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Orthopaedic Proceedings
Vol. 91-B, Issue SUPP_III | Pages 465 - 465
1 Sep 2009
Izal I Ripalda P Acosta V Ochoa I Bea J Doblaré M Aranda P Escribano R Mora G Valentí J Gallego G Recalde I Gòmez-Ribelles J Pròsper F
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Hyaline cartilage is a support tissue with a poor capacity to self repair. In the last years, tissue engineering and cell therapy have focused its efforts in the development of scaffolds that may support the differentiation and the implantation of mesnechymal stem cells (MSC) in the site of lesions performed in femoral cartilage. Among synthetic materials used for the construction of these scaffolds, poly(L-lactic acid) (PLLA) is a suitable option, since some studies have offered promising results. The use of PLLA, nevertheles has an important handicap, as cell seeding easily results in a non uniform distribution and a poor density of cells, wich have been proposed as key steps for the differentiation of MSCs to chondrocytes. In our work we have cultured sheep MSCs, and proved its potentiallity by differentiation to chondrocytes in micromass culture. PLLA scaffolds 1 mm thick and 6 mm in diameter were characterized by determining their porosity and their mechanical properties, and subsequently were used to assay the seeding of MSCs. We measured efficiency and retention by quantification of DNA, and density and distribution by light microscopy of paraffin sections. Our results describe a simple technique of cell seeding by aspirating cells with a syringe that achieves a uniform distribution and a high density of cells. Finally 3D seeded MSCs were cultured with condrogenic medium containing TGF-β3 for 21 days and results analyzed by massons trichrome staining in paraffin embedded sections.


Orthopaedic Proceedings
Vol. 91-B, Issue SUPP_II | Pages 323 - 323
1 May 2009
Valentí A Mora G Valentí JR
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Introduction: Anterior cruciate ligament (ACL) surgery is ever more frequent and commonly has a satisfactory outcome.

Purpose: To assess whether platelet-enriched gel has positive effects on immediate postoperative evolution and on the final clinical result.

Materials and methods: We carried out a prospective, randomized study by alternatively selecting 50 patients. The control group (without gel) comprised 25 patients and the Gel group (with platelet gel) also contained 25 patients; both groups were homogeneous in terms of age and sex. The same anesthesia, surgery and analgesia protocol was used in all cases and hospital discharge was 24–48 hours postoperatively. Pain assessment was carried out by detection of inflammation (pre and post patellar perimeter) and PCR (at 24 hours and 7 days). Clinical results were assessed by means of IKDC; radiological studies were performed using plain x-rays and MRI.

Results: No statistically significant differences were found (p< 0.05) in all the data analyzed, although there is a trend towards less inflammation in the group treated with gel, both clinically and analytically. There were no significant differences in the functional results of surgery and the MRI showed an increased intensity of signal and greater homogeneity in T2 in the gel group plasties.

Conclusions: The use of platelet gel in ACL surgery could have a beneficial effect on inflammatory and analytical parameters, but not on clinical results.