Aim. Antibiotic prophylaxis is central in preventing postoperative spine infections, yet knowledge of clinical spine tissue antibiotic concentrations remains limited. Pooled postoperative spine infection rates are constant (approximately 3%), resulting in severe patient morbidity, mortality, and prolonged hospitalization. Current antibiotic dosing regimens often involve fixed doses based on empirical knowledge, surrogate measures (plasma samples), non-clinical evidence (experimental models), and inferior methodology (tissue specimens). Therefore, personalized antibiotic dosing may be the future of antibiotic prophylaxis to prevent postoperative infections, especially implant infections. The aim was to continuously evaluate intra- and postoperative cefuroxime target spine tissue concentrations in long-lasting spine surgery after personalized dosing by repeated weight-dosed intravenous administrations. Method. Twenty patients (15 female, 5 male) scheduled for long-lasting spine deformity surgery with hypotensive anaesthesia were included; median age (range): 17.5 years (12-74), mean BMI (range): 22.2 (16.2-37.7), and mean surgery time (range): 4h 49min (3h 57min-6h 9min). Weight-dosed cefuroxime (20 mg/kg) was administered intravenously to all patients on average 25 min before incision and repeated after 4 hours. Microdialysis catheters were placed for sampling of cefuroxime concentrations in vertebral bone (only intraoperative sampling), paravertebral muscle, and subcutaneous tissue as soon as possible after surgery start. Upon wound closure, two additional catheters were placed in the profound and superficial part of the wound. Microdialysis and plasma samples were obtained continuously intra- and postoperative for up to 12 hours. The primary endpoint was (based on cefuroxime time-dependent efficacy) the time with cefuroxime concentrations above the clinical breakpoint minimal inhibitory concentration for Staphylococcus aureus of 4 µg/mL in percentage (%fT>MIC4) of. (a). patients’ individual surgery time,. (b). first dosing interval (0-4 hours),. (c). second dosing interval (4-12 hours). Results. Mean cefuroxime %fT>MIC4 (range) of:. (a). patients’ individual surgery time was 100% (100-100%) in all investigated tissues. (b). the first dosing interval was 93% (93-93%) in vertebral bone, paravertebral muscle, subcutaneous tissue, and 99% (99-100%) in plasma. (c). the second dosing interval was 87% (52-100%) in paravertebral muscle, 89% (52-100%) in subcutaneous tissue, 91% (71-100%) in the profound wound, 94% (72-100%) in the superficial wound, and 71% (42-100%) in plasma. Conclusions. Personalized cefuroxime dosing by repeated weight-dosed (20 mg/kg) intravenous administrations provided homogenous and therapeutic spine tissue exposure across all investigated tissues and plasma in long-lasting spine surgery with hypotensive anaesthesia (up to 11 hours). Thus, personalized cefuroxime dosing may decrease the risk of postoperative spine infection, especially in cases with implant insertion

In Denmark the most common postoperative pathogen is S. aureus (1), sensitive to dicloxacillin. These bacteria can cause a postoperative infection despite using prophylactic antibiotics. Whether the tissue concentration reached is above the minimal inhibitory concentration (MIC) for the pathogens is unknown, and if lower than expected could result in a postoperative infection. Thus a trial was conducted, measuring the actual tissue concentration of dicloxacillin in human muscle and adipose tissue and compared these to the plasma concentration. MIC for dicloxacillin against S. aureus was determined using the broth macrodilution method. Six healthy male volunteers aging 25 to 27 years (body-mass-index; 20–28), were recruited. A CMA63 (Mdialysis, Stockholm, Sweden) catheter was placed in the subcutaneous tissue of the abdomen and in the rectus muscle of the thigh and the volunteers given 2 g dicloxacillin intravenously over 5 minutes. In 10 min intervals for the following 6 hours, samples from blood and Microdialysis fluid (flowrate 5 ml/min) were collected. Recovery was determined in vitro. Plasma was isolated from blood samples. The unbound dicloxacillin was isolated from plasma using filter plates (AcroPrep 30K Omega, Pall Corporation, US) centrifuged for 30 minutes at 1000 × g and 37°C. All samples were analyzed with High Performance Liquid Chromatography. MIC was determined to be 0.125 µg/ml. Average recovery was 73,7 % Maximum concentrations were reached in muscle tissue after a median of 0.5 hours and adipose tissue after 0.8 hours. The geometric mean ration (GMR) of AUC0-6h for adipose tissue compared to plasma was 0.32 [0.15–0.71]. GMR of AUC0-6h for muscle tissue compared to plasma and adipose tissue compared to muscle showed no statistically significant differences. The tissue concentrations were above MIC for 3.4 hours for adipose tissue and 4.1 hours for muscle tissue. The administration of prophylactic dicloxacillin should be given at least 30 minutes prior to incision to ensure maximum tissue concentrations at the onset of surgery. A second dose should be given after 3.4 hours in case of long surgery time. Since the dicloxacillin concentration reached in the adipose tissue is lower than in plasma, it should be investigated whether this difference is more prominent in adipose patients or patients with impaired peripheral circulation, since these patients are at a greater risk of postoperative infections

