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Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_7 | Pages 144 - 144
1 May 2016
BACTERIAL BIOFILM FORMATION ON ANTIBIOTIC-LOADED BONE CEMENT. A PRELIMINARY IN VITRO STUDY
Galasso O Balato G Catania M Gasparini G Mariconda M
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Introduction

The use of antibiotic-loaded polymethylmethacrylate bone-cement spacers during two-stage exchange procedures is the standard in the treatment of patients with delayed prosthetic joint infection. The real antimicrobial activity of these spacers is unclear because the adherence of bacteria to cement might result in clinical recurrence of infection. The purpose of the study is to evaluate the in vitro formation of Pseudomonas Aeruginosa (PA) and Staphylococcus spp. biofilm on antibiotic-loaded bone cement.

Materials and methods

Cement disks (diameter = 6 mm) impregnated with gentamicin and colistin were submerged in bacterial suspensions of Methicillin-resistant Staphylococcus Aureus(MRSA), Staphylococcus epidermidis (SE), and PA. Negative controls (specimen disks without antibiotic) were similarly prepared. Biofilm formation was visualized by confocal scanning laser microscopy (CSLM), after staining the discs with the live/dead BacLight viability stain containing SYTO 9 dye and propidium iodide. Images from five randomly selected areas were acquired for each disc. Sequential optical sections of 2 µm were collected in sequence along the z-axis over the complete thickness of the sample. The resulting stacks of images were analyzed, quantified and rendered into three-dimensional (3D). The biofilm thickness on antibiotic bone cement compared with the controls was automatically evaluated.

Orthopaedic Proceedings
Vol. 95-B, Issue SUPP_34 | Pages 315 - 315
1 Dec 2013
Long-Term Follow-Up Results of Total Hip Arthroplasty
Galasso O Mariconda M Sticozzi M Gasparini G
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BACKGROUND:

Modern total hip arthroplasty (THA) has shown a great improvement in pain, function and range of motion of patients but data on patients' quality of life after this surgery as assessed by validated tools are lacking.

METHODS:

In this cohort study we evaluated the quality of life and functionality of 250 patients an average of 16 years (11–23) after THA using the SF-36 questionnaire, the Harris Hip Score, the WOMAC score, the Functional Comorbidity Index, and a study specific questionnaire. Models of multiple stepwise linear and logistic regression analysis were constructed to evaluate the relationships between the explanatory variables and the functional outcomes.