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Orthopaedic Proceedings
Vol. 106-B, Issue SUPP_19 | Pages 69 - 69
22 Nov 2024
COMBINATION OF SONICATION WITH DITHIOTHREITOL TREATMENT DOES NOT IMPROVE BACTERIAL DISLODGING FROM BIOFILM IN AN IN VITRO MODEL
De Vecchi E Riccardi M Mastroianni N
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Aim

Diagnosis of prosthetic joint infection are often complicated by the presence of biofilm, which hampers bacteria dislodging from the implants, thus affecting sensitivity of cultures. In the last 20 years several studies have evidenced the usefulness of implant sonication to improve microbial recovery from biofilm formed on inert substrates. More recently, treatment of prosthetic joints and tissues with Dithiothreitol, a sulphur compound already used in routine diagnostic workflow for fluidification of respiratory samples, has proved to be not inferior to sonication in microbiological diagnosis of prosthetic joint infections.

This study aimed to evaluate if the combination of the two treatments could further improve microbial retrieval from biofilm in an in vitro model.

Method

Three isolates of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lugdunensis, Eschericha coli and Pseudomonas aeruginosa responsible of prosthetic joint infections were used. They were grown onto 3 titanium discs (20 mm diameter) and incubated in 3 sterile plastic containers with 15 mL of Triptyc Soy Broth. After overnight incubation, not adhered cells were removed and fresh broth was added to each sample. After 48 hours incubation, the exausted broth was removed and one sample was used for sonication, one for treatment with 0,1% (v:v) Dithiothreitol and one treated with Dithiothreitol followed by sonication. Treated fluids were plated on Muller Hinton Agar plates for colony count.

One-way ANOVA analysis was performed to evidence statistical differences between treatments.

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