Residual strain development in biological tissue is believed to result from remodeling in response to repetitive loading. This study hypothesized that differences in in-vivo loading between levels of the bovine tail result in differences in intervertebral disc (IVD) annulus fibrosus (AF) microstructural remodeling. The hypothesis was tested by quantifying tail musculature using clinical computed tomography and tissue microstructure using collagen fiber crimp period, which has previously been correlated with residual strain. Three bovine tail segments (levels c1 through c6) were imaged using a clinical computed tomography (CT) scanner followed by removal of muscle and harvest of IVDs. The discs were frozen, and transverse cryosections were obtained. Additionally, tangential plane cryosections were obtained from the inner and outer zones of the AF. Transverse CT slices corresponding to each joint level thresholded for both disc and muscle tissue and analyzed in MATLAB. First, the centroid of the disc image was calculated to use as an origin. Then the disc area and moments of inertia about the flexion extension axis and lateral bending axis were calculated. Total muscle area was then calculated, along with muscle moments of inertia relative to the disc centroid. All muscle parameters were normalized by those of the corresponding disc. Cryosections were imaged using an inverted light microscope equipped with crossed polarizing filters and a digital camera. A MATLAB routine was used to perform Fourier transform analysis on user selected lines of interest in the transverse micrographs, yielding average fiber crimp period in the inner and outer AF. Micrographs from tangential sections were opened in ImageJ, and fiber orientation angles were measured manually. Muscle moments of inertia were analyzed using a two-way ANOVA with disc level and axis as dependent variables. Normalized muscle area was analyzed with a one-way ANOVA with disc level as a dependent variable. A two-way ANOVA, with disc level and zone (inner versus outer) was used to analyze collagen fiber crimp period and collagen fiber angle. Normalized muscle moment of inertia showed significant effects of both level and axis (p < 0 .001), decreasing at distal levels, and being lower about the flexion-extension axis than the lateral bending axis. Normalized muscle cross section showed a visible, but not significant (p=0.0721) decreasing trend with disc level. Fiber crimp period had significant effects of both level and zone (p < 0 .001), and was significantly longer in the outer zone than inner at all levels. Significant decrease in crimp period at distal levels were seen in the outer AF, but not the inner. While fiber angle was significantly (p < 0 .001) higher in the inner AF (36±6.6°) than outer AF (24±3.5°)), there was no significant effect of level. Fiber crimp period in the AF has previously been correlated with residual circumferential strain, with larger crimp period corresponding to increased residual tension. The present study suggests that at proximal levels of the tail, where peak compressive and bending stresses in the AF (as inferred from normalized muscle area and moments of inertia respectively) are greatest, there is more accumulation of residual strain

