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The Journal of Bone & Joint Surgery British Volume
Vol. 75-B, Issue 2 | Pages 334 - 335
1 Mar 1993
Nolan P Wilson D Mollan R

The Journal of Bone & Joint Surgery British Volume
Vol. 74-B, Issue 2 | Pages 284 - 286
1 Mar 1992
Nolan P Nicholas R Mulholland B Mollan R Wilson D

We cultured human osteoblasts from trabecular bone explants and confirmed their phenotype by alkaline phosphatase assay, increased cyclic adenosine monophosphate production in response to prostaglandin E2 and radiographic micro-analysis of nodules of calcification. The osteoblasts were seeded on to demineralised human bone fragments and examined at ten-day intervals over a 50-day period by scanning electron microscopy. During this time the bank bone became progressively repopulated by the cultured osteoblasts. This system may offer a means of graft enhancement in elective orthopaedic and maxillofacial surgery by delivery of cultured autologous human osteoblasts to bone defects.