Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament

S. Y. Lee; M. Miwa; Y. Sakai; R. Kuroda; T. Niikura; M. Kurosaka

doi:10.1302/0301-620X.88B1.16795

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Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament

S. Y. Lee
M. Miwa
Y. Sakai
R. Kuroda
T. Niikura
M. Kurosaka

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Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament

Lee SY, Miwa M, Sakai Y, Kuroda R, Niikura T, Kurosaka M. Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament. J Bone Joint Surg Br. 2006;88-B(1):129-133. doi:10.1302/0301-620X.88B1.16795

Lee, S. Y., et al. “Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament.” The Journal of Bone & Joint Surgery British Volume, vol. 88-B, no. 1, 2006, pp. 129-133., https://doi.org/10.1302/0301-620X.88B1.16795

Lee, S. Y., Miwa, M., Sakai, Y., Kuroda, R., Niikura, T., & Kurosaka, M. (2006). Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament. The Journal of Bone & Joint Surgery British Volume, 88-B(1), 129-133. https://doi.org/10.1302/0301-620X.88B1.16795

Lee, S. Y., Miwa, M., Sakai, Y., Kuroda, R., Niikura, T. and Kurosaka, M. (2006) “Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament.” The Journal of Bone & Joint Surgery British Volume, 88-B(1), pp. 129-133. Available at: https://doi.org/10.1302/0301-620X.88B1.16795

Lee, S. Y., M. Miwa, Y. Sakai, R. Kuroda, T. Niikura, and M. Kurosaka. “Osteogenic potential of cells in vitro derived from haemarthrosis of the knee induced by injury to the anterior cruciate ligament.” The Journal of Bone & Joint Surgery British Volume 88-B, no. 1 (2006): 129-133. https://doi.org/10.1302/0301-620X.88B1.16795

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Abstract

We have investigated whether cells derived from haemarthrosis caused by injury to the anterior cruciate ligament could differentiate into the osteoblast lineage in vitro. Haemarthroses associated with anterior cruciate ligament injuries were aspirated and cultured. After treatment with β-glycerophosphate, ascorbic acid and dexamethasone or 1,25 (OH)₂D₃, a significant increase in the activity of alkaline phosphatase was observed. Matrix mineralisation was demonstrated after 28 days and mRNA levels in osteoblast-related genes were enhanced.

Our results suggest that the haemarthrosis induced by injury to the anterior cruciate ligament contains osteoprogenitor cells and is a potential alternative source for cell-based treatment in such injury.

Correspondence should be sent to Dr M. Miwa; e-mail: masahiko@med.kobe-u.ac.jp

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