Complete or nearly complete disruption of the attachment of the gluteus is seen in 10–20% of cases at the time of THA. Special attention is needed to identify the lesion at the time of surgery because the avulsion often is visible only after a thickened hypertrophic trochanteric bursa is removed. From 1/1/09 to 12/31/13, 525 primary hip replacements were performed by a single surgeon. After all total hip components were implanted, the greater trochanteric bursa was removed, and the gluteus medius and minimus attachments to the greater trochanter were visualised and palpated. Ninety-five hips (95 patients) were found to have damage to the muscle attachments to bone. Fifty-four hips had mild damage consisting of splits in the tendon, but no frank avulsion of abductor tendon from their bone attachments. None of these cases had severe atrophy of the abductor muscles, but all had partial fatty infiltration. All hips with this mild lesion had repair of the tendons with #5 Ticron sutures to repair the tendon bundles together, and drill holes through bone to anchor the repair to the greater trochanter. Forty-one hips had severe damage with complete or nearly complete avulsion of the gluteus medius and minimus muscles from their attachments to the greater trochanter. Thirty-five of these hips had partial fatty infiltration of the abductor muscles, but all responded to electrical stimulation. The surface of the greater trochanter was denuded of soft tissue with a rongeur, the muscles were repaired with five-seven #5 Ticron mattress sutures passed through drill holes in the greater trochanter, and a gluteus maximus flap was transferred to the posterior third of the greater trochanter and sutured under the vastus lateralis. Six hips had complete detachment of the gluteus medius and minimus muscles, severe atrophy of the muscles, and poor response of the muscles to electrical stimulation. The gluteus medius and minimus muscles were sutured to the greater trochanter, and gluteus maximus flap was transferred as in the group with functioning gluteus medius and minimus muscles. Postoperatively, patients were instructed to protect the hip for 8 weeks, then abductor exercises were started. The normal hips all had negative Trendelenburg tests at 2 and 5 years postoperative with mild lateral hip pain reported by 11 patients at 2 years, and 12 patients at 5 years. In the group of 54 with mild abductor tendon damage that were treated with simple repair, positive Trendelenburg test was found in 5 hips at 2 years and in 8 hips at 5 years. Lateral hip pain was reported in 7 hips at 2 years, and in 22 at 5 years. In the group of 35 hips with severe avulsion but good muscle tissue, who underwent repair with gluteus maximus flap transfer, all had good abduction against gravity and negative Trendelenburg tests at 2 and 5 years postoperative, and none had lateral hip pain. Of the 6 hips with complete avulsion and poor muscle who underwent abductor muscle repair and gluteus maximus flap transfer, all had weak abduction against gravity, mildly positive Trendelenburg sign, and mild lateral hip pain at 2 and 5 years postoperative. Abductor avulsion is uncommon but not rare, and is detected during THA only by direct examination of the tendon and removal of the trochanteric bursa. Simple repair of mild abductor tendon damage did not prevent progressive abductor weakness in some hips; and the increase in number of patients with lateral hip pain from 2 to 5 years suggests progressive deterioration. Augmentation of the repair with a gluteus maximus flap appears to provide a stable reconstruction of the abductor muscles, and seemed to restore abductor function in the hips with functioning muscles

Aims

This study explored the shared genetic traits and molecular interactions between postmenopausal osteoporosis (POMP) and sarcopenia, both of which substantially degrade elderly health and quality of life. We hypothesized that these motor system diseases overlap in pathophysiology and regulatory mechanisms.

Painful neuromas may follow traumatic nerve injury. We carried out a double-blind controlled trial in which patients with a painful neuroma of the lower limb (n = 20) were randomly assigned to treatment by resection of the neuroma and translocation of the proximal nerve stump into either muscle tissue or an adjacent subcutaneous vein. Translocation into a vein led to reduced intensity of pain as assessed by visual analogue scale (5.8 (sd 2.7) vs 3.8 (sd 2.4); p < 0.01), and improved sensory, affective and evaluative dimensions of pain as assessed by the McGill pain score (33 (sd 18) vs 14 (sd 12); p < 0.01). This was associated with an increased level of activity (p < 0.01) and improved function (p < 0.01). Transposition of the nerve stump into an adjacent vein should be preferred to relocation into muscle

