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Orthopaedic Proceedings
Vol. 94-B, Issue SUPP_XXXVII | Pages 552 - 552
1 Sep 2012
Lustig S Laurent F Bouaziz A Blanc-Pattin V Rasigade J Ferry T Tigaud S Neyret P
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Introduction

Rapid identification of bacteria from extemporaneous samples would greatly help management of prosthesis joint infection. The aim of the present retrospective study was to evaluate a new molecular assay (GeneXpert MRSA-SA SSTI (Cepheid)) for detecting Staphylococcus aureus (SA) and methicillin resistance directly from bone and joint samples in less an hour (58 minutes).

Material et method

Retrospective study using 91 frozen samples (76 patients) of joints (n=24), bone biopsies (n=42) and tissue biopsies (n=25):

SA positive samples: n=72 (methicillin susceptible SA (MSSA), n=63; methicillin resistant MRSA, n=9)

SA positive samples: n=19

The results were compared with routine results (culture in solid and liquid medium, identification and susceptibility test) from each participating lab.