Tissue inhibitors of metalloproteinases (TIMPs) are the endogenous inhibitors of the zinc-dependent matrix metalloproteinases (MMP) and A disintegrin and metalloproteinases (ADAM) involved in extracellular matrix modulation. The present study aims to develop the TIMPs as biologics for osteoclast-related disorders. We examine the inhibitory effect of a high affinity, glycosyl-phosphatidylinositol-anchored TIMP variant named ‘T1PrαTACE’ on receptor activator of nuclear factor kappa-Β ligand (RANKL)-induced osteoclast differentiation.Aims
Methods
The prevention of osteoporotic fractures is a global problem. Key to this strategy is efficient identification of ‘at risk’ patients in order to address the osteoporosis pandemic, including the identification of previously sustained fractures. GP practices are now integrating touch screens as a method of registering patients' attendance for an appointment, so all ages of patients are becoming familiar with this channel of communication. Our touch screen patient administered questionnaire system intends to provide an effective solution. The Virtual Research Integration Collaboration (VRIC) framework supports the integration of basic science and clinical research. It enables the management of research lifecycles by integrating scientific approaches with everyday work practice in a virtual research environment (VRE). ‘Catch Before a Fall’ (CBaF) is a clinical research project using VRIC, using a dedicated interface, co-designed by orthopaedic surgeons and basic scientists, adapted for sensory and IT impaired subjects to capture such information, since approximately 75% of registered over 65 year olds visit their GP each year.Background and objectives
Methods
gold compound was reduced in the presence of lysozyme to form Lys-AuNPs or citrate-stabilized AuNPs were functionalized with mercaptopropionic acid (MPA) to produce carboxylic acid terminated AuNPs which were mixed with lysozyme. Both solutions were characterized with transmission electron microscopy, ultraviolet-visible spectroscopy, circular dichroism spectroscopy (CD), and enzymatic assays. Next, AuNPs were prepared on 99.5% titanium foil discs (n=32) through electroless deposition. Deposition parameters were modified to create two groups of discs with different average diameters of AuNPs, measured by scanning electron microscopy. Some discs from both groups also underwent treatment with MPA. All discs were treated with lysozyme and the adsorbed amounts and activities of lysozyme were examined with micro BCA and enzymatic assays.
