Prosthetic joint infections represent complications connected to the implantation of biomedical devices, they have high incidence, interfere with osseointegration, and lead to a high societal burden. The microbial biofilm, which is a complex structure of microbial cells firmly attached to a surface, is one of the main issues causing infections. Biofilm- forming bacteria are acquiring more and more resistances to common clinical treatments due to the abuse of antibiotics administration. Therefore, there is increasing need to develop alternative methods exerting antibacterial activities against multidrug-resistant biofilm-forming bacteria. In this context, metal-based coatings with antimicrobial activities have been investigated and are currently used in the clinical practice. However, traditional coatings exhibit some drawbacks related to the insufficient adhesion to the substrate, scarce uniformity and scarce control over the toxic metal release reducing their efficacy. Here, we propose the use of antimicrobial silver-based nanostructured thin films to discourage bacterial infections. Coatings are obtained by Ionized Jet Deposition, a plasma-assisted technique that permits to manufacture films of submicrometric thickness having a nanostructured surface texture, allow tuning silver release, and avoid delamination. To mitigate interference with osseointegration, here silver composites with bone apatite and hydroxyapatite were explored. The antibacterial efficacy of silver films was tested in vitro against gram- positive and gram-negative species to determine the optimal coatings characteristics by assessing reduction of bacterial viability, adhesion to substrate, and biofilm formation. Efficacy was tested in an in vivo rabbit model, using a multidrug-resistant strain of Staphylococcus aureus showing significant reduction of the bacterial load on the silver prosthesis both when coated with the metal only (>99% reduction) and when in combination with bone apatite (>86% reduction). These studies indicate that IJD films are highly tunable and can be a promising route to overcome the main challenges in orthopedic prostheses.

Prosthetic joint infections represent complications connected to the implantation of biomedical devices, they have high incidence, interfere with osseointegration, and lead to a high societal burden. The microbial biofilm, which is a complex structure of microbial cells firmly attached to a surface, is one of the main issues causing infections. Biofilm- forming bacteria are acquiring more and more resistances to common clinical treatments due to the abuse of antibiotics administration. Therefore, there is increasing need to develop alternative methods exerting antibacterial activities against multidrug-resistant biofilm-forming bacteria. In this context, metal-based coatings with antimicrobial activities have been investigated and are currently used in the clinical practice. However, traditional coatings exhibit some drawbacks related to the insufficient adhesion to the substrate, scarce uniformity and scarce control over the toxic metal release reducing their efficacy. Here, we propose the use of antimicrobial silver-based nanostructured thin films to discourage bacterial infections. Coatings are obtained by Ionized Jet Deposition, a plasma-assisted technique that permits to manufacture films of submicrometric thickness having a nanostructured surface texture, allow tuning silver release, and avoid delamination. To mitigate interference with osseointegration, here silver composites with bone apatite and hydroxyapatite were explored. The antibacterial efficacy of silver films was tested in vitro against gram- positive and gram-negative species to determine the optimal coatings characteristics by assessing reduction of bacterial viability, adhesion to substrate, and biofilm formation. Efficacy was tested in an in vivo rabbit model, using a multidrug-resistant strain of Staphylococcus aureus showing significant reduction of the bacterial load on the silver prosthesis both when coated with the metal only (>99% reduction) and when in combination with bone apatite (>86% reduction). These studies indicate that IJD films are highly tunable and can be a promising route to overcome the main challenges in orthopedic prostheses.

Both total joint arthroplasty (TJA) and Alzheimer's Disease (AD) are prevalent in elderly populations. It is the goal of this study to determine if the presence of implant metals originating from TJA correlates with the onset with higher implant metal content in the brain and AD pathology.

