin Hartley guinea pigs, a commonly used animal model of osteoarthritis, were used to determine if high frequency ultrasound can ensure intra-articular injections are accurately positioned in the knee joint. Methods. A high-resolution small animal ultrasound system with a 40 MHz transducer was used for image-guided injections. A total of 36 guinea pigs were anaesthetised with isoflurane and placed on a heated stage. Sterile needles were inserted directly into the knee joint medially, while the transducer was placed on the lateral surface, allowing the femur, tibia and fat pad to be visualised in the images. B-mode cine loops were acquired during 100 µl. We assessed our ability to visualise 1) important anatomical landmarks, 2) the needle and 3) anatomical changes due to the injection. . Results. From the ultrasound images, we were able to visualise clearly the movement of anatomical landmarks in 75% of the injections. The majority of these showed separation of the fat pad (67.1%), suggesting the injections were correctly delivered in the joint space. We also observed dorsal joint expansion (23%) and patellar tendon movement (10%) in a smaller subset of injections. Conclusion. The results demonstrate that this image-guided technique can be used to visualise the location of an intra-articular injection in the joints of guinea pigs. Future studies using an ultrasound-guided approach could help improve the injection accuracy in a variety of anatomical locations and animal models, in the hope of developing anti-arthritic therapies. Cite this article: Bone Joint Res 2015;4:1–5

Introduction. Knee arthroplasty is an effective intervention for painful arthritis when conservative measures have failed. Despite recent advances in component design and implantation techniques, a significant proportion of patients experience problems relating to the patella-femoral joint (PFJ). Detailed knowledge of the shape and orientation of the normal and replaced femoral trochlea groove is critical when considering potential causes of anterior knee pain. Furthermore, to date it has proved difficult to establish a diagnosis due to shortcomings in current imaging techniques for obtaining satisfactory coronal plane motion data of the patella in the replaced knee. The aim of this study was to correlate the trochlea shape of normal and replaced knees with corresponding coronal plane PFJ kinematic data. Method. Bony and cartilagenous trochlea geometries from 3T MRI scans of 20 normal healthy volunteers were compared with both anatomical and standard total knee replacements (TKR) and patellofemoral joint replacement (PFJR) geometries. Following segmentation and standardized alignment, the path of the apex of the trochlea groove was measured using customized Matlab software. (Fig1). Next, kinematic data of the 20 normal healthy volunteers (Normal) was compared with that of 20 TKR, and 20 PFJR patients using the validated MAUS. TM. system (Motion Analysis and UltraSound) comprising a 12-camera, motion capture system used to capture images of reflective markers mounted on subjects lower limbs and an ultrasound probe. A mapping between the ultrasound image and the motion capture system allows the ultrasound probe to be used to determine the locations of the patella relative to bony landmarks on the femur during a squat exercise. Results. In normal knees the arc of the trochlear groove apex was orientated progressively laterally for both cartilage and. Neither of these trends were reproduced by any of the knee prostheses. Indeed far from being a laterally directed trochlea groove, both the anatomic TKR and PFJR have a medially orientated trochlea, whilst the TKR showed a neutral straight path (Figure 2). The direction of displacement in the replaced knee is significantly different (opposite) to that of the native knee (p<0.05). The accuracy of the MAUS technique registering the ultrasound images within the motion capture system is 1.84 mm (2 × SD). The three groups showed very different patella tracking patterns which matched the orientation of the underlying trochlea (Figure 3). The sine wave pattern of coronal plane patella motion displayed by the Normal group was not recreated in the TKR or PFJR groups. Movements of the Normal group were significantly different from the TKR group (p=0.03) and the PFJR group (p<0.01), whilst there was no significant difference between the TKR and PFJR groups (p=0.27). Discussion. We present a new, accurate, reliable in vivo technique for measuring 3D patellofemoral kinematics in native and replaced knees. Our data suggest that many aspects of patellofemoral kinematics are absent following TKR and PFJR. This can be explained by the differences in shape of the underlying femoral component. Anterior knee pain problems might be addressed by alterations to the patellofemoral joint in future designs of knee arthroplasty