INTRODUCTION. Mechanical tissue properties of some ligaments and tendons have been described in the literature. However, to our knowledge no data exists describing the tensile properties of the Iliopsoas tendon. The iliopsoas complex is in very close proximity to the hip joint running through the psoas notch from the inner side of the pelvis to the lesser trochanter on the posterior aspect of the proximal femur. The tendon muscle complex wraps around the anterior aspect of the femoral head. Hip joint intervention such as total hip arthroplasty (THA) can interfere with iliopsoas function and contact mechanics, and thereby play a major role in the clinically known condition of anterior hip pain. For computer simulations such as finite element analysis (FEA) precise knowledge of soft-tissue mechanical properties is crucial for accurate models and therefore, the goal of this study was to describe the iliopsoas tensile properties using uniaxial testing equipment. METHODS. Ten iliopsoas tendons were harvested from five specimens (2 male, 3 female; 82.4 yrs ±7.4 yrs) and then carefully cleaned from any fat and muscle tissue. Two freeze clamps were fixed to each end of the tendon sample. The clamps were submerged in liquid nitrogen for 30 seconds to prevent tendon slip and attached to the test frame and load cell via carabiners allowing the tendon to rotate around its long axis. Width, thickness and initial gauge length of each tendon were measured before testing. The test protocol included 10 cycles of preconditioning between 6 N and 60 N at 0.4 mm/s, followed by continuous distraction at 0.4 mm/s until failure. For each tendon the linear stiffness was determined by fitting a straight line to the liner region on the force-displacement curve (Fig. 1). RESULTS. The average linear stiffness of the ten iliopsoas tendons was measured to be 339 N/mm ±81 N/mm and the average failure load resulted in 2154 N ±418 N (Fig. 2). Average width and thickness were determined to be 13.9 mm ±3.2 mm and 3.8 mm ±0.5 mm respectively. The initial gauge length of the ten tendons revealed an average of 56.5 mm ±10.5 mm. CONCLUSION. An average stiffness of 339 N/mm and average failure load of 2154 N was found in our experiments. A trend of increased stiffness and reduced failure load with higher age could be observed. Soft-tissue mechanical properties are dependent on tissue geometry such as cross-sectional area and length and therefore can be variable in comparison with other anatomical structures (e.g. patella tendon). To our knowledge no data has been published on the mechanical properties of iliopsoas tendons and therefore results from this research could be used for future simulation models involving the iliopsoas tendon such as FEA analysis to evaluate the effect of anterior hip pain due to soft-tissue impingement

Backgrounds. The authors present an analysis of 30 cases of total hip prosthesis performed with minimally invasive surgical earlier compares with 30 cases of total hip prosthesis performed surgically with direct side. The intent is to demonstrate the immediate and clear advantages especially in the postoperative surgical minimally invasive front. Methods. The authors present a randomized study of thirty cases of patients undergoing surgery for osteoarthritis of the hip prosthesis with primary surgical minimally invasive front compared to a group of 30 patients undergoing surgery with direct surgical side. All patients had the same cementless implants and instruments with the same surgeon. Patients were then evaluated in four different times: before surgery, immediate postoperative, after 1 week and 4 months after surgery. Results and conclusions. The experience of the authors on the surgical front shows the immediate and clear advantages in postoperative species by saving the muscle tissues, little pain, little bleeding, more quick and easy re-education and rehabilitation