Tissue samples from four brain regions of 701 (229 with TJA) participants from an ongoing longitudinal cohort study (Rush Memory and Aging Project) was analyzed including the inferior-temporal-cortex (ITC), which is associated with early onset of AD. Implant metal (Co, Cr, Mo, Ti, Al) content was determined by ICP-MS. Comparisons were conducted between the no-TJA-group and a TJA group. Due to the higher likelihood of Co release the TJA group was further differentiated in a THA (N=146) and a TKA/TSA (N=83) group. Diffuse and neuritic amyloid plaques and phosphorylated tau were assessed and summarized as standard measures of AD pathology. We used separate linear regression models adjusted for age, sex, education, and APOɛ4-status for the associations of all metals (log-transformed) with global AD pathology, amyloid plaques, and phosphorylated tau.

The THA group had higher cobalt content across all brain regions (p=0.003) and within the ITC (p=0.051) compared to the no-TJA group, whereas the TKA/TSA group did not. Across all tissue samples, Co was associated with higher amyloid load (β=0.35, p=0.027), phosphorylated tau (β=0.47, p=0.011), and global AD pathology (β=0.19, 0.0004) in the ITC. The presence of TJA itself was not associated with AD pathology.

We showed that only Co content was higher within the ITC in persons with THA. We found among all tested metals that Co was consistently associated with AD pathology. Although we found an association of cobalt with AD pathology, the cross-sectional nature of this study does not allow the determination of cause and effect.

Prosthetic joint infections represent complications connected to the implantation of biomedical devices. Bacterial biofilm is one of the main issues causing infections from contaminated orthopaedic prostheses. Biofilm is a structured community of microbial cells that are firmly attached to a surface and have unique metabolic and physiological attributes that induce improved resistance to environmental stresses including toxic compounds like antimicrobial molecules (e.g. antibiotics). Therefore, there is increasing need to develop methods/treatments exerting antibacterial activities not only against planktonic (suspended) cells but also against adherent cells of pathogenic microorganisms forming biofilms. In this context, metal-based coatings with antibacterial activities have been widely investigated and used in the clinical practice. However, traditional coatings exhibit some drawbacks related to the insufficient adhesion to the substrate, scarce uniformity and scarce control over the toxic metal release reducing the biofilm formation prevention efficacy. Additionally, standardized and systematic approaches to test antibacterial activity of newly developed coatings are still missing, while standard microbiological tests (e.g. soft-agar assays) are typically used that are limited in terms of simultaneous conditions that can be tested, potentially leading to scarce reproducibility and reliability of the results.

In this work, we combined the Calgary Biofilm Device (CBD) as a device for high-throughput screening, together with a novel plasma-assisted technique named Ionized Jet Deposition (IJD), to generate and test new generation of nanostructured silver- and zinc-based films as coatings for biomedical devices with antibacterial and antibiofilm properties. During the experiments we tested both planktonic and biofilm growth of four bacterial strains, two gram-positive and two gram-negative bacterial strains, i.e. Staphylococcus aureus ATCC 6538P, Enterococcus faecalis DP1122 and Escherichia coli ATCC 8739 and Pseudomonas aeruginosa PAO1, respectively. The use of CBD that had the only wells covered with the metal coatings while the biofilm supports (pegs) were not sheltered allowed to selectively define the toxic effect of the metal release (from the coating) against biofilm development in addition to the toxic activity exerted by contact killing mechanism (on biofilms formed on the coating). The results indicated that the antibacterial and antibiofilm effects of the metal coatings was at least partly gram staining dependent. Indeed, Gram negative bacterial strains showed high sensitivity toward silver in both planktonic growth and biofilm formation, whereas zinc coatings provided a significant inhibitory activity against Gram positive bacterial strains. Furthermore, the coatings showed the maximal activity against biofilms directly forming on them, although, Zn coating showed a strong effect against biofilms of gram-positive bacteria also formed on uncoated pegs.

We conclude that the metal-based coatings newly developed and screened in this work are efficient against bacterial growth and adherence opening possible future applications for orthopedic protheses manufacturing.

Aims

Metal allergy in knee arthroplasty patients is a controversial topic. We aimed to conduct a scoping review to clarify the management of metal allergy in primary and revision total knee arthroplasty (TKA).

