Despite promising results have shown by osteogenic cell-based demineralized bone matrix composites, they need to be optimized for grafts that act as structural frameworks in load-bearing defects. The aims of this study is attempt to assess the effects of laser perforations on osteoinduction in cortical bone allografts. Sixteen wistar rats were divided into two groups according to the type of structural bone allograft; the first: partially demineralized only (Donly) and the second: partially demineralized laser-perforated (DLP). Trans-cortical holes were achieved by Er:YAG laser at a wave length of 2.94 µm in four rows of three holes approximated cylindrical holes 0.5 mm in diameter, with centres 2.5 mm apart. Histologic and histomorphometric analysis were performed at 12 weeks.Objectives
Methods
Despite promising results have shown by osteogenic cell-based demineralized bone matrix composites, they need to be optimized for grafts that act as structural frameworks in load-bearing defects. The purpose of this experiment is to determine the effect of bone marrow mesenchymal stem cells seeding on partially demineralized laser-perforated structural allografts that have been implanted in critical femoral defects. Thirty-two wistar rats were divided into four groups according to the type of structural bone allograft; the first: partially demineralized only (Donly), the second: partially demineralized stem cell seeded (DST), the third: partially demineralized laser-perforated (DLP), and the fourth: partially demineralized laser-perforated and stem cell seeded (DLPST). Trans-cortical holes were achieved in four rows of three holes approximated cylindrical holes 0.5 mm in diameter, with centres 2.5 mm apart. P3 MSCs were used for graft seeding. Histologic and histomorphometric analysis were performed at 12 weeks.Background
Materials and Methods
