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Orthopaedic Proceedings
Vol. 98-B, Issue SUPP_23 | Pages 34 - 34
1 Dec 2016
Gbejuade H Hidalgo-Arroy A Sayers A Leeming J Lovering A Blom A Webb J
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Aim

To evaluate the ability of different combinations of antibiotic loaded cement to inhibit bacteria growth and biofilm formation.

Method

Cement beads were aseptically prepared using Palacos R (plain 40g PMMA cement) or Palacos R+G (40g PMMA cement containing industrially added 0.5g of gentamicin), with or without supplementary antibiotics as follows: Palacos R; Palacos R+G; Palacos R plus 1g / 2g daptomycin; Palacos R+G plus 1g / 2g of daptomycin; Palacos R plus 1g / 2g vancomcyin; and Palacos R+G plus 1g / 2g vancomycin. After production, each antibiotic loaded acrylic cement (ALAC) combination was allocated into two groups (group 1 and 2).

The group 2 cement beads were initially eluted in broth at 37o C for 72hours then transferred to fresh broth containing a known concentration of bacteria. The group 1 samples were not eluted but directly immerse in culture broth containing bacteria. All samples were thereafter incubated at 37oC for 24 hours. After incubation, group 1 samples were visually assessed for bacterial growth, while for the group 2 samples, biofilm formation were quantified using ultrasonication and viable bacteria counting technique. Three proficient biofilm forming Staphylococcus epidermidis bacterial strains (1457, 1585-RA and 5179-R1) were used for all experiments and the bacteria counts were expressed as colony forming units / ml (CFU/ml).


Orthopaedic Proceedings
Vol. 96-B, Issue SUPP_11 | Pages 35 - 35
1 Jul 2014
Gbejuade H Lovering A Hidalgo-Arroyo A Leeming J Webb J
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Summary Statement

Conventional culture techniques have poor sensitivity for detecting bacteria growing in biofilms, which can result in under-diagnosis of infections. Sonication of biofilm colonised orthopaedic biomaterials can render bacteria in biofilm more culturable, thereby improving diagnosis of orthopaedic implant infections.

Introduction

Prosthetic joint infection (PJI) is a potentially devastating complication in arthroplasty. Biofilm formation is central to PJI offering protection to the contained bacteria against host defence system and antimicrobials. Orthopaedic biomaterials generally have a proclivity to biofilm colonisation. Conventional culture technique has a low sensitivity for detecting bacteria in biofilm. Sonication can disrupt bacteria biofilms aggregations and dislodge them from colonised surfaces, rendering them culturable and consequently improve the diagnosis of otherwise culture-negative PJI. We investigated the effect of ultrasonication on biofilms adherent to poylmethylmethacrylate PMMA cement.


Orthopaedic Proceedings
Vol. 88-B, Issue SUPP_II | Pages 330 - 331
1 May 2006
García-Mata S Gozzi S Ibarlucea E Hidalgo A
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Introduction: Calcaneal lengthening osteotomy as a treatment for severe flat foot was described by Evans (1975) and his indications were expanded by Mosca (1995).

Materials and methods: We reviewed 28 feet operated on in 21 patients with neuromuscular processes (17) and with flat foot-valgus associated with congenital short Achilles tendon (11). The mean age was 12.2 (6–18) and mean follow-up was 7.3 years (4–11). In all cases the associated Achilles tendon was lengthened. We evaluated the subjective and objective clinical results (Kitaoka, 1994) and the radiological parameters.

Results: Subjective clinical: excellent and good 89.28% (100% in neuromuscular, 72.7 in short Achilles tendon). Objective clinical: (preop 64.25, postop 90.06 at 3 years, 86.13 at 7 years). The worsening of outcomes was due to three fair or poor results in the short Achilles tendon group (one required triple bone fusion).

All but one presented complete subtalar mobility. Normal alignment in all but one, with slight valgus; none in varus.

Conclusions: This surgical procedure gave excellent long-term results: subjective, nearly 90% excellent; objective: radiological anatomical correction, subtalar and tarsal mobility maintained. Its outcome was comparatively worse in the short Achilles tendon group than in the neuromuscular cases.