Aims

This study aims to enhance understanding of clinical and radiological consequences and involved mechanisms that led to corrosion of the Precice Stryde (Stryde) intramedullary lengthening nail in the post market surveillance era of the device. Between 2018 and 2021 more than 2,000 Stryde nails have been implanted worldwide. However, the outcome of treatment with the Stryde system is insufficiently reported.

Objectives

Preclinical data showed poly(methyl methacrylate) (PMMA) loaded with microsilver to be effective against a variety of bacteria. The purpose of this study was to assess patient safety of PMMA spacers with microsilver in prosthetic hip infections in a prospective cohort study.

Since 2010, there has been a sharp decline in the use of metal-on-metal joint replacement devices due to adverse responses associated with the release of metal wear particles and ions in patients. Surface engineered coatings offer an innovative solution to this problem by covering metal implant surfaces with biocompatible and wear resistant materials. The present study tests the hypothesis whether surface engineered coatings can reduce the overall biological impact of a device by investigating recently introduced silicon nitride coatings for joint replacements. Biological responses of peripheral blood mononuclear cells (PBMNCs) to Si3N4 model particles, SiNx coating wear particles and CoCr wear particles were evaluated by testing cytotoxicity, inflammatory cytokine release, oxidative stress and genotoxicity.

Clinically relevant wear particles were generated from SiNx-on-SiNx and CoCr-on-CoCr bearing combinations using a multidirectional pin-on-plate tribometer. All particles were heat treated at 180°C for 4 h to destroy endotoxin contamination. Whole peripheral blood was collected from healthy donors (ethics approval BIOSCI 10–108, University of Leeds). The PBMNCs were isolated using Lymphoprep (Stemcell) and incubated with particles at various volumetric concentrations (0.5 to 100 µm3 particles/cell) for 24 h in 5% (v/v) CO2 at 37°C. After incubation, cell viability was measured using the ATPlite assay (Perkin Elmer); TNF-alpha release was measured by ELISA (Invitrogen); oxidative stress was measured using H2DCFDA (Abcam); and DNA damage was measured by comet assay (Tevigen). The results were expressed as mean ± 95% confidence limits and the data was analysed using one-way ANOVA and Tukey-Kramer post-hoc analysis.

No evidence of cytotoxicity, oxidative stress, TNF-alpha release, or DNA damage was observed for the silicon nitride particles at any of the doses. However, CoCr wear particles caused cytotoxicity, oxidative stress, TNF-alpha release and DNA damage in PBMNCs at high doses (50 µm3 particles per cell). This study has demonstrated the in-vitro biocompatibility of SiNx coatings with primary human monocytic cells. Therefore, surface engineered coatings have potential to significantly reduce the biological impact of metal components in future orthopaedic devices.

Objectives

Opening wedge high tibial osteotomy (HTO) is an established surgical procedure for the treatment of early-stage knee arthritis. Other than infection, the majority of complications are related to mechanical factors – in particular, stimulation of healing at the osteotomy site. This study used finite element (FE) analysis to investigate the effect of plate design and bridging span on interfragmentary movement (IFM) and the influence of fracture healing on plate stress and potential failure.

Over the last 50 years, biomaterials, prostheses and implants saved and prolonged the life of millions of humans around the globe. The main clinical complications for current biomaterials and artificial organs still reside in an interfacial mismatch between the synthetic surface and the natural living tissue surrounding it. Today, nanotechnology, nanomaterials and surface modifications provides a new insight to the current problem of biomaterial complications, and even allows us to envisage strategies for the organ shortage. Advanced tools and new paths towards the development of functional solutions for cardiovascular clinical applications are now available. In this talk, the potential of nanostructured metallic degradable metals to provide innovative solutions at medium term for the cardiovascular field will be depicted. Focus will be on Fe-based biodegradable metals with exceptional resistance, ductility and elasticity, for pushing innovative vascular applications. The intrinsic goal of this talk is to present an extremely personal look at how biodegradable metals can impact materials, surfaces and interfaces, and how the resulting unique properties allowed biomedical functional applications to progress, from their introduction, to the promising future that biodegradable metals may or may not hold for improving the quality of the life of millions worldwide.

