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The aim of this study was to evaluate whether
coating titanium discs with selenium in the form of sodium selenite decreased
bacterial adhesion of Staphylococcus aureus and Staph.
epidermidis and impeded osteoblastic cell growth. In order to evaluate bacterial adhesion, sterile titanium discs
were coated with increasing concentrations of selenium and incubated
with bacterial solutions of Staph. aureus (ATCC
29213) and Staph. epidermidis (DSM 3269) and stained
with Safranin-O. The effect of selenium on osteoblastic cell growth
was also observed. The adherence of MG-63 cells on the coated discs
was detected by staining with Safranin-O. The proportion of covered
area was calculated with imaging software. The tested Staph. aureus strain showed a significantly
reduced attachment on titanium discs with 0.5% (p = 0.011) and 0.2%
(p = 0.02) selenium coating. Our test strain from Staph.
epidermidis showed a highly significant reduction in bacterial
adherence on discs coated with 0.5% (p = 0.0099) and 0.2% (p = 0.002)
selenium solution. There was no inhibitory effect of the selenium
coating on the osteoblastic cell growth. Selenium coating is a promising method to reduce bacterial attachment
on prosthetic material. Cite this article: Bone Joint J 2013;95-B:678–82