PAPER 069: OXIDATIVE STRESS MARKERS IN THE BLOOD OF PATIENTS WITH METAL-ON-METAL TOTAL HIP ARTHROPLASTY

Abstract

Purpose: Several studies have shown elevated levels of metal ions in the blood of patients with metal-on-metal (MM) total hip arthroplasty (THA). Even though there is no conclusive evidence that the elevated levels of ions have any detrimental effects on the patients, the presence of these ions is still a cause of concerns. The potential of metal ions released from MM implants for oxidative stress is unknown. In the present study, we measured the concentrations of oxidative stress markers in the plasma of patients with MM THAs.

Method: Blood from patients having MM THAs was collected up to 10 years post-operatively into Sarstedt Li-Heparin tubes. Plasma was prepared by centrifugation at 500 × g for 10 min. Plasma was chosen as opposed to whole blood because it is known that the assays for oxidative stress are not recommended for blood and can lead to erroneous data. Total antioxidant levels were measured by the Oxford Biomedical total antioxidant power assay to obtain an overview of the defense capacity of patients against oxidative stress. The activity of catalase and glutathione peroxidase, two antioxidant enzymes acting on specific reactive oxygen species, was measured by enzymatic assays. Peroxide concentrations were measured by the Biomedica Oxy-Stat assay to quantify damage to lipids in the systemic circulation. Nitrototyrosine levels were quantified using the BIOXYTECH® Nitrotyrosine-EIA assay to measure damage to proteins. Levels in patients without prostheses were used as control.

Results: There were no statistical differences in the concentrations of total antioxidants, lipid peroxides, and nitrotyrosines throughout the period of study. The activity of catalase and glutathione peroxidase was also stable over time. Moreover, there was no correlation between the concentrations of these markers and the concentrations of both cobalt and chromium ions.

Conclusion: Metal ions have the potential to induce the production of reactive oxygen species (free radicals) and cause oxidative stress in the plasma of patients with MM THAs. The present study showed that there were no changes in the levels of oxidative stress markers or antioxidant enzymes in these patients up to 10 years post-operatively. Taken together, the data strongly suggest that metal ions may not cause a significant oxidative stress in patients with MM THAs.