This was a double-blind randomised controlled study. The objective of this study was to determine the cause of post-injection pain after peri-articular steroid injection. Approval for this study was granted by the hospital’s Ethics Committee. Selection criteria included all patients undergoing a peri-articular injection under the care of the senior author. Patients who elected to be in this study gave their consent following a detailed explanation of the study and provision of a patient information leaflet. The enrolled patients were randomised into one of two groups. Group A received a standard triamcinolone acetonide injection mixed with bupivicaine. Group B patients received triamcinolone acetonide without the preservative part of the drug and bupivicaine. Both the patient and the surgeon were unaware which group the patient was selected to be in. Patients’ scores were recorded using visual analogue scales and pain severity scores prior to injection and 4 days following injection. Inflammatory signs were also recorded at 4 days post-procedure. A total of 52 patients were enrolled. Pain scores reduced by 46% in group A and 43% in group B. Inflammatory signs occurred in 26% less cases when group B was compared with group A, however this was not statistically significant.

Dupuytren’s contracture is characterised by abnormal fibroblast proliferation and extracellular matrix deposition in the palmar fascia. Fibroblast proliferation and matrix deposition in connective tissues are regulated by cytokines. A number of cytokines including transforming growth factor beta (TGFβ), basic fibroblast growth factor (bFGF), platelet derived growth factor (PDGF) and epidermal growth factor (EGF) are known to have potent anabolic effects on connective tissue. The aim of this study was to investigate the role played by anabolic cytokines in the pathogenesis of Dupuytren’s disease.

Twelve specimens of Dupuytren’s contracture and six control specimens of palmar fascia obtained from patients undergoing carpal tunnel release were cultured using a serumless method under standard conditions for 72 h. Levels of TGFβ-1, bFGF, PDGF and EGF in the medium were estimated using an enzyme linked immunoabsorbent assay technique.

Neither Dupuytren’s tissue nor control palmar fascia produced any EGF. The mean (±S.D.)levels of bFGF, PDGF and TGFβ-1 produced by cultured palmar fascia were: 1270 ± 832, 74 ± 24, < 7, and for Dupuytren’s tissue were 722 ± 237, 139 ± 76.6, 645 ± 332, respectively. The levels of PDGF and TGFβ-1 were significantly higher in Dupuytren’s tissue.

PDGF is produced in increased amounts by Dupuytren’s tissue. This may contribute to the fibroblast proliferation and increased ECM deposition observed in this condition. TGFβ-1 is not produced by normal palmar fascia but is produced in large amounts by Dupuytren’s tissue. The major physiologic role of TGFβ-1 is to stimulate formation of fibrous tissue. It plays a major role in wound healing and also in pathological conditions where fibrosis is a prominent feature. Inappropriate production of TGFβ-1 in the palmar fascia in Dupuytren’s disease may play a central role in initiating and stimulating the abnormal fibroblast proliferation and collagen synthesis seen in this condition.