Background. Mechanisms underlying implant failure remain incompletely described, though the presence of macrophage-mediated inflammatory reactions is well documented. Hypoxia has a critical role in many diseases and is known to be interdependent with inflammation. Metals used for joint replacements have also been reported to provoke hypoxia-like conditions. In view of this, we aim to investigate hypoxia-associated factors in aseptic loosening and osteoarthritis with a focus on macrophages. Methods. Western blotting, calorimetric assay, haematoxylin-eosin staining, immunohistochemistry, double-immunofluorescence and transmission electron microscopy were performed on capsular tissue obtained from patients undergoing primary implantation of a total hip replacement for osteoarthritis and from patients undergoing revision surgery for aseptic loosening to investigate the presence of hypoxia-associated factors. Results. Tissues from patients with osteoarthritis and aseptic loosening showed the presence of inflammatory cells, many of which were macrophages as confirmed with CD68 immunostaining. In aseptic loosening, macrophages containing metal particles were present in clusters. This was observed both at the light and electron microscopic levels. Under the electron microscope, endothelial cells appeared to be hypertrophied and some showed signs of degeneration. The presence of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and nitric oxide was demonstrated by western blotting and colorimetric assay. Macrophages were the predominant cell type to release HIF-1α, VEGF, inducible nitric oxide synthase (iNOS). This was confirmed by double-immunofluorescence showing co-localization of HIF-1α, VEGF, iNOS with the macrophage marker CD68. Endothelial cells were stained for endothelial nitric oxide synthase as assessed by immunohistochemistry. Conclusion. This study demonstrates the release of hypoxia-associated factors by macrophages. The presence of hypoxia-associated factors in both, osteoarthritis and aseptic loosening suggest that hypoxia may be a factor underlying both pathologic conditions. This study was supported by research grant (NMRC/CNIG/1147/2016) from National Medical Research Council (NMRC), Singapore

Major bone loss involving the acetabulum can be seen during revision THA due to component loosening, migration or osteolysis and can also occur as a sequela of infected THA. Uncemented highly porous ingrowth acetabular components can be used for the reconstruction of the vast majority of revision cases, especially where small to mid-sized segmental or cavitary defects are present which do not compromise stable mechanical support by the host bone for the cup after bone preparation is complete. A mechanically stable and near motionless interface between the host bone and the implant is required over the initial weeks post-surgery for bone ingrowth to occur, regardless of the type of porous surface employed. As bone deficiency increases, the challenge of achieving rigid cup fixation also increases, especially if the quality of the remaining host bone is compromised. A stepwise approach to enhanced fixation of the highly porous revision acetabular component is possible as follows:

Maximise Screw Fixation. Use of a limited number of screws in the dome only (as routinely occurs with a cluster hole design) is inadequate, except for primary arthroplasty cases or very routine revision cases with little or no bone loss and good bone quality. Otherwise an array of screws across the acetabular dome and continuing around the posterior column to base of the ischium is strongly recommended. This can help prevent early rocking of the cup into a more vertical position due to pivoting on dome screws used alone, via cup separation inferiorly in zone 3. A minimum of 3 or 4 screws in a wide array are suggested and use of 6 or more screws is not uncommon if bone quality is poor or defects are large.

Cement the Acetabular Liner into the Shell. This creates a locking screw effect, which fixes the screw heads in position and prevents any screws from pivoting or backing out.

Acetabular Augments (vs Structural Allograft). When critical segmental defects are present which by their location or size preclude stable support of the cup used alone, either a structural allograft or highly porous metal augment can provide critical focal support and enhance fixation. Highly porous metal augments were initially developed as a prosthetic allograft substitute in order to avoid the occasional graft resorption and loss of fixation sometimes seen with acetabular allograft use.