Introduction:. Repair of the arthrotomy is a performed at the end of every total knee arthroplasty (TKA). After the arthrotomy is performed, most surgeons attempt to close the arthrotomy with the medial and lateral edges anatomically approximated. If no landmarks are made prior to performing the arthrotomy however, there is a risk that anatomic approximation may not be obtained. This study looked into the biomechanical changes in stiffness of the knee before and after a medial parapatellar approach repaired with an anatomic, and shifted capsular repair with the medial side of the arthrotomy shifted up or down when repaired to determine if capsular closure may have an effect on the stiffness of the joint. Methods:. Fourteen cadaveric TKA specimens were retrieved through the Medical Education and Research Institute (Memphis TN). For each specimen tested, the skin and muscle tissue was removed, and the femur and tibia were cut transversely 180 mm from the joint center. Specimens were fixed in extension in a custom knee testing platform (Little Rock AR) and subjected to a 10 Nm varus and valgus torque and a 1.5 Nm internal and external rotational torque. The angle at which these moments occurred was recorded, and each test was repeated for 0, 30, 60, and 90 degrees of flexion. After tests were performed on retrieved TKA specimens, a fellowship trained orthopedic surgeon vented the knee capsule by making an incision with a number 10 scalpel blade in a horizontal nature to provide a landmark for anatomic reapproximation. Tests were repeated as before, after which the surgeon performed a standard arthrotomy and repaired it using #0 suture and a neutral alignment. Sutures were cut and the repair was repeated using upward 5 mm shift and downward 5 mm shift of the medial side of the arthrotomy during the repair. All tests were repeated after each repair technique. Any increase or decrease in laxity after capsule repair was referenced to the TKA laxity tested prior to an arthrotomy being performed. Results:. Simply venting the capsule did increase laxity of the TKA in midflexion to varus torque by 3 degrees under the same torque. Otherwise, when the medial limb of the arthrotomy was shifted up during closure by 5 mm, the knee joint tended to be stiffer in flexion compared to the neutral repair measurements under varus torque, while it was closer to the measurements of the neutral or anatomic closure when the medial limb was shifted down during closure. These changes seemed to be seen in flexion more than full extension. Discussion and Conclusion:. Small changes were measured in the stiffness of the joint after venting the capsule and under different degrees of non-anatomic closure. The results stress the fact that the capsule can be measurably tightened during arthrotomy repair and may impact post-operative rehabilitation or range of motion

Introduction:. Soft tissue balancing is a part of every total knee arthroplasty (TKA) surgery. Traditionally, balancing the varus knee has been approached by releasing portions of the medial soft tissue sleeve in a sub-periosteal nature off of the proximal tibia, but this may lead to undue laxity or residual pain about the area the release was performed. More recently, “pie crusting” of the medial soft tissue sleeve has been used to balance the varus knee without compromising the structural integrity of the ligament. This technique may provide advantages over a sub-periosteal release by targeting only medial tight bands that can be palpated with the capsule distracted in 90 degrees of flexion and full extension. This study aims to biomechanically validate the pie crusting technique of the medial soft tissue sleeve and compare the results to those of standard medial releases that have been previously reported. Methods. Six cadaveric TKA specimens were retrieved through the Medical Education and Research Institute (Memphis TN). For each specimen tested, the skin and muscle tissue was removed, and the femur and tibia were cut transversely 180 mm from the joint center. Specimens were fixed in extension in a custom knee testing platform (Little Rock AR) and subjected to a 10 Nm varus and valgus torque. The angle at which these moments occurred was recorded, and each test was repeated for 0, 30, 60, and 90 degrees of flexion. After tests were performed on TKA specimens, a fellowship trained orthopedic surgeon performed “pie crusting,” making alternating stab patterns with a number 11 scalpel blade along the anterior half of the superficial medial collateral ligament (SMCL) or posterior half of the SMCL including the posterior oblique ligament (POL). Three specimens had the anterior capsule pie crusted first and three had the posterior pie crusting performed first, followed by complete pie crusting. After two stages of pie crusting, the medial soft tissue sleeve was released off of the proximal tibia in a sub-periosteal fashion for comparison. Laxity was defined as the angles at which valgus torque equaled 10 Nm. Any increase or decrease in laxity was referenced to the normal TKA laxity. Results:. In full extension, average laxity using complete pie crusting and complete standard ligament release were similar in all flexion angles tested. Pie crusting the anterior half of the medial capsular structures resulted in increased valgus laxity of 3.9 ± 1.6 degrees in 90 degrees of flexion compared to 0.2 ± 2 degrees in full extension. After posterior pie crusting of the medial capsular structures, valgus laxity increased in full extension by 1.8 ± 0.9 degrees with little difference in 90 degrees of flexion. Discussion:. A standard medial collateral ligament release did not cause significantly greater overall valgus laxity compared to pie crusting at any of the flexion angles tested. As previously reported, the anterior half of the SMCL affected laxity in flexion, while the posterior SMCL and POL affected laxity in extension. This knowledge could enable surgeons to make more informed choices when deciding which TKA laxity balancing techniques to use

