Objectives

The objective of this study was to compare the elution characteristics, antimicrobial activity and mechanical properties of antibiotic-loaded bone cement (ALBC) loaded with powdered antibiotic, powdered antibiotic with inert filler (xylitol), or liquid antibiotic, particularly focusing on vancomycin and amphotericin B.

Objectives. Irrigation is the cornerstone of treating skeletal infection by eliminating pathogens in wounds. A previous study shows that irrigation with normal saline (0.9%) and ethylenediaminetetraacetic acid (EDTA) could improve the removal of Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) compared with normal saline (NS) alone. However, it is still unclear whether EDTA solution is effective against infection with drug-resistant bacteria. Methods. We established three wound infection models (skin defect, bone-exposed, implant-exposed) by inoculating the wounds with a variety of representative drug-resistant bacteria including methicillin-resistant S. aureus (MRSA), extended spectrum beta-lactamase-producing E. coli (ESBL-EC), multidrug-resistant Pseudomonas aeruginosa (MRPA), vancomycin-resistant Enterococcus (VRE), multidrug-resistant Acinetobacter baumannii (MRAB), multidrug-resistant Enterobacter (MRE), and multidrug-resistant Proteus mirabilis (MRPM). Irrigation and debridement were repeated until the wound culture became negative. The operating times required to eliminate pathogens in wounds were compared through survival analysis. Results. Compared with other groups (NS, castile soap, benzalkonium chloride, and bacitracin), the EDTA group required fewer debridement and irrigation operations to achieve pathogen eradication in all three models of wound infection. Conclusion. Irrigation with EDTA solution was more effective than the other irrigation fluids used in the treatment of wound infections caused by drug-resistant pathogens. Cite this article: Z. Deng, F. Liu, C. Li. Therapeutic effect of ethylenediaminetetraacetic acid irrigation solution against wound infection with drug-resistant bacteria in a rat model: an animal study. Bone Joint Res 2019;8:189–198. DOI: 10.1302/2046-3758.85.BJR-2018-0280.R3

Athletes are prone to iterated traumatic straining of sacroiliac joints and insertion of muscles in pelvic bones. The aim of our study was to show the connection between iterated trauma and staphylococcal infection in athletes. A compilation of data concerning 22 patients, all high-ranking athletes in Sweden, with pelvic skeletal infection (symphysitis, sacroiliitis and acetabular necrosis) is presented. Most of them were ball-players, 2 females and 20 males with an average age of 21 years. The onset was acute or subacute and the disease was commonly misinterpreted, mainly as an acute abdominal disease. Four patients erroneously underwent laparotomy for suspected appendicitis. Eight patients had skin lesions (5 purulent and 3 uninfected) at the onset and thus had a portal for possible entry for a bacterial impact in the pelvic skeleton. The only verified bacterial aetiology was Staphylococcus aureus in 19 cases (culture of blood and/or aspirated samples in 18 and serology only in 1). An early scintigraphy was of greater value than X-ray for diagnosis and was positive in 8/10 evaluable cases. In all, 12/18 had early positive plain radiological findings. Another patient (#22) had, except skeletal infection, a psoas abscess, diagnosed by Magnetic Resonance Imaging (MRI). Antistaphylococcal drugs administered parenterally for 1–2 weeks followed by oral drugs for a few months comprised a successful treatment and no patient had serious sequelae or relapse. The occurrence of abdominal and/or lower back pains concomitant to fever in young persons engaged in elite team sports should lead to a suspicion of pelvic pyogenic infection. Early scintigraphy and cultures of blood and aspirated material from the infection site was essential for the diagnosis. MRI has also been shown to be valuable

Aims

Treatment outcomes for methicillin-resistant Staphylococcus aureus (MRSA) periprosthetic joint infection (PJI) using systemic vancomycin and antibacterial cement spacers during two-stage revision arthroplasty remain unsatisfactory. This study explored the efficacy and safety of intra-articular vancomycin injections for PJI control after debridement and cement spacer implantation in a rat model.

