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Orthopaedic Proceedings

Vol. 105-B, Issue SUPP_17 | Pages 63 - 63

24 Nov 2023

MOLECULAR EPIDEMIOLOGY OF COMMUNITY-ONSET METHICILLIN-RESISTANT STAPHYLOCOCCUS AUREUS CAUSING SKIN AND MUSCULOSKELETAL INFECTIONS: AN ALARMING ANTIMICROBIAL RESISTANCE SCENARIO

Prebianchi SB Santos INM Brasil I Charf P Cunha CC Seriacopi LS Durigon TS Rebouças MA Pereira DLC Dell Aquila AM Salles M

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Aim

Community-acquired methicillin-resistant Staphylococcus aureus (CA-MRSA) is commonly associated with serious cases of community-onset skin and musculoskeletal infections (Co-SMSI). Molecular epidemiology analysis of CA-MRSA recovered from skin and soft tissues specimens is lacking in Latin America. This study aimed to identify phenotypic and genotypic features of MRSA isolates recovered from patients presenting Co-SMSI.

Methods

Consecutive MRSA isolates recovered from Co-SMSI of patients admitted from March 2022 to January 2023 in a Brazilian teaching hospital were tested for antimicrobial resistance and characterized by their genotypic features. Identification was carried out by automated method and through MALDI-TOF MS. Antimicrobial susceptibility was tested by disk diffusion, broth microdilution and E-test strips for determination of the minimal inhibitory concentration (MIC) according to recommendations from the Brazilian Committee on Antimicrobial Susceptibility Testing (BrCAST) and European Committee on Antimicrobial Susceptibility Testing (EUCAST). Gene mecA characterization and Sccmec typing were performed by multiplex polymerase chain reaction (PCR) assay, and gene lukF detection by single PCR. Patients were prospectively followed up for two months, in order to determine their clinical characteristics and outcomes.

Orthopaedic Proceedings

Vol. 105-B, Issue SUPP_17 | Pages 6 - 6

24 Nov 2023

MICROBIOLOGIC EPIDEMIOLOGY OF HIP PROSTHETIC JOINT INFECTIONS IN ELDERLY PATIENTS CAUSED BY MULTIDRUG-RESISTANT GRAM-NEGATIVE BACTERIA: A RETROSPECTIVE COHORT

Soares F Santos INM Seriacopi LS Durigon TS Cunha CC Dell Aquila AM Salles M

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Currently, gram-negative bacteria (GNB), including multidrug-resistant (MDR-GNB) pathogens, are gaining importance in the aetiology of prosthetic joint infection (PJI). To characterize the antimicrobial resistance patterns of Gram-negative bacteria (GNB) causing hip prosthetic joint infections in elderly patients treated at a Brazilian tertiary academic hospital.

Method

This is a retrospective, cross-sectional study of patients over 60 years of age undergoing hip arthroplasty from 2018 to 2023 at a tertiary academic trauma, which were diagnosed with hip prosthetic joint infection. PJI diagnosed was based on EBJIS criteria, in which intraoperative tissue cultures identified the pathogens. Demographics, reason for arthroplasty, type of implant and susceptibility patterns using disk diffusion method were analysed.

Orthopaedic Proceedings

Vol. 104-B, Issue SUPP_10 | Pages 59 - 59

1 Oct 2022

COMPARATIVE PHENOTYPIC AND GENOMIC FEATURES OF STAPHYLOCOCCI FROM SONICATION FLUID OF ORTHOPAEDIC IMPLANT-ASSOCIATED INFECTIONS WITH POOR OUTCOME

Santos INM Kurihara MNL Santos FF Valiatti TB d. Silva JTP Pignatari ACC Salles M

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Aim

S. aureus and S. epidermidis remain the leading biofilm-forming agents causing orthopedic implant-associated infections (OIAI), but other coagulase-negative Staphylococcus (CoNS) with clinical importance is emerging. Besides, few studies have assessed specific genomic traits associated with patient outcome. This is a preliminary descriptive study of phenotypic and genomic features identified in clinical isolates of S. aureus and CoNS isolates recovered from OIAIs patients that progressed to treatment failure.

Methods

Ten isolates were identified by matrix-time-of-flight laser-assisted desorption mass spectrometry (MALDI-TOF-MS) and tested for antibiotic susceptibility and biofilm formation. Genotypic characteristics, including, MLST (Multi Locus Sequence Typing), SCCmec typing, virulence and resistance genes were assessed by whole-genome sequencing (WGS) that was performed on an Illumina HiSeq 2500 platform. Bioinformatics analyzes were performed using CGE, PATRIC, VFDB, CARD RGI, SnapGene, BLAST, and PubMLST. S. aureus (215, 260 and 371) isolates belonged to CC5 (ST5 and ST105, spa type t002) and carried SCCmec type I (1B), II (2A) and V(5C2), respectively.

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