Introduction Leg length inequalities (LLIs) are a common finding in every orthopaedic practice. They can be classified into anatomical and functional LLIs. LLIs can e.g. cause gait and balance disabilities, low back pain and functional scoliosis of the spine. In patients with a total hip replacement a higher rate of aseptic loosening of the prosthesis was found when LLIs were present (Gurney 2002). Until today LLIs are treated statically by wooden blocs, which are placed under the shorter extremity, until the pelvis is levelled. However, the correction of LLIs should also be evaluated dynamically to examine the influence of correction onto the spine and pelvis during gait. Therefore, we seek to evaluate in this pilot study the influence of simulated LLIs on spine and pelvis during gait. Methods A total of 30 healthy subjects (17 females & 13 males) with an average age of 24.4 years were measured in this study. First, LLIs (1 to 4 cm) were simulated with the subjects standing on a simulation platform, which height could be controlled, as previously described (Betsch 2012). In addition, a specially designed sandal with different insole heights (1 to 4 cm) was used to simulate LLIs under dynamic condition while subjects were walking on a treadmill. Changes in pelvic position and spinal posture caused by the LLIS were measured using a rasterstereographic system (Formetric 4D motion, Diers International GmbH, Germany). All data were checked for Gaussian distribution by the Chi square test. Student t-tests were used to check for differences between the LLIs. The level of significance was set at p
Aim of the study was to evaluate if abrasion-arthroplasty (AAP) and abrasion-chondroplasty (ACP) leads to a release of mesenchymal stem cell (MSC) like cells from the bone marrow to the joint cavity where they probably differentiate into a chondrogenic phenotype. Cartilage demage is a sever problem in our aging society. About 5 million people only in Germany are affected. Osteoathritis is a degeneration of cartilage caused by aging or traumata 50 % of the people over 40 have signs of osteoarthritis. But the ability of self-regeneration of cartilage is strongly limited. There are different approaches to therapy osteoathritic lesions. Arthroscopic treatment of OA includes bone marrow stimulation technique such as abrasion arthroplasty (AAP) and microfracturing (MF). Beside the support of chondrocyte progenitor cells the environment is also important for the commitment to chondrocytes. Therefore insulin-like growth factor-1 (IGF-1) and transforming growth factor beta-1 (TGF-β1) are important factors during the regeneration process. In the present study we characterised the heamarthrosis and the released cells after AAP and its ability to differentiate into the chondrocyte lineage. Postoperative haemarthrosis was taken 5, 22 or 44 hours after surgery. 7.5 mg Dexamethasone (Corticosteroid) was administered into the knee joint to prevent postoperative inflammation. Mononuclear cells were isolated from haemarthrosis from the drainage bottle by ficoll density gradient centrifugation. The isolated cells were characterised using fluorescence-activated cell-sorting (FACS) analysis for characteristic markers of MSC such as CD 44, 73, 90, 105. After expanding cells were cultured in a pellet culture. After 3 weeks, histochemistry and immunohistochemistry against Sox9, collagen II and proteoglycan were performed. The release of IGF1, BMP4 and BMP7 was analysed in haemarthrosis serum by ELISA and Luminex technology.Introduction
Material and Methods
