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The Journal of Bone & Joint Surgery British Volume
Vol. 83-B, Issue 1 | Pages 144 - 147
1 Jan 2001
Mayr-Wohlfart U Kessler S Puhl W Günther KP Knöchel W

Since bone morphogenetic proteins (BMPs) are highly homologous, we investigated the hypothesis that recombinant BMP-4 of the genome of Xenopus laevis (rxBMP-4) may influence the proliferation or differentiation of human primary osteoblast-like cells (HPOC), as occurs with recombinant human BMP (rhBMP-2).

HPOC were incubated in the presence of either rxBMP-4, rhBMP-2 or basic fibroblast growth factor (rh-bFGF). The last two were used as positive controls and are known to induce differentiation or proliferation of HPOC, respectively.

rxBMP-4 (50 ng/ml and 100 ng/ml) induced a differentiation of HPOC to almost the same extent as rhBMP-2, whereas the addition of rh-bFGF, applied in the same concentration, failed to have any influence on cell differentiation. rh-bFGF however, provoked an increase in cell proliferation of up to 150% when compared with non-stimulated HPOC, while rhBMP-2 and rxBMP-4 had no such effect.

Our results indicate an equipotent effect of rhBMP-2 and rxBMP-4 obtained from Xenopus laevis on the differentiation and proliferation of human primary osteoblast-like cells.


The Journal of Bone & Joint Surgery British Volume
Vol. 78-B, Issue 6 | Pages 997 - 997
1 Nov 1996
HYDER N KESSLER S JENNINGS AG DE BOER PG


The Journal of Bone & Joint Surgery British Volume
Vol. 78-B, Issue 3 | Pages 499 - 500
1 May 1996
Hyder N Kessler S Jennings AG De Boer PG