CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL

WS Khan; S Dheerendra; DS Johnson; JG Andrew; TE Hardingham

doi:10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Current issue

Knee

CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL

British Association for Surgery of the Knee (BASK)

WS Khan
S Dheerendra
DS Johnson
JG Andrew
TE Hardingham

CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL

Khan W, Dheerendra S, Johnson D, Andrew J, Hardingham T. CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL. Orthop Procs. 2012;94-B(SUPP_IX):82-82. doi:10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Khan, WS, et al. “CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL.” Orthopaedic Proceedings, vol. 94-B, no. SUPP_IX, 2012, pp. 82-82., https://doi.org/10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Khan, W., Dheerendra, S., Johnson, D., Andrew, J., & Hardingham, T. (2012). CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL. Orthopaedic Proceedings, 94-B(SUPP_IX), 82-82. https://doi.org/10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Khan, W., Dheerendra, S., Johnson, D., Andrew, J. and Hardingham, T. (2012) “CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL.” Orthopaedic Proceedings, 94-B(SUPP_IX), pp. 82-82. Available at: https://doi.org/10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Khan, WS, S Dheerendra, DS Johnson, JG Andrew, and TE Hardingham. “CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL.” Orthopaedic Proceedings 94-B, no. SUPP_IX (2012): 82-82. https://doi.org/10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Khan W, Dheerendra S, Johnson D, Andrew J, Hardingham T. CLONAL MESENCHYMAL STEM CELL POPULATIONS DERIVED FROM THE SYNOVIAL FAT PAD EXHIBIT A SIMILAR CELL SURFACE CHARECTERISTAION PROFILE BUT VARIABLE CHONDROGENIC DIFFERENTIATION POTENTIAL. Orthop Procs. 2012 Mar 1;94-B(SUPP_IX):82-82. https://doi.org/10.1302/1358-992X.94BSUPP_IX.BASK2010-082

Copy to clipboard

Mendeley

BibTeX

EndNote

RIS

Abstract

Introduction

Mesenchymal stem cells are a potential source of cells for the repair of articular cartilage defects. We have previously demonstrated that the infrapatellar synovial fat pad is a rich source of mesenchymal stem cells and these cells are able to undergo chondrogenic differentiation. Although synovial fat pad derived mesenchymal stem cells may represent a heterogenous population, clonal populations derived from the synovial fat pad have not previously been studied.

Materials and Methods

Mesenchymal stem cells were isolated from the infrapatellar synovial fat pad of a patient undergoing total knee arthroplasty and expanded in culture. Six clonal populations were also isolated before initial plating using limiting dilution and expanded. The cells from the mixed parent population and the derived clonal populations were characterised for stem cell surface epitopes, and then cultured as cell aggregates in chondrogenic medium for 14 days. Gene expression analyses; glycosoaminoglycan and DNA assays; and immunohistochemical staining were determined to assess chondrogenic responses.

Results

Cells from the mixed parent population and the derived clonal populations stained strongly for markers of adult mesenchymal stem cells including CD44, CD90 and CD105, and they were negative for the haematopoietic marker CD34 and for the neural and myogenic marker CD56. Interestingly, a variable number of cells were also positive for the pericyte marker 3G5 both in the mixed parent and clonal populations. The clonal populations exhibited a variable chondrogenic response; one clonal cell population exhibited a significantly greater chondrogenic response when compared with the mixed parent population.

Discussion

Pericytes are a candidate stem cell in many tissue and our results show that all six clonal populations derived from the heterogenous synovial fat pad population express the pericyte marker 3G5. The variable chondrogenic responses suggest inherent differences between these populations. The chondrogenic potential of the synovial fat pad could be optimised by the identification of clonal populations with a propensity to differentiate