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Orthopaedic Proceedings
Vol. 104-B, Issue SUPP_10 | Pages 53 - 53
1 Oct 2022
Cardona CG Omiste I Johnson MCB Veloso M Gómez L Cisneros BE Camarena JHN García DB Font-Vizcarra L
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Aim

Acute post-surgical infection is one of the most serious complications after instrumented thoracolumbar fusion with an incidence of 0.7%-12%. Acute infection can lead to an increase in morbidity, mortality, and economic costs for the healthcare system.

The main objective of our study was to determine the variables associated with a higher risk of acute infection after thoracolumbar instrumentation in our center.

Methods

We conducted an observational case-control study including instrumented fusions of the thoracolumbar spine performed between 2015 and 2021 at our institution. We included patients with thoracolumbar fusions after a fracture or for the treatment of degenerative pathology.

We analyzed demographic variables related to the surgical procedure, the causative microorganism of infection, the outcome of infection treatment, and complications.

We performed a descriptive analysis of all variables and a univariate comparison of cases and controls. The dichotomous variables were compared using the Fisher test, while the quantitative variables were compared using the Student's T-test. A p-value of <0.05 is taken into account to consider the statistical significance. SPSS v25 Windows program was used for statistical analyses.


Orthopaedic Proceedings
Vol. 101-B, Issue SUPP_14 | Pages 22 - 22
1 Dec 2019
Veloso M Bernaus M Angles F Gómez L Cubillos YL Soto S Font-Vizcarra L
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Aim

To evaluate bacterial adhesion and biofilm formation to metallic cerclage wire versus polymer cerclage system (SuperCable®)

Methods

Experimental in vitro study to evaluate quantitative bacterial adherence to different cerclage wire materials. Two types of cerclage wires were compared: a metallic versus a polymer based wire (SuperCable®).

A two-centimeter cerclage wire piece of each material was included in 2 mL of tryptic soy broth (TSB) culture media, inoculated with 10 microliters of a 0.5 McFarland of a Staphylococcus epidermidis strain and cultivated at 37°C during 2h for adhesion and 48h for biofilm formation. After this time, the cerclages were washed using a 1% phosphate buffered saline (PBS) and sonicated in new culture medium. After sonication, dilutions of each culture were spread in TSB agar and incubated 37°C during 24h. The number of colonies were counted and the cfu/cm2 was calculated.


Orthopaedic Proceedings
Vol. 100-B, Issue SUPP_17 | Pages 73 - 73
1 Dec 2018
Muñoz-Gamito G Cuchí E Roige J Matamala A Gómez L Haro D Pérez J
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Aim

To determine whether rep-PCR genotyping can improve the diagnosis of coagulase-negative staphylococci(CoNS)bone and joint infection relative to the standard method based on phenotypic identification.

Method

Observational study comparing diagnostic tests (January 2011-March 2015), including all orthopaedic surgery patients with clinically suspected infection and ≥2 surgical specimens culture-positive for CoNS. Data collection included epidemiologic and clinical information, current clinical signs of suspected infection, and microbiological information. Each CoNS strain was analyzed by both methods (phenotyping, VITEK and API;and genotyping, rep-PCR). In accordance with current IDSA guidelines, CoNS strains identified as identical in ≥2 samples within the same surgical episode were considered pathogenic. The results of the two techniques were compared and statistically analyzed.


Orthopaedic Proceedings
Vol. 100-B, Issue SUPP_17 | Pages 50 - 50
1 Dec 2018
Huguet S Gómez L Bernaus M Matamala A Cuchi E Font-Vizcarra L
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Aim

In two-stage replacements for septic loosening, some studies have suggested that associate bacterial colonization of spacers had a worse result in relation to the control of the infection and a higher rate of complications after the implantation of the definitive prosthesis.

The aim of our study was to determine the reoperation rate of patients undergoing two-stage revision surgery according to the results of spacer sonication.

Method

A retrospective observational study was conducted in which 56 hip or knee spacers implanted at our center from 2010 to 2017 were analysed. Patients were grouped into three categories:

Patients with positive spacer sonication fluid culture, with or without positive cultures from the rest of the samples.

Patients with negative spacer sonication culture and negative second-stage intraoperative cultures.

Patients with negative spacer sonication culture but positive cultures of the rest of intraoperative samples.