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Orthopaedic Proceedings
Vol. 101-B, Issue SUPP_14 | Pages 21 - 21
1 Dec 2019
DIVERSITY OF CUTIBACTERIUM STRAINS INVOLVED IN PROSTHETIC JOINT INFECTIONS AND ABILITY TO PRODUCE BIOFILM: CUTIBACTERIUM ACNES IA1 AND II PHYLOTYPES BELONGING TO SLST-TYPE D1 OR K1 PRODUCE MORE BIOFILM!
Dagnelie M Bemer P d'Epenoux LR Corvec S
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Aim

Cutibacterium acnes (CA) is one of the crucial actors in spine instrumentation or shoulder prosthesis. Its population is subdivided into 6 major phylotypes: IA1, IA2, IB, IC, II and III. Recent methods for discriminating subpopulations within CA phylotypes highlight the predominance of SLST types H1 to 6 or K1 to 20 in bone and joint infection (BJI). The impact of their ability to produce a biofilm during the development of the infection (with resistance / tolerance to antibiotics used for treatment) remains little studied.

Method

The purpose of this study was to determine whether the ability to establish a biofilm varied according to the different subtypes of clinical strains of CA previously characterized and involved in BJI (hip, knee and shoulder prosthesis). The BioFilm ring test (BioFilm Control®) method with index determination, called BFI (BioFilm Index) inversely proportional to the level of biofilm production was used (BFI = 0.00 indicates a high production of biofilm versus BFI = 20.00 indicates zero production). The BFI was determined after 3 h (T3) and 6 h (T6) incubation. The strains used came from patients, 5 belonging to the IA1 phylotype (SLST A1 and D1 types) and 4 to different phylotypes (IA2, IB, II and III).