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Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXXIX | Pages 187 - 187
1 Sep 2012
Lui D Hayes DB Seamus M Kenny P McElwain J Bennett D
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Aims

Hip fractures pose a significant burden on the healthcare system. Hyperglycaemia and a state of Type 2 diabetes exists post operatively. Being normoglycaemic has well documented benefits. Pre operative carbohydrate loading has been shown to have two good effects. It decrease hyperglycaemia post operatively and allows the patient to undergo less strict fasting protocols. Insulin resistance to date has not been examined in these patients and this was determined using a validated formula (HOMA/IR).

Methods

Three trauma hospitals were enrolled and patients with hip fractures requiring operative fixation were enlisted. Exclusion criteria: diabetic patients and inability to imbibe. 100 neck of femur fractures were examined. 46 patients were fasted normally. 32 test patients were given a carbohydrate rich drink pre operatively the night before surgery and in the morning up to 2 hours prior to surgery. 22 patients were excluded. Serum random glucose and insulin levels were taken on admission. Fasting serum glucose and insulin levels were taken on day one post operatively.


Orthopaedic Proceedings
Vol. 92-B, Issue SUPP_IV | Pages 617 - 617
1 Oct 2010
Lui D Hayes DB Condron C
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Introduction: Strict maintenance of normoglycaemia with intensive insulin therapy in the critically ill surgical patients has helped to reduce morbidity and mortality by almost 50%. The notion that insulin may act independently of glucose as an anti inflammatory agent is of interest. Orthopaedist manipulate the inflammatory cascade through the practice of Damage Control Surgery. By delaying surgery they prevent a second hit in the Systemic Inflammatory Response Syndrome (SIRS) and attenuate excessive inflammation which may lead to Multiorgan Failure (MOF). An insulin infusion is a novel method of modulating the inflammatory cascade through the strict control of hyperglycaemia.

The role of neutrophils and endothelium are an integral part of the inflammatory cascade. Our aim was to investigate whether insulin had an independent effect on endothelial cell activation.

Aim: We hypothesise that insulin, independent of glucose, has a cytoprotective effect on the endothelium as an anti inflammatory agent.

Methods: We subjected human umbilical vein endothelial cells (HUVEC) to normoxia, hypoxia and hypoxia reoxygenation to simulate trauma. These 3 groups were incubated with insulin at 0uU/ml, 10uU/ml, 50uU/ml and 100uU/ml for 24h and 48h normoxia and hypoxia. For the hypoxia reoxygenation study HUVEC were exposed to 24h of hypoxia and then 24h of reoxygenation. Proliferation of endothelial cells was measured using an MTT study.

Results: Our experiment shows that hypoxia reduces HUVEC proliferation. Results show that treatment with 50uU/ml insulin for 24 hours attenuates the effect of hypoxia. This suggests that insulin at post prandial, physiological levels, in non diabetics has a cytoprotective effect on endothelial cells. This was significant in hypoxic conditions in a dose dependent manner.

Conclusions: Hypoxia simulates injury and when injury occurs it activates an inflammatory response which could lead to SIRS. It has not previously been investigated how insulin acts as an anti inflammatory mediator in the control of hyperglycaemia. We can conclude that insulin may act to protect the endothelium, independent of glucose, under hypoxia and hypoxia reoxygenation conditions.