This study aimed to investigate time-dependent gene expression
of injured human anterior cruciate ligament (ACL), and to evaluate
the histological changes of the ACL remnant in terms of cellular
characterisation. Injured human ACL tissues were harvested from 105 patients undergoing
primary ACL reconstruction and divided into four phases based on
the period from injury to surgery. Phase I was <
three weeks,
phase II was three to eight weeks, phase III was eight to 20 weeks,
and phase IV was ≥ 21 weeks. Gene expressions of these tissues were
analysed in each phase by quantitative real-time polymerase chain
reaction using selected markers (collagen types 1 and 3, biglycan,
decorin, α-smooth muscle actin, IL-6, TGF-β1, MMP-1, MMP-2 and TIMP-1).
Immunohistochemical staining was also performed using primary antibodies
against CD68, CD55, Stat3 and phosphorylated-Stat3 (P-Stat3). Objectives
Methods
The purpose of this study is to introduce our technique of free hand screw insertion for scaphoid fractures and clarify the indications of this procedure. From 1988 to date, we performed this method in 86 cases (75 males and 11 females). Ages ranged from 11 to 73 years (av.: 29). There were 24 cases of acute stable type, 46 of acute unstable and 16 of delayed fibrous union. Screws used were original Herbert screws in 48, other cannulated type screws in 38. Using an image intensifier, from a small skin incision over the scaphotrapezium joint, a Kirschner wire is inserted to stabilize the fracture temporarily. The wire is pulled volarward to rotate the scaphoid and a second wire is inserted along the intended line of the screw. With the original Herbert screw, after removing the wire, the screw is inserted free-hand. With the other cannulated screws, the second wire is used as guide pin. Results of 82 cases with follow-up times over 6 months were reviewed. In one case, bony fusion was achieved but revealed symptomatic malunion. In two cases, bony fusion was not achieved. In one of them, an additional bone graft was performed, and good bony union was achieved. In the remaining 79 cases, good bony fusion and good clinical results were achieved. The best indication for this method is an acute unstable fracture. For acute stable fractures, we recommend this method for three types of patients: those who cannot accept long term immobilization, those who desire to return to athletic activities as soon as possible, and those who also have another fracture in the forearm. It can also be used in cases of delayed fibrous union when good alignment can be achieved and a bone graft is unnecessary.
Vascularized bone grafts (VFG) have brought great benefits in the field of reconstruction of the lower extremity. However, complications such as fracture of the grafted fibula and delayed union are sometimes seen. Not only to prevent these complications but also for stability after fracture of the grafted fibula, the Ilizarov external fixator is a very useful option. We report here the clinical results of cases treated by VFG combined with Ilizarov external fixator for reconstruction of the lower extremity. We have performed 53 vascularized fibula transfers to reconstruct lower extremities. An Ilizarov external fixator was used for the initial immobilization in 7 (2 femur, 5 tibia) and for delayed union or fracture of the grafted fibula in 2 cases of congenital pseudoarthrosis of the tibia. All patients achieved good bone reconstruction. All are able to walk without a brace except for one congenital case. The average period to achieve bony fusion was 13 months in femur cases, 6 months in adult tibia cases and 2 months in congenital cases. The average periods to walk without a brace were 14 months, 8 months and 10 months respectively. However, it took 9 months and 28 months to achieve bony union in the cases with delayed union or fracture of the grafted fibula. In the reconstruction of the lower extremities using VFG, the determining factor in method selection is whether sufficient mechanical support is available. An Ilizarov external fixator for immobilization permits the patient to walk as soon as possible. Dynamization from this semi-rigid external fixator causes bone hypertrophy and improved incorporation of the graft.