INTRODUCTION. Standing spinal alignment has been the center of focus recently, particularly in the setting of adult spinal deformity. Humans spend approximately half of their waking life in a seated position. While lumbopelvic sagittal alignment has been shown to adapt from standing to sitting posture, segmental vertebral alignment of the entire spine is not yet fully understood, nor are the effects of DEGEN or DEFORMITY. Segmental spinal alignment between sitting and standing, and the effects of degeneration and deformity were analyzed. METHODS. Segmental spinal alignment and lumbopelvic alignment (pelvic tilt (PT), pelvic incidence (PI), lumbar lordosis (LL), PI-LL, sacral slope) were analyzed. Lumbar spines were classified as NORMAL, DEGEN (at least one level of disc height loss >50%, facet arthropathy, or spondylolisthesis), or DEFORMITY (PI-LL mismatch>10°). Exclusion criteria included lumbar fusion/ankylosis, hip arthroplasty, and transitional lumbosacral anatomy. Independent samples t-tests analyzed lumbopelvic and segmental alignment between sitting and standing within groups. ANOVA assessed these differences between spine pathology groups. RESULTS. There were 183 NORMAL, 216 DEGEN and 92 DEFORMITY patients with significant differences in age, gender, and hip OA grades. After propensity matching for these factors, there were 56 patients in each group (age 63±14, 58% female) [Fig. 1]. Significant differences were noted between spinal pathology groups with regard to changes from standing to sitting alignment with regard to NORMAL vs DEGEN vs DEFORMITY groups in PT (13.93° vs −11.98° vs − 7.95°; p=0.024), LL (21.91° vs 17.45° vs 13.23°; p=0.002), PI-LL (−22.32° vs −17.28° vs −13.18°; p<0.001), SVA (−48.99° vs −29.98° vs −32.12°; p=0.002), and TPA(−16.35° vs −12.69° vs −9.64; p=0.001). TK (−2.08° vs −2.78° vs −2.00°, p=0.943) and CL (−3.84° vs −4.14° vs −3.57°, p=0.621) were not significantly different across spinal pathology groups [Fig. 2]. NORMAL patients had overall greater mobility in the lower lumbar spine from standing to sitting compared to DEGEN and DEFORMITY patients. L4-L5 (7.50° vs 5.23° vs 4.74°, p=0.012) and L5-S1 (6.96° vs 5.28° and 3.69°, p=0.027). There were no significant differences in change in alignment from standing to sitting at the upper lumbar levels or lower thoracic levels between the three groups [Fig. 3]. CONCLUSION. The lower lumbar spine provides the greatest sitting to standing change in lumbopelvic alignment in normal patients. Degeneration and deformity of the spine significantly reduces the mobility of the lower lumbar spine and PT. With lumbar spine degeneration and flatback deformity, relatively more alignment change occurs at the upper lumbar spine and thoracolumbar junction