Cup-Cage Construct. If one or more of the above strategies are used and fixation is deemed inadequate, it is possible to add a ½ or full acetabular cage “over the top” of the acetabular component before cementing a polyethylene liner in place. The full cup cage construct can be used for maximal fixation in cases of pelvic dissociation, alone or in combination with the distraction method as described by Paprosky. Use of a ½ cage is technically simpler and requires less exposure than a full cage, but still greatly enhances rigidity of fixation when transverse screws into the ilium are combined with standard screws in the cup including vertically into the dome.

These techniques used in combination with highly porous tantalum implants have allowed durable fixation for the full range of reconstructive challenges and bone defects encountered. Newer 3-D printed titanium highly porous materials have recently been introduced by multiple manufacturers as a potential alternative that may be more cost effective, but these implants and materials will require clinical validation over the years ahead.

Pathological assessment of periprosthetic tissues is important, not only for diagnosis, but also for understanding the pathobiology of implant failure. The host response to wear particle deposition in periprosthetic tissues is characterised by cell and tissue injury, and a reparative and inflammatory response in which there is an innate and adaptive immune response to the material components of implant wear. Physical and chemical characteristics of implant wear influence the nature of the response in periprosthetic tissues and account for the development of particular complications that lead to implant failure, such as osteolysis which leads to aseptic loosening, and soft-tissue necrosis/inflammation, which can result in pseudotumour formation. The innate response involves phagocytosis of implant-derived wear particles by macrophages; this is determined by pattern recognition receptors and results in expression of cytokines, chemokines and growth factors promoting inflammation and osteoclastogenesis; phagocytosed particles can also be cytotoxic and cause cell and tissue necrosis. The adaptive immune response to wear debris is characterised by the presence of lymphoid cells and most likely occurs as a result of a cell-mediated hypersensitivity reaction to cell and tissue components altered by interaction with the material components of particulate wear, particularly metal ions released from cobalt-chrome wear particles.

Cite this article: Professor N. A. Athanasou. The pathobiology and pathology of aseptic implant failure. Bone Joint Res 2016;5:162–168. DOI: 10.1302/2046-3758.55.BJR-2016-0086.

Introduction. Metals used for total knee arthroplasty (TKA) are well known for their good biocompatibility, but may be a source of a release of metal ions that can be a cause of local and systemic adverse effects, aseptic loosening, and hypersensitivity reactions. One of the major difficulties in performing TKA is the selection of implants for patients who are preoperatively diagnosed as subject to metal sensitivity. Alternative solutions in cases of hypersensitivity are implants without metal constituents or metallic implants treated with a non-sensitive surface process. The aim of this study was to evaluate clinical results in patients who had been preoperatively diagnosed with metal sensitivity and who subsequently were provided with the zirconia-ceramic LFA-III TKA, and with a minimum 5-year follow-up. Methods. Five patients (8 knees) with metal sensitivity underwent TKA using cemented zirconia-ceramic LFA-III implants. The LFA-III implant (KYOCERA Medical Co., Japan) is composed of a zirconia ceramic femoral component and a titanium-alloy tibial component with a polyethylene insert. All patients were female andthe average age at the time of surgery was 76.1 years. The average follow-up time was 7.2 years. Clinical and radiographic assessments were conducted with the Knee Society scoring system. Results. No patients except one who had palmoplantar pustulosis preoperatively presented systemic or local dermatitis after surgery. The mean preoperative range of motion of 97.6 degrees improved to a mean of 110.7 degrees at the time of the most recent follow-up. The mean postoperative knee and function scores were 77.1 and 66.9, respectively. Subtle periprosthetic radiolucencies were found in 2 knees after the surgery. Discussion. The zirconia-ceramic LFA-III TKA has performed well over a 5-year period in patients with metal hypersensitivity. Although this implant has a metal tibial component made of titanium, no systemic or local adverse events related to metal hypersensitivity were recorded. Ceramic implants can be an attractive alternative solution for patients suffering from hypersensitivity reactions to metals

The long term biological effects of wear products following total hip arthroplasty (THA) are unclear. However, the indications for THA are expanding, with increasingly younger patients undergoing the procedure.

