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Orthopaedic Proceedings
Vol. 87-B, Issue SUPP_II | Pages 169 - 169
1 Apr 2005
Haridas S Curtis C Caterson B Evans R Dent C
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Aim: To evaluate the functional outcome of patients following intra-osseous suturing for repair of distal biceps tendon ruptures, using the Mayo scoring system. Subsequent analysis of mRNA expression; in the ruptured biceps tendons was performed.

Methods: We operated on 8 patients who had ruptured their biceps tendon. The average ages of the patients were 36 (Range 22–50). The technique involved using intrasosseous suturing via a single anterior skin crease incision. The functional outcome of these patients was scored by using the Mayo elbow performance score. The average follow-up was 7 months. (Range 5–8 months). The tendons were processed for RNA isolation and reverse -transcription – polymerase chain reaction (RT-PCR).

Results: The average subjective assessment (pain and function) of these patients was 63/70 (Range 57–68). The average objective assessment (motion and stability) was 24/30 (Range 22–27). The overall average was 87/100. None of the patients had any complications postoperatively. Our results showed that in the samples of ruptured biceps tendon there was mRNA expression of ECM structural components, especially aggrecan and the small proteoglycans biglycan and decorin. Interestingly, these samples also showed a high expression for the enzymes commonly involved in articular cartilage degradation and turnover, the aggrecanases (ADAMTS-4 and ADAMTS-5) and the matrix metalloproteinases (MMP-3 and MMP-13).

Conclusion: We demonstrated that intrasosseous suturing via a single anterior incision, in-patients with ruptured biceps tendons could provide a good functional outcome. This technique should therefore be considered as one of the surgical options in the management of this condition. We know clinically that patients can rupture their biceps tendon either due to trauma if not due to degenerative conditions. In our study we wanted to know if the subset of patients how ruptured their tendons traumatically had any pre-existing degenerative conditions leading on to the rupture compared to the normal subjects. Interestingly our study has shown that there is mRNA expression of degradative enzymes (aggrecanases and MMPs) in the samples of ruptured biceps tendon. Furthermore, our samples also showed mRNA expression for factors involved in the inflammatory response. In conclusion, mRNA expression of the factors involved in degradation and inflammation may suggest a phenotype that predisposes the biceps tendon to rupture, although further studies are required in order to investigate this.


Orthopaedic Proceedings
Vol. 87-B, Issue SUPP_II | Pages 161 - 161
1 Apr 2005
Haridas* S Curtis C Caterson B Evans R Dent C
Full Access

Aim: To study mRNA expression in ruptured biceps tendon.

Methods: Our study was carried out in the University College of Medicine. We took the biceps tendon of 5 patients who had traumatic ruptures. The age of the patients ranged from 35–53. The tendons were processed for RNA isolation and reverse-transcription-polymerase chain reaction (RT-PCR) carried out in order to investigate the mRNA gene expression in ruptured biceps tendon of extra cellular matrix (ECM) components (e.g. proteoglycans and collagens); ECM degradative components (e.g. aggrecanases and MMPs); inflammatory components (e.g. cytokines and cyclooxygenases); and factors involved in the apoptotic response.

Results: Our results showed that in the samples of ruptured biceps tendon there was a good mRNA expression of ECM structural components, especially aggrecan and the small proteoglycans biglycan and decorin. Interestingly, these samples also showed a high expression for the enzymes commonly involved in articular cartilage degradation and turnover, the aggrecanases (ADAMTS-4 and –5) and the matrix metalloproteinases (MMP-3 and –13). As has been recently reported for Achilles tendon rupture (Cetti et al, 2003), an inflammatory reaction was also observed in these ruptured bicep tendons with expression of the inflammatory cytokines IL-1α and TNFα and the enzyme cyclooxygenase-2.

Conclusion: We know clinically that patients can rupture their biceps tendon either due to trauma if not due to degenerative conditions. In our study we wanted to know if the subset of patients who ruptured their tendons traumatically had any pre-existing degenerative conditions leading on to the rupture compared to the normal subjects. Interestingly our study has shown that there is mRNA expression of degradative enzymes (aggrecanases and MMPs) in the samples of ruptured biceps tendon. Whether these mRNA levels equate to increased enzyme activity of these molecules warrants further investigation. Furthermore, our samples also showed mRNA expression for factors involved in the inflammatory response. In conclusion, mRNA expression of the factors involved in degradation and inflammation may suggest a phenotype that predisposes the bicep tendon to rupture, although further studies are required in order to investigate this further.