Introduction

Autologous Chondrocyte Implantation (ACI) is an effective surgical treatment for chondral defects. ACI involves arthrotomy for cell implantation. We describe the development of an intra-articular injection of cultured MSC, progressing from in-vitro analysis, through animal model, clinical and radiological outcome at five years follow up

Gel-based autologous chondrocyte implantation (ACI) over the years have shown encouraging results in repairing the articular cartilage. More recently, the use of cultured mesenchymal stem cells (MSC) has represented a promising treatment option with the potential to differentiate and restore the hyaline cartilage in a more efficient way. This study aims to compare the clinical and radiological outcome obtained in these two groups.

Twenty-eight consecutive symptomatic patients diagnosed with full-thickness cartilage defects were assigned to two treatment groups (16 patients cultured bone marrow-derived MSC and 12 patients with gel-type ACI). The MSC group patients underwent microfracture and bone marrow aspiration in the first stage and injection of cultured MSC into the knee in the second stage. Clinical and radiological results were compared at a minimum follow up of five years

There was excellent clinical outcome noted with no statistically significant difference between the two groups. Both ACI and MSC group showed significant improvement of the KOOS, Lysholm and IKDC scores as compared to their preoperative values and this was maintained at 5 years follow up. The average MOCART score for all lesions was also nearly similar in the two groups. The mean T2* relaxation-times for the repair tissue and native cartilage were 27.8 and 30.6 respectively in the ACI group and 28 and 29.6 respectively in the MSC group.

Use of cultured MSC is less invasive, technically simpler and also avoids the need for a second surgery as compared to an ACI technique. With similar encouraging clinical results seen and the proven ability to restore true hyaline cartilage, cultured MSC represent a favorable treatment option in articular cartilage repair.

The success of uncemented arthroplasty depends on the achievement and maintenance of implant stability. Despite the use of modern instrumentation to obtain an accurate implant fit during total knee replacement, small gaps often remain visible at the bone-prosthesis interface on high quality fluroscopically-assisted radiographs. Although the clinical significance of these gaps is unclear, their presence delays bony fixation of the implant.

In uncemented total hip arthroplasty, hydroxyapatite costing has been used to enhance early stability of the implant: bony apposition has been shown to occur rapidly even in the presence of a small gap between the implant and the bone. In addition, recent RSA (Radio-stereo-photogrammateric analysis) studies have shown reduced micromotion and enhanced implant stability with hydroxyapatite coating of both hip and knee prostheses.

The following study was designed to observe and investigate the phenomenon of ‘gap-healing’ around hydroxyapatite coated uncemented total knee prostheses.

Over a 15-month period a hydroxyapatite coated uncemented total knee prosthesis was implanted in 99 patients undergoing 108 primary knee arthroplasties. The patients were prospectively reviewed at regular intervals with an average follow up of 18 months and a minimum of 12 months. The implant-bone interface was evaluated by obtaining serial fluroscopically-assisted radiographs.

On the immediate postoperative radiographs, small gaps between the implant and bone were seen in most knee. These gaps were visible on average in 2.16 AKS (American Knee Society)zones per knee. Most of the gaps were seen in Femoral zones 2,3,5 and Tibial zones 1 & 4. The majority of the gaps were under 1mm depth. Gaps> 2mm were seen only in 6 patients. Healing of the gaps was first seen at 3 months postoperatively, the average number of zones involved per knee dropping to 1.54. There was good evidence of ‘gap healing’ occurring at all the bone-implant interface zones. At the end of the first postoperative year, only 0.8 zones per knee were involved.2mm gaps remained visible in 3 patients.

In animal experiments, hydroxyapatitie coated porous surfaces have shown an increased the rate of bone ingrowth for as many as 52 weeks after implantation. In our study, progressive bone ingrowth and gap-healing has been observed beyond this period, the average involved zones on 2 –year radiographs being 0.4 per knee.

During the study period, the American knee score improved from 39.52 preoperatively to 89.97 at 1 year postoperatively. No relation was found between the clinical scores and the presence or absence of gaps on follow-up radiographs.

This study demonstrates the phenomenon of ‘gap-healing’ following uncemented hydroxyapatitie coated primary total knee arthroplasty in an unselected group of patients. Gaps under 1 mm at the implant –bone interface heal readily. Healing of gaps> 2mm is less predictable.

We describe the survival at 10 years of 134 consecutive JRI Furlong H.A.-coated uncemented total hip replacements.

The follow-up was a minimum of 10 years (10.5 to 12.0 years).

Patients were assessed by clinical and radiological means. We used Merle d’Aubigné and Postel clinical score.

Radiological assessments identified radio lucent lines, spot welding, pedestal formation and migration in order to assess fixation and stability of the femoral stem according to Engh’s criteria.

DeLee and Charnley zones were used to assess loosening of the ace tabular cup. Subsidence, migration and cup-angle were also measured.

The criteria for failure was revision or impending revision due to either pain, septic or aseptic loosening.

The mean results of the Merle d’Aubigné and Postel score were 7.4 pre-op. and 15.9 post-op.

None of the acetabular cups in this series were revised.

No femoral stem was revised for loosening.

Six patients were lost to follow-up. Of the 128 femoral stems reviewed, only 1 was revised for periprosthetic fracture following a fall.

Total knee replacements and high tibial osteotomies are commonly performed orthopaedic operations with low complication rates. Both of these procedures involve surgery in close proximity to the popliteal artery with the use of power tools and sharp instruments. The behaviour of the popliteal artery during knee flexion, in particular the change in distance between itself and the posterior tibial cortex, is poorly understood. Many previous studies have been on stiff embalmed knees or with the patient lying supine, so as to subject the popliteal artery to an anterior pull from gravity.

We used duplex ultrasonography on 100 healthy knees to determine the distance of the popliteal artery from the posterior tibial surface at 0 and 90 degrees of flexion. One observer was used throughout. At 1–1.5cm below the joint line, we found the artery was closer to the posterior tibial surface in 24% of knees when the knee was flexion. This was also the case for 15% of knees at 1.5–2cm below the joint line. These two levels were chosen as they represent the usual positions for the tibial cuts performed in total knee replacement and tibial osteotomy. We provide an anatomical account to help explain our findings using cadaveric dissections, arteriography and static MRI studies. The first of our explanations for this posterior movement of the artery is the increase in the antero-posterior thickness of the popliteus muscle during knee flexion. We also observed a posterior pull on the popliteal artery from the sural vessels.

6% of the knees had a high branching anterior tibial branch. We highlight this anatomical variant as an example of an extremely vulnerable vessel. We review the existing literature regarding the popliteal artery dynamics, and conclude that 90 degrees of knee flexion is the safer position for tibial procedures, but repeat the warning that the surgeon must still take great care.