Wolff's Law proposes that trabecular bone adapts in response to mechanical loading and that trabeculae align with the trajectory of predominant loads. The current study is aimed to investigate trabecular orientation in the tibia in patients with osteoarthritis of the knee. Consistent with Wolff's Law, it was hypothesised that orientation would reflect the mechanical loading of the joint and hence that there would be a correlation between the trabecular orientation and the mechanical axis of the lower limb. 51 anonymised radiographs from patients with osteoarthritis were analysed using ImageJ (National Institute of Health). Each patient had both a standard anteroposterior radiograph of the knee and a long leg view taken while weight bearing. For each anteroposterior radiograph, the angle of the femoral shaft and tibial shaft were measured. The femoral shaft – tibial shaft (FS -TS) angle was then calculated as the difference between the two, as described by Sheehy et al. (2011). A medial rectangle was selected with the top, bottom, medial and lateral borders being the sclerotic bone, the growth line, the bone edge and the centre of the medial tibial spine. Corresponding measurements were done on the lateral side. Trabecular orientation of both areas was measured using OrientationJ (an ImageJ plugin). In all cases the medial and lateral orientation angles were expressed relative to the angle of the tibial shaft. The mechanical axis of the lower limb was measured from the full length radiographs by calculating the angle formed by the femoral and tibial axes, as described by Goker and Block. All measurements were done independently by two observers, SAS and SL.Introduction
Methods