This prospective, randomised study compared two groups of patients undergoing THA after being randomised to receive one of two different bearing surfaces: metal-on-polyethylene (MoP) n = 22 and metal-on-metal (MoM) n = 23. We investigated the relationship between three variables: bearing surface (MoP vs MoM), whole blood levels of chromium (Cr) and cobalt (Co) and chromosomal aberrations in peripheral lymphocyte pre-operatively and at one, two and five years post-surgery.

Our results demonstrated significantly higher mean cobalt and chromium (Co and Cr) blood levels in the MoM group at all follow-up points following surgery (p < 0.01), but there were no significant differences in the chromosomal aberration indices between MoM and MoP at two or five years (two years: p = 0.56, p = 0.08, p = 0.91, p = 0.51 and five years: p = 0.086, p = 0.73, p = 0.06, p = 0.34) for translocations, breaks, loss and gain of chromosomes respectively. Regression analysis showed a strong linear relationship between Cr levels and the total chromosomal aberration indices in the MoM group (R² = 0.90016), but this was not as strong for Co (R² = 0.68991). In the MoP group, the analysis revealed a poor relationship between Cr levels and the total chromosomal aberration indices (R² = 0.23908) but a slightly stronger relationship for Co (R² = 0.64292). Across both groups, Spearman’s correlation detected no overall association between Co and Cr levels and each of the studied chromosomal aberrations. There remains no clear indication which THA bearing couple is the most biocompatible, especially in young active patients. While THA continues to be very successful at alleviating pain and restoring function, the long-term biological implications of the procedure still require further scrutiny.

Cite this article: Bone Joint J 2015;97-B:1183–91.

Highly porous metal surfaces have transformed acetabular revision surgery by providing (1) enhanced friction which potentially provides greater primary fixation, (2) enhanced bone ingrowth potential, (3) enhanced screw fixation options. These characteristics have led many surgeons to use these devices routinely in acetabular revision and have led to an expansion of the indications for porous uncemented hemispherical cups in acetabular revision. Mid-term results suggest that the historical indications for hemispherical cups in revision surgery can be moderately expanded with some implants with these characteristics. In a recent study of 3448 revision total hip arthroplasties, we found porous tantalum cups had a statistically lower revision rate than other materials/designs. Highly porous metals also have provided the options of metal augments to fill selected bone defects—which can both enhance cup fixation and manage bone loss simultaneously. A number of different highly porous metals are now available, and how each will perform is not yet known.

Highly porous metal shells may be used in combination with highly porous metal augments to make up for segmental bone deficiency. Examples will be shown. Finally, highly porous metal shells may be used as a “cup-cage” combination to provide extra initial cup mechanical stability in extreme cases. Examples will be shown.

