header advert
Results 1 - 1 of 1
Results per page:
Orthopaedic Proceedings
Vol. 101-B, Issue SUPP_12 | Pages 59 - 59
1 Oct 2019
BACTERIOPHAGE-DERIVED LYSIN COMBINATION WITH VANCOMYCIN DEMONSTRATES SUPERIOR ANTIMICROBIAL POTENTIAL IN MURINE DAIR MODEL OF PERIPROSTHETIC JOINT INFECTION (PJI)
Sosa B Niu Y Turajane K Staats K Suhardi V Carli A Fischetti V Bostrom MPG Yang X
Full Access
View article

Introduction

PJI is a devastating complication following total joint arthroplasty. In this study, we explore the efficacy of a bacteriophage-derived lysin, PlySs2, against in-vitro biofilm on titanium implant surfaces and in an acute in-vivo murine debridement antibiotic implant retention (DAIR) model of PJI.

Methods

In-vitro: Xen 36 S. aureus biofilm was grown on Ti-6Al-4V mouse tibial implants for 1 day or 5 days and subsequently exposed to growth media, 1000× minimal inhibitory concentration (MIC) Vancomycin, or 5× MIC PlySs2. Implants were sonicated and analyzed for Colony Forming Units (CFU).

In-vivo: A Ti-6Al-4V implant was inserted into the proximal tibia of C57BL/6J mice (n=21). All mice received 104 CFU inoculation of Xen 36 S. aureus to the knee joint capsule and the infection was permitted 5 days to progress. On day 5 the mice were separated into three groups (n=7/group): (1) no further surgical intervention (control group), (2) irrigation and debridement (I&D) with saline, (3) I&D with 2mg/mL PlySs2. No implant-exchange was performed to mimic a debridement, antibiotic, and implant retention (DAIR) therapeutic strategy. All mice were sacrificed at day 10.