Local infiltration analgesia is a relatively novel technique developed for effective pain control following total knee replacement, reducing requirements of epidural or parenteral post-operative analgesia. The study aimed to investigate the anatomical spread of Local Infiltration Analgesia (LIA) used intra-operatively in total knee arthroplasty (TKA) and identify the nerve structures reached by the injected fluid. Six fresh-frozen cadaveric lower limbs were injected with 180ml of a solution of latex and India ink to enable visualisation. Injections were done according to our standardised LIA technique. Wounds were closed and limbs were placed flat in a freezer at −20°C for two weeks. Limbs were then either sliced or dissected to identify solution locations. Injected solution was found from the proximal thigh to the middle of the lower leg. The main areas of concentration were the popliteal fossa, the anterior aspect of the femur and the subcutaneous tissue of the anterior aspect of the knee. There was less solution in the lower popliteal fossa. The solution was found to reach the majority of the terminal branches of the tibial, fibular and obturator nerves. Overall, there was good infiltration of nerves supplying the knee. The lack of infiltration into the lower popliteal fossa suggests more fluid or a different injection point could be used. The solution that travelled distally to the extensor muscles of the lower leg probably has no beneficial analgesic effect for a TKA patient. This LIA technique reached most nerves that innervate the knee joint which supports the positive clinical results from this LIA technique. However, there may be scope to optimise the injection sites