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Orthopaedic Proceedings
Vol. 88-B, Issue SUPP_I | Pages 51 - 51
1 Mar 2006
Nieuwenhuis J Malefijt de JW Gosens T Bonnet M
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Because we encountered a high failure rate of the acetabular component of the uncemented, hydroxy-apatite coated, Omnifit total hip prosthesis (Osteonics corporation, Allendale, NJ, USA), we conducted a retrospective study of 418 consecutive total hip arthroplasties with a mean follow up of 60 months.

The results of 418 hydroxy-apatite coated, uncemented Omnifit total hip arthroplasties, conducted between 1989 and 1996 were evaluated. Two different acetabulum cups were used: 145 screwcups with one central screwhole, and 273 press fit (PF) cups with several screwholes. The internal geometry of these cups and the fixation of the polyethylene insert in the metal cups were identical. In 339 arthroplasties a 32-mm femur-head was used, in 79 a 28-mm head. Patients’ records and x-rays were evaluated for clinical findings, polyethylene (PE) wear, acetabular and femoral osteolysis and findings during revision surgery.

Revision surgery was performed in 73 patients, mainly because of symptomatic acetabular osteolysis (79%). Mean PE wear was 0.16 mm/year (0.19 mm in PF cups, 0.11 mm in screwcups). Acetabular osteolysis was found to be present in178 hips (70 screwcups and 108 PF cups). In both cupdesigns the osteolysis was mainly found around the screwholes of the metal cups. During revision surgery these osteolytic defects were a lot larger than suggested by x-ray imaging. In 22.6% of the hips osteolysis was also present in the proximal femur.

Kaplan-Meier survival analysis showed, after 6 year follow up, a better survival for of the screwcup (96%, confidence interval 93–99%) than the PF cup (66%, 95%CI 56–77%).

We hypothesized that this specific combination of metal cup and polyethylene insert -possibly due to an insufficient fitting- is responsible for the migration of polythylene wear particles through the screwholes in the metal cup, causing acetabular osteolysis and neces-satating revision surgery.

For this reason we abandonned the use of this type of uncemented acetabular component.


Orthopaedic Proceedings
Vol. 86-B, Issue SUPP_I | Pages 48 - 48
1 Jan 2004
Bandelier M Denormandeie P Denys P Sapena R Enouf D Youssefian T Blondeau Y Bonnet M Smail DB Mailhan L Judet T
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Purpose: Few studies have been devoted to neurogenic paraosteoarthorpathy (PAOn). We characterised the expression of genes specific for osteoblastic and chon-drocytic phenotypes using the osteomy wedge and non-mineralised tissue near the osteotome.

Material and methods: Osteotomy fragments and non-mineralised tissue near the osteotomy were obtained during surgery performed in 25 patients. The explants were cultured for 56 days. We searched for the messenger RNA of the principal markers of osteoblastic, chon-drocytic, and adipocytic phenotypes, as well as certain specific proteins. Serial cryotome sections were stained for histology and immunolabelling tests.

Results: Cells issuing from the osteotomy fragment and neighbouring tissues formed structures that miner-alised in culture. The following osteoblast markers were observed: alkaline phosphatase (bone isoform), osteo-calcin, Cbfa1, type 1 collagen; for chondrocytes: type II collagen, aggrecane; type X collagen as well as VEGT demonstrating the presence of hypertrophic chondrocytes.The adipocyte-specific transcription factor PPAR 2 was also found in the two cultures. The proportions and chronological expression of these markers were slightly different for the two tissues. Ex vivo study demonstrated the typical sequence of enchondral type bony formation from non-osseous cell populations.

Discussion: This work provided the first characterisation of non-mineralised tissue near osteotomy. It also provided clear indications concerning the history of ectopic bone formation. The osteochondrogenic potential of connective tissue lying close to an osteotomy has not been reported previously. The persistence of this potential could explain recurrence after resection. The observation that this potential is suppressed in vivo but expressed in vitro opens a new avenue of research concerning the mechanisms controlling bone formation.

Conclusion: The culture model developed in this study provides a means of studying factors determining the outcome of cell populations implicated in the formation of neurogenic paraosteoarthropathies.