Introduction. In recent years, there has been a growing interest in bioresorbable metals. Orthopaedic components made from these materials do not require removal by secondary surgery, and offer superior load bearing capability compared to the existing biodegradable polymers. Research on bioresorbable metals have largely focused on alloys based on a subset of the Mg-Zn-Ca ternary system [1, 2], which are pre-existing elements inside the human body. Cytocompatibility assessments of these alloys have reported no signs of inflammation or adverse cellular reactions [2-4]. Rather than designing for longevity, bioresorbable metals rely on their tendency to corrode in a controlled manner. Hence, controlling their corrosion rates is of utmost importance. In the present work, we have explored the effect of compositional variation on the properties of the Mg-Zn-Ca amorphous metals. Subsequent characterisations are performed to assess their suitability as a bioresorbable material. Materials and Methods. A mixture of pure elements and master alloys, namely magnesium, zinc, calcium, and Mg-Ca master alloy, were melted in an induction furnace, followed by injection casting to produce the amorphous metallic samples. Pure magnesium (crystalline) was also used in the subsequent characterisation tests for comparison. The thermophysical properties of the as-cast amorphous metals were characterized using x-ray diffraction (XRD) and differential scanning calorimetry (DSC). The biocorrosion performance was assessed by a combination of immersion, potentiodynamic polarisation (PDP) and hydrogen evolution studies. These tests were conducted in cell media, with a sodium bicarbonate buffer, at 37°C and pH 7.4 in a humidified CO. 2. atmosphere. Results and Discussion. A range of amorphous metal compositions, from Mg-rich to Ca-rich, were successfully produced. XRD confirmed that the alloys were amorphous. Subsequent characterisation tests revealed that minor alterations in composition were not detrimental to thermophysical properties; however, the critical casting size and corrosion rates were much more sensitive to alloy chemistry. In comparison, the Mg-rich alloys have superior corrosion resistance, whereas the Ca-rich alloys have improved thermophysical properties, thereby allowing them to undertake more complex thermoplastic forming processes. Conclusion. We have successfully produced amorphous metals with a range of corrosion resistance and thermophysical properties. The combination of biocompatible elements, superior corrosion resistance and reduced hydrogen evolution, make these amorphous metals more suitable for use as bioresorbable orthopaedic components than their crystalline counterparts. Acknowledgements. The authors would like to thank the Australian Research Council (ARC) for partial funding of this work via the ARC Centre of Excellence for Design in Light Metals (CE0561574)

Introduction. Are there really ‘conventional’ bearings, offering more security and less risk than the ‘alternative’ bearings that feature in the programme?. Alternative, when used as an adjective has 2 meanings:. offering or expressing a choice, as in several alternative plans. different from or functioning outside the usual or conventional:. eg alternative newspaper, alternative rock music, alternative medicine. This paper reviews the elements that make up the bearing couples available today in the developed world, and tests each bearing against these meanings. Materials. what are the alternatives?. The materials available today fall into the following broad families:. Metals. Stainless Steel and Cobalt-Chromium Alloy, are the dominant metals available. There is no variation in the Steel, but the characterisation of the Co-Cr does vary. Several manufacturers use different carbide content for the femoral and acetabular components, and different processes. One has been withdrawn from the market, and others may be at risk of this, although it is not the material itself that seems to be the main issue. Ceramics include alumina and zirconia ceramics. Alumina has been available unchanged for over 40 years, although delta ceramic (a zirconia toughened alumina) has only been available unchanged since 2001, making in available for 10 years. Polymers. a huge range of polyethylenes are now available, with different individual claims. All claim superior wear resistance, and oxidation resistance. More than 20 unique products are available in the EU, each with a proprietary formula giving individual characteristics. Coatings and surface treatments. these are now available today from many companies, who either ceramicise the surface of cobalt chrome or titanium with titanium nitride, or use oxinium (a proprietary product from a single company). Bearing couples. what are the alternatives Symmetric and Asymmetric bearings are currently offered. Symmetric bearings are available for Ceramic on Ceramic and Metal on Metal bearings only. Asymmetric bearings are available with metals, including metal on poly, and metal on peek. Ceramics can couple with metal or polymers. Bearing Sizes. Larger than 32mm should be considered ‘alternative’. The larger metal bearings have seen the start of crevice corrosion at the taper between titanium and Co-Cr, and even between different Co-Cr alloys. This new class of complication seems to be unique to metal femoral heads. Bearing-stem compatibility. Larger metal on metal head bearings have brought an entirely new world of complications. The choices of trunion are mainly twofold: the 12/14 tapers which differ significantly between products, and the V40 taper still used by one manufacturer. Neither was designed for use with a larger diameter head. Conclusions. The use of the word ‘alternative’ implies a ‘standard’ or conventional bearing. Ceramic bearings have changed least, have been immune from the metallosis and crevice corrosion seen with large ball Metal head whether bearing on metal or polyethylene. They also have reassuring long term results. In 2011 they should be considered the standard bearing for the young and active patient. The large diameter metal on highly cross-linked poly bearings should now be considered ‘alternative’

A major concern in metal on metal bearings has been the elevated serum concentrations of cobalt and chromium. Recent papers have suggested that metal hypersensivity in a few cases could cause periprostetic lymphocyte accumulation leading prosthetic loosening.

