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Orthopaedic Proceedings
Vol. 106-B, Issue SUPP_19 | Pages 68 - 68
22 Nov 2024
ANTIBACTERIAL PROPERTIES OF NISIN LAYER-BY-LAYER BASED COATING ON TITANIUM K-WIRES IN GALLERIA MELLONELLA IMPLANT-ASSOCIATED INFECTION MODEL
Mannala G Labat B Ladam G Pascal T Walter N Szymski D Riool M Alt V Rupp M
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Aim

Orthopedic implants play a tremendous role in fixing bone damages due to aging as well as fractures. However, these implants tend to get colonized by bacteria on the surface, leading to infections and subsequently prevention of healing and osteointegration. Recently, Roupie et al. showed that a nisin layer-by-layer based coating applied on biomaterials has both osteogenic and antibacterial properties. The Galleria mellonella larva is a well-known insect infection model that has been used to test the virulence of bacterial and fungal strains as well as for the high throughput screening of antimicrobial compounds against infections. Recently, we have developed an insect infection model with G. mellonella larvae to study implant-associated biofilm infections using Kirschner (K)-wires as implant material. Here, we would like to test the antibacterial capacity of nisin layer-by-layer based coatings on K-wires against Staphylococcus aureus in the G. mellonella larva implant infection model.

Method

Prior to the implantation procedure, G. mellonella larvae are maintained at room temperature on wheat germ in an incubator. The larvae received bare titanium K-wires (uncoated), or either control-coated or nisin-coated K-wires. After one hour, the larvae were injected with 5×105S. aureus bacteria per larva (i.e., hematogenous implant infection model). Next, the larvae were incubated at 37oC in an incubator and the survival of the larvae was monitored for five days. Moreover, the number of bacteria on the implant surface and in the surrounding tissue was determined after 24h of incubation. Further, scanning electron microscopy (SEM) analyses were performed to study the effect of nisin on biofilm formation.

Orthopaedic Proceedings
Vol. 105-B, Issue SUPP_17 | Pages 52 - 52
24 Nov 2023
DIRECT HOSPITAL COSTS PER CASE FOR PERIPROSTHETHIC HIP AND KNEE JOINT INFECTION IN EUROPE: A SYSTEMATIC REVIEW
Szymski D Walter N Hierl K Rupp M Alt V
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Aim

The number of periprosthetic joint infections (PJI) is increasing due to ageing population and increasing numbers of arthroplasty procedures and treatment is costly. Aim of the study was to analyze the direct healthcare costs of PJI in Europe for total hip arthroplasties (THA) and total knee arthroplasties (TKA).

Method

A systematic review in PubMed with search of direct costs of PJI in European countries was performed. Thereby the term cost* AND (infection OR PJI) AND (prosthesis OR knee OR hip OR “TKA” OR “THA” OR arthroplast*) was combined with each European country to detect relevant publications. Publications with definition of performed procedure and joint localization were included into further analysis. The mean value of direct healthcare cost was calculated for the respective joint and the respective operation performed.

Orthopaedic Proceedings
Vol. 105-B, Issue SUPP_17 | Pages 5 - 5
24 Nov 2023
INFECTION AFTER INTRACAPSULAR FEMORAL NECK FRACTURE: DOES ANTIBIOTIC-LOADED BONE CEMENT REDUCE INFECTION RISK AFTER HEMIARTHROPLASTY AND TOTAL HIP ARTHROPLASTY? DATA FROM THE GERMAN ARTHROPLASTY REGISTRY
Szymski D Walter N Krull P Melsheimer O Grimberg A Alt V Steinbrück A Rupp M
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Aim

The aim of this investigation was to compare risk of infection in both cemented and cementless hemiarthroplasty (HA) as well as total hip arthroplasty (THA) following femoral neck fracture.

Methods

Data collection was performed using the German Arthroplasty Registry (EPRD) In HA and THA following femoral neck fracture fixation method was divided into cemented and cementless protheses and paired according to age, sex, body mass index (BMI), and the Elixhauser score using Mahalanobis distance matching.

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