Advertisement for orthosearch.org.uk
Results 1 - 3 of 3
Results per page:
Applied filters
Include Proceedings
Dates
Year From

Year To
Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXVII | Pages 38 - 38
1 Jun 2012
Miller N Swindle K Cook S Dunn J Smith G Justice C
Full Access

Introduction

Clinical studies have shown distinct differences in later-onset idiopathic scoliosis (IS) between men and women, including curve severity, stiffness, and ease of operative intervention. Therefore, significant scoliosis in men was used as criteria to create a phenotypical subset of families with IS. The goal of this study is to identify genetic determinants that relate specifically to men with a scoliotic curvature of 30° or more.

Methods

We identified 25 families (208 individuals) in which a male was diagnosed with 30° or more IS curvature in adolescence. 123 individuals were affected (48 male; 75 female), and 85 were unaffected (45 male; 40 female). Initially, a genomic screen was done with a modified CHLC (version 9) marker set. After initial linkage analyses, the group underwent finemapping with a custom single-nucleotide polymorphism (SNP) panel and ABI Taqman methodology on an ABI 377 platform. The initial genome-wide screen and subsequent analyses were analysed by model-independent linkage analysis with SIBPAL (SAGE, version 5).


Orthopaedic Proceedings
Vol. 90-B, Issue SUPP_III | Pages 435 - 435
1 Aug 2008
Miller N Marosy B Roy-Gagnon M Doheny K Pugh E Wilson A Justice C
Full Access

Introduction: Familial idiopathic scoliosis (FIS) is a complex genetic disorder potentially resulting from multiple genetic interactions and variants. A previous genome wide screen in a large population of families with FIS followed by fine mapping utilizing STRP’s identified and narrowed critical regions on chromosomes 9 and 16. A high density SNP map was then designed across these regions. This array was then assayed within the same population in an effort to link and/or associate specific genetic intervals or candidate genes with the expressed phenotype.

Methods: A sample of families with IS (202 families, 1198 individuals) was recruited with IRB approval and underwent a genomic screen. Results were analysed by model-independent linkage analysis (SIBPAL). Following initial analyses, families were then stratified according to mode of inheritance. 101 families (550 individuals) represented an autosomal dominant mode of heritability and underwent fine mapping in the candidate regions.

Custom SNP pools were designed for the candidate regions at a density of 1 SNP/58Kb. DNA from 550 individuals (AD group) were genotyped using the Illumina platform. A total of 1536 SNP markers were attempted, of which 1324 were released; 519 SNPs were genotyped on 9q32-24 and 805 SNPs genotyped on 16p12-q22. The map was generated using NCBI dbSNP chromosome report on Build 34. Overall missing rate was 0.06%; the overall duplicate error rate was 0.05%.

FIS was analysed both as a qualitative trait with an arbitrary threshold, and as a quantitative trait, or the degree of lateral curvature. Model independent sib-pair linkage analysis was performed on the subsets (SIBPAL, S. A. G. E. v4.5).

Results:

Chromosome 9: Multipoint model-independent qualitative analysis (threshold at ten degrees) did not result in any p values of < 0.05. When the threshold was set at 30 degrees, several regions with p values of < 0.005 were observed. One region spanned 10 Mb, and coincides with the region found to be most suggestive of linkage at the 0.05 level for the quantitative analysis which was 6 Mb in length.

Chromosome 16: Multipoint model-independent qualitative analysis (threshold at ten degrees) resulted in a region spanning 23Mb with p values of < 0.05. The region included both regions adjacent to the centromere. When analysis was performed at a threshold of 30 degrees, the p values became more significant within a region of 30 Mb significant at the 0.05 level. The region best defined at a 0.01 level was located in an 8 Mb region on the q arm.

Discussion: The current work has significance in the stepwise confirmation and narrowing of genomic regions which are potentially meaningful in the aetiology of FIS. Stratification of the initial sample into subgroups, initially by heritability and then by threshold of disease resulted in heightened significance at specific markers demonstrating the heterogeneity of this disorder. Ultimately, the independent association of genetic loci and this disorder will enhance the ability to elucidate prognosis, counsel patients, and guide therapeutic plans.


Orthopaedic Proceedings
Vol. 90-B, Issue SUPP_III | Pages 435 - 435
1 Aug 2008
Marosy B Vu C Zorn A Nzegwu N Justice C Miller N
Full Access

Introduction: Classification systems in relation to scoliosis have been a hallmark for the clinician in the development of therapeutic options. The triple curve pattern with three distinct lateral curvatures of approximately equal severity has been recognised as distinct and, potentially, unique in its presentation. From a large population of families with FIS, a subpopulation of families with a triple curve pattern was evaluated in order to determine if this curve pattern is distinct on a genetic level.

Methods: With IRB approval, a sample of families with FIS (202 families, 1198 individuals) were recruited and underwent a genomic screen. The results were analysed using a model independent linkage analysis (SIBPAL). A subgroup of FIS families with at least one member having a triple curve was identified (six families, 32 individuals). After initial linkage analysis, the group underwent further fine mapping analysis utilising a battery of SNPs.

Results: Analysis of the data from the genomic screen on the triple curve subgroup revealed significant areas on chromosome 10 when analysed qualitatively and quantitatively in either a single-point or multipoint fashion.

Conclusion: The utilization of clinical data to discern potential relevance of specific genetic loci in the aetiology of FIS has resulted in an area on chromosome 10 that is significant (p < 0.01). The relatively small population of families within this subgroup coupled with the strength of the data suggests a unique genetic etiological factor associated with the formation of a triple curve in FIS.