To measure the lymphocyte activation and proliferation in vitro by re-exposure of the cells to cobalt, chromium, nickel and titanium. To correlate the lymphocyte assay data to the serum concentration of metals and plasma cytokines.

A prospective clinical study with the ASR (DePuy) and ReCap (Biomet) resurfacing hip implants. Blood samples were collected one and two years postoperatively, lymphocytes were isolated by density gradient centrifugation, cultured in a medium containing the patient’s serum and exposed to metal salts.

Cells were analyzed by flow cytometry, evaluating number, viability, size and CD69 activation.

A negative control and a positive control (phytohaemagglutinine) were included in the assay, and the responses to the metals were calculated in proportion to controls. 11 patients were assessed at one and two years follow up, 16 patients were assessed only at two years.

Serum chromium and cobalt were measured preoperatively, six months, one year and two years postoperatively by graphite furnace absorptiometry. Plasma cytokines were measured by multiplexed immunoassay.

In the assay the negative and positive controls gave the expected responses.

When exposed to metals no response was found in the lymphocytes in any patients.

There were no difference in response between one and two years.

The results seems to indicate that the metal hypersensitivity is a rare condition in metal on metal arthroplasty. The results indicate that the method can be used to monitor hypersensitivity to implant metals.

Replacement of damaged or diseased tissues with permanent metal implants based on stainless steel, cobalt chrome and titanium alloys has been at the forefront of classical biomaterials research and the orthopaedic medical device industry for decades. Biodegradable polymers have also reached the market but often have limited capacity in load bearing orthopaedic applications due to their low stiffness and poor mechanical properties. The development of biodegradable metals based on magnesium (Mg) could be heralded as a major breakthrough in the field of orthopaedic surgery. Degradable implants eliminate the time and cost associated with a secondary surgery to remove hardware, and reduces the period the implant is exposed to instability, fibrous encapsulation, stress shielding and inflammation. The metabolism of Mg and its excretion via the kidneys is a natural physiological process that is well understood, however, controlling the rapid degradation of Mg biomaterials in vivo is a major challenge yet to be resolved for the safe and effective use of Mg in orthopaedic implants.

In this study, we describe a novel manufacturing method for fabricating Mg/Mg alloy implants, as well as the development of an in vitro method for screening Mg/Mg alloy degradation rate by considering both their electrochemical corrosion behaviour and biological characteristics.

A range of Mg alloys with varying amounts of calcium (0.8–28%) and zinc (3–10%) were cast and then machined into Ø4mm and 15mm discs for biocompatibility (HETCAM) and parallel in vitro testing. Alloys were placed in various simulated body fluid (SBF) solutions in vitro (7–28 days) to determine effect of alloy composition on degradation rate. These potentiostatic and potentiodynamic tests were designed to simulate, to varying degrees, the in vivo environment, with the crucial factors (e.g. temperature, pH, serum proteins, CO2 level) controlled to ensure consistency across the test methods. The mechanisms of corrosion on the Mg/Mg alloy microstructure and the effect of protein adsorption all played key roles in dictating the corrosion of alloys in vitro. Specifically the inclusion of physiological levels of serum proteins decreased the corrosion rate up to 600% over more standard SBF solutions described in literature.

This work provides an improved understanding of the effects of corrosion variables on Mg alloys, while making major steps towards deciding the most appropriate screening tests for new alloys for their use as a biomedical material prior to moving to in vivo animal studies.