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Orthopaedic Proceedings
Vol. 91-B, Issue SUPP_I | Pages 6 - 6
1 Mar 2009
de Palma L marinelli M pavan M orazi A
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Introduction: Pathological conditions, which determine human atrophy, are numerouses and heterogeneous.

Experimental studies prove that these different pathological conditions use common enzymatic pathways leading muscle atrophy. In every catabolic conditions where there is proteolyses’s increase, this one happens in association with up-regulation of two specific genes of skeletal muscle atrophy. These genes, MuRf1 (muscle ring finger-1) and MAFbx (muscle atrophy F-box), encode ubiquitin ligases. These ligases bind and mediate ubiquitination of myofibrillar proteins for subsequent degradation during muscle atrophy.

The aim of our study is to obtain a better understanding of human muscle physiopathology in atrophy by use of histochemistry and immunolocalisation of MuRF-1 and MAFbx.

Patients and Methods: 15 patients, amputated at third distal or proximal leg because of different acute or chronic pathology, were divided in two group. Group A: 12 elderly patients (mean age 72 years) amputated for vascular diseases (8) and complication of a diabetic foot (4). Group B: 3 young patients involved in car accident (mean age 25) amputated for limb’s acute arterial insufficiency. Gastrocnemius muscle biopsy specimens were obtained for all the patients, after that the informed consent was obtain, for histochemical (haematossilineosin), and immunohistochemical (anti- MuRf1, anti- MAFbx) analysis.

Results: Histochemistry: Group A: skeletal muscle showed a decrease in fiber size in cross-sectional area and fiber length with adipose tissue. Group B: light skeletal muscle structural alteration. Immuno-histochemistry: in group A, in muscular drawings, polyclonal antibodies direct against MuRf1 and MAFbx had stained muscle biopsy specimens. Muscle fiber cells showed MuRf1 and MAFbx subsarcolemmatic immunoreactivity and weakly immunoreactivity of the extracellular matrix. We noticed no positivity to anti- MuRf1 and anti- MAFbx less in sections from group B muscle biopsy specimens and in sections in which were present tissue muscle degeneration with replacement of adipose tissue.

Conclusion: The pathological results supported the concept that MuRf1 and MAFbx appeared to be regulatory peptide in cellular pathology that conduce to muscular atrophy. Our data support the hypothesis that different pathological conditions use common enzymatic pathways leading muscle atrophy.

The demonstration that the muscle-specific proteins MAFbx and MuRF1 are upregulated in multiple pathological conditions of skeletal muscle atrophy it is critical to continue studying the cellular pathways to discover promising targets for the development of effective new treatments for skeletal muscle disease.


Orthopaedic Proceedings
Vol. 88-B, Issue SUPP_III | Pages 418 - 418
1 Oct 2006
Moschini A Gigante A Verdenelli A Ulisse S Ricci M De Palma L
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Forty patients with subcutaneous rupture of the Achilles tendon were enrolled in a prospective study and randomised to two groups: group A treated with open surgery with Kessler-type suture, and group B treated by percutaneous tenorraphy (Tenolig®) under ultrasound control. The follow-up included an objective and a subjective (SF-12) clinical evaluation at 4, 12 and 24 months, ultrasonography at the same time points, and isokinetic muscle performance tests at 12 months. There were no significant differences between the two groups at clinical and ultrasound evaluation except for a greater ankle circumference in group B (p< 0.01) at 12 months; peak torque and total work isokinetic tests did not differ significantly in the two groups nor between involved and uninvolved side. At 24 months data show similar clinical and ultrasonographic results with both techniques, leading us to prefer percutaneus tenorraphy under ultrasound control owing to the attendant advantages of local anaesthesia in day surgery, decreased risk of skin complications, reduced surgical time, faster functional recovery, and greater patient compliance.