Soft tissue balancing during Total Knee Arthroplasty (TKA) is a step every surgeon takes during surgery. Coronal and transverse plane mechanical alignment is another parameter that surgeons address during surgery in an attempt to decrease wear and increase longevity. To date, a correlation between laxity, component wear patterns, and alignment of the tibial and femoral implant components has not been established. Theoretically, suboptimal alignment and poor soft tissue balancing should increase polyethylene wear and decrease implant survivorship, contributing to implant loosening and costly revision surgeries. This study utilizes a retrieval program of functioning TKAs obtained at the time of necropsy. By utilizing CT scans, mechanical laxity testing, and polyethylene damage scores, we aimed to determine if any correlation between proper alignment and ligament balancing to polyethylene damage scores exists. Methods:. Computed Tomography (CT) scans were performed on 17 cadaveric knees containing TKAs obtained from the Medical Education and Research Institute (Memphis TN) using a GE Brightspeed scanning system with a 1.25 mm slice thickness. Transverse slices from these scans were used to calculate the femoral and tibial component rotation for each specimen. Component rotation was determined by utilizing previously published methods (Berger et al), and component mismatch was defined as the difference in rotation angles of the femoral and tibial components. After removal of skin, subcutaneous and muscle tissue, the tibia and femur of each leg was cut transversely, and the specimens were mounted in a custom knee testing machine (Little Rock, AR). Specimens were subjected to a 10 Nm varus and valgus torque and a 1.5 Nm internal and external rotation torque. Data was continuously recorded, and the angle or displacement at each torque or force was noted. Each test was performed at full extension and 30, 60, and 90 degrees of flexion. TKA components were then removed from the cadaveric knees, cleaned of PMMA, and visually inspected for wear using a grading system with 10 wear areas on the articulating surface of the polyethylene tibial insert (Hood et al). Scores were assigned based on severity of 7 different degradation characteristics, and were separated based on medial or lateral compartment. The maximum possible total score was 210 for each knee. Results:. The average length of TKA implantation was 10 years. The coronal angle at +10 Nm (varus) moment ranged from 5 to 12 degrees, while the angle under a −10 Nm (valgus) moment ranged from 7 to 11 degrees across 10 specimens. The average component rotational mismatch was 20.5 degrees. The average overall medial wear score was 8.8, while the lateral average was 9.6. Wear scores showed a higher correlation to laxity in the medial compartment than the lateral side (Figures 1 and 2). Discussion:. TKA polyethylene wear shows a relationship to ligament laxity in these specimens. Component mismatch shows a negative correlation to wear scores. If surgeons are able to test for these parameters sooner, it may be possible to prevent unnecessary TKA wear and thus many revision surgeries

Aims

Bone demonstrates good healing capacity, with a variety of strategies being utilized to enhance this healing. One potential strategy that has been suggested is the use of stem cells to accelerate healing.

Currently, there is no animal model in which to evaluate the underlying physiological processes leading to the heterotopic ossification (HO) which forms in most combat-related and blast wounds. We sought to reproduce the ossification that forms under these circumstances in a rat by emulating patterns of injury seen in patients with severe injuries resulting from blasts. We investigated whether exposure to blast overpressure increased the prevalence of HO after transfemoral amputation performed within the zone of injury. We exposed rats to a blast overpressure alone (BOP-CTL), crush injury and femoral fracture followed by amputation through the zone of injury (AMP-CTL) or a combination of these (BOP-AMP). The presence of HO was evaluated using radiographs, micro-CT and histology. HO developed in none of nine BOP-CTL, six of nine AMP-CTL, and in all 20 BOP-AMP rats. Exposure to blast overpressure increased the prevalence of HO.

This model may thus be used to elucidate cellular and molecular pathways of HO, the effect of varying intensities of blast overpressure, and to evaluate new means of prophylaxis and treatment of heterotopic ossification.

Cite this article: Bone Joint J 2015;97-B:572–6