Aim. To systematically review the literature and anatomical atlases on LM morphology. Methods. Relevant studies were searched in PubMed (Medline) and Science Direct. Anatomical atlases were retrieved from multiple university libraries and online. Included atlases and studies were assessed at five items: visuals present(y/n), quality of visuals(in-/sufficient), labelling of multifidus (y/n), clear description of region of interest(y/n), description of plane has been described(y/n). This risk of bias assessment tool was developed to assess the quality of description of anatomy, since existing risk of bias tables have only been developed to assess the methodology of studies. Results. In total 69 studies and 19 anatomical atlases were included. Studies. - 52 of 69 studies, LM was described as a superficial muscle at the levels L4 – S1. Others presented the LM as deep intrinsic muscle. - Most used methods: MRI, ultrasound imaging or drawings. - 32 of 69 studies scored a total of five points at the risk of bias assessment, which means low risk of bias. Anatomical atlases. - LM is shown as a deep intrinsic back muscle covered by the erector spinae and fascia thoracolumbalis. - Most anatomical atlases (8/19) had a score of four points at the risk of bias assessment. Conclusion. Anatomy atlases reported different LM morphology compared to anatomical studies. Even between studies, there appears to be inconsistent reporting in LM anatomy. Variation in research methods that are used for measuring LM morphology could influence variation in describing and presenting LM morphology. Standardization of research methodology is recommended in order to compare studies. No conflicts of interest. Sources of Funding: SIA RAAK-Publiek

In view of possible clinical applications of mesenchymal stromal cells (MSCs), interesting results in repairing the Achilles tendon have been achieved in rabbit models since 1997. Histological and immunochemical studies have demonstrated the quality of repair. A basic problem in tissue repair is the way to administer stem cells. Several questions remain:. have the cells to be differentiated or not?. Could cells be administered without using scaffolds?. Attempting to cure, as a clinical model, horses with a pathological core lesion in the superficial digital flexor tendon (SDFT), MSCs were recovered from autologous bone marrow, expanded ex vivo, suspended in autologous serum and re-injected directly into the core lesion. All 11 horses implanted with autologous MSCs exhibited no adverse reaction due to the implantation of the cells, either locally or systemically. After rehabilitation therapy nine MSC-treated animals recovered from their clinical conditions, had an excellent ultrasound image of tendons after a period ranging from 3 to 6 months, and returned to racing with good or even optimal results in the previous category of competition in 9 to 12 months without any re-injuring event. All of them are still active more than 2 years from diagnosis. One of the 2 remaining horses received less than 1×106 of MSCs, and its tendon did not heal relapsing after rehabilitation, the other was lost to follow-up. In contrast, most of horses from the control group showed tendon ultrasound images that revealed fibrosis during the healing process, and all of them were re-injured after a median time of 7 months. The ability of tissue microenvironments to induce cell differentiation could render unnecessary a partial or total ex vivo differentiation and direct infusion of undifferentiated MSCs could represent a safe therapeutic approach to tendon repair

Typical navigation system to insert hip implants in the accurate position consists of a 3D position measurement device and a computer. These navigation systems are classified into two categories according to the method of identifying the anterior pelvic plane that works as the reference of the orientation of the acetabulum cup. The preparation process for imageless navigation system is very easy because it uses three anatomical bony markers to define the anterior pelvic plane. When these anatomical bony markers are hard to locate, especially at the pubic symphysis due to the thick soft tissue, the accurate direction of the cup cannot be secured. The aim of this study is to estimate the soft tissue thickness without using the patient’s specific data such as the A-mode ultrasound image or C-arm image. In our previous study, it was pointed out that the thickness of the hypodermic fat obtained through an ultrasound image could be estimated using the patient’s BMI and the displacement created by a specific force. Considering the probe shape, the soft-tissue thickness estimation formula is expressed as follows:. \batchmode \documentclass[fleqn,10pt,legalpaper]{article} \usepackage{amssymb} \usepackage{amsfonts} \usepackage{amsmath} \pagestyle{empty} \begin{document} \[Y_{estimated_thickness}\ =\ k(b_{0}\ +\ b_{1}\ {\times}\ BMI\ +\ b_{2}{\times}\ {\delta})\] \end{document}. k: constant for the shape of the probe end. Only two kinds of the probe end shapes (flat-ended probe and spherical-ended probe) were considered, and the change in the k value corresponding to the radius was calculated using the FE model of the soft tissue for each subject. The finite-element model was constructed as axisymmetric. The simulation result of the initially assumed variables and the measured result were compared, and the optimization method was used to minimize the error: The RMS difference between the result of the experiment and that of the analysis was taken as the objective function. With the FE analysis for the two kinds of probe shapes with one subject, we determined the shape variable (k). From the formula composed by a model with data from 28 people, the average error was 3 mm equivalent to the angle error of less than 1°. Therefore, the use of the method suggested in this study will help to improve the acetabulum cup navigation in THA, when we use only the surface points on the soft tissue. In addition, it seems that the soft-tissue thickness estimation formula suggested in this study may be generally used

