Meniscal injuries affect over 1.5 million people across Europe and the USA annually. Injury greatly reduces knee joint mobility and quality of life and frequently leads to the development of osteoarthritis. Tissue engineered strategies have emerged in response to a lack of viable treatments for meniscal pathologies. However, to date, constructs mimicking the structural and functional organisation of native tissue, whilst promoting deposition of new extracellular matrix, remains a bottleneck in meniscal repair. 3D bioprinting allows for deposition and patterning of biological materials with high spatial resolution. This project aims to develop a biomimetic 3D bioprinted meniscal substitute.

Meniscal tissue was characterised to effectively inform the design of biomaterials for bioprinting constructs with appropriate structural and functional properties. Histology, gene expression and mass spectrometry were performed on native tissue to investigate tissue architecture, matrix components, cell populations and protein expression regionally across the meniscus. 3D laser scanning and magnetic resonance imaging were employed to acquire the external geometrical information prior to fabrication of a 3D printed meniscus. Bioink suitability was investigated through regional meniscal cell encapsulation in blended hydrogels, with the incorporation of growth factors and assessed for their suitability through rheology, scanning electron microscopy, histology and gene expression analysis.

Meniscal tissue characterisation revealed regional variations in matrix compositions, cellular populations and protein expression. The process of imaging through to 3D printing highlighted the capability of producing a construct that accurately replicated meniscal geometries. Regional meniscal cell encapsulation into hydrogels revealed a recovery in cell phenotype, with the incorporation of growth factors into the bioink's stimulating cellular re-differentiation and improved zonal functionality.

Meniscus biofabrication highlights the potential to print patient specific, customisable meniscal implants. Achieving zonally distinct variations in cell and matrix deposition highlights the ability to fabricate a highly complex tissue engineered construct.

Acknowledgements: This work was undertaken as part of the UK Research and Innovation (UKRI)-funded CDT in Advanced Biomedical Materials.

Introduction: Leg length discrepancy (LLD) following total hip arthroplasty (THA) can lead to disappointed patients, increased dislocation, increased wear and suboptimal function. It is one of the commonest reasons for litigation following THA in the United States. The purpose of this study was to radiologically and functionally assess the efficacy of a simple technique for intra-operative leg length assessment during THA via a posterior approach.

Materials & Methods: This technique was undertaken in 50 consecutive THAs. The pre-and 3 month postoperative LLD was measured on standing AP pelvis radiographs. The results were compared with 50 THAs performed by the same surgeons without using the technique. Pre-and post-operative OHS and UCLA scores were recorded in both groups.

Results: In the control group the mean pre- and postoperative LLD was 9.38 mm and 7.75mm respectively. In the new technique group the mean pre-operative LLD was 11.37 and the post-operative LLD was 1.70mm. The final LLD was significantly less in the new technique group (p< 0.001). Fifteen patients in the control group and three patients in the new technique group had post–operative lengthening. Oxford Hip and UCLA score improvement in new technique group was greater than in the control group (p< 0.05).

Discussion: The technique we introduced to assess leg length intra-operatively has shown to be safe, reliable and accurate. We have nonetheless demonstrated much greater accuracy at providing equal leg lengths and improved functional outcome using this new technique.