Duchenne muscular dystrophy (DMD) is a prevalent childhood neuromuscular disease characterized by progressive skeletal and cardiac muscle degeneration due to dystrophin protein deficiency. Despite ongoing drug development efforts, no cure exists, with limited success in preclinical studies. To expedite DMD drug development, we introduce an innovative organ-on-a-chip (OOC) platform. This microfluidic device sustains up to six 3D patient-derived skeletal muscle tissues, enabling real-time evaluation of anti-DMD treatments. Our in vitro model recreates myotube integrity loss, a hallmark of DMD, by encapsulating myogenic precursors in a fibrin-composite matrix using a PDMS casting mold. Continuous contractile regimes mimic sarcolemmal instability, monitored through tissue contractibility and Creatine Kinase (CK) levels—an established marker of muscle damage. We further enhance our platform with a nanoplasmonic CK biosensor, enabling rapid, label-free, and real-time sarcolemmal damage assessment. Combining these elements, our work demonstrates the potential of OOCs in accelerating drug development for DMD and similar neuromuscular disorders.

Introduction: Hip Surgical Dislocation (SHD) according the technique described by Ganz et al. is a safe and powerful tool to access intra-articular hip pathology in adults. Some indications may also arise in younger patients to correct slipped capital femoral epiphysis or femoral neck deformities

Materials and Methods: From 2004 to 2008 we have selected 45 patients on whom the procedure was done to treat femoroacetabular impingement (FAI). The average follow up time is 3 years, and patient mean age 26 years. The indications for SHD were:

mixed FAI in 26 cases,

42 hips where graded as Tönnis 0 and 2 as Tönnis 1. All patients where evaluated according to the non arthritic hip score (NAHS – McCarthy et all) before and after the surgery at 3, 6, 12, 24 and 36 months. Osyrix^® software was used to measure radiographic parameters. The numeric variables where treated using SPSS for windows (paired t student test).

Surgical Technique: In all 45 cases we did SHD, acetabular and/or femoral head neck junction trimming and labrum refixation. In half cases an anterior step trochanteric osteotomy was done and in 7 cases additional relative neck lengthening was performed.

Results: The average alfa angle measured in the standard crosstable view x ray was 72° before surgery and 36° after surgery (p=0,0001). The NAHS before surgery was 40,8 average: 9,71 – pain; 6,9 – symptoms; 9 – function and 6,9 – activities and after surgery 76,38 average (p= 0,0001) 17,5 – pain (p= 0,0001); 12,9 – symptoms (p= 0,0001); 16 - function (p= 0,0001) and 14,9 - activities (p= 0,0001). All patients improved motion, specially flexion, internal rotation (p= 0,0001). The results did not differ significantly in the patients who had a trochanteric anterior step osteotomy. One patient had a total hip replacement for ongoing osteoartrithis

We had no avascular necrosis so far and no neurovascular damage. Trochanteric screw removal was done in 3 cases for local irritation. We had 2 capsule adhesions, released shortly after using arthroscopy.

Conclusions: SHD is a demanding technique with full access to femoral head and acetabular deformities as well as cartilage or labral tears. It can be done safely with a low complication rate. The best results are achieved in young patients without degenerative cartilage and significant labrum changes. Hip degenerative changes contraindicates this procedure. Modification of trochanteric osteotomy does not seem to influence results

Material & Methods: Twenty three patients (15 male; 8 female) with an average 30 years old (16–63) presented with a posttraumatic multidirectional instability of the distal RU joint. In two occasions instability was associated to a malunited distal radial fracture which was corrected with a osteotomy. Through a dorsoulnar approach, the RU joint in all cases was inspected and found with no cartilage defects that could preclude this intervention. Also in all occasions there was an unrepairable peripheral TFCC detachment. In 11 cases the palmaris longus tendon was utilized, while in one the flexor superficialis of the ring finger was used. The graft was passed through an anteroposterior tunnel in the distal-medial edge of the radius and attached into another tunnel in the basistyloid fovea. The forearm was then immobilized in neutral pronosupination for 4–6 weeks, followed by appropriate physiotherapy.

Results: At an average 18 months follow-up (6–36) 21 patients regained radioulnar stability (symmetrical passive displacement of the joint relative to the contralateral side). In two there was a limitation of more than 25° pronosupination. All 15 patients with a follow-up longer than one year had returned to their previous activities with a less than 20% loss of grip strength.

Conclussion: Tendon reconstruction as suggested by Adams& Berger is anatomically sound, and it has shown promising short term results, certainly superior to the results obtained with other soft-tissue reconstructions.