This study of collegiate basketball players evaluated change over time (COT) in ultrasound shear wave (SW) elastography metrics across the basketball season, and correlated to morphologic changes on conventional ultrasound imaging, and VISA-P scores. In eleven male collegiate basketball players (mean age 19, age range 18–21), patella tendon (PT) ultrasound and SW elastography of both knees were performed at pre-season and post-season time points, and players reported their VISA-P scores throughout the season. Patella tendinopathy grade and SW metrics were correlated to VISA-P scores using Spearman correlation coefficients. Paired t-test was used to assess differences in mean SW metrics at pre-and post-season timepoints, accounting for leg dominance. 6 of 11 players (54.5%) had baseline patella tendinopathy on ultrasound progressing in 4 players. The mean change in VISA-P score was 15.18 (+/−8.55). No significant correlation was seen between ultrasound grades of tendinopathy and VISA-P. Pre-season SW velocities did not significantly correlate with baseline VISA-P scores. Post-season SW values and SW COT demonstrated strong correlation with change in VISA-P score in dominant and non-dominant knees. Although not statistically significant, there was a trend towards higher SW velocity for tendinopathy in both dominant and non-dominant knees at both study visits. SW metrics of the PT correlated to change in VISA-P scores in the dominant and non-dominant knees, whereas conventional ultrasound grades of patella tendinopathy did not. There was a trend towards higher SW velocities in patella tendinopathy which may indicate detection of change in intrinsic tissue stiffness

Aim: To evaluate the accuracy of intra-operative point acquisition during navigated hip replacement using an ultrasound transducer probe relative to a percutaneous digitiser stylus (pointer). To study intra- and inter-observer variability with the use of the ultra-sound transducer and percutaneous digitiser point probes. To assess the learning curve with the use of the ultrasound transducer probe. As part of a larger cadaver study evaluating navigated total hip replacement via the posterior approach, we assessed data relating to acquisition of bony landmarks of the Anterior Pelvic Plane (APP) by four surgeons with an ultrasound transducer and a percutaneous point probe. The surgeons had differing levels of experience with hip surgery in general, and also with surgical navigation per se, but none of them had previously used the ultrasound probe for the specific purpose of landmark acquisition. Without fixing an absolute positional value for any of the bony landmarks, the points registered for individual landmarks by each surgeon were then studied, looking at the three-dimensional spread of these points relative to each other about the mean value. The data from all four surgeons were analysed, looking at the global dispersion of points acquired by the ultrasound and percutaneous point digitiser probes. Our results show that with the exception of a few isolated outliers, the ultrasound probe generated values fell within a +/− 10 mm range. For all four surgeons, the global spread of ultrasound-registered points was noted to be less than that acquired by percutaneous point probe acquisition. Of interest was the finding that points registered by individual surgeons using the ultrasound probe tended to be grouped distinctly together but spatially separate from those of the other surgeons; it would appear that each operator was “homing” in on what he perceived to be the bony landmark in question on the projected ultrasound image. With the percutaneous pointer probe, and with the anterior superior iliac spines as the target, there was closer grouping of points around the mean positional value for the two surgeons who were experienced with its use. However, at the symphysis pubis, the spread of points for these surgeons were not much different from the other two less experienced one, with these points showing a global spread as great as 25 mm. Regardless of the experience of the surgeon, the use of the ultrasound transducer probe appears to be more accurate than percutaneous pointer probe for acquisition of the bony landmarks that constitute the anterior pelvic plane. The learning curve associated with its use is seemingly short and steep. Its accuracy is limited by the fact that the identification of the bony land marks on the on-screen display is open to interpretation by the individual. Methods to standardise the identification of these landmarks on ultrasound images may help improve its accuracy in the future