Abstract
The osseointegration of implants is related to the early interactions between osteoblastic cells and titanium surfaces. The behavior of osteoblast cells was compared on four different titanium surfaces in vitro and in vivo: machined, blasted, plasma spray and micro-arc oxidation.
X-ray diffraction and scanning electron microscope investigations were performed in order to assess the structure and morphology. Biologic and morphologic responses to the osteoblast cell lines (Saos-2) were then examined, using Promega proliferation assay, alkaline phosphatase activity, vβ3 integrin expression and cytoskeleton staining (Rhodamine-Phallodine). The analysis of gene expression for osteocalcin and collagen I was done through RT-PCR. In addition, differential histologic evaluation and interfacial strength at the bone-implant interfaces were then evaluated in the distal femur of four beagle dogs.
In conclusion, micro-arc oxidation of titanium appears to exhibit more favorable osteoblast adhesion and stronger interfacial strength than the compared groups in vitro and in vivo as well.
Correspondence should be addressed to ISTA Secretariat, PO Box 6564, Auburn, CA 95604, USA. Tel: 1-916-454-9884, Fax: 1-916-454-9882, Email: ista@pacbell.net
