Deep prosthetic joint infection is a major cause of morbidity. Previous work has shown that infected skin scales shed by members of staff in the operating theatre are the key source of infection. Much attention has been given to the design of ultra clean operating theatres but remarkably little attention has been given to factors controlling skin scale shedding. The aim of this study was to develop a novel method of direct visualisation and quantification of skin scales and to assess the effect of a simple skin care regimen on skin scales. Direct visualisation of the skin surface at high power is difficult due to the depth of surface contours in relation to microscope depth of field. A Zeiss stereo compound Axio-Zoom microscope was used containing a stage on which subjects’ upper or lower limbs could be comfortably placed. A reflected light source allowed direct visualisation of a magnified image of the skin surface. Real-time digital manipulation of multiple z-stacked images on a linked computer created a composite three dimensional image of the skin surface. Density of skin scales was then calculated from this image. We tested the effect of a standardised skincare regime consisting of washing, exfoliation and moisturisation on skin scale density at multiple sites and contralateral controls.Introduction
Patients/Materials & Methods
