With the success of the medial parapatellar approach (MPA) to total knee arthroplasty (TKA), current research is aimed at reducing iatrogenic microneurovascular and soft tissues damage to the knee. In an effort to avoid disruption to the medial structures of the knee, we propose a novel quadriceps-sparing, subvastus lateralis approach (SLA) to TKA. The aim of the present study is to compare if a SLA can provide adequate exposure of the internal compartment of the knee while reducing soft tissue damage, compared to the MPA. Less disruption of these tissues could translate to better patient outcomes, such as reduced post-operative pain, increased range of motion, reduced instances of patellar maltracking or necrosis, and a shorter recovery time. To determine if adequate exposure could be achieved, the length of the skin incision and perimeter of surgical exposure was compared amongst 22 paired fresh-frozen cadaveric lower limbs (five females/six males) which underwent TKA using the SLA or MPA approach. Additionally, subjective observations which included the percent of visibility of the femoral condyles and tibial plateau, as well as the patellar tracking, were noted in order to qualify adequate exposure. All procedures were conducted by the same surgeon. Subsequently, to determine the extent of soft tissue damage associated with the approaches, an observational assessment of the dynamic and static structures of the knee was performed, in addition to an examination of the microneurovascular structures involved. Dynamic and static structures were assessed by measuring the extent of muscular and ligamentus damage during gross dissection of the internal compartment of the knee. Microneurovascular involvement was evaluated through a microscopic histological examination of the tissue harvested adjacent to the capsular incision. Comparison of the mean exposure perimeter and length of incision was not significantly different between the SLA and the MPA (p>0.05). In fact, on average, the SLA facilitated a 5 mm larger exposure perimeter to the internal compartment, with an 8 mm smaller incision, compared to the MPA, additional investigation is required to assert the clinical implications of these findings. Preliminary analysis of the total visibility of the femoral condyles were comparable between the SLA and MPA, though the tibial plateau visibility appears slightly reduced in the SLA. Analyses of differences in soft tissue damage are in progress. Adequate exposure to the internal compartment of the knee can be achieved using an incision of similar length when the SLA to TKA is performed, compared to the standard MPA. Future studies should evaluate the versatility of the SLA through an examination of specimens with a known degree of knee deformity (valgus or varus).