Skeletal Cryptococcosis although rare has been reported in immunodeficient individuals and in particular those with HIV. We present a case in a HIV- negative patient who presented to the London Sarcoma service masquerading as a primary bone tumour and review the relevant literature. A 71 year old lady presented with a three month history of right submammary pain associated with a new lump. Chest radiographs showed an osteolytic lesion in the right 6. th. rib. CT scans demonstrated mediastinal lymphadenopathy and numerous lung nodules. Differential diagnosis of the lesion included TB abscess, myeloma, lymphoma or as a primary lung tumour presenting with hilar lymphadenopathy and necrotic skeletal metastasis. CT guided biopsy was performed with histology showing necrotising granulomatous inflammation with numerous yeast like organisms in keeping with Cryptococcus fungal infection. She was treated successfully with a six week course of voriconazole. Cryptococcal skeletal infections can cause significant morbidity and mortality and should be considered as a rare cause of lytic osseous lesions

Purpose of study: Septic arthritis and osteomyelitis have traditionally been managed by intravenous antibiotics for 3 to 6 weeks. This requires a prolonged in patient stay, inconvenience to parents, morbidity and cost. A number of authors have suggested that a shortened course of intravenous antibiotics for 7–10 days may be as effective. This studies reviews the outcomes of a short course regime started in 2001. Methods: We prospectively reviewed 34 cases of acute osteomyelitis and 28 cases of acute septic arthritis in children. These were confirmed by a positive blood culture or a positive aspirate culture or raised WCC in joint aspirate for septic arthritis; or a positive bone scan/culture for osteomyelitis. Patients were treated with a 3 day course of intravenous antibiotics, following surgical drainage of joints when required. Providing the clinical and biochemical parameters were improving patients then received 3 weeks oral antibiotics. The duration of IV administration and of inpatient stay and any incidence of readmission/reoccurrence was noted. Serial measurements of inflammatory markers were recorded. Results: 35 of the 62 patients received intravenous antibiotics for < 4 days. Mean in-patient stay was 5.5 days. There was one re-admission for recurrence of infection. One patient required a repeat joint washout at 7 days. At 3 months no patients had ongoing infection. There was a correlation between CRP levels and the severity of infection, and therefore the length of treatment required. Conclusions: We suggest acute suppurative skeletal infection can be managed safely with a shortened course of intravenous and oral antibiotics (following surgical drainage in the case of intra articular infection). Patients must be observed closely by experienced practitioners

Aim: Septic Arthrits & osteomylelitis has traditionally been managed by intravenous antibiotics for 4 to 6 weeks. This requires a prolonged in patient stay, inconvience to parents, morbidity and unnecessary cost. A number of authors have suggested that shortened course of intravenous antibiotics 7–10 days are effective. Methods: In 2001 we started to prospectively evaluate a shortened 3 day of intravenous antibiotic regime. We prospectively treated 36 cases of acute osteomyelitis and 30 cases of acute septic arthritis in children. These were confirmed by positive blood culture, positive aspirate culture, raised WCC in joint aspirate for septic arthritis or positive bone scan/culture for osteomyelitis. These patients were treated with a shortened course (3 days) of intravenous antibiotics following surgical drainage when required. Serial measurements of inflammatory markers and clinical status were recorded. On Day 4 of admission if clinical and biochemical parameters improved patients commenced high dose oral antibiotics. If no improvement they continued IV abx and consideration for repeat washout given. Patients discharged with three week course of antibiotics. Endpoint analysis of duration of IV administration, inpatient stay, readmission/reoccurrence was undertaken. Results: 43 of the 66 (66%) patients received were discharged by Day 5 after receiving 3 full days of intravenous antibiotics. Mean in-pt stay was 5.5 days. There was one readmission for intolerance of high dose antibiotics. 6 septic patients required a repeat washout (Day 4–7 of admission). At 3 months there were no patients with ongoing infection. Conclusion: We suggest the vast majority of acute suppurative skeletal infection can be managed safely with shortened course of intravenous and oral antibiotics following surgical drainage (in the case of intra articular infection). About 25% of patients will need longer courses of antibiotics and possibly repeat washout. This subgroup can be identified by careful clinical evaluation and measurement of inflammatory markers

Flaps constitute an integral part of the treatment of soft tissue and skeletal infections of the extremities, focusing on the coverage and augmentation of the local biology. In a 6-year period, a total of 33 septic defects of the upper (6) and lower (27) extremities were treated with 4 free and 29 pedicled flaps, after extensive surgical debridement of the septic site. In the lower extremity, treatment included 3 free (2 latissimus dorsi and 1 serratus anterior), and 24 pedicled flaps (5 heads of gastrocnemius, 7 soleus, 1 abductor hallucis, 9 reverse fasciocutaneous, 1 combined medial head of gastrocnemius and soleus and 1 extensor longus hallucis) for 3 cases of soft tissue sepsis and 24 septic defects of the skeleton. In the upper extremity, 1 free vascularised fibular graft (combined with muscle-skin) and 5 pedicled flaps (2 homodigital, 1 heterodigital, 1 cross-finger, 1 periosteal) were used for 3 soft tissue and 3 skeletal septic defects. All but one flaps of the lower extremities were covered with split thickness skin (simultaneously or within 7 days), whereas flaps of the upper extremity included skin in all cases. Three flaps (2 reverse fasciocutaneous and one soleus) were revised (with latissimus dorsi, serratus anterior and extensor longus hallucis flaps respectively) in a mean period of 4 months due to persistent infection and 4 skin grafts were revised due to superficial infection. In a minimum follow-up period of 9 months (9–60 months) full coverage of the defect and treatment of infection was accomplished in all patients, resulting in a good functional and aesthetic outcome. Except for 2 patients, all were able to walk and use their extremity and returned to previous activities. The use of flaps in the treatment of septic skeletal or soft tissue defects leads to a functional upper or lower extremity and successfully prevents amputation

Spinal epidural sepsis is more widely recognised with MRI. Treatment includes antibiotics, multisystem support and drainage of pus. Neurological loss will often be stabilised but dramatic recovery is infrequent, explaining the importance of early intervention. This series highlights a very sinister spectrum of spinal infective disease despite ideal traditional treatment for spinal skeletal infection. This is a retrospective case series review of five patients. All patients presented with regional spinal pain, fever and regional musculoskeletal infective foci (e.g. discitis). Mild neurological abnormality existed in three patients. Rapid multisystem collapse occurred with the need for ventilatory support, despite institution of appropriate antibiotic treatment for all patients. All had grown Staphylococcus Aureus from blood cultures. Subsequent extensive quadriparesis/plegia developed, and repeat imaging demonstrated wide spread epidural pus in the cervical spine. Surgical treatment was considered but not performed when the prognosis was so poor neurologically and medically, and when the widespread epidural pus was so inaccessible. All patients died rapidly upon withdrawal of supportive treatment. This paper describes a sinister spectrum of spinal infection with catastrophic complications despite “appropriate” treatment for previously diagnosed spinal foci infection. Positive blood cultures and fever alert to these dangers, and multisystem collapse heralded the development of cervical epidural infection. Possible interventions include early MRI scanning of the whole spine, more aggressive (than traditionally accepted) surgical treatment of infective foci in the spine in these circumstances, and minimally invasive cervical canal decompression procedures with multiple laminotomies

Septic Arthrits & osteomylelitis has traditionally been managed by intravenous antibiotics for 4 to 6 weeks. This requires a prolonged in patient stay, inconvenience to parents, morbidity and unnecessary cost. A number of authors have suggested that shortened course of intravenous antibiotics 7–10 days are effective. In 2001 we started to prospectively evaluate a shortened 3 day of intravenous antibiotic regime. We prospectively treated 36 cases of acute osteomyelitis and 30 cases of acute septic arthritis in children. These were confirmed by positive blood culture, positive aspirate culture, raised WCC in joint aspirate for septic arthritis or positive bone scan/culture for osteomyelitis. These patients were treated with a shortened course (3 days) of intravenous antibiotics following surgical drainage when required. Serial measurements of inflammatory markers and clinical status were recorded. On Day 4 of admission if clinical and biochemical parameters improved patients commenced high dose oral antibiotics. If no improvement they continued IV abx and consideration for repeat washout given. Patients discharged with three week course of antibiotics. Endpoint analysis of duration of IV administration, inpatient stay, readmission/ reoccurrence was undertaken. Results: 43 of the 66 (66%) patients received were discharged by Day 5 after receiving 3 full days of intravenous antibiotics. Mean in-pt stay was 5.5 days. There was one re-admission for intolerance of high dose antibiotics. 6 septic patients required a repeat washout (Day 4–7 of admission). At 3 months there were no patients with ongoing infection. Conclusion: We suggest the vast majority of acute suppurative skeletal infection can be managed safely with shortened course of intravenous and oral antibiotics following surgical drainage (in the case of intra articular infection). About 25% of patients will need longer courses of antibiotics and possibly repeat washout. This subgroup can be identified by careful clinical evaluation and measure

Introduction: Osteomyelitis and septic arthritis (SA) below the elbow are severe conditions affecting the function and viability of the hand. Factors predisposing to these conditions and parameters affecting prognosis are emphasized. Material and Methods: In a 4 years period, 16 patients with SA (4) or osteomyelitis (12) were treated in a University Orthopaedic Department providing care to a rural population of 1.000.000, Nine had history of trauma in unhygienic environment, 3 had immunodeficiency and in 4 osteomyelitis was iatrogenic [previous fixation of fractures (3) and vein catheterization (1)]. SA was located in the wrist (2) and thumb joints (2) and osteomyelitis involved the radius (3), ulna (1), metacarpals (3) and phalanges (5). Ten patients received oral antibiotics in other centers. The mean delay from onset of symptoms to referral to our center was 22 days. Apart of antibiotics administration, surgical treatment included debridement and irrigation for SA and excision of necrotic bone, stabilization (external fixators), use of antibiotic beads and secondary bridging with bone graft for osteomyelitis. Additional procedures (amputations, arthrodesis) were also required in some cases. Results: Cultures were positive in 9 of 16 cases [Staph. aureus (5) and enterobacter cloacae (3)]. Patients underwent multiple procedures (mean: 3.8) and 4 underwent amputation of a digit (2) and hand (2) due to the rapid extension of infection threatening and finally taking the lives of 2 elderly and immunosuppressed patients. Mean follow-up period of the 12 surviving and non-amputated patients was 18 months. Union was accomplished in all cases. Functional results were excellent or very good in 10 of 12 patients and good in 2 patients. All patients were satisfied and returned to their previous occupations. Conclusions: Osteomyelitis and SA below the elbow was frequent in population living in unhygienic environment or working with soil. All cases received medical treatment with delay. Immunosuppressing conditions favored the extension of infection and threatened patients lives. Delay in treatment in combination to immunosuppression resulted to significant morbidity. Early treatment including surgical drainage, thorough debridement and antibiotic administration is necessary for elimination of skeletal infection and salvage of the hand and patients life

Antibiotic resistance represents a threat to human health. It has been suggested that by 2050, antibiotic-resistant infections could cause ten million deaths each year. In orthopaedics, many patients undergoing surgery suffer from complications resulting from implant-associated infection. In these circumstances secondary surgery is usually required and chronic and/or relapsing disease may ensue. The development of effective treatments for antibiotic-resistant infections is needed. Recent evidence shows that bacteriophage (phages; viruses that infect bacteria) therapy may represent a viable and successful solution. In this review, a brief description of bone and joint infection and the nature of bacteriophages is presented, as well as a summary of our current knowledge on the use of bacteriophages in the treatment of bacterial infections. We present contemporary published in vitro and in vivo data as well as data from clinical trials, as they relate to bone and joint infections. We discuss the potential use of bacteriophage therapy in orthopaedic infections. This area of research is beginning to reveal successful results, but mostly in nonorthopaedic fields. We believe that bacteriophage therapy has potential therapeutic value for implant-associated infections in orthopaedics.

Cite this article: Bone Joint J 2021;103-B(2):234–244.

Objectives

The objective of this study is to determine an optimal antibiotic-loaded bone cement (ALBC) for infection prophylaxis in total joint